ATUL TRIPATHI
@rcop.in
Associate Professor
Ravishankar College of Pharmacy
EDUCATION
MAster of Pharmacy, Doctor of Philosophy
RESEARCH, TEACHING, or OTHER INTERESTS
Pharmacology, Toxicology and Pharmaceutics, Pharmaceutical Science, Drug Discovery, General Pharmacology, Toxicology and Pharmaceutics
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Atul Tripathi and Amber Vyas
A and V Publications
Objectives: The aim of the present study was to compare the efficacy of a dual and single drug loaded nano-liposomal formulation of Amphotericin B and Fluconazole for the treatment of visceral leishmaniasis with plain drugs. Methods: We have formulated nano-liposomes (200-250 nm) from Amphotericin B and Fluconazole using dry film hydration method and have tested their efficacy on promastigotes and amastigotes of Leishmania donovani strain. Physicochemical characterization, entrapment study, stability study, in-vitro release study, in-vitro macrophagic uptake studies (Confocal microscopy) and in-vitro antileishmanial activity were evaluated for various formulations containing Amphotericin B and Fluconazole. Results: The in-vitro cellular uptake confocal studies revealed that NR-loaded AmpB + Flu nanoliposomes have enhanced cellular uptake of formulation. The in-vitro inhibition of promastigotes and amastigotes with liposome containing both Amphotericin B and Fluconazole was significantly more than with liposomes containing individual drugs. The IC50 and CC50 of AmpB + Flu nanoliposomes against promastigotes was found to be 3.308μg/mL and 73.48μg/mL respectively, while the IC50 against axenic and intramacrophagic amastigotes was found to be 3.412 and 3.7028μg/mL respectively. Conclusion: In conclusion, Liposomal formulation containing both Amphotericin B and Fluconazole had significantly greater efficacy than conventional combination and other formulation with individual drugs. Current dual drug loaded formulation may have a favourable safety profile, and if production costs are low, it may prove to be a feasible alternative to currently available therapy after in-vivo testing.
Atul Tripathi, Manisha Tandon, Amol Chandekar, Nilam Soni, and Neeraj Upmanyu
Informa UK Limited
ABSTRACT The antioxidant and anthelmintic activities of Luffa cylindrica leaf extracts in ethanol, methanol, and chloroform were investigated. The antioxidant activity was determined by 2,2-diphenyl-picrylhydrazyl (DPPH) and superoxide scavenging assay; the anthelmintic activity was tested against the Indian earthworm (Pheretima posthuma). The methanolic and chloroform leaf extracts showed in vitro antioxidant activity comparable to the standard antioxidant (ascorbic acid); the anthelmintic activities of the ethanolic extract was comparable with the standard drug mebendazole.
Amber Vyas, Bina Gidwani, Atul Tripathi, and Preeti Dhruve
A and V Publications
Jobs plot or continuous variation method is used to estimate the stoichiometry in cyclodextrin complexation. Cyclodextrin complexation is well known as widely acceptable technique for improving the poor physicchemical properties of BCS class II and class IV drugs. The stoichiometry of the inclusion complex is given by the number of guest and host molecules present in the supramolecular complex/inclusion system. The most common stoichiometry is 1: 1, implying the inclusion of a single guest molecule. Now-a-days jobs plot is replaced by phase-solubility study. Jobs plot deals with spectral methods mainly for molecular modelling study of drugs and bioactives.