Akhil Kumar Gupta

@luvas.edu.in

Scientist, Department of Veterinary Microbiology, LUVAS
Lala Lajpat Rai University of Veterinary and Animal Sciences

Akhil Kumar Gupta


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https://researchid.co/drakki1984

RESEARCH INTERESTS

Antibody production and Diagnostics
19

Scopus Publications

Scopus Publications

  • Interleukin- 17A as an Immunomodulatory Cytokine in Animal Health and Diseases: A Systematic Review
    Anita Dalal, Gajendra Singh, Akhil K. Gupta, Naresh Kumar Kakker, Sonu Kumari
    Veterinary Immunology and Immunopathology, 2026
  • Molecular insights into astrovirus, kobuvirus and rotavirus a coinfection in pigs (Sus domesticus) from Haryana, India
    Ritu Panghal, Parveen Kumar, Sanjeevna K. Minhas, Rekha Yadav, Raman Mehtani, Deepika Sheoran, Sweety Kalonia, Akhil Kumar Gupta, Mahavir Singh, Rajesh Chhabra, Anupama Dahiya, Naresh Jindal
    Tropical Animal Health and Production, 2026
  • Identification and genetic characterization of porcine rotavirus A in Haryana, India: molecular diversity and relationship with human rotavirus A
    Deepika Sheoran, Vandna Bhanot, Sanjeevna K. Minhas, Parveen Kumar, Ritu Panghal, Raman Mehtani, Akhil K. Gupta, Pawan Kumar, Rajesh Chhabra
    Journal of Veterinary Diagnostic Investigation, 2026
    Rotavirus A (RVA; family Sedoreoviridae , taxon species Rotavirus alphagastroenteritidis ) is a non-enveloped double-stranded RNA virus that has been reported from both diarrheic and non-diarrheic pigs worldwide. With significant morbidity and mortality rates in neonatal piglets, rotavirus-associated illness adds enormous economic losses to the pig industry. Furthermore, the proximity of humans and pigs facilitates cross-species infection, which results in the formation of novel strains through genetic recombination. We aimed to detect and characterize porcine RVA (PRVA) in Haryana, India, using reverse-transcription PCR targeting the VP6 , VP4 , and VP7 genes. We detected 46 of 137 (34%) rectal swab samples as positive for PRVA, including 27 of 63 (43%) from diarrheic pigs and 19 of 74 (26%) from non-diarrheic pigs. In addition, phylogenetic analysis revealed the presence of genotypes I1, I5, P[13], P[6], G11, G4, and combinations of G4P[6], G4P[13], and G11P[13] in the pig population of Haryana. G4P[6] was the most common combination found, followed by G11P[13] and G4P[13]. Genotype G11 and the combinations G4P[13] and G11P[13] have not been reported previously in pigs, in India, to our knowledge. Our finding of various genotypes, and their genetic proximity to human RVA, indicates their potential zoonotic importance.
  • Whole genome sequencing and molecular characterization of porcine astrovirus from Haryana, India
    Ritu Panghal, Parveen Kumar, Sanjeevna K. Minhas, Raman Mehtani, Sweety Kalonia, Deepika Sheoran, Pawan Kumar, Akhil K. Gupta, Rajesh Chhabra, Naresh Jindal
    Infection Genetics and Evolution, 2025
    Porcine astrovirus (PAstV) is an enteropathogen that belongs to the family Astroviridae and the genus Mamastrovirus, and is divided into five distinct genotypes (PAstV1-PAstV5). PAstV has been reported in pigs from several countries worldwide. In India, there are limited reports of genetic characterization of PAstV. The present study aimed to characterize the whole genome of PAstV from Haryana, a state in Northern India. Initially, 70 porcine fecal samples were screened for the presence of PAstV using RT-PCR that targeted the partial RdRp region. Three PAstV-positive samples were selected and their complete genomes successfully amplified by RT-PCR in two overlapping segments: segment A (∼3.6 kb) and segment B (∼3.1 kb). The phylogenetic analysis of PAstV, based on complete nucleotide sequences, revealed that PV247173 and PV247174 clustered within the PAstV1 group, whereas PV455027 clustered within the PAstV4 group. In contrast, analysis of the ORF2 amino acid sequence revealed that only PV247173 was classified in PAstV1, while PV455027 and PV247174 were classified in PAstV4. This study reports the first whole-genome molecular characterization of PAstV from Northern India.
  • H-Y antigen-based immuno-segregation strategies for bovine X sperm enrichment
    Richa Khirbat, Trilok Nanda, Aman Kumar, Meenakshi Virmani, Akhil Kumar Gupta, Jatin, Sushila Maan, Vinay G. Joshi
    Theriogenology, 2025
  • Phylogenomic analysis of bovine astrovirus expands the known repertoire of mamastrovirus genotypes in northern India
    Raman Mehtani, Parveen Kumar, Sanjeevna K. Minhas, Ritu Panghal, Anshul Lather, Rajeev Kumar, Puneet Singh, Deepika Sheoran, Akhil K. Gupta, Rajesh Chhabra, Naresh Jindal
    Archives of Virology, 2025
  • Molecular and Structural Insights into Buffalo Interleukin-17A
    Savita Budania, Surinder Kumar Kadian, Karuppiah Kanagarajadurai, Vikas Yadav, Aman Kumar, Akhil Kumar Gupta
    Journal of Interferon and Cytokine Research, 2024
    Interleukin-17A is a pro-inflammatory cytokine that plays a key role in the immune response to many pathogens and implicated in autoimmune diseases. This molecule is also involved in providing protection to many bacterial and fungal infections of gastro-intestinal tract and respiratory mucosa. Although molecular aspect of IL-17A has been studied in few species, no data are available for buffalo, which is one of the major sources of milk production in India. Therefore, in the present study, IL-17A gene of Indian Murrah Buffalo origin was cloned, expressed, and analyzed using bioinformatic tools. The coding sequence of buffalo IL-17A gene was cloned in prokaryotic expression vector (pET-28a) followed by its expression, purification, and characterization. A computational analysis was performed to understand the sequence, structure, and evolutionary relationship of buIL-17A. It revealed that the length of buIL-17A sequence without signal peptide is 132 amino acids as in cattle. However, sequence identity is found to be 99% due to one amino substitution difference between buffalo and cattle. After analysis, it can be concluded that buIL-17A recombinant protein can be used as a potential immunobiological reagent for diagnostic and therapeutic purpose.
  • Characterization of Foot-and-Mouth Disease Virus Serotype O-Specific Single Domain Antibody Expressed in the pET Expression System
    Vijay Kumar, Swati Dahiya, Savita Budania, Akhil Kumar Gupta, Punesh Sangwan, Anshul Lather, Pawan Kumar, Naresh Kumar Kakker, Ajit Singh
    Indian Journal of Microbiology, 2023
  • Persistence of maternal antibodies in calves born of combined foot-and-mouth disease + haemorrhagic septicaemia vaccinated buffaloes at organized dairy farm
    RITU PANGHAL, SWATI DAHIYA, AKHIL KUMAR GUPTA, VISHAL SHARMA, YOGESH BANGAR, NARESH KUMAR KAKKER
    Indian Journal of Animal Sciences, 2023
    Twelve apparently healthy and recently parturiated Murrah buffaloes, vaccinated with Foot-and-mouth disease (FMD) + Haemorrhagic septicaemia (HS) combined vaccine at an organized farm along with their newborn calves were inducted for detection of antibodies in serum and colostrum against FMD virus (FMDV) serotypes O, A and Asia-1, and Pasteurella multocida using enzyme linked immunosorbent assay. The calves born to vaccinated dams showed the presence of protective maternal antibody titre (≥1.8 log10) from birth till the period of study (16th week of age) against FMDV serotypes O, A and Asia-1. The maternal antibody titre against P. multocida were protective (≥1.8 log10) till fifth day of age which became partially protective thereafter till the period of study. It is recommended to avoid vaccination of the calves before 16th week of age with FMD+HS combined vaccine.
  • Kinetics of humoral response to Pasteurella multocida in buffaloes against combined foot-and-mouth+haemorrhagic septicaemia vaccine
    NEENA RUSTAGI, NARESH KUMAR KAKKER, SUMAN SHEORAN, PATIL CHANDRASHEKHAR SANTOSH, SWATI DAHIYA, AKHIL KUMAR GUPTA
    Indian Journal of Animal Sciences, 2023
    The present study reports kinetics of anti-P. multocida antibody (Ab) response at monthly intervals in Murrah buffaloes of different age groups vaccinated against combined Foot-and-mouth disease+haemorrhagic septicaemia (FMD+HS) vaccine. A total of 60 Murrah buffaloes of three age groups having 20 animals each: calves, heifers and adults were used to monitor anti-P. multocida Ab response at monthly intervals using single dilution indirect ELISA. The percentage of adult buffaloes protected were found to be the highest during all the six months post-vaccination followed by heifers and calves. The protective mean Ab titres were maintained up to six months post-vaccination for heifers and adults but not for calves. The F value (the ratio of two mean squares) for pre- and all the six month(s) post-vaccination and all the three age groups was significantly higher. Pearson Chi square value for pre-vaccination and all the six months except three months post-vaccination was significantly higher. Pearson correlation value was significantly higher with positive linear relationship. The data in the present study indicated that the combined FMD+HS vaccine was found to be effective in buffaloes of all age groups at government organised farm and could be an ideal approach in field conditions under Livestock Health Disease Control Program run by the Government of India.
  • Clinical Diagnosis and Therapeutic Management of Trypanosoma theileri Infection Associated with Peritonitis in Crossbred Cow: A Rare Case Report
    Maneesh Sharma, C. S. Patil, Sandeep Saharan, Akhil Kumar Gupta, Snehil Gupta, V. K. Jain
    Acta Parasitologica, 2022
  • Molecular detection and characterization reveals circulation of multiple genotypes of porcine astrovirus in Haryana, India
    Rajpreet Kour, Parveen Kumar, Naresh Jindal, Sanjeevna Kumari Minhas, Ramesh Kumar, Akhil Kumar Gupta, Anu Malik
    Archives of Virology, 2021
  • Molecular Cloning and Characterization of Mx2 for Early Pregnancy Diagnosis in Bubalus bubalis
    Kanisht Batra, Trilok Nanda, Aman Kumar, Akhil Kumar Gupta, Rajni Kumari, Vinay Kumar, Nancy Sheoran, Sushila Maan
    Proceedings of the National Academy of Sciences India Section B Biological Sciences, 2019
  • Real time PCR assay for differentiation of Brucella abortus and brucella melitensis
    Vinay Kumar, Sushila Maan, Aman Kumar, Kanisht Batra, Deepika Deepika, Anita Dalal, Akhil Gupta, Nitish Bansal, Nancy Sheoran, N. Maan
    Indian Journal of Animal Research, 2018
  • Novel molecular diagnostics and therapeutic tools for livestock diseases
    Sushila Maan, Sangeeta Dalal, Aman Kumar, Anita Dalal, Nitish Bansal, Deepika Chaudhary, Akhil Gupta, Narender Singh Maan
    Advances in Animal Biotechnology and Its Applications, 2018
  • Concurrent infection of Bluetongue and Peste-des-petits-ruminants virus in small ruminants in Haryana State of India
    S. Maan, Aman Kumar, A. K. Gupta, A. Dalal, D. Chaudhary, T. K. Gupta, N. Bansal, V. Kumar, K. Batra, N. Sindhu, Ankit Kumar, N. K. Mahajan, N. S. Maan, P. P. C. Mertens
    Transboundary and Emerging Diseases, 2018
  • Reverse transcription loop-mediated isothermal amplification assays for rapid identification of eastern and western strains of bluetongue virus in India
    S. Maan, N.S. Maan, K. Batra, A. Kumar, A. Gupta, Panduranga P. Rao, Divakar Hemadri, Yella Narasimha Reddy, M. Guimera, M.N. Belaganahalli, P.P.C. Mertens
    Journal of Virological Methods, 2016
  • Full-genome sequence analysis of a reassortant strain of Bluetongue virus serotype 16 from southern India
    Lalit Kumar, Kanisht Batra, Deepika Chaudhary, Akhil Kumar Gupta, Anita Dalal, Brindha Kalyanaraman, Ganesan P. Irulappan, Vinay Kumar, Sushila Maan
    Genome Announcements, 2016
  • Production and characterization of monoclonal antibodies against west Nile virus
    Veterinary Practitioner, 2013