Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens Amira M. Helal, Osama Mahana, Ali Zanaty, Karim Selim, Mohamed. E. Taha, et al. Beni Suef University Journal of Basic and Applied Sciences, 2025 Background Avian pox viruses (APVs) are highly contagious poultry diseases, posing a major economic threat to poultry production systems, and have a high mortality rate among young birds, Infected birds also face condemnation of affected carcasses due to the unsightly appearance of the nodular skin lesions. This study aimed to provide an overview of the current genetic status of APV in backyard poultry, with a focus on the commercially available vaccines. To achieve this, molecular characterization and phylogenetic analysis of APVs were conducted, comparing their sequences with vaccine strains used in Egypt. Results The polymerase chain reaction (PCR) assay was able to detect APV in all the tested samples; 12 positive samples (6 chicken flocks and 6 pigeon flocks) were selected for DNA sequencing. The sequences were submitted to GenBank with accession numbers OR027032 to OR027043. The chicken strains exhibited 100% nucleotide identity with commercially available fowl pox virus (FPV) vaccines and were phylogenetically clustered within subclade A1 with other FPVs. On the other hand, the pigeon pox virus (PPV) strains were closely related to other PPV strains within subclade A2 and showing 100% and 91% nucleotide identity with the PPV and the FPV vaccines, respectively. Conclusions Despite the availability of APV vaccines in Egypt, a persistent threat of APV to poultry in the backyard system remains a significant concern. Our molecular characterization revealed the high genetic similarity between our field strains and commercially available vaccine strains, suggesting an urgent need for vaccination in backyard systems to counteract this emerging threat to bird populations.
Analysis of the hemagglutinin polypeptide of highly pathogenic avian influenza H5 clade 2.3.4.4b viruses isolated from backyard ducks and commercial chickens in Egypt Journal of Advanced Veterinary Research, 2025
Comparative analysis of commercial recombinant vaccination strategies against Newcastle disease in broilers German Journal of Veterinary Research, 2025 The widespread Newcastle disease (ND) genotype VII among vaccinated and unvaccinated chicken populations makes it one of the most threatening viruses impacting poultry, causing significant losses in the poultry industry. Recently, recombinant vector vaccine technology has proven to induce a durable protective immune response, even in the presence of maternal antibodies, thereby addressing the limitations associated with traditional vaccines. This study evaluated the protective efficacy of the recombinant turkey herpesvirus double construct vaccine (HVP360) that encodes both the NDV-F and IBDV VP2 genes alongside the recombinant fowl pox-based NDV vaccine (vFP96.5) and their combined use against virulent NDV genotype VII. For this purpose, 125 one-day-old broiler chicks (Cobb) were divided into five groups (G1-G5), in which chickens kept in G1 were unvaccinated unchallenged. Chickens kept in G2, G3, and G4 were vaccinated subcutaneously at one day old with HVP360, vFP96, and HVP360+vFP96, respectively. At 28 days of age, half of the birds (n=15) in each vaccinated group (G2, G3, and G4) and the G5 (positive control group) were challenged intraocularly with 106 EID50 velogenic NDV genotype VII. Protection was assessed based on mortalities, clinical signs, seroconversion, and viral shedding. Results demonstrated significant protection offered by the recombinant vaccines, reaching a remarkable level of 93.33% for G2 and G4, followed by 71.42% for G3, associated with a notable reduction of clinical signs and lesions. In contrast, the mortality rate reached 75% in unvaccinated challenged G5. Significant differences in seroconversion based on the hemagglutination (HI) test and ELISA were observed among vaccinated groups on days 14, 21, and 35, in which chickens kept in G3 exhibited the highest antibody titers, followed by G4 and G2. Viral shedding was significantly reduced in all vaccination groups at 3, 5, and 7 days post-challenge, with the highest reduction in dual-vaccinated chickens (G4) followed by G2. In conclusion, the concurrent application of HVP360 and vFP96.5 in combating the ND virus can establish a foundational basis for the vaccination program aimed at one-day-old chicks.
Herbal Oils and Probiotic Efficacy in Rabbits Challenged with Multidrug-Resistant Escherichia coli Journal of Advanced Veterinary Research, 2023
Biochemical, Histological and Ultrastructural Studies on the Effect of Citric acid Supplementation on Aflatoxins-intoxicated Japanese Quail Journal of Advanced Veterinary Research, 2023
Therapeutic Management, Clinicopathological, Molecular and Cost Studies on Sarcoptes scabiei Infestation in Rabbit Journal of Advanced Veterinary Research, 2023
The Occurrence of Low Pathogenic Avian Influenza H9 Viruses iBroiler Farms Within Ismailia Province, Egypt Journal of Advanced Veterinary Research, 2022
Current Genomic Characterization of Circulating Chicken Infectious Anemia Virus in Backyard and Commercial Chicken Flocks in Ismailia and Sharkia Provinces, Egypt Journal of Advanced Veterinary Research, 2022
Emergence of multidrug-resistant and virulent Escherichia coli with APEC‑associated traits in broiler chickens from Ismailia, Egypt RM ELTarabili, ME Abo Hashem, MA Ahmed, FM Yousseff, MS Abdallah, ... Scientific Reports 16 (1), 12067 , 2026 2026
Molecular Characterization and Genetic Evolution of Avian Reticuloendotheliosis Virus Genes Integrated in Different Avipoxviruses Genomes EAEM Shosha, AS Khalifa, W Mady, A Helal, MS Abdallah, D Arfa, ... Egyptian Journal of Veterinary Sciences, 1-21 , 2026 2026
Anti-inflammatory, Antioxidant, and Immunomodulatory Effect of Chlorella Vulgaris in Chicken Experimentally Infected with Eimeria tenella and Clostridia Perfringens NB Elbarbary, A El-Wahab, A Reham, WM Gad, S Zayed, WS Darwish, ... Egyptian Journal of Veterinary Sciences 56 (13), 211-223 , 2025 2025 Citations: 1
Molecular Detection of Respiratory Virus Infection and Co-infection in Chicken Farms ASA Mohamed, E Dimerdash, M Zaky, WK Elfeil, MS Abdallah Egyptian Journal of Veterinary Sciences 56 (13), 165-174 , 2025 2025
Antibacterial effects of nano-emulsified cumin oil on performance and carcass characteristics in weaning rabbits infected by Clostridium perfringens type A RR Fathy, M Abaza, Z Mohamed, AH Tantawy, M Abdallah, NM khalaf, ... Beni-Suef University Journal of Basic and Applied Sciences 14 (1), 73 , 2025 2025 Citations: 2
Tracing the immune response of different formulations of necrotic enteritis vaccine adjuvanted with cumin oil nano emulsion in chickens. RF Reda, M Abaza, M Abdallah, N Khalaf, M Ahmed, AA El-Bakey 2025
Evaluation of Biosecurity Measures and Microbiological Quality of Table Eggs in Egyptian Layer Farms E Hafez, E Nafei, M Abdallah, EA El Akshar, AN Elkattan, M Elkhayat, ... Veterinaria Italiana 61 (2) , 2025 2025
Tracing the immune response of different formulations of necrotic enteritis vaccine adjuvanted with cumin oil Nano emulsion in chickens MA Abaza, RR Fathy, M Abdallah, NM Khalaf, MM Ahmed, ... Benha Veterinary Medical Journal 48 (1), 13-18 , 2025 2025 Citations: 1
Analysis of the hemagglutinin polypeptide of highly pathogenic avian influenza H5 clade 2.3. 4.4 b viruses isolated from backyard ducks and commercial chickens in Egypt MMS Shelkamy, DM Hamed, MA Soltan, MS Abdallah, HMF Abdien Journal of Advanced Veterinary Research 15 (2), 248-254 , 2025 2025
Identification and genetic analysis of the recent Avian pox viruses from infected pigeons and chickens AM Helal, O Mahana, A Zanaty, K Selim, ME Taha, MM Saleh, MI Hassan, ... Beni-Suef University Journal of Basic and Applied Sciences 14 (1), 11 , 2025 2025 Citations: 1
Immunogenicity of a novel inactivated trivalent avian reovirus vaccine in Egyptian broiler breeders and their progeny E Arafa, HMF Abdien, WK Elfeil, MAZ El-Abideen, MS Abdallah Adv. Anim. Vet. Sci 13 (s1), 189-197 , 2025 2025
The Formal Image of Poverty and the Poor People in the Design of the State Advertisement to Combat Poverty in Egypt M Abdallah Journal of Design Sciences and Applied Arts 6 (1), 188-198 , 2025 2025
Comparative analysis of commercial recombinant vaccination strategies against Newcastle disease in broilers HMF Abdien, MMZ El-Dimerdash, EA Morsy, NM Ali, MS Abdallah Ger. J. Vet. Res 5 (1), 17-29 , 2025 2025
International Journal of Veterinary Science H El Daous, NA El Din, E Nafei, M Abdallah, AM Abd-El Hamed, ... Int J Vet Sci 14 (1), 79-89 , 2025 2025
Incidence and virulence gene profiling of Pseudomonas aeruginosa in broiler chickens from Fayoum Governorate, Egypt AI Morsi, M Abdallah, A Rafequ, M Elkhayat Benha Veterinary Medical Journal 47 (2), 78-81 , 2024 2024 Citations: 1
Determination of pathogenic E. coli and antimicrobial resistance genes in dogs and human semen: evidence of multidrug-resistance and antimicrobial resistance gene profiles RH Taalab, RH Hamouda, MS Abdallah, ME Elkhayat Benha Veterinary Medical Journal 47 (1), 135-142 , 2024 2024 Citations: 3
Subclinical carotid atherosclerosis in gouty patients: relation to musculoskeletal ultrasonographic findings and serum sclerostin level. M Mohamed, M Abdallah, H Fawzy, R Ali Romanian Journal of Rheumatology/Revista Romana de Reumatologie 33 (3) , 2024 2024
The effectiveness of protective measures against Streptococcosis and the immune responses triggered by the administration of live, live-attenuated, and killed vaccines were … A El-daim, AF Matter, MG Mohamed, M Abdallah, WS Raslan, HA Youssef Journal of Advanced Veterinary Research 14 (2), 316-321 , 2024 2024 Citations: 2
Outcome of cataract surgery in children with presumed trematode-induced granulomatous anterior uveitis M Abdallah, AK Al-Hussaini, W Soliman, MGA Saleh BMC ophthalmology 24 (1), 21 , 2024 2024 Citations: 7
Comparative prevalence of virulence genes and antibiotic resistance in Campylobacter jejuni isolated from broilers, laying hens and farmers A Shami, M Abdallah, MW Alruways, YS Mostafa, SA Alamri, AE Ahmed, ... Pak. Vet. J 44 (1), 200-204 , 2024 2024 Citations: 21
MOST CITED SCHOLAR PUBLICATIONS
Avian Paramyxovirus Type 1 in Egypt: Epidemiology, Evolutionary Perspective, and Vaccine Approach SMG Mansour, RM ElBakrey, FF Mohamed, EE Hamouda, MS Abdallah, ... Frontiers in veterinary science 8, 647462 , 2021 2021 Citations: 42
Assessment of biosecurity measures in broiler’s farms in the Suez Canal area–Egypt using a seasonal prevalence of Salmonellosis ES Soliman, MS Abdallah Veterinary World 13 (4), 622 , 2020 2020 Citations: 23
Comparative prevalence of virulence genes and antibiotic resistance in Campylobacter jejuni isolated from broilers, laying hens and farmers A Shami, M Abdallah, MW Alruways, YS Mostafa, SA Alamri, AE Ahmed, ... Pak. Vet. J 44 (1), 200-204 , 2024 2024 Citations: 21
Preventive antimicrobial action and tissue architecture ameliorations of Bacillus subtilis in challenged broilers ES Soliman, RT Hamad, MS Abdallah Veterinary World 14 (2), 523 , 2021 2021 Citations: 9
The occurrence of low pathogenic avian influenza H9 viruses in broiler farms within Ismailia Province, Egypt MMS Shelkamy, HMF Abdien, DM Hamed, MA Soltan, MS Abdallah Journal of Advanced Veterinary Research 12 (4), 346-352 , 2022 2022 Citations: 8
Outcome of cataract surgery in children with presumed trematode-induced granulomatous anterior uveitis M Abdallah, AK Al-Hussaini, W Soliman, MGA Saleh BMC ophthalmology 24 (1), 21 , 2024 2024 Citations: 7
Herbal oils and probiotic efficacy in rabbits challenged with multidrug-resistant Escherichia coli MS Abdallah, HM Hassan, WAA Ibrahim, AM Helal, EA Hamed Journal of Advanced Veterinary Research 13 (8), 1491-1496 , 2023 2023 Citations: 7
Detection of some virulence and antibiotic resistance genes in Campylobacter jejuni isolated from poultry and human M Abdallah, MA Abaza, RR Fathy, AG Youseef, M Sobhy, ... The Egyptian Journal of Hospital Medicine 89 (2), 6373-6381 , 2022 2022 Citations: 7
Enhancing spearmint productivity and water use efficiency under alternative planting practices A Amer, M Abdallah, T Noreldin Journal of Central European Agriculture 20 (3), 852-865 , 2019 2019 Citations: 7
Growth and productivity of wheat as affected by putrescine and spermidine under water stress conditions. MA Ahmed, MAF Shalaby, MSA Abdallah 2012 Citations: 7
Alleviation of water stress in coriander plants by foliar application of boron M Abdallah, A El-Khalek, N Sarah, EF AbuEl-leil Scientific Journal of Flowers and Ornamental Plants 9 (4), 307-317 , 2022 2022 Citations: 6
Therapeutic management, clinicopathological, molecular and cost studies on Sarcoptes scabiei infestation in rabbit MS Abdallah, NH Sallam, NS Abdelnaeim, MA Mandour, WFS Eldin, ... Journal of Advanced Veterinary Research 13 (3), 333-338 , 2023 2023 Citations: 5
Allogeneic-bloodsparing effect of tranexamic acid versus acute normovolemic hemodilution for total hip replacement AA Ahmed, H Abdelrhman, M Zaky, M Abdallah Egyp J Anaesth 26, 23-30 , 2010 2010 Citations: 5
Protective efficacy of inactivated H9N2 vaccine in Turkey poults under both experimental and field conditions WK Elfeil, H Youssef, A Sedeek, A El-Shemy, EM Abd-Allah, MF Elkady, ... Vaccines 10 (12), 2178 , 2022 2022 Citations: 4
Nitrogen fertilizer effect on basil and water productivity A Amer, M Abdallah, T Noreldin, MF Mohamed J Soils Crops 29 (1), 23-31 , 2019 2019 Citations: 4
Protective efficacy of synbiotics in the prevention of Salmonella Typhimurium in chickens M El-Demerdash, H Abdien, D Mansour, W Elfeil, MS Abdallah Glob. Anim. Sci. J 2 (2), 78-85 , 2015 2015 Citations: 4
Determination of pathogenic E. coli and antimicrobial resistance genes in dogs and human semen: evidence of multidrug-resistance and antimicrobial resistance gene profiles RH Taalab, RH Hamouda, MS Abdallah, ME Elkhayat Benha Veterinary Medical Journal 47 (1), 135-142 , 2024 2024 Citations: 3
Brief report first report of the in vitro ovicidal activity of camel milk and its fractions on zoonotic-liver fluke (Fasciola gigantica) eggs DAB Awad, AS Eldiarby, M Abdallah, A Hamad, SMA Gawad Veterinary Research Communications 47 (4), 2293-2300 , 2023 2023 Citations: 3
Current Genomic Characterization of Circulating Chicken Infectious Anemia Virus in Backyard and Commercial Chicken Flocks in Ismailia and Sharkia Provinces, Egypt HMF Abdien, DM Hamed, WK Elfeil, AA Selim, DSA Elhalous, ... Journal of Advanced Veterinary Research 12 (5), 513-519 , 2022 2022 Citations: 3
Preventive antimicrobial action and tissue architecture ameliorations of Bacillus subtilis in challenged broilers. Veterinary World, 14 (2): 523-536 ES Soliman, RT Hamad, MS Abdallah 2021 Citations: 3