Assistant Professor, Department of Veterinary Medicine, College of Veterinary Sciences, LUVAS Lala Lajpat Rai University of Veterinary and Animal sciences
AN INSIGHT INTO DIAGNOSIS AND THERAPEUTIC MANAGEMENT OF DERMATOPHILOSIS IN WATER BUFFALO Gaurav Charaya, Divya Agnihotri, Jasleen Kaur, Devashish Srivastava Buffalo Bulletin, 2026 Dermatophilosis is prevalent in tropical countries and has higher incidence during high humidity and rainy season. In the present case report, a six years, non-pregnant buffalo was referred to University Clinic with the history of nodular skin lesions for one month on the entire body surface. The lesions observed on dorsum were mainly crusts and scabs and ulcers on foot. Tick infestation was also noticed. Based upon the history, clinical signs and appearance of lesions, the case was tentatively diagnosed as dermatophilosis or mite infestation or cutaneous form of theileriosis. Deep and superficial skin scrappings and blood smear examination was performed to confirm the disease. The skin scrappings under microscopy was found negative for mites. No haemoparasites were observed on examination of blood smear. Skin scraps were kept in normal saline solution overnight followed by Giemsa staining and examination revealed typical railroad appearance structure of Dermatophilus congolense. On the basis of typical morphology of bacterium, the case was diagnosed to be of dermatophilosis. For the therapeutic management of Dermatophilosis inj. oxytetracyline 10 mg/Kg intravenously for five days followed by lomg actiong oxytetracyclin for two weeks. Animal showed marked improvement after 14 days of therapy with complete clinical recovery after 20 days with the disappearance of scabs and crusts with no remission fo signs was observed for next 3 months.
Immuno-chromatography based rapid detection of canine distemper in dogs and its comparison with RT-PCR Jharna Koul, Divya Agnihotri, Ricky Jhambh, Tarun Kumar, Gaurav Charaya Veterinary Research Forum, 2025 The present study was planned to confirm the clinical diagnosis of canine distemper in dogs with reverse transcriptase polymerase chain reaction (RT-PCR) and its comparison with lateral flow assay based immuno-chromatographic (IC) technique. Fifty clients owned dogs having clinical signs suggestive of respiratory, skin or nervous form of canine distemper were included in the study. An immuno-chromatography-based test was applied using serum to screen each of the suspected dog. In addition to serum, nasal discharges of 15 cases and ocular discharges of 10 samples were used to diagnose canine distemper. Screening with IC kit revealed 72.00% serum samples positive, 66.66% ocular and 50.00% nasal samples were found positive for antigen. The RT-PCR targeting N gene of canine distemper virus was used for the molecular diagnosis of canine distemper. Out of 50 blood samples tested with RT-PCR assay, 38 (76.00%) samples were positive showing characteristics band of 287bp. Statistical comparison of IC Kit (serum samples) results with RT-PCR results comparison showed that IC kit was 52.00% accurate with 36.84% sensitivity, 100% specificity, positive predictive value of 93.33% and negative predictive value of 31.43%. In the current study it was observed that the IC test was rapid, quick and specific but was found to be less sensitive compared to RT-PCR.
Statistical evaluation of modalities for diagnosis of Anaplasmosis in dairy cattle RAVINA, Y SINGH, J KAUR, R JHAMBH, G CHARAYA Indian Journal of Animal Sciences, 2025 Anaplasmosis is an important tick borne rickettsial disease affecting bovines. Disease diagnosis plays an important role in limiting the spread of infection because of persistence of its carrier state. Conventional microscopy of stained blood smear, serological assay and molecular tests are commonly used for the diagnosis of anaplasmosis. The present study was planned to compare different diagnostic methods for detection of Anaplasma spp. in blood samples of cattle. A total of 72 blood and serum samples were collected from cattle suspected to be suffering from anaplasmosis. Paired stained blood smear was examined for characteristic dot shaped intraerythrocytic form of organism. Serum samples were processed for competitive ELISA for the presence of antibodies to Anaplasma spp. DNA was extracted and subjected to PCR assay for detection of Anaplasma marginale targeting msp4 gene. Out of 72 samples, 91.66% of cases were found to be positive by cELISA, followed by 56.94% by PCR assay and only 25% by microscopy. Microscopy was found to be 100% specific whereas ELISA was found to be 100% sensitive with microscopy showing 100% positive predictive value and ELISA showing 100% negative predictive value. For early and rapid detection, PCR assay should be employed, while for rapid screening of herd and control strategies cELISA may be used but in routine practice, microscopic examination can suffice.
Subclinical mastitis and its management Gaurav Charaya, Jasleen Kaur, Divya Agnihotri, Priyanka Gahlot Handbook of Milk Production Quality and Nutrition, 2025
Clinical mastitis and its management Gaurav Charaya, Jasleen Kaur, Avni Chauhan, Divya Agnihotri Handbook of Milk Production Quality and Nutrition, 2025
Colibacillosis Gaurav Charaya, Jasleen Kaur, Yudhbir Rana Handbook of Zoonotic Diseases of Goats, 2024 Colibacillosis is a significant disease affecting goat kids, leading to severe economic losses due to high morbidity and mortality rates. The disease is caused by Escherichia coli, which is a normal inhabitant of the intestinal tract, but certain strains can cause disease, especially in young animals, characterized by diarrhoea. Goat kids born in litters, particularly triplets, are more susceptible. Disease incidence decreases with age, due to the development of resistance in intestinal cells. Transmission occurs through the faecal–oral route, exacerbated in crowded conditions. Serotyping distinguishes E. coli strains based on surface antigens. Different pathotypes, including enteropathogenic and uropathogenic, cause varying types of diarrhoeal illnesses. Diagnosis and distinguishing various serotypes can be done by serological and molecular assays. Treatment typically involves antibiotics, but alternative approaches like herbal remedies, probiotics and homeopathy are explored to mitigate antibiotic resistance. Prevention focuses on sanitation, hygiene, vaccination and passive immunization through colostrum intake.
Production Diseases of Goats Gaurav Charaya, Jasleen Kaur, Chinmoy Maji, Tanmoy Rana Principles of Goat Disease and Prevention, 2023 This chapter mainly focuses on production diseases of goats. Body condition score (BCS) and criteria for its evaluation are highlighted. Maintaining a BCS around 3 on a scale of 1–5 is important for the prevention of production diseases. Overfed/obese goats with a BCS of more than 3 and undernourished goats with a BCS of less than 2 are prone to pregnancy toxemia. Production diseases explained in the chapter are periparturient diseases of goats and are mainly a result of a disbalance of the metabolism. In goats, nutrient requirements remain stable throughout different phases except when they are near to kidding and this period is crucial for the herder to manage the flock. Pregnancy toxemia, hypomagnesemic tetany, and milk fever are the major production diseases in goats. The major biochemical alterations observed are hypoglycemia, hypomagnesemia, and hypocalcemia, respectively, in these three diseases. With proper management practices adopted by the herder, these diseases can be prevented.
CONCURRENT HEPATOZOONOSIS AND COCCIDIOSIS IN A PUP: A CASE REPORT Veterinary Practitioner, 2022
COMPARISON OF PARASITOLOGICAL, SEROLOGICAL AND MOLECULAR DIAGNOSTIC TESTS FOR DETECTION OF SUBCLINICAL TRYPANOSOMOSIS IN TWO ORGANISED DAIRY HERDS Buffalo Bulletin, 2022
Relative expression of proinflammatory cytokines by real time PCR in milk somatic cells of subclinical mastitis affected buffaloes Indian Journal of Animal Sciences, 2016
ELIMINATING SUBCLINICAL URINARY TRACT INFECTION AMELIORATES PRODUCTION AND REPRODUCTION PERFORMANCE IN BUFFALOES G Charaya, S Punia, A Kumar Buffalo Bulletin 45 (1) , 2026 2026
AN INSIGHT INTO DIAGNOSIS AND THERAPEUTIC MANAGEMENT OF DERMATOPHILOSIS IN WATER BUFFALO G Charaya, D Agnihotri, J Kaur, D Srivastava Buffalo Bulletin 45 (1), 21-27 , 2026 2026
Statistical evaluation of modalities for diagnosis of Anaplasmosis in dairy cattle Y SINGH, J KAUR, R JHAMBH, G CHARAYA Indian Journal of Animal Sciences 95 (8) , 2026 2026
Immuno-chromatography based rapid detection of canine distemper in dogs and its comparison with RT-PCR J Koul, D Agnihotri, R Jhambh, T Kumar, G Charaya Veterinary Research Forum 16 (5), 261 , 2025 2025
Therapeutic Management of Cattle Suffering From Anaplasmosis YS Ravina, R Jhambh, G Charaya 2025
Subclinical mastitis and its management G Charaya, J Kaur, D Agnihotri, P Gahlot Handbook of Milk Production, Quality and Nutrition, 201-211 , 2025 2025
Clinical mastitis and its management G Charaya, J Kaur, A Chauhan, D Agnihotri Handbook of Milk Production, Quality and Nutrition, 193-200 , 2025 2025
14 Colibacillosis G Charaya, J Kaur, Y Rana The Handbook of Zoonotic Diseases of Goats, 172 , 2024 2024 Citations: 1
Symmetric Dimethylarginine (SDMA) as Biomarker of Chronic Kidney Diseases in Dogs. A Kumar, G Charaya Indian Journal of Veterinary Sciences & Biotechnology 20 (1) , 2024 2024
Atypical theileriosis: cutaneous form in a cow and its therapeutic management G Charaya, Ravina, D Lather Comparative Clinical Pathology 32 (6), 895-898 , 2023 2023
Production Diseases of Goats G Charaya, J Kaur, C Maji, T Rana Principles of Goat Disease and Prevention, 155-164 , 2023 2023
Application of trans tracheal wash in diagnosis and therapeutic management of chronic pneumonia in buffalo. P Kumar, G Charaya, J Kaur, D Lather, R Chhabra, YS Rana 2023
Concurrent hepatozoonosis and coccidiosis in a pup: a case report. P Kumar, D Agnihotri, M Sharma, G Charaya, K Batra, YS Rana Veterinary Practitioner 23 (2) , 2022 2022
Comparison of parasitological, serological and molecular diagnostic tests for detection of subclinical Trypanosomosis in two organised dairy herds G Charaya, NK Rakha, A Kumar, AK Gupta, S Maan, A Singh, P Goel Buffalo Bulletin 41 (2), 241-249 , 2022 2022 Citations: 1
Subclinical urinary tract infection in Murrah buffalo herd: point prevalence, bacterial etiology and antibiogram. S Punia, A Kumar, G Charaya, Nitesh 2022
Ultrasonographic Findings of Urinary System in Dogs suffering from Chronic Renal Failure A Kumar, T Kumar, G Charaya Journal of Animal Research 11 (6), 1039-1042 , 2021 2021
An outbreak of theileriosis in a herd of crossbred cattle and Murrah buffaloes and its managment. G Charaya, N Jindal, V Bhanot, A Kumar, P Goel 2021 Citations: 2
End point multiplex PCR for diagnosis of haemoprotozoan diseases in cattle G Charaya, NK Rakha, A Kumar, S Maan, P Goel Acta Parasitologica 66 (1), 91-97 , 2021 2021 Citations: 6
Antimicrobial resistance profiling of Staphylococcus aureus isolated from mastitic milk of bovine and dairy environment from arid and semi-arid regions of India. T Bhati, R Chhabra, R Yadav, G Charaya, AK Kataria 2021
Haematobiochemical alterations in crossbred cattle suffering from theileriosis G Charaya, P Goel, J Kaur, S Punia, Y Bangar, Y Singh Indian J Vet Med 41 (2), 22-26 , 2021 2021 Citations: 3
MOST CITED SCHOLAR PUBLICATIONS
Emerging status of anaplasmosis in cattle in Hisar T Kumar, N Sindhu, G Charaya, A Kumar, P Kumar, G Chandratere, ... Veterinary world 8 (6), 768 , 2015 2015.0 Citations: 54
Pathogens isolated from clinical mastitis in Murrah buffaloes and their antibiogram G Charaya, A Sharma, A Kumar, M Singh, P Goel Veterinary World 7 (11) , 2014 2014.0 Citations: 31
Pathogens isolated from clinical cases of urinary tract infection in dogs and their antibiogram M Punia, A Kumar, G Charaya, T Kumar Veterinary World 11 (8), 1037 , 2018 2018.0 Citations: 29
Comparative evaluation of polymerase chain reaction assay with microscopy for detection of asymptomatic carrier state of theileriosis in a herd of crossbred cattle G Charaya, NK Rakha, S Maan, A Kumar, T Kumar, R Jhambh Veterinary World 9 (9), 1039 , 2016 2016.0 Citations: 27
Prevalence, etiology and antibiogram of bacterial isolates recovered from mastitis of buffaloes A Sharma, R Chhabra, M Singh, G Charaya Buffalo Bulletin 37 (3), 313-320 , 2018 2018.0 Citations: 22
Detection of major mastitis pathogens by multiplex polymerase chain reaction assay in buffalo milk G Charaya, A Sharma, A Kumar, P Goel, M Singh INDIAN JOURNAL OF ANIMAL SCIENCES 85 (2), 122-125 , 2015 2015.0 Citations: 21
Molecular occurrence and therapeutic management of canine parvovirus infection in dogs D Kataria, D Agnihotri, V Jain, G Charaya, Y Singh International Journal of Current Microbiology and Applied Sciences 9 (2 … , 2020 2020.0 Citations: 17
Sridhar (2015). Incidence and clinical vital parameters in primary ketosis of murrah buffaloes A Kumar, N Sindhu, P Kumar, T Kumar, G Charaya, VK Jain Veterinary World 8 (9), 1083-1087 , 2015 2015.0 Citations: 17
Haemato-biochemical alterations in dogs suffering from chronic renal failure AK Sonu, G Charaya, Y Bangar, D Agnihotri, T Kumar Indian J Vet Med 39 (1), 31-35 , 2019 2019.0 Citations: 15
Pankaj and Kumar, A.(2013) Subclinical mastitis at an organised farm: prevalence, etiology and antibiogram GC Charaya, A Sharma, M Singh, S Tiwari Haryana Vet 52, 30-32 , 0 Citations: 15
Assessment of lactate dehydrogenase enzyme activity in milk as a marker for detection of subclinical mastitis M Singh, A Sharma, D Mittal, P Yadav, G Charaya Journal of Animal Research 6 (2), 113 , 2016 2016.0 Citations: 12
Evaluation of physico-chemical and microscopical changes of urine in dogs with urinary tract infection M Punia, D Gulia, A Kumar, G Charaya Haryana Vet 57 (2), 191-193 , 2018 2018.0 Citations: 10
An outbreak of contagious ecthyma with rare tongue lesions in a goat flock in haryana T Kumar, N Sindhu, A Kumar, G Charaya, A Kumar, S Maan, NK Mahajan Haryana Vet 55 (2), 185-187 , 2016 2016.0 Citations: 8
Prevalence and characterization of coagulase-negative staphylococci associated with buffalo mastitis M Singh, A Sharma, D Mittal, G Charaya Indian J Comp Microbiol Immunol Infect Dis 35, 67-72 , 2014 2014.0 Citations: 8
Antibiogram of bacteria isolated from dogs suffering from otitis externa D Agnihotri, G Charaya, R Chabbra, T Kumar, VK Jain Indian J Comp Microbiol Immunol Infect Dis 1, 15-20 , 2019 2019.0 Citations: 7
End point multiplex PCR for diagnosis of haemoprotozoan diseases in cattle G Charaya, NK Rakha, A Kumar, S Maan, P Goel Acta Parasitologica 66 (1), 91-97 , 2021 2021.0 Citations: 6
S., Agnihotri, D., Sridhar., 2015. Clinical and hemato-biochemical studies on fever of unknown origin in buffaloes. Vet P Kumar, VK Jain, A Kumar, N Sindhu, T Kumar, G Charaya, K Surbhi World 8 (10), 1225-1229 , 0 Citations: 6
Relative expression of proinflammatory cytokines by real time PCR in milk somatic cells of subclinical mastitis affected buffaloes M Singh, A Sharma, A Kumar, D Mittal, P Kumar, G Charaya The Indian Journal of Animal Sciences 86, 991-993 , 2016 2016.0 Citations: 5
Hemato-biochemical studies on clinical cases of primary Ketosis in buffaloes A Kumar, T Kumar, P Kumar, G Charaya, N Sindhu, S Kumari, R Yadav Journal of Animal Research 5 (3), 443 , 2015 2015.0 Citations: 5
Pankaj and Kumar, A.(2013) G Charaya, A Sharma, M Singh, S Tiwari Subclinicalmastitis at an organised farm: prevalence, etiology and … , 0 Citations: 5