Genetic Structure of Cucumber Mosaic Virus From Natural Hosts in Nigeria Reveals High Diversity and Occurrence of Putative Novel Recombinant Strains Oluropo A. Apalowo, Adedapo O. Adediji, Olusegun S. Balogun, Temitope I. Fakolujo, Joy M. Archibong, et al. Frontiers in Microbiology, 2022 Cucumber mosaic virus (CMV, Bromoviridae: Cucummovirus), one of the most widespread plant viruses with several hosts, causes huge losses in yield quality and quantity. The occurrence of various CMV strains and high genetic diversity within the virus complicate its management. We describe the population structure of CMV in Nigeria using partial RNA1 and RNA3 gene sequences from three natural hosts: pepper (Capsicum annuum), tomato (Solanum lycopersicum), and watermelon (Citrullus lanatus). One hundred and six leaf samples were obtained from 16 locations across Nigeria, and specific primers were used to amplify the two gene fragments using PCR. Twenty-four samples tested positive for CMV using RNA1 primers, and amplicons were sequenced from 12 isolates, revealing 82.94–99.80% nucleotide and 85.42–100% amino acid sequence similarities within the population. The partial RNA3 fragment, corresponding to the complete coat protein (CP) gene, was sequenced from seven isolates, with 95.79–97.90% and 98.62–100% nucleotide and amino acid intrapopulation similarities, respectively. The isolates belonged to subgroup IB and formed distinct phylogenetic clusters in both gene sets, indicating putative novel strains. Recombination signals, supported by phylogenetic inferences, were detected within the RNA1 dataset (P ≤ 0.05) and identified a recombinant isolate within the Nigerian sequences. No recombination was detected within the CP genes. Population genetics parameters established high diversity within the Nigerian population compared to other isolates worldwide, while selection pressure estimates revealed the existence of negative selection in both gene sets. Although CMV subgroup IB strains were postulated to originate from Asia, this study reveals their prevalence across several hosts from different locations in Nigeria. To our knowledge, this is the first comprehensive description of a recombinant CMV subgroup IB isolate from West Africa, which has implications for its robust detection and overall management.
Control of Root-Knot Nematode (Meloidogyne incognita) in Tomato (Solanum lycopersicum) Crop Using Siam Weed (Chromolaena odorata) Compost Manure Stephen A. Abolusoro, Aruna Olasekan Adekiya, Charity Aremu, Sunday Ige, Nkechi B. Izuogu, et al. Journal of Horticultural Research, 2020 A field experiment was conducted at Landmark University Omu-Aran, Nigeria from June to November 2017 and repeated at the same time in the year 2018 on a nematode infested soil to evaluate the effects of different amounts of Siam weed compost on the performance of root-knot nematode (RKN) infested tomato. The compost was applied a week before planting as soil amendment at the amount of 0.0, 0.5, 1.5, and 2.0 t·ha−1, while carbofuran was applied at the rate of 3.0 kg·ha−1. Four weeks old tomato seedlings cultivar ‘Roma VF’, which is susceptible to RKN, was transplanted to already prepared soil. Results of the experiment showed that the compost, especially in the amount 2 t·ha−1 and carbofuran at 3 kg·ha−1, brought about significant reduction of the population of RKN in soil and roots, and a significant increase in the growth and yield of tomato. The result of the experiment showed that Siam compost can be used for the managing root-knot nematodes in tomato cultivation, as an environmentally safe factor.
Effect of Root-knot Nematode (Meloidogyne incognita) on the Nodulation of Some Varieties of Cowpea (Vigna unguiculata L. Walp) N.B. Izuogu, T.U. Olajide, E.K. Eifediyi, C.M. Olajide Scientia Agriculturae Bohemica, 2019 Screenhouse and field trials were conducted at the University of Ilorin, Nigeria in 2013 and 2014 to investigate the effect of root knot nematode (Meloidogyne incognita) on the nodulation of five varieties of cowpea (IT89KD-288, IT82D-994, IT93K-452-1, T89K-391 and TT97 568-18). Half of the experimental plants were inoculated with Meloidogyne incognita eggs (n = 1500) while the remaining half served as uninoculated control plants. Data on growth and yield of cowpea, root gall, nodulation, and nematodes population densities in nodules were collected. Other parameters assessed included colour of dissected infected and uninfected nodules, texture of the nodules and varietal performance of the cowpeas. The results of both screenhouse and field trials were consistent in yield and visual observations of texture and colour. The yield of nematode-free varieties was significantly higher than that of M. incognita-infected ones. Infected nodules were dark brown in colour while the healthy ones were pink. For the field trials, nodulation counts in nematode inoculated plants were inversely proportional to the number of galls in their uninoculated counterparts in some varieties. Of all varieties, IT89KD-288 was most resistant to M. incognita. Due to high cost involved in the management of nematodes, especially with synthetic chemicals, variety IT89KD-288 is recommended for use in nematode endemic areas in Nigeria. Further study needs to be done on the histopathology of the infected and uninfected roots and nodules of these cowpea varieties.
Comparative evaluation of powdered and aqueous extract of citrus peels and carbofuran in the management of root-knot nematode on infected cowpea field Agriculturae Conspectus Scientificus, 2019
Control of root-knot nematode pest of okra using ocimum gratissimum compost Indian Journal of Nematology, 2018
The effectiveness of dry leaf powder of phyllanthus amarus scumach and thonn. and alstonia boonei de wild, on the root knot nematode, meloidogyne incognita attacking tomato solanum lycopersicum L. var. roma VF Tropical Agriculture, 2018
Control of nematode disease of eggplant (Solanum aethiopicum L.) using manure Agriculturae Conspectus Scientificus, 2013
RECENT SCHOLAR PUBLICATIONS
INTERNATIONAL JOURNAL OF PHYTOFUELS AND ALLIED SCIENCES HS Baba, NB Izuogu, A Anifowoshe, H Bello, J Asaramoge, OA Apalowo, ... INTERNATIONAL JOURNAL 14 , 2025 2025
Occurrence of virus in some watermelon growing areas of Kwara State, Nigeria OA Apalowo, O Balogun, NB Izuogu, A Banji, HS Baba, MJ Okafor, ... Selcuk Journal of Agriculture and Food Sciences 39 (2), 259-267 , 2025 2025
HISTOPATHOLOGY OF WATERMELON ROOT INFECTED WITH ROOT-KNOT NEMATODE (Meloidogyne spp.) IN KWARA STATE, NIGERIA OA Apalowo, NB Izuogu, HS Baba, CM Olajide, OS Balogun, ... FUDMA JOURNAL OF SCIENCES 9 (5), 114-117 , 2025 2025
Investigation of some selected botanicals as potential nematicides in the management of Meloidogyne incognita HS Baba, NB Izuogu, OF Folorunsho, OA Apalowo, CM Olajide, ... Published by the Nigeria Society of Nematologists (NISON , 2025 2025
Efficacy of Poultry Manure on Meloidogyne incognita Infection of Beetroot crop HS Baba, NB Izuogu, A Anifowoshe, H Bello, J Asaramoge, OA Apalowo, ... International Journal of Phytofuels and Allied Sciences, , 2025 2025
Penerapan Media Pembelajaran Quizziz Dalam Meningkatkan Hasil Belajar Matematika Kelas IV B di SDN Duren III Klari Karawang R Maulana, A Darmiyanti, A Rukajat Consilium: Education and Counseling Journal 4 (2), 517-526 , 2024 2024 Citations: 1
CHEMICAL CHARACTERIZATION OF Hyptis suaveolens AND Bridelia ferruginea AND THEIR ASSESSMENT FOR NEMATICIDAL PROPERTIES AGAINST Meloidogyne incognita INFECTING SOYBEAN (Glycine max) NB Izuogu, AA Anifowose, AB Adigun The International Journal of Organic Agriculture Research and Development 19 … , 2024 2024
POPULATION DISTRIBUTION OF PLANT-PARASITIC NEMATODES ASSOCIATED WITH JATROPHA AND TEAK PLANTATIONS IN THE UNIVERSITY OF ILORIN, NIGERIA NB Izuogu, CM Olajide, RA Apalowo, R Ocheze, AS Hinmikaiye Journal of Arid Agriculture 25 (2), 103-109 , 2024 2024
Efficacy of aqueous and powdered leaf extracts of Chromolaena odorata (Asterales: Asteraceae) and Ficus mucuso (Rosales: Moraceae) botanicals on root-knot nematode infecting … OA Apalowo, NB Izuogu, HS Baba, IO Adepoju, CM Olajide, MA Adeyemi, ... Iasi University of Life Sciences (IULS), Iași , 2024 2024 Citations: 1
Occurrence of parasitic nematodes infecting cucumber in Kwara State HS Baba, NB Izuogu, KY Belewu, AO Apalowo Published by Nnamdi Azikwe University , 2024 2024
Assessment of single and mixed infections of Meloidogyne enterolobii. OA Apalowo, NB Izuogu, HS Baba, CM Olajide, OS Balogun, ... Published by the Nigeria Society of Nematologists (NISON) , 2024 2024
Use of botanicals and poultry manure as potential bio-nematicides against root-knot nematodes infecting cucumber HS Baba, NB Izuogu, SY Yusuf, OA Apalowo, CM Olajide, IO Adepoju Bulletin of the Institute of Tropical Agriculture, Kyushu University 47, 11-31 , 2024 2024
Susceptibility of sweet potato varieties to Meloidogyne incognita and use of effective microorganisms and compost manure for the disease management AA Anifowose, NB Izuogu, BK Sossou Pakistan Journal of Nematology 41 (2), 135-143 , 2023 2023 Citations: 1
Nematodes Associated with Citrullus lanatus in Kwara State, Nigeria OA Apalowo, NB Izuogu, OS Balogun e-Proceedings of the Faculty of Agriculture International Conference, 401-407 , 2023 2023
Pathogenicity of root-knot nematode in cucumber and invitro test of poultry manure and botanical extracts as bionematicides H Baba, NB Izuogu Faculty of Agriculture, University of Abuja , 2023 2023
Controlling root-knot nematode Meloidogyne incognita in tomatoes using modified effective microorganisms-fermented plant extract and compost manure BK Sossou, NB Izuogu, AO Anifowose, HE Ahamefule International Journal of Recycling of Organic Waste in Agriculture 11 (4) , 2022 2022 Citations: 7
Genetic structure of cucumber mosaic virus from natural hosts in Nigeria reveals high diversity and occurrence of putative novel recombinant strains OA Apalowo, AO Adediji, OS Balogun, TI Fakolujo, JM Archibong, ... Frontiers in Microbiology 13, 753054 , 2022 2022 Citations: 19
Application of different doses of compost as a substitution of the commercial substrate in nursery for pepper and tomato seedlings ML Fiasconaro, MG Abrile, L Hintermeister, MC Antolín Bellver, ... Islamic Azad University , 2022 2022 Citations: 7
Plant parasitic nematodes associated with selected agricultural soils in Kwara State, Nigeria O Ahmed, NB Izuogu, HS Baba, NA Kalejaiye Agrosearch 20 (2), 56-66 , 2021 2021 Citations: 2
COMPARATIVE PROFITABILITY OF MANAGING MELOIDOGYNE INCOGNITA ON THE VEGETATIVE GROWTH OF CUCUMBERS (CUCUMIS SATIVUS L.) USING ROOT GROW (MYCORRHIZAL FUNGI) AND BROILER DROPPINGS OE Bello, NB Izuogu, OM Bello FUDMA JOURNAL OF SCIENCES 5 (2), 596-620 , 2021 2021
MOST CITED SCHOLAR PUBLICATIONS
Effects of organic and inorganic manures on the growth attributes of root-knot nematode (Meloidogyne incognita) infected Ethopian egg plant (Solanum aethiopicum) SA Abolusoro, PF Abolusoro, FO Mathew, NB Izuogu World Journal of Agricultural Research 1 (6), 104-107 , 2013 2013 Citations: 36
Efficacy of Trichoderma harzianumT22 as a biocontrol agent against root-knot nematode (Meloidogyne incognita) on some soybean varieties NB Izuogu, TO Abiri Croatian journal of food science and technology 7 (2), 47-51 , 2015 2015 Citations: 27
Control of nematode disease of egg plant ( Solanum aethiopicum ) using manure SA Abolusoro, MO Abe, PF Abolusoro, NB Izuogu Archives of Phytopathology and Plant Protection 48 (2), 188-193 , 2015 2015 Citations: 21
Genetic structure of cucumber mosaic virus from natural hosts in Nigeria reveals high diversity and occurrence of putative novel recombinant strains OA Apalowo, AO Adediji, OS Balogun, TI Fakolujo, JM Archibong, ... Frontiers in Microbiology 13, 753054 , 2022 2022 Citations: 19
A review on Borreria verticillata: A potential bionematicide, channeling its significant antimicrobial activity against root-knot nematodes NB Izuogu, OE Bello, OM Bello Heliyon 6 (10) , 2020 2020 Citations: 15
Toxicity of Aqueous and Powdered Sparrow grass, Asparagus africanus to Meloidogyne incognita on egg plant. NB Izuogu, O Oyedunmade, AM Usman, EEA EEA The International Journal of Organic Agriculture Research and Development 5 … , 2010 2010 Citations: 13
Control of root-knot nematode (Meloidogyne incognita) in tomato (Solanum lycopersicum) crop using siam weed (Chromolaena odorata) compost manure SA Abolusoro, AO Adekiya, C Aremu, S Ige, NB Izuogu, PF Abolusoro, ... Journal of Horticultural Research 28 (1), 87-92 , 2020 2020 Citations: 12
Effect of root-knot nematode ( Meloidogyne incognita ) on the nodulation of some varieties of cowpea ( Vigna unguiculata L. Walp). NB Izuogu, TU Olajide, EK Eifediyi, CM Olajide 2019 Citations: 12
Efficacy of neem compost on root knot nematode pest of Lagos spinach, Celosia argentea TI Olabiyi, JJ Atungwu, B Izuogu, J Akintola, S Abolusoro Archives of Phytopathology and Plant Protection 46 (18), 2253-2258 , 2013 2013 Citations: 12
Comparative evaluation of powdered and aqueous extract of citrus peels and carbofuran in the management of root-knot nematode on infected cowpea field NB Izuogu, C Azubuike, AS Hinmikaiye, A Olarenwaju Agriculturae Conspectus Scientificus 84 (2), 173-178 , 2019 2019 Citations: 11
Screenhouse Assessment of reaction of Fluted Pumpkin, Telfairia occidentalis Hook F. to Root-Knot Nematode Meloidogyne incognita NB Izuogu, EEA Oyedunmade, JO Babatola Journal of Agricultural Science , 2010 2010 Citations: 11
Nematicidal potential of aqueous extract of Hyptis suaveolens in the management of root-knot nematode, Meloidogyne incognita of some cowpea cultivars NB Izuogu, SA Abolusoro, LB Yakub Croatian journal of food science and technology 8 (1), 15-19 , 2016 2016 Citations: 9
Efficacy of aqueous leaf extracts of Negro coffee (Cassia occidentalis) and lemon grass (Cymbopogon citratus) in the management of nematode pests of okra (Abelmoschus … NB Izuogu, LB Yakubu, SA Abolusoro, W Nwabia Science, Technology and Arts Research Journal Sci. Technol. Arts Res. J. , 2015 2015 Citations: 8
The potency of Moringa oleifera and Jatropha curcas leaf extracts as control for root-knot nematode in maize (Zea mays) NB Izuogu, AA Badmos, SO Raji International Journal of Phytofuels and Allied Sciences , 2013 2013 Citations: 8
Comparative evaluation of some botanicals and carbofuran in the control of root-knot nematode, Meloidogyne spp. on cowpea (Vigna unguiculata) LB Yakubu, NB Izuogu Journal of Agriculture and Veterinary Sciences , 2013 2013 Citations: 8
Efficacy of aqueous extract of lemon grass (Andropogon citratus L.) against Root-Knot Nematode Pests of Okra.(Abelmoschus esculentus (L.) Moench) EEA Oyedunmade, NB Izuogu Agrosearch 11 (1), 31-38 , 2011 2011 Citations: 8
Controlling root-knot nematode Meloidogyne incognita in tomatoes using modified effective microorganisms-fermented plant extract and compost manure BK Sossou, NB Izuogu, AO Anifowose, HE Ahamefule International Journal of Recycling of Organic Waste in Agriculture 11 (4) , 2022 2022 Citations: 7
Application of different doses of compost as a substitution of the commercial substrate in nursery for pepper and tomato seedlings ML Fiasconaro, MG Abrile, L Hintermeister, MC Antolín Bellver, ... Islamic Azad University , 2022 2022 Citations: 7
Field evaluation of Trichoderma harzianum T22 for the management of Meloidogyne incognita (kofoid and white) Chitwood on cowpea varieties NB Izuogu, OV Osuwa, AO Fabiyi, TI Olabiyi, SA Abolusoro International Journal of Agriculture Science and Natural Resources , 2014 2014 Citations: 7
Control of nematode pest of okra Abelmoschus esculentus using two plant material and carbofuran NB Izuogu, EEA Oyedunmade, TI Olabiyi, II Oluwatayo, SA Abolusoro Nigerian Journal of Horticultural Science 16, 33-40 , 2011 2011 Citations: 7
