Development and Assessment of Copper-Based Nanoparticles Derived from Dypsis lutescens for Their Antibacterial Properties by in vitro Application Abdul Rahamanulla, Syed Suhaib Ahmed, Gulzar Ahmed Mohammed, Rokeya Sultana, Inamul Hasan Madar, Ayesha Sultana, Hanin Khadija, Abdulla Ruknuddin Journal of Health Science and Medical Research, 2026 Objective: Phytoconstituents, including polyphenols, tannins, alkaloidsandflavonoids, which are abundant in Dypsis lutescens leaf extract, were utilized for the green synthesis of copper nanoparticles (g-CuNPs). These bioactive compounds served as natural reducing agentsandcapping agents in the synthesis process.Material and Methods: The formation of g-CuNPs was confirmed by ultraviolet spectroscopy (UV), showing a characteristic λmax at 410 nm. Functional groups of the capping agents on g-CuNPs were verified using Fourier transform infrared spectroscopy (FTIR spectroscopy).Results: The nanoparticles demonstrated remarkable stability, as confirmed by Malvern Zetasizer analysis. They exhibited an acceptable particle size and Poly Dispersity Index (PDI), along with a robust positive zeta potential of +40, indicating their suitability for biological applications. Antibacterial assays revealed that the g-CuNPs significantly enhanced antibacterial activity compared to the leaf extract alone. The inhibition zones were slightly higher for both Gram-positive and Gram-negative bacteria, including Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Staphylococcus aureus (S. aureus) and Enterococcus faecalis (E. faecalis), underscoring the efficacy of the nanoparticles in combating bacterial pathogens.Conclusion: This study underscores the potential of integrating bioactive phytochemicals with copper nanoparticles (CuNPs) to create potent antibacterial agents. The enhanced activity of g-CuNPs against both Gram-positive and Gram-negative bacteria highlights their promise as effective solutions for addressing challenges posed by pathogenic bacteria. This innovative approach paves the way for developing advanced antimicrobial therapies with improved efficiency and stability.
An occupational therapy lens on microbiota–gut–brain modulation in ASD and ADHD Anas, Syed Suhaib Ahmed, Inamul Hasan Madar Frontiers in Rehabilitation Sciences, 2026 Research on the microbiota gut brain axis has expanded rapidly, with growing interest in its potential relevance to neurodevelopmental conditions such as autism spectrum disorder and attention-deficit hyperactivity disorder. Systematic reviews increasingly report associations between gut microbial alterations and behavioral features in these populations, yet causal mechanisms remain unconfirmed and translational relevance for rehabilitation remains limited. Much of the existing literature prioritizes microbial composition, inflammatory markers, and neurochemical correlates, with comparatively little attention to everyday functioning, participation, and lived experience. This Perspective argues that microbiota gut brain interactions should be conceptualized within rehabilitation sciences as contextual physiological modulators, referring to internal bodily processes that shape readiness and engagement without determining occupational performance outcomes, and influencing occupational readiness and regulatory capacity rather than acting as etiological drivers of participation restrictions. Drawing on ecological and occupation-centered models of function, including the International Classification of Functioning framework and occupational therapy theories of performance and participation, we propose a reframing that preserves functional orientation while integrating emerging neurophysiological insights. The paper outlines implications for rehabilitation assessment, intervention planning, and interdisciplinary collaboration, and advances programmatic directions for future research that prioritize participation-based outcomes and ecological validity, defined here as the extent to which outcomes reflect real-world participation and everyday functioning rather than laboratory-based or symptom-only change. Repositioning microbiota gut brain research within a participation-focused rehabilitation framework may enhance translational value and support function-oriented, interdisciplinary care for children with autism spectrum disorder and attention-deficit hyperactivity disorder.
Dissecting the Phospho-Regulatory Landscape of Protein Kinase N1 (PKN1) and Its Downstream Signaling: Functional Insights into the Activity-Dependent and Disease-Relevant Phosphosites Sreeshma Ravindran Kammarambath, Leona Dcunha, Athira Perunelly Gopalakrishnan, Yashi Shailendra Gautam, Furqaan Ahmed Basha, Prathik Basthikoppa Shivamurthy, Inamul Hasan Madar, Rajesh Raju International Journal of Molecular Sciences, 2026 Protein Kinase N1 (PKN1) is a PKC-related serine/threonine kinase of the AGC group within the eukaryotic protein kinase superfamily (ePK) that orchestrates oncogenic, metabolic, and cytoskeletal signaling. Despite these critical roles, the phosphorylation-dependent regulatory network of PKN1 remains largely undefined. We performed a large-scale phosphoproteomic data integration of publicly available human datasets (892 profiling datasets and 191 differential datasets) to identify recurrent PKN1 phosphorylation sites. This analysis identified two predominant PKN1 phosphosites, S562 and S916, that were frequently observed and differentially regulated across studies. The S916 maps to a turn motif (TM) in the AGC group of kinases, which is evolutionarily conserved among PKN paralogs, while S562 is non-conserved and appears to be PKN1-specific. Co-regulation and enrichment analyses suggest that S916 is associated with insulin/AMPK signaling and metabolic pathways, whereas S562 co-occurs with phosphosites involved in cell division, cytoskeletal regulation, and microtubule cytoskeleton organization. Integrating predicted and experimentally validated kinases, substrates, and interactors, we reconstructed a phospho-regulatory network that positions PKN1 at the crossroads of cytoskeleton organization and metabolic signaling. To assess the disease relevance of these phosphorylation events, we integrated transcriptomic and phosphoproteomic data from the hepatocellular carcinoma database (HCCDB). PKN1 was markedly up-regulated in HCC, and its phosphorylation at S916 was positively co-regulated with multiple oncogenic and proliferation-associated protein phosphosites. These results predict S562 and S916 as potential sites for targeted biochemical validation and functional experiments. The identification of S562 and S916 as key regulatory sites provides new mechanistic insight into PKN1 activation and highlights potential avenues for therapeutic targeting.
Proteogenomic characterization of molecular and cellular targets for treatment-resistant subtypes in locally advanced cervical cancers Do Young Hyeon, Dowoon Nam, Hye-Jin Shin, Juhee Jeong, Eunsoo Jung, Soo Young Cho, Dong Hoon Shin, Ja-Lok Ku, Hye Jung Baek, Chong Woo Yoo, Eun-Kyung Hong, Myong Cheol Lim, Sang-Jin Lee, Young-Ki Bae, Jong Kwang Kim, Jingi Bae, Wonyoung Choi, Su-Jin Kim, Seunghoon Back, Chaewon Kang, Inamul Hasan Madar, Hokeun Kim, Suhwan Kim, Duk Ki Kim, Jihyung Kang, Geon Woo Park, Ki Seok Park, Yourae Shin, Sang Soo Kim, Keehoon Jung, Daehee Hwang, Sang-Won Lee, Joo-Young Kim Molecular Cancer, 2025
Proteogenomic landscape of human pancreatic ductal adenocarcinoma in an Asian population reveals tumor cell-enriched and immune-rich subtypes Do Young Hyeon, Dowoon Nam, Youngmin Han, Duk Ki Kim, Gibeom Kim, Daeun Kim, Jingi Bae, Seunghoon Back, Dong-Gi Mun, Inamul Hasan Madar, Hangyeore Lee, Su-Jin Kim, Hokeun Kim, Sangyeop Hyun, Chang Rok Kim, Seon Ah Choi, Yong Ryoul Kim, Juhee Jeong, Suwan Jeon, Yeon Woong Choo, Kyung Bun Lee, Wooil Kwon, Seunghyuk Choi, Taewan Goo, Taesung Park, Young-Ah Suh, Hongbeom Kim, Ja-Lok Ku, Min-Sik Kim, Eunok Paek, Daechan Park, Keehoon Jung, Sung Hee Baek, Jin-Young Jang, Daehee Hwang, Sang-Won Lee Nature Cancer, 2023
A review on the application of bioinformatics tools in food microbiome studies Ramachandran Chelliah, Eric Banan-MwineDaliri, Imran Khan, Shuai Wei, Fazle Elahi, Su-Jung Yeon, Vijayalakshmi Selvakumar, Fred Kwame Ofosu, Momna Rubab, Hum Hun Ju, Harikrishna Reddy Rallabandi, Inamul Hasan Madar, Ghazala Sultan, Deog Hwan Oh Briefings in Bioinformatics, 2022
Risk assessment and regulatory decision-making for nanomaterial use in agriculture Ramachandran Chelliah, Inamu lHasan Madar, Ghazala Sultan, Mahamuda Begum, Bandana Pahi, Iftikhar Aslam Tayubi, Vijayalakshmi Selvakumar, Deog Hwan Oh Engineered Nanomaterials for Sustainable Agricultural Production Soil Improvement and Stress Management, 2022
The role of bioactive peptides in diabetes and obesity Ramachandran Chelliah, Shuai Wei, Eric Banan-Mwine Daliri, Fazle Elahi, Su-Jung Yeon, Akanksha Tyagi, Shucheng Liu, Inamul Hasan Madar, Ghazala Sultan, Deog-Hwan Oh Foods, 2021
Unveiling the potentials of bacteriocin (Pediocin L50) from Pediococcus acidilactici with antagonist spectrum in a Caenorhabditis elegans model Ramachandran Chelliah, Kandasamy Saravanakumar, Eric Banan-Mwine Daliri, Joong-Hark Kim, Jung-Kun Lee, Hyeon-yeong Jo, Se-Hun Kim, Sudha Rani Ramakrishnan, Inamul Hasan Madar, Shuai Wei, Momna Rubab, Kaliyan Barathikannan, Fred Kwame Ofosu, Hwang Subin, Park Eun-ji, Jung Da Yeong, Fazle Elahi, Myeong-Hyeon Wang, Jong Hwan Park, Juhee Ahn, Dong-Hwan Kim, Sung Jin Park, Deog-Hwan Oh International Journal of Biological Macromolecules, 2020
Mycological quality of fresh and frozen chicken meat retailed within Warri metropolis, Delta State, Nigeria Jordan Journal of Biological Sciences, 2017
Polymorphism of angiotensin I converting enzyme becomes an innate property in renal failure International Journal of Pharma and Bio Sciences, 2010
RECENT SCHOLAR PUBLICATIONS
Phosphorylation-dependent regulation of Eleven Lysine Rich Leukemia (ELL) uncovered by quantitative global phosphoproteomic analysis SR Kammarambath, L Dcunha, A Fahma, S Subair, AP Gopalakrishnan, ... Molecular Genetics and Genomics 301 (1), 71 , 2026 2026
Formulation and Characterization of Rivastigmine Nano Lipid Mucoadhesive Gel Using Moringa oleifera Mucilage for Alzheimer’s Disease B Das, MS Umashankar, IH Madar Journal of Pharmaceutical Innovation 21 (4), 425 , 2026 2026
Past, present and future of nivolumab: new horizons in immuno-oncology IH Madar, SR Kammarambath Frontiers in Oncology 16, 1801874 , 2026 2026
An occupational therapy lens on microbiota–gut–brain modulation in ASD and ADHD Anas, SS Ahmed, IH Madar Frontiers in Rehabilitation Sciences 7, 1807954 , 2026 2026
Development and Assessment of Copper-Based Nanoparticles Derived from Dypsis lutescens for Their Antibacterial Properties by in vitro Application A Rahamanulla, SS Ahmed, GA Mohammed, R Sultana, IH Madar, ... Journal of Health Science and Medical Research 44 (3), 20251264 , 2026 2026
Dissecting the Phospho-Regulatory Landscape of Protein Kinase N1 (PKN1) and Its Downstream Signaling: Functional Insights into the Activity-Dependent and Disease-Relevant … SR Kammarambath, L Dcunha, AP Gopalakrishnan, YS Gautam, ... International Journal of Molecular Sciences 27 (5), 2137 , 2026 2026
Comment on “Leisure-time physical activity patterns and predictors in patients before and after metabolic and bariatric surgery: a cross-sectional study” K Roy, MM Ul Haq, IH Madar Obesity Surgery 36 (2), 912-913 , 2026 2026 Citations: 1
Shaping Immunotherapy Through the Tumor Microenvironment: Translational Perspectives V Vohra, M Dhanawat, R Chalotra, B Bhushan, J Girdhar, IH Madar, ... Critical Reviews in Oncology/Hematology, 105169 , 2026 2026 Citations: 2
Comprehensive Analysis of Gene Expression Profiles in Pancreatic Cancer: Insights into Biomarkers and Therapeutic Targets R Suresh, CA Selvarasu, IH Madar, R Majji, ME Santhosh, A Krishnan Journal of Clinical Medicine of Kazakhstan 23 (1), 41-51 , 2026 2026
Comment on “Incorporating Artificial Intelligence in Qualitative Research: Exploring the Role of ChatGPT in Thematic Analysis” M Basavaraj, S Banerjee, IH Madar Medical Science Educator, 1-2 , 2026 2026
Comment on "The Effect of Artificial Intelligence in Promoting Positive Nursing Practice Environments: Mixed Methods Systematic Review" LSR Inamul Hasan Madar, Sanjay L Kurkute Journal of Clinical Nursing (JCN) 19 , 2026 2026
HPV-driven transcriptome and splicing rewiring under SRPK1 inhibition in cervical cancer A Basera, M Alaouna, J Duvenhage, DO Bates, Z Dlamini, R Marima Frontiers in Oncology 15, 1712170 , 2026 2026
Ryszard Smolarczyk, Maria Sklodowska Curie Memorial Institute, Poland IH Madar, L Lu, M Balsa, J Martin-Liberal, M Balsa, F Torrent, D Pérez, ... 2026
Sensory-adapted dentistry: oral care for autistic children in dental environments Anas, SS Ahmed, KS Chinannai, IH Madar Irish Journal of Medical Science (1971-), 1-2 , 2025 2025
Correction: Proteogenomic characterization of molecular and cellular targets for treatment‑resistant subtypes in locally advanced cervical cancers DY Hyeon, D Nam, HJ Shin, J Jeong, E Jung, SY Cho, DH Shin, JL Ku, ... Molecular Cancer 24 (1), 174 , 2025 2025
Role of histone-lysine N-methyltransferase 2D (KMT2D) in MEK-ERK signaling-mediated epigenetic regulation: a phosphoproteomics perspective SR Kammarambath, L Dcunha, AP Gopalakrishnan, A Fahma, N Krishna, ... Frontiers in Bioinformatics 5, 1683469 , 2025 2025 Citations: 4
Cerium oxide nanoparticles synthesized via green route exhibit neuroprotective effects in Alzheimer’s-induced Albino Wistar rats P Sriramcharan, SS Ahmed, J Natarajan, RR Kumar, J Antony, AP Kumar, ... 3 Biotech 15 (11), 1-16 , 2025 2025
BYS10, a novel selective RET inhibitor, exhibits potent antitumor activity in preclinical models F Qin, Y Chen, J Deng, R Guo, Z Xie, Z Luo, L Wang, T Huang, J Zhao, ... Frontiers in Pharmacology 16, 1670140 , 2025 2025 Citations: 1
Strategies for the drug development of cancer therapeutics H Liu, Y Ma, W Chen, X Gu, J Sun, P Li Frontiers in Pharmacology 16, 1656012 , 2025 2025 Citations: 4
GNA13 promotes brain metastasis of non-small cell lung cancer and EMT through the WNT/β catenin signaling pathway JQ Wu, HP Zou, RY Fan, J Cai, P Wei, MF He, J Xiang Frontiers in Cell and Developmental Biology 13, 1652200 , 2025 2025 Citations: 1
MOST CITED SCHOLAR PUBLICATIONS
Advances in smart nanomaterials and their applications A Husen, KS Siddiqi Elsevier , 2023 2023 Citations: 166
The role of bioactive peptides in diabetes and obesity R Chelliah, S Wei, EBM Daliri, F Elahi, SJ Yeon, A Tyagi, S Liu, IH Madar, ... Foods 10 (9), 2220 , 2021 2021 Citations: 92
Concept of sample in OMICS technology N Morrison, G Cochrane, N Faruque, T Tatusova, Y Tateno, D Hancock, ... OMICS: A Journal of Integrative Biology 10 (2), 127-137 , 2006 2006 Citations: 63
Proteogenomic landscape of human pancreatic ductal adenocarcinoma in an Asian population reveals tumor cell-enriched and immune-rich subtypes DY Hyeon, D Nam, Y Han, DK Kim, G Kim, D Kim, J Bae, S Back, DG Mun, ... Nature cancer 4 (2), 290-307 , 2023 2023 Citations: 58
Towards the early detection of ductal carcinoma (a common type of breast cancer) using biomarkers linked to the PPAR (γ) signaling pathway G Sultan, S Zubair, IA Tayubi, HU Dahms, IH Madar Bioinformation 15 (11), 799 , 2019 2019 Citations: 52
Antioxidant activities of novel peptides from Limosilactobacillus reuteri fermented brown rice: A combined in vitro and in silico study A Tyagi, R Chelliah, EBM Daliri, G Sultan, IH Madar, NH Kim, U Shabbir, ... Food chemistry 404, 134747 , 2023 2023 Citations: 51
Comprehensive in silico analysis of lactic acid bacteria for the selection of desirable probiotics S Vijayalakshmi, DE Adeyemi, IY Choi, G Sultan, IH Madar, MK Park Lwt 130, 109617 , 2020 2020 Citations: 45
Epigenetic drug screening defines a PRMT5 inhibitor–sensitive pancreatic cancer subtype F Orben, K Lankes, C Schneeweis, Z Hassan, H Jakubowsky, L Krauß, ... JCI insight 7 (10), e151353 , 2022 2022 Citations: 35
A review on the application of bioinformatics tools in food microbiome studies R Chelliah, E Banan-MwineDaliri, I Khan, S Wei, F Elahi, SJ Yeon, ... Briefings in Bioinformatics 23 (2), bbac007 , 2022 2022 Citations: 34
Identification of marker genes in Alzheimer's disease using a machine-learning model IH Madar, G Sultan, IA Tayubi, AN Hasan, B Pahi, A Rai, PK Sivanandan, ... Bioinformation 17 (2), 348 , 2021 2021 Citations: 34
An in silico analytical study of lung cancer and smokers datasets from gene expression omnibus (GEO) for prediction of differentially expressed genes AN Hasan, MW Ahmad, IH Madar, BL Grace, TN Hasan Bioinformation 11 (5), 229 , 2015 2015 Citations: 32
Biostimulants in plant protection and performance A Husen Elsevier , 2024 2024 Citations: 26
Unveiling the potentials of bacteriocin (Pediocin L50) from Pediococcus acidilactici with antagonist spectrum in a Caenorhabditis elegans model R Chelliah, K Saravanakumar, EBM Daliri, JH Kim, JK Lee, H Jo, SH Kim, ... International journal of biological macromolecules 143, 555-572 , 2020 2020 Citations: 26
Measurements of heterotypic associations between cluster of differentiation CD74 and CD44 in human breast cancer-derived cells H Al Ssadh, PS Spencer, W Alabdulmenaim, R Alghamdi, IH Madar, ... Oncotarget 8 (54), 92143 , 2017 2017 Citations: 24
Intelligent packaging systems: food quality and intelligent medicine box based on nano-sensors R Chelliah, I Khan, S Wei, IH Madar, G Sultan, EBM Daliri, C Swamidoss, ... Smart nanomaterials in biomedical applications, 555-587 , 2022 2022 Citations: 20
Smart nanomaterials in biomedical applications JC Kim, M Alle, A Husen Springer International Publishing , 2021 2021 Citations: 19
Multiplexed Post-Experimental Monoisotopic Mass Refinement ( m PE-MMR) to Increase Sensitivity and Accuracy in Peptide Identifications from Tandem Mass … IH Madar, SI Ko, H Kim, DG Mun, S Kim, RD Smith, SW Lee Analytical chemistry 89 (2), 1244-1253 , 2017 2017 Citations: 19
Mycological Quality of Fresh and Frozen Chicken Meat Retailed within Warri Metropolis, Delta State, Nigeria. GI Ogu, IH Madar, JC Igborgbor, JC Okolo Jordan Journal of Biological Sciences 10 (4) , 2017 2017 Citations: 18
CRISPR/Cas9: Role of genome editing in cancer immunotherapy S Vimal, IH Madar, L Thirumani, L Thangavelu, AM Sivalingam Oral Oncology Reports 10, 100251 , 2024 2024 Citations: 17
Knockdown of CD-74 in the proliferative and apoptotic activity of breast cancer cells H Al Ssadh, W Al Abdulmonem, Z Rasheed, IH Madar, J Alhoderi, ... Open access Macedonian journal of medical sciences 7 (19), 3169 , 2019 2019 Citations: 17
