Ajeet Kumar
@basu.org.in
Assistant professor
Bihar Animal Sciences University
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Scopus Publications
Ankit Ahuja, Mrigank Honparkhe, Ajeet Kumar, Amarjeet Bisla, and Pankaj Sood
Springer Science and Business Media LLC
Ajeet Kumar, Vatsal Mishra, Chandra Bhan Singh, Rashmi Patel, Siddharth Samrat, Madhukar Rai, Nilesh Kumar, Vijay Tilak, Vineeta Gupta, and Akhtar Ali
Springer Science and Business Media LLC
Abstract Background Chronic myeloid leukaemia (CML) is a haematopoietic stem cell disorder, caused by a balanced reciprocal translocation (t(9;22) (q34;q11)) that leads to the formation of BCR (Breakpoint Cluster Region)-ABL (Abelson) fusion transcripts known as Philadelphia (Ph) chromosome. The prevalence of BCR-ABL fusion transcripts in Indian CML population is poorly understood, and few studies have been reported from India. The aim of the present study was to determine the frequencies as well as prognostic effects of the three fusion transcripts, i.e. b2a2, b3a2 and e1a2 in an Indian population. Methods RNA was isolated from total 123 samples, 27 bone marrow (BM) samples and 96 peripheral blood (PB) samples, of CML patient followed by cDNA synthesis. Real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed using TaqMan® assay (ABI, CA, USA) to monitor BCR-ABL transcript. Results Ph' chromosome was observed in 103 patients whereas it was not detected in 20 cases. qRT-PCR revealed that the b3a2 fusion transcripts were the most common transcript in CML patients (63.41%) while b2a2 fusion transcript was present in 16.26% cases. Co-expression of b3a2 + b2a2 fusion transcript was observed in 0.81% cases whereas co-expression of b3a2 + e1a2 fusion transcript was found in 1.63% cases. There was no correlation observed between b3a2 fusion transcript and platelet count. The fusion transcript b2a2 was observed in relatively younger patients compared to b3a2 fusion transcript. Although this correlation was not statistically significant. Conclusion The co-expression of BCR-ABL fusion transcripts was higher (63.41% aggregate of b3a2) in the present population in contrast with other populations reported. This finding was consistent with the frequency data reported from Sudan.
Gyan Dev Singh, Anil Gattani, Ajeet Kumar, and Anil Kumar
Office of the Library, Kasetsart University
The minor surgical intervention and safe restraining requires sedation and analgesia especially in furious animals like buffaloes which can be achieved by using proper combination of sedative and analgesic drugs. The combination of xylazine with opioids is an effective way to achieve sedation and analgesia in cattle and horses. Six apparently healthy buffaloes having minor wounds were included in the present study. The sedation was accomplished by fentanyl (5 µg per kg b.wt) plus xylazine (0.05 mg per kg b.wt) intravenously. Physiological, hematobiochemical and electrocardiographic parameters were observed at different time interval after the administration of drugs. Following the administration of medicine, there was a significant reduction in heart rate, systolic blood pressure, diastolic blood pressure, mean arterial blood pressure, hemoglobin, and packed cell volume. Respiration rate increased significantly at 30 minutes interval. PR interval and QT interval increased significantly at 20 minutes and 15 minutes respectively of the observation period, whereas a significant increase in the duration of ST segment was noticed from 15 minutes to 30 minutes. The present study revealed that the drug combination has no deleterious effects on cardiopulmonary, hematobiochemical and physiological parameters during the observation period.
Akhtar Ali, Ajeet Kumar, Pawan K. Dubey, Vivek Pandey and Ankur Singh
Pigmentary mosaicism (PM) is a clinical condition of dyspigmentation with chromosomal abnormality. PM presents with both cutaneous and extracutaneous manifestation. Hypomelanosis of Ito and linear and whorled nevoid hypermelanosis are syndromic disorders in which PM is one of the manifestations. We present a case of a 1-year-old child with a unique constellation of symptoms of unilateral syndactyly, hemihypertrophy, and skin hyperpigmentation. Karyotype from peripheral blood was normal. We found genetic aberration (mosaic 2q35 deletion) in the present case from fibroblast cultured from the affected area. This unique constellation of symptoms was previously reported once but genetic study was not done from the affected tissue. This case highlights the need of considering fibroblast culture-based genetic study rather than doing simple karyotype from peripheral blood. Genetic study also established the molecular basis of symptoms in the above case.
Ajeet Kumar, Vijai Tilak, Disha Arora, Marisha, Rahul, Deepak Gautam, and Akhtar Ali
Elsevier BV
Paramveer Singh Sangha, Ajeet Kumar, Mrigank Honparkhe, Jasbir Singh Bedi, Ashwani Kumar Singh, and Prahlad Singh
Agricultural Research Communication Center
Background: The pesticides are potentially toxic compounds that have become omnipresent in the environment. These toxicants modulate and/or disrupt the reproductive and hormonal environment by acting on hypothalamus, pituitary and reproductive organs. Fewer studies have been carried out on pesticide residues in body fluids especially blood and semen in breeding bulls. Method: Study was done to assess the pesticide residues in blood in relation to the endocrine profile in crossbred breeding bulls. Nineteen blood samples were collected and were allowed to clot overnight; serum was separated and stored at -20°C until analysis. The samples were analyzed for the pesticide residues using gas chromatography (GC) for seven organochlorine pesticides (OCPs) namely Heptachlor epoxide, Chlordane, Fipronil, Lindane, Methoxychlor, op-DDT and Endrin and eleven organophosphorus pesticides (OPPs) namely Chorpyrifos, Dichlorovos, Ethion, Monocrotophos, Malathion, Parathion-methyl, Profenphos, Phorate, Triazophos, Quinalphos and Phosalone and four synthetic pyrethroids (SPs) namely Cypermethrin, Permethrin, Deltamethrin, Cyhalothrin. Result: The blood samples of these bulls were found positive for organophosphate-phosalone and around 73.68% of the bulls were positive for phosalone at a retention time of 13.837 min. The concentration of phosalone in the blood of crossbred breeding bulls was found to be 1.89±0.98 ng/ml. In the present study, the blood hormonal profile of pesticide residues was analyzed and it showed a significant increase in the level of Estrogen thereby decreasing the testosterone: estrogen ratio. The decreased testosterone: estrogen ratio may be attributed to the detection of phosalone in blood.
Narinder Singh, Khushpreet Singh, Gurjot Kaur Mavi, and Ajeet Kumar
ACS Publisher
The present study was conducted to investigate success of AI in relation to progesterone concentrations with respect to the timing of inseminations or heat detection in the field. Milk samples were collected for progesterone assay from 149 crossbred cows subjected to AI. The milk progesterone concentrations were estimated by RIA to access the reproductive status of the cows. On the day of AI, progesterone concentrations were less than 1.0 nmol/L (Low) in 51% samples and more than 3.00 nmol/L (High) in 18%. Rest 31 % samples showed intermediate progesterone (1.01 to 2.99 nmol/L) suggesting that these cows were nor in proper heat at the time of AI. On the basis of two samples (day 0 and day 10-12 post AI), 50% cows had low progesterone concentrations on the day of AI and high in the second sample, indicating that these cows were inseminated during estrus and ovulated subsequently. Whereas, 12% cows had high progesterone in both the samples suggesting that at the time of AI these cows were either in the luteal phase or had luteal cyst. Remaining 37% cow had intermediate progesterone concentrations on both the occasions suggesting that they were having either developing or regressing or malfunctioning corpora lutea or partial luteal cyst. Perusal of observations on progesterone concentrations showed that 18% of inseminations were done during luteal phase and another 30% were done at some inappropriate time of heat, thus suggesting that high proportion (48%) of cows under field conditions were inseminated while not in proper heat.
Narinder Singh, Gurjot Kaur Mavi, Ajeet Kumar, and V S Malik
ACS Publisher
The study was planned to evaluate effect of two different superstimulation protocols (2 Day vs. 3 Day) on in vivo maturation of oocytes in buffaloes. Buffaloes (n=8) were superstimulated twice using 2x2 design at an interval of 30-35 days using 200 mg and 300 mg FSH divided into four and six tapering doses (each dose of 50 mg) given over 2 and 3 days at 12 hourly intervals. Prostaglandin F2α analogue (500 μg Cloprostenol; i/m) at 72 and 84 hrs after start of superstimulatory treatment. Oocytes were retrieved from > 6 mm follicles at 48 h after the last FSH injection by ovum pick up. Oocyte maturation was assessed by the degree of cumulus cell expansion and by Orcein staining of denuded oocytes. OPU of 60 and 91 follicles resulted in recovery of 38 and 50 cumulus oocyte complexes (COCs) in 2-Day and 3-Day group, respectively. Significantly higher proportion of expanded oocytes was recovered in 3-Day protocol compared to 2-Day protocol (36.0% vs. 13.2%) and higher number of compact COCs were recovered in 2-Day protocol. In 3-Day protocol, significantly higher percentage of oocytes retrieved was at Metaphase-II stage compared to 2-Day protocol (40% vs.15.8%, respectively). The preliminary trial demonstrated that 3-day superstimulatory protocol yielded higher percentage of in vivo matured oocytes than 2-Day protocol in buffaloes.
Narinder Singh, Prahlad Singh, Khushpreet Singh, and Ajeet Kumar
ACS Publisher
The study was conducted to evaluate the efficacy of Lugol’s iodine (Intrauterine) and gentamicin (parenteral and post-AI) in repeat breeding cows (n=54). Repeat breeding cows were divided into two groups based upon the type of cervico-vaginal mucus (CVM) discharge, Group-1, n=28, repeat breeding cows having pus flakes or pus in uterine discharge; Group-2, n=26, repeat breeding cows having apparently clear CVM. For treatment purpose, 14 cows from Group-1 as Group 1(a) were infused 50 ml of 0.5% lugol solution once on day of estrus and 14 cows as Group 1(b) were injected gentamicin @ 5 mg per kg body weight intra muscular twice daily for three days, starting on the day of estrus. In the Group 2(a), repeat breeding cows (n=15) were infused gentamicin @ 2 mg body weight twenty hours post AI. Group 2(b) comprising of 11 cows served as untreated control. Overall conception rate in Group 1(a) was 72.7% as compared with 60% in Group 1(b) with 1.8 and 2.1 services per conception. Conception rate in repeat breeding cows having apparently normal CVM treated with post AI gentamicin intrauterine infusion didn’t improve as compared to control (40 vs. 36). It was concluded that the intra uterine infusion of lugol’s solution once on day of estrus in repeat breeding cows having purulent vaginal discharge was better in cleaning uterus of uterine infections than parenteral administration of gentamicin for three days
Sunita Kumari Meena, Ajeet Kumar, and Anil K. Singh
Elsevier
Prahlad Singh, Deepak Sharma, Sumit Singhal, Ajeet Kumar, Ashwani Kumar Singh, and Mrigank Honparkhe
Elsevier BV
Prahlad Singh, Munish Kumar Bedi, Sumit Singhal, Ashwani Kumar Singh, Ajeet Kumar, and Mrigank Honparkhe
Elsevier BV
Suresh K. Pothireddy, Mrigank Honparkhe, Ankit Kumar Ahuja, Shahbaz Singh Dhindsa, and Prahlad Singh
ACS Publisher
The study evaluated the fertility response following Estradoublesynch and progesterone-based Ovsynch protocols in delayed pubertal buffalo heifers. A total of twenty delayed pubertal buffalo heifers were subdivided in two equal subgroups. Group I animals were treated with PGF2α analogue (500 µg cloprostenol sodium) i.m. on day 0, GnRH analogue (20 µg buserelin acetate) on day 2, PGF2α analogue (500 µg cloprostenol sodium) repeated on day 9 and estradiol benzoate 1 mg intramuscular (i.m.) on day 10. Fixed-time artificial insemination (FTAI) was done at 48 and 60h. Group II animals were subjected to GnRH analogue (20 µg buserelin acetate) i.m. along with CIDR insert intravaginally on day 0, PGF2α analogue (500 µg cloprostenol sodium) i.m. and removal of CIDR on day 7, GnRH analogue on day 9 followed by FTAI at 12 and 24h. Blood collection (plasma P4) and ultrasonography (luteal and follicular dynamics) was performed on day 0, 9, 10, 12 in group I and day 0, 7, 9 and 10 in group II animals. Pregnancy diagnosis was made at 45-60 days post AI. Estrus response was observed in all the buffalo heifers of group I and II. First service conception rate was significantly higher in group I (50%) compared to group II (20%) (P<0.05). Overall pregnancy rate was almost similar in both group I and II (60% vs 70%). In conclusion, estradoublesynch and progesterone-based ovsynch protocol can effectively induce ovulatory estrus and improve pregnancy rates in delayed pubertal buffalo heifers.
Ajeet Kumar, Shiv kumar, Amarjeet Bisla, Ashwani Kumar Singh, Mrigank Honparkhe, and Shahbaz Singh Dhindsa
ACS Publisher
The present experiment was conducted to study the impact of herbal treatment on post thaw semen quality and fertility in subfertile buffalo bulls. Three Murrah subfertile buffalo bulls maintained at bull station, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Ludhiana, India (Latitude/Longitude, 30.55°N, 75.54° E) with the history of poor semen quality (pre-freeze individual motility < 60%, post-thaw individual motility < 40%, Viability < 70% and Abnormality > 20%) were orally supplemented with herbal mixture (Panax ginseng roots, Shilajit, Withania somnifera roots, Tribulus terrestris fruits, Turnera diffusa leaves, Ptychopetalum olacoides bark of each 400 mg/100 kg body weight and 300 mg/100 kg body weight of Pausinystalia yohimbe bark) daily for 60 days of treatment phase. Two semen ejaculates per week per bull were collected during pre-treatment, treatment and post-treatment phases of 60 days each and extended with Tris egg yolk extender. The freezing of extended semen was carried out in a biofreezer (4°C to -15°C @-30°C/min, -15°C to -140°C @-50°C/min) followed by plunging into liquid nitrogen for storage. Post thaw semen quality was assessed in terms of individual motility, viability, total sperm abnormalities. Present study revealed that the oral treatment of subfertile buffalo bulls with Panax ginseng roots, Shilajit, Withania somnifera roots, Tribulus terrestris fruits, Turnera diffusa leaves; Ptychopetalum olacoides bark each @ 400 mg/100 kg body weight and Pausinystalia yohimbe bark @ 300 mg/100 kg body weight for 60 days improved post thaw semen quality and fertility.
Amarjeet Bisla, Nakul Gulia, Mrigank Honparkhe, and Ajeet Kumar
Exploratory Animal and Medical Research, West Bengal Veterinary Alumni Association
Paramveer Singh Sangha, Ajeet Kumar, Mrigank Honparkhe, Jasbir Singh Bedi, Ashwani Kumar, and Prahlad Singh
Agricultural Research Communication Center
Background: Pesticides act as endocrine disruptors and cause enormous disturbances in steroidogenesis, spermatogenesis and sexual behavioural display. These toxicants modulate and/or disrupt the reproductive and hormonal environment by acting on hypothalamus, pituitary and reproductive organs. Few studies have been carried out to assess the levels of pesticides in dairy cattle / buffaloes. The information on pesticide residues in body fluids especially blood and semen in breeding bulls is lacking. Method: In the light of above background nineteen fresh semen ejaculates were collected from Milkfed, Khanna and PLDB (Punjab Livestock Development Board), Nabha, Punjab and stored at -80◦C. Immediately after collection, the semen was centrifuged at 4500 × g for 20 min at 4◦C. The supernatant (seminal plasma) was stored at -20◦C until processing for the assessment of pesticide residues using gas chromatography (GC). In the present study on crossbred breeding bulls, the semen samples were analyzed for organochlorines, organophosphates and synthetic pyrethroid pesticide residues. Seven OCP were screened namely Heptachlor epoxide, Chlordane, Fipronil, Lindane, Methoxychlor, op-DDT, Endrin. Eleven OPP were screened namely Chorpyrifos, Dichlorovos, Ethion, Monocrotophos, Malathion, Parathion-methyl, Profenphos, Phorate, Triazophos, Quinalphos and Phosalone and four SP’s namely Cypermethrin, Permethrin, Deltamethrin, Cyalothrin were screened in the semen samples. Semen samples were also assessed for viability, HOST (%) and abnormality (%) for both fresh as well as post thaw samples. Result: None of the semen samples were found positive for any of the above pesticide residues. This may be attributed to the feeding of fodder cultivated at the stations without using any pesticides, hence no pesticide residue found in the semen samples of the crossbred breeding bulls of these semen stations In present study, viability (%) of sperms were found to be significantly higher (p less than 0.05) in fresh (75.57 ± 5.31) as compared to post thaw (70.17 ± 4.64) semen of bulls. The abnormality (%) of sperms was found to be significantly lower (p less than 0.05) in fresh (13.61 ± 3.71) than post thaw (18.22 ± 3.59) semen bulls. Also in present study the host (%) was found 67.23±4.76 in fresh semen which decreased to 46.82±3.55 in post thaw semen.
Rakesh Kumar, Kaushalendra Kumar, Ajeet Kumar, Sanjay Kumar, Pankaj Kumar Singh, Ravi Ranjan Kumar Sinha, and Chandra Moni
Springer Science and Business Media LLC
AshwaniKumar Singh, Sukhjinder Kaur, Mrigank Honparkhe, Ajeet Kumar, Prahlad Singh, and Udeybir Singh
Medknow
Objective: To assess the effect of flaxseed supplementation on metabolic profile, endocrine concentrations, non-esterified fatty acids (NEFA), body composition variables, and reproductive performance of sows. Methods: All the 21 crossbred Large White Yorkshire sows were considered in the study period starting at day 1 of current farrowing when the feeding of specific supplemental ration was started until the day of subsequent farrowing (days 150-155) and were equally allocated into three groups. Group 1 served as the control group and followed their normal feeding schedule. Group 2 and group 3, in addition to their normal feeding schedule, were supplemented with flaxseed at a rate of 0.5% and 1.0% of the dry matter, respectively. Blood samples were collected 15 days prior to farrowing, on the day of farrowing (day 0), at weekly intervals until day 28 of lactation and at monthly intervals during gestation to harvest the plasma. Plasma was used to assess the metabolic and endocrine status of sows. Body weight of each sow and individual birth weight of all piglets born were measured. Results: Flaxseed supplementation led to decrease in plasma cholesterol and triglyceride levels in the supplemented groups than in the control group (P<0.05). Plasma estradiol-17β level was higher in group 2 than that in group 1 and 3 on day 90 of the gestation period (P<0.05). The mean plasma level of insulin-like growth factor 1 was higher in group 3 than that in group 1 and 2 both in late lactation (day 28) as well as in early gestation (day 30) (P<0.05). Plasma NEFA and weight gain were greater in sows of group 2 and 3 compared to those fed with the normal control diets (P<0.05). The proportion of pregnant sows relative to sows bred was 100.0% in group 2 and 3 and 85.7% in the control group. Piglet mortality was lower in group 2 and 3 compared to group 1 (P>0.05). Conclusions: Flaxseed improves endocrine profiles, NEFA concentrations and body weight, resulting in better pregnancy rate and litter size.