Bachelor's degree: Animal Science (UFRRJ, Rio de Janeiro, RJ)
M.Sc.: Animal Nutrition and Production (USP, Pirassununga, SP)
PhD: Animal Science and Pastures (USP, Piracicaba, SP)
Sabbatical: The University of Nottingham (UK)
Cactus Cladodes and Sugarcane Bagasse Can Partially Replace Earless Corn Silage in Diets of Lactating Dairy Cows Izaac P. S. Medeiros, Sebastião I. Guido, Marco A. S. Gama, Carlos H. M. Silva, Michelle C. B. Siqueira, et al. Dairy, 2024 This study aimed to evaluate the effects of replacing earless corn silage (ECS) with cactus cladodes (CC; Opuntia spp.) and sugarcane bagasse (SB) on nutrient intake, digestibility, feeding behavior, milk yield (MY), and composition of lactating dairy cows. Ten Holstein cows, weighing 571 ± 97.0 kg and producing 23.0 ± 4.4 kg of milk per day, were assigned to two contemporaneous 5 × 5 Latin squares. Treatments consisted of five levels of ECS replacement with CC plus SB (0, 25, 50, 75, and 100%). The results showed a linear increase in dry matter (DM) intake (p < 0.05) (15.98 and 18.73 kg/day) and a quadratic increase (p < 0.05) in crude protein and energy intake (2.97 kg/day and 27.52 Mcal/day at 95.4 and 88.6% substitution, respectively). Apparent DM digestibility increased (p < 0.05), but fiber digestibility decreased linearly (p < 0.05). Treatments had a quadratic effect (p < 0.05) on MY and fat-corrected MY (24.17 kg/day and 21.9 kg/day at 63.9% and 38.6% CC plus SB, respectively). Milk fat (3.26 and 2.35%) and total solids content decreased linearly (p < 0.05), whereas the percentages of protein, lactose, and nonfat solids increased (p < 0.05). Additionally, the CC–SB diets linearly reduced the time spent on feeding and rumination and total chewing time. For Holstein cows fed common semiarid diets, milk production can be maximized by replacing 38.6% of ECS with CC plus SB.
Phenotypic variation in milk fatty acid composition and its association with stearoyl-CoA desaturase 1 (SCD1) gene polymorphisms in Gir cows Carolina Guimarães Ramos Matosinho, Pablo Augusto de Souza Fonseca, Maria Gabriela Campolina Diniz Peixoto, Izinara Cruz Rosse, Fernando César Ferraz Lopes, et al. Journal of Animal Breeding and Genetics, 2023 Individual variation in milk fatty acid (FA) composition has been partially attributed to stearoyl-CoA desaturase 1 (SCD1) gene polymorphisms in taurine breeds, but much less is known for Zebu breeds. This study investigated the phenotypic variation in milk FA composition, and the influence of SCD1 variants on this trait and on milk fat desaturase indices (DI) in Gir cows. The functional impact of SCD1 variants was predicted using bioinformatics tools. Milk and blood samples were collected from 312 cows distributed in 10 herds from five states of Brazil. SCD1 variants were identified through target sequencing, and milk FA composition was determined by gas chromatography. Phenotypic variation in milk FA composition fell within the range reported for taurine breeds, with SCD18 index showing the lowest variation among the DI. Fourteen SCD1 variants were identified, six of which not previously described. Regarding the A293V polymorphism, all cows were homozygous for the C allele (coding for alanine), whereas all genotypes were detected for the second SNP affecting the 293 codon (G > A), with compelling evidence for functional effects. Significant associations (based on raw p-values) were found between this SNP and C12:0, cis-9, trans-11 CLA and short-chain FA, and between another SNP (rs523411937) and C15:0 and odd-chain linear FA. A new SNP on Chr26:21277069 was associated with trans-11 C18:1, cis-9, trans-11 CLA, C18:3 n-3 and n-3 FA. These findings indicate that SCD1 polymorphisms also contributes to the phenotypic variation in milk FA composition of Gir cows, with potential use in their breeding programmes.
Nutritional Quality of Milk Fat from Cows Fed Full-Fat Corn Germ in Diets Containing Cactus Opuntia and Sugarcane Bagasse as Forage Sources Camila S. da Silva, Marco A. S. Gama, Erick A. M. Silva, Emília F. Ribeiro, Felipe G. Souza, et al. Animals, 2023 We evaluated the performance, milk composition, and milk fatty acid profile of cows fed diets composed of cactus cladodes (Opuntia stricta [Haw.] Haw), sugarcane bagasse and increasing levels of full-fat corn germ (FFCG). We hypothesized that ground corn can be effectively replaced by FFCG when cactus cladodes and sugarcane bagasse are used as forage sources. The cows were randomly distributed into two 5 × 5 Latin Squares and fed five diets in which ground corn was progressively replaced with full-fat corn germ (FFCG; 0%, 25%, 50%, 75%, or 100% of substitution). Adding FFCG to the diet increased milk production and milk fat content and reduced milk protein content. Overall, FFCG reduced the proportion of saturated FAs and increased mono- and polyunsaturated FAs in milk, including CLA isomers. In addition, activity indices of stearoyl-CoA desaturase were reduced by increasing levels of FFCG. We conclude that the substitution of corn for FFCG in diets based on cactus cladodes and sugarcane bagasse positively modifies the FA profile of milk and could add commercial value to milk products (e.g., CLA-enriched milk). In addition, the milk fat response indicates that the basal diet was favorable to the rumen environment, preventing the trans-10 shift commonly associated with milk fat depression.
An unprotected conjugated linoleic acid (CLA) supplement reduces milk fat synthesis and forage intake in lactating goats Ruminant Physiology Digestion Metabolism and Effects of Nutrition on Reproduction and Welfare, 2023
Diet supplementation with different levels of unprotected conjugated linoleic acid (CLA) progressively decreases milk fat content and yield in dairy ewes Ruminant Physiology Digestion Metabolism and Effects of Nutrition on Reproduction and Welfare, 2023
RAPID METHOD FOR SIMULTANEOUS DETERMINATION OF CIS-9, TRANS-11 AND TRANS-10, CIS-12 CONJUGATED LINOLEIC ACID ISOMERS IN MILK BY GC-FID Brenda Porto, Isaura Faria, Jéssica de Souza, Olívia Moreira, Marco da Gama, et al. Quimica Nova, 2023 Ruminant milk is the lead source of conjugated linoleic acid (CLA) in the human diet, with cis-9, trans-11 CLA being the major among all. Small amounts of trans-10, cis-12 CLA are also found in synthetic supplements. Since both isomers are biologically active with potential health benefits, there is great interest in quantifying them for quality control routines. An alternative method for the analysis of the aforementioned CLAs by fast gas chromatography (GC) is discussed in the present study. The fatty acid methyl ester mixture obtained by alkaline catalysis was injected into a GC equipped with a flame ionization detector (FID) and fitted with an ionic liquid SLB-IL111 chromatographic column (15 m × 0.10 mm × 0.08 µm). Separation was achieved in less than 5 min using a 168 °C isotherm run. Both CLA isomers were quantified by using of single point standard addition statistical approach. Results were contested to those obtained using the recommended 100 m long CP-Sil88 capillary column and none evidence of significant differences was found within 95% confidence interval. Therefore, the proposed method could be valuable to focused regulatory routines of large numbers of samples with greater analytical frequency.
Milk fatty acid profile from dairy cows fed increasing levels of soybean oil in diets based on tropical forage Ruminant Physiology Digestion Metabolism and Effects of Nutrition on Reproduction and Welfare, 2023
Milk fat as a structuring agent of plastic lipid bases Rodolfo Lázaro Soares Viriato, Mayara de Souza Queirós, Marco Antonio Sundfeld da Gama, Ana Paula Badan Ribeiro, Mirna Lúcia Gigante Food Research International, 2018
Evaluation of polyvinyl alcohol for fatty acid supplementation in adipose tissue explant culture Genetics and Molecular Research, 2007
RECENT SCHOLAR PUBLICATIONS
Genetic Parameters Reveal Opportunities for Selection of Milk Fatty Acids in Gir and Guzerá Cows AF Reis, PS Lopes, R Veroneze, ER Carrara, MAS Gama, FAT Bruneli, ... Ruminants 5 (4), 59 , 2025 2025
Genetic parameters for milk fatty acid profiles in Gir and Guzerá cows using a Bayesian approach AF dos Reis, PS Lopes, R Veroneze, ER Carrara, FAT Bruneli, ... 2025
Feeding Cactus ( Opuntia stricta [Haw.] Haw.) Cladodes as a Partial Substitute for Elephant Grass ( Pennisetum purpureum Schum.) Induces Beneficial Changes in … AMN Corrêa, CS Silva, MAS Gama, LFP Soares, AF De Souza, ... Dairy 6 (3), 23 , 2025 2025 Citations: 1
Full-fat corn germ improves the performance and milk fat yield of Girolando cows fed sugarcane bagasse and cactus cladodes as forage sources CS da Silva, MAS da Gama, EAM Silva, EF Ribeiro, SB Felix, ... Tropical Animal Health and Production 56 (2), 104 , 2024 2024 Citations: 3
Cactus Cladodes and Sugarcane Bagasse Can Partially Replace Earless Corn Silage in Diets of Lactating Dairy Cows IPS Medeiros, SI Guido, MAS Gama, CHM Silva, MCB Siqueira, CS Silva, ... Dairy 5 (1), 33-43 , 2023 2023 Citations: 6
Fatty acid profile of Greek yogurt with colostrum addition RD da Silva GOMES, I de Macedo BARBOSA, CVDM RIBEIRO, K ANAYA, ... Food Science and Technology 43 , 2023 2023 Citations: 2
Phenotypic variation in milk fatty acid composition and its association with stearoyl‐CoA desaturase 1 ( SCD1 ) gene polymorphisms in Gir cows CGR Matosinho, PAS Fonseca, MGCD Peixoto, IC Rosse, FCF Lopes, ... Journal of Animal Breeding and Genetics 140 (5), 532-548 , 2023 2023 Citations: 12
RAPID METHOD FOR SIMULTANEOUS DETERMINATION OF CIS- 9, TRANS- 11 AND TRANS- 10, CIS- 12 CONJUGATED LINOLEIC ACID ISOMERS IN MILK BY … BLS Porto, IDL Faria, JCQ Souza, OBO Moreira, MAS Gama, MAL Oliveira Química Nova 46 (7), 743-746 , 2023 2023 Citations: 1
Full-fat corn germ improves the performance and milk quality of lactating cows fed sugarcane bagasse and cactus cladodes as forage sources C Silva, MAS da Gama, EAM Silva, EF Ribeiro, SB Felix, CCF Monteiro, ... 2023
Production, microbiological quality and nutritional aspects of artisanal cheese from Carvalhos-MG. CRT de Castro, NM Vicentini, CC Lange, FH Diniz, MAS da Gama, ... 2023
Full-fat corn germ in diets for dairy cows as an alternative to ground corn AJ Netto, MAS da Gama, SI Guido, JG Inácio, JCC Chagas, M Ramin, ... Journal of Dairy Research 90 (2), 111-117 , 2023 2023 Citations: 1
Milk fatty acid composition of cows and ewes supplemented with black wattle tannin extract KM Dias, MAS Gama, HMN Ribeiro-Filho Ciência Rural 53 (12), e20220095 , 2023 2023
Nutritional quality of milk fat from cows fed full-fat corn germ in diets containing cactus Opuntia and sugarcane bagasse as forage sources CS Silva, MAS Gama, EAM Silva, EF Ribeiro, FG Souza, CCF Monteiro, ... Animals 13 (4), 568 , 2023 2023 Citations: 7
Produção, qualidade microbiológica e aspectos nutricionais do queijo artesanal de Carvalhos-MG. CRT de CASTRO, NM VICENTINI, CC LANGE, FH DINIZ, MAS da GAMA, ... 2023
Composição em ácidos graxos no leite de vacas e ovelhas suplementadas com extrato tanífero de acácia negra KM Dias, MAS Gama, HMN Ribeiro-Filho Ciência Rural 53, e20220095 , 2023 2023
Replacing corn with full-fat corn germ in a basal diet containing cactus (Opuntia stricta) cladodes and sugarcane as forage sources induces milk fat depression associated with … MAF A.J. Netto, M.A.S. Gama, S.I. Guido, R.J.B. Bessa, J.G. Inácio, C.C.F ... Animal Feed Science and Technology 288, 115289 , 2022 2022 Citations: 10
Physicochemical, nutritional, and sensory attributes of Minas frescal cheese from grazing cows fed a supplement containing different levels of babassu coconut (Orbignya speciosa) DL Maciel, JAC Vargas, R Mezzomo, MAS da Gama, LC Leite, ... International Dairy Journal 127, 105176 , 2022 2022 Citations: 8
Partial replacement of sugarcane with cactus (Opuntia stricta) cladodes improves milk yield and composition in Holstein dairy cows BMS Sánchez, ASC Véras, EV Freitas, LR Farias, J Albuquerque, ... Animal Production Science 62 (7), 691-699 , 2022 2022 Citations: 4
Productive responses of dairy goats fed on diets containing elephant grass (Pennisetum purpureum) associated or not with cactus (Opuntia stricta) cladodes, and extra-fat whole … VJL Galeano, CCF Monteiro, FFR Carvalho, AF Souza, FG Souza, ... Small Ruminant Research 207, 106609 , 2022 2022 Citations: 13
Perfil de ácidos graxos de iogurtes tipo grego com diferentes níveis de inclusão de colostro. RDS GOMES, AHN RANGEL, C RIBEIRO, IM BARBOSA, EGSO SILVA, ... 2022 Citations: 1
MOST CITED SCHOLAR PUBLICATIONS
Diet-induced milk fat depression: association with changes in milk fatty acid composition and fluidity of milk fat DPD Gama, M. A. S., Garnsworthy, P. C., Griinari, M., Leme, P. R., Rodrigues ... Livestock Science 115, 319-331 , 2008 2008 Citations: 103
Effects of a high-energy diet on oocyte quality and in vitro embryo production in Bos indicus and Bos taurus cows JNS Sales, LT Iguma, R Batista, CCR Quintão, MAS Gama, C Freitas, ... Journal of dairy science 98 (5), 3086-3099 , 2015 2015 Citations: 101
Intake of butter naturally enriched with cis9, trans11 conjugated linoleic acid reduces systemic inflammatory mediators in healthy young adults LA Penedo, JC Nunes, MAS Gama, PEC Leite, TF Quirico-Santos, ... The Journal of nutritional biochemistry 24 (12), 2144-2151 , 2013 2013 Citations: 98
Tithonia diversifolia as a Supplementary Feed for Dairy Cows RS Ribeiro, SA Terry, JP Sacramento, SR Silveira, CBP Bento, ... PloS one 11 (12), e0165751 , 2016 2016 Citations: 61
Bovine colostrum: A source of bioactive compounds for prevention and treatment of gastrointestinal disorders RDS Gomes, K Anaya, ABS Galdino, JPF Oliveira, MAS Gama, ... NFS journal 25, 1-11 , 2021 2021 Citations: 57
Conjugated linoleic acid prevents damage caused by intestinal mucositis induced by 5-fluorouracil in an experimental model PAV de Barros, MER Andrade, S de Vasconcelos Generoso, ... Biomedicine & Pharmacotherapy 103, 1567-1576 , 2018 2018 Citations: 53
Butter naturally enriched in cis -9, trans -11 CLA prevents hyperinsulinemia and increases both serum HDL cholesterol and triacylglycerol levels in rats MM de Almeida, SCPD Luquetti, CM Sabarense, JOA Corrêa, ... Lipids in Health and Disease 13 (1), 200 , 2014 2014 Citations: 51
Milk fat as a structuring agent of plastic lipid bases RLS Viriato, M de Souza Queirós, MAS da Gama, APB Ribeiro, ... Food Research International 111, 120-129 , 2018 2018 Citations: 47
Orelha de Elefante Mexicana ( Opuntia stricta [Haw.] Haw.) spineless cactus as an option in crossbred dairy cattle diet RC Silva, JG Inácio, MA Ferreira, JCV Oliveira, DC Santos, MAS Gama, ... South African Journal of Animal Science 48 (3), 516-525 , 2018 2018 Citations: 43
A rumen unprotected conjugated linoleic acid supplement inhibits milk fat synthesis and improves energy balance in lactating goats M Baldin, MAS Gama, R Dresch, KJ Harvatine, DE Oliveira Journal of Animal Science 91 (7), 3305-3314 , 2013 2013 Citations: 42
First Report of Pectobacterium aroidearum and Pectobacterium carotovorum subsp. brasiliensis Causing Soft Rot of Cucurbita pepo in Brazil AJG Moraes, EB Souza, RLR Mariano, AMF Silva, NB Lima, AR Peixoto, ... Plant Disease 101 (2), 379 , 2017 2017 Citations: 36
Trans -10, cis -12 conjugated linoleic acid reduces neutral lipid content and may affect cryotolerance of in vitro- produced crossbred bovine embryos RITP Batista, NRB Raposo, PHA Campos-Junior, MM Pereira, ... Journal of Animal Science and Biotechnology 5 (1), 33 , 2014 2014 Citations: 36
Conjugated linoleic acid-enriched butter improved memory and up-regulated phospholipase A 2 encoding-genes in rat brain tissue MAS Gama, NRB Raposo, FB Mury, FCF Lopes, E Dias-Neto, LL Talib, ... Journal of Neural Transmission 122 (10), 1371-1380 , 2015 2015 Citations: 35
CLA induced milk fat depression reduced dry matter intake and improved energy balance in dairy goats M Baldin, R Dresch, J Souza, D Fernandes, MAS Gama, KJ Harvatine, ... Small Ruminant Research 116 (1), 44-50 , 2014 2014 Citations: 34
Manual de bovinocultura de leite AM AUAD, AMB SANTOS, AV CARNEIRO, A RIBEIRO, AC CARVALHO, ... Brasília: LK editora , 2010 2010 Citations: 32
An unprotected conjugated linoleic acid supplement decreases milk production and secretion of milk components in grazing dairy ewes DE Oliveira, MAS Gama, D Fernandes, LO Tedeschi, DE Bauman Journal of Dairy Science 95 (3), 1437-1446 , 2012 2012 Citations: 28
Desempenho produtivo e perfil de ácidos graxos do leite de vacas que receberam níveis crescentes de óleo de girassol em dietas à base de capim-elefante CGS Ribeiro, FCF Lopes, MAS Gama, MJF Morenz, NM Rodriguez Arquivo Brasileiro de Medicina Veterinária e Zootecnia 66 (5), 1513-1521 , 2014 2014 Citations: 27
Partially replacing sorghum silage with cactus ( Opuntia stricta ) cladodes in a soybean oil‐supplemented diet markedly increases trans ‐11 18:1, cis ‐9, trans ‐11 … MAS Gama, TA de Paula, ASC Véras, SI Guido, CAV Borges, ... Journal of animal physiology and animal nutrition 105 (2), 232-246 , 2021 2021 Citations: 26
Total fatty acids in Murrah buffaloes milk on commercial farms in Brazil SADA Fernandes, WRS Mattos, SV Matarazzo, H Tonhati, MAS Gama, ... Italian Journal of Animal Science 6 (sup2), 1063-1066 , 2007 2007 Citations: 23
Cis-9, trans-11 and trans-10, cis-12 CLA mixture does not change body composition, induces insulin resistance and increases serum HDL cholesterol level in rats MM de Almeida, YO de Souza, SCPD Luquetti, CM Sabarense, ... Journal of oleo science 64 (5), 539-551 , 2015 2015 Citations: 19
