Muhammad Naveed currently works at the University of Minnesota Duluth, United States. Naveed conducted research in "brain and neurosciences" and heart failure.
Hosts Genetic Diversity of SARS-CoV-2 Kashif Prince, Arooj Fatima, Sana Tehseen, Muhammad Sajjad Khan, Muhammad Saeed, et al. Genetic Diversity of Coronaviruses from Sarscov to Sars Cov 2 Part 2, 2025 The coronavirus disease-19 (COVID-19) spread worldwide in no time. Finally, the World Health Organization declared it a pandemic in March 2020. The severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) can mutate, and many mutations have been observed worldwide. The severity of symptoms varies from mild to critical cases, and the incubation period ranges from 5-14 days. Various studies have shown that the diversity of SARS-CoV-2 within the hosts is prevalent, and some genomes are more susceptible to the alterations due to mutation. Some of the tissues that exhibited the highest ACE2 expression in different host tissues (humans) were kidneys, thyroid, heart, adipose tissue, small intestine, and testicles. Endothelial cells have also been the site for SARS-CoV-2. Chinese people were the first to be reported with the polymorphism detection for the ACE2 gene. Different variants of the ACE2 gene that are closely linked with hypertension were rs464155, rs4240157, and rs4830542. There has been a close association between ACE2 and TMRSS2 and SARS-COV-2, SARS-CoV-1, and influenza virus. The inducibility of heme oxygenase-1 (HO-1) enzyme to reactive oxygen species is regulated by the GT dinucleotide repeat mutation and polymorphism of the HO-1 gene.
Mechanisms of the Ershiwuwei Guijiu Pill in Treating Postmenopausal Osteoporosis Based on Network Analysis and Experimental Validation Fanglin Duan, Li-Xue Zhang, Muhammad Naveed, Peifeng Wu, Yao Yu, et al. Journal of Evidence Based Integrative Medicine, 2025 Background The Tibetan medicine Ershiwuwei Guijiu Pill (EWGP), a classic Tibetan medicine prescription for the treatment of postmenopausal osteoporosis (PMOP) in the Qinghai–Tibet region, has attracted extensive attention due to its curative effects on gynecological diseases. However, its chemical ingredients and molecular mechanism are still unclear. Aim of the study To analyze the chemical constituents and effective serum chemical metabolites of EWGP and to explore the molecular mechanism of EWGP in treating PMOP through network analysis and experimental validation. Methods The ethanol extract of EWGP and its drug-containing serum were detected by liquid chromatography-mass spectrometry (LC–MS), and the chemical constituents were analyzed and identified. SwissTarget prediction was used to predict the corresponding potential target genes of the identified chemical components. Thereafter, a visualization network of the components and corresponding targets was constructed with Cytoscape software. Moreover, a specific disease database for animals was used to search and filter for osteoporosis (OP) targets, and a drug-disease target protein–protein interaction (PPI) network was constructed. Cytoscape 3.7.0 was used for visualization and cluster analysis, and R Studio was used for GO and KEGG enrichment analysis. AutoDock Tools were applied for molecular docking of the serum metabolites and specific target proteins. The potential mechanism of EWGP in preventing and treating PMOP was predicted by network pharmacology analysis and was experimentally studied and verified in vivo and in vitro . Results A total of 199 chemical substances were identified in the ethanol extract, and 11 were found in the serum. A total of 419 predicted targets and 128 target genes related to osteoporosis were screened. There were 16 common targets identified between the predicted targets and OP genes. Following the enrichment analysis, 16 KEGG signaling pathways and 63 GO biological process items were identified. The results of molecular docking showed that the main active compounds may be Protopine, Hetisine, Piperine, Visaminol, Boldine, and Trigonelline, and the specific targets may be CYP17A1, ESR2, MAPK14, and the vitamin D receptor (VDR). The results of cell and animal experiments showed that EWGP may improve bone metabolism via estrogen and calcium signaling pathways regulated by estrogens and calcium ions. Conclusions EWGP contains multiple herbal drugs and treats PMOP through multiple targets and signaling pathways. We preliminarily tested the chemical compounds of EWGP, especially in the serum, to determine the chemical metabolites of EWGP and revealed the molecular mechanism of EWGP in preventing and treating PMOP; moreover, we used computer-virtual molecular docking and experiments for preliminary verification of the core targets of network pharmacology analysis.
Urinary polycyclic aromatic hydrocarbons and adult obesity among the US population: NHANES 2003–2016 Manthar Ali Mallah, Jennifer W. Hill, Bidusha Neupane, Muhammad Zia Ahmad, Mukhtiar Ali, et al. Clinical Obesity, 2024 SummaryPolycyclic aromatic hydrocarbons (PAHs) are naturally occurring environmental pollutants that may contribute to obesity in the adult population. To investigate the relationship between the urinary concentrations of PAH metabolites and adult obesity among the US population, the National Health and Nutritional Examination Survey (NHANES, 2003–2016) was used as a data source for this study. As many as 4464 participants in the NHANES 2003–2016 were included in the final analyses. We used logistic regression to look at the link between urinary PAH metabolites and obesity, using odds ratios (ORs) and 95% confidence intervals (CIs). The study sample comprised 4464 individuals aged ≥18 years, 2199 were male and 2265 were female. The study characteristics for four different quartiles were analyzed, and the average ages of the four urinary PAH quartiles were 49.61 ± 20.01, 46.63 ± 20.33, 44.28 ± 19.19, and 43.27 ± 17.68 years, respectively. In the quartile analysis of all participants, the third quartile was significantly associated with an increased prevalence of obesity (OR = 1.33, 95% CI = 1.12–1.59) with p‐values <.05. In addition, females, but not males, had a strong link between the second, third, and fourth quartiles of urinary PAH and a higher risk of obesity (OR = 1.27, 95% CI = 1.00–1.61; OR = 1.52, 95% CI = 1.19–1.94; and OR = 1.39, 95% CI = 1.09–1.78). In conclusion, the study observed that urinary PAH metabolites were associated with the prevalence of obesity among the US population.
Molecular Epidemiological Analysis of SARS-CoV Sana Tehseen, Sidra-Tul- Muntaha, Muhammad Sajjad Khan, Muhammad Saeed, Muhammad Naveed, et al. Genetic Diversity of Coronaviruses Volume 1 from Sars Cov to Mars Cov, 2024
