Cirurgiã-Dentista pela Faculdade de Odontologia da Universidade de São Paulo (2022). Foi bolsista FAPESP (2018-2020) e CNPq-PIBIC (2021-2022). Teve como linhas de pesquisa o campo da Engenharia de Tecido Ósseo com ênfase na Terapia por Fotobiomodulação, Reparo ósseo e cultura de Células-tronco Mesenquimais e Osteoblastos na área da Cirurgia e Traumatologia Bucomaxilofacial.
EDUCATION
Graduação em Odontologia - FOUSP
RESEARCH INTERESTS
Terapia celular, engenharia de tecidos, cultura de células
2
Scopus Publications
Scopus Publications
Photobiomodulation treatments drive osteogenic versus adipocytic fate of bone marrow mesenchymal stem cells reversing the effects of hyperglycemia in diabetes Natália Pieretti Bueno, Cecília Cardoso Kfouri, Isabella Nunes Copete, Fabíola Singaretti de Oliveira, Praveen Arany, et al. Lasers in Medical Science, 2022 Diabetes mellitus (DM) is a chronic metabolic disease that affects bone metabolism, which can be related to a reduced osteogenic potential of bone marrow mesenchymal stem cells (BM-MSCs). MSCs from diabetic rats (dBM-MSC) have shown a tendency to differentiate towards adipocytes (AD) instead of osteoblasts (OB). Since photobiomodulation (PBM) therapy is a non-invasive treatment capable of recovering the osteogenic potential of dBM-MSCs, we aimed to evaluate whether PBM can modulate MSC’s differentiation under hyperglycemic conditions. BM-MSCs of healthy and diabetic rats were isolated and differentiated into osteoblasts (OB and dOB) and adipocytes (AD and dAD). dOB and dAD were treated with PBM every 3 days (660 nm; 5 J/cm2; 0.14 J; 20 mW; 0.714 W/cm2) for 17 days. Cell morphology and viability were evaluated, and cell differentiation was confirmed by gene expression (RT-PCR) of bone (Runx2, Alp, and Opn) and adipocyte markers (Pparγ, C/Ebpα, and C/Ebpβ), production of extracellular mineralized matrix (Alizarin Red), and lipid accumulation (Oil Red). Despite no differences on cell morphology, the effect of DM on cells was confirmed by a decreased gene expression of bone markers and matrix production of dOB, and an increased expression of adipocyte and lipid accumulation of dAD, compared to heatlhy cells. On the other hand, PBM reversed the effects of dOB and dAD. The negative effect of DM on cells was confirmed, and PBM improved OB differentiation while decreasing AD differentiation, driving the fate of dBM-MSCs. These results may contribute to optimizing bone regeneration in diabetic patients.
