Melissa L Knothe Tate

Scopus Publications

Cell Biophysics–Physiological Contexts, from Organism to Cell, In Vivo to In Silico Models: One Collaboratory’s Perspective
Melissa L. Knothe Tate, Sara McBride-Gagyi, Eric J. Anderson, Lucy Ngo
Biophysica, 2026
Here we present a retrospective, integrative review of the approaches and discoveries of our “collaboratory”, a meta-laboratory comprising cross-disciplinary collaborations across laboratories at fourteen different universities and clinics in seven different countries with shared lead investigators. By tying together insights from four decades of research and discovery, applied across cell types, as well as different tissues, organ systems, and organisms, we have aimed to elucidate the interplay between organisms’ movement and the physiology of their tissues, organs, and organ systems’ resident cells. We highlight the potential of increasing imaging and computing power, as well as machine learning/artificial intelligence approaches, to delineate the Laws of Biology. Codifying these laws will provide a foundation for the future, to promote not only the discovery of underpinning mechanisms but also the sustainability of our natural resources, from our brains to our bones, which serve as veritable “hard drives”, physically rendering a lifetime of cellular experiences and millennia of evolution.
Rivers of Tears – Convergent, Multi-Scale Approaches to Monitor and Optimize the Health of Our World's Inhabitants
Eric J. Anderson, Vittorio Sansalone, Luke P. Lee, Rebecca Grainger, Sohie Lee, Albert L. Juhasz, Melissa L. Knothe Tate
Global Challenges, 2026
The connectivity and interdependence of our world and its inhabitants’ health have come under increasing focus. Elucidation of the common and interdependent mechanisms of health and disease requires approaches that facilitate understanding of complex systems behavior and probing of both individual and collective system parameters. To this end, multiscale physical and computational modeling offers a particularly powerful tool to predict behavior over vast time and length scales. Other novel technologies, e.g., rapid isolation nanotechnology developed to analyze nanoscale small extracellular vesicles in ocular tears, enable tracking of “fingerprints” from diseases as diverse as ocular to neurodegenerative (e.g., dementia) and cancer. In the future, it will be possible to track the health and disease of ecosystems and their inhabitants, using geospatial and epidemiological approaches, as well as novel biotechnologies, to prevent and mitigate disease processes and enhance well‐being. These concepts are applied by way of an exemplary approach to understand and address the impact of toxic, recalcitrant manmade chemicals (i.e., PFAS) on the health of ecosystems and their diverse inhabitants. Such convergent efforts will be necessary and a priority for solving the complex problems threatening the health of our planet and its inhabitants.
Stem cell mechanoadaptation. II. Microtubule stabilization and substrate compliance effects on cytoskeletal remodeling
Vina D. L. Putra, Kristopher A. Kilian, Melissa L. Knothe Tate
APL Bioengineering, 2025
Stem cells adapt to their local mechanical environment by rearranging their cytoskeleton, which underpins the evolution of their shape and fate as well as the emergence of tissue structure and function. Here, in the second part of a two-part experimental series, we aimed to elucidate spatiotemporal cytoskeletal remodeling and resulting changes in morphology and mechanical properties of cells and their nuclei. Akin to mechanical testing of the most basic living and adapting unit of life, i.e., the cell, in situ in model tissue templates, we probed native and microtubule-stabilized (via Paclitaxel, PAX, exposure) stem cells' cytoskeletal adaptation capacity on substrates of increasing compliance (exerting local tension on cells) and with increased target seeding densities (exerting local compression on cells). On 10 and 100 kPa gels, cells seeded at 5000 cells/cm2 and cells proliferated to 15 000 cells/cm2 exhibited bulk moduli that nearly matched those of their respective substrates; hence, they exhibited a greater increase in Young's Modulus after microtubule stabilization than cells cultured on glass. Culture on compliant substrates also reduced microtubule-stabilized cells' F-actin, and microtubule concentration increases compared to cells seeded on glass. On gels, F-actin alignment decreased as more randomly oriented, short actin crosslinks were observed, representing emergent adaptation to the compliant substrate, mediated through myosin II contractility. We conclude that stem cell adaptation to compliant substrates facilitates the accommodation of larger loads from the PAX-stabilized polymerizing microtubule, which, in turn, exerts a larger effect in determining cells' capacity to stiffen and remodel the cytoskeleton. Taken as a whole, these studies establish correlations between cytoskeleton and physical and mechanical parameters of stem cells. Hence, the studies progress our understanding of the dynamic cytoskeleton as well as shape changes in cells and their nuclei, culminating in emergent tissue development and healing.
Stem cell mechanoadaptation. I. Effect of microtubule stabilization and volume changing stresses on cytoskeletal remodeling
Vina D. L. Putra, Kristopher A. Kilian, Melissa L. Knothe Tate
APL Bioengineering, 2025
Here, we report on the first part of a two-part experimental series to elucidate spatiotemporal cytoskeletal remodeling, which underpins the evolution of stem cell shape and fate, and the emergence of tissue structure and function. In Part I of these studies, we first develop protocols to stabilize microtubules exogenously using paclitaxel (PAX) in a standardized model murine embryonic stem cell line (C3H/10T1/2) to maximize comparability with previously published studies. We then probe native and microtubule-stabilized stem cells' capacity to adapt to volume changing stresses effected by seeding at increasing cell densities, which emulates local compression and tissue template formation during development. Within the concentration range of 1–100 nM, microtubule-stabilized stem cells maintain viability and reduce proliferation. PAX stabilization of microtubules is associated with increased cell volume as well as flattening of the cell and nucleus. Compared to control cells, microtubule-stabilized cells exhibit thick, bundled microtubules and highly aligned, thicker and longer F-actin fibers, corresponding to an increase in the Young's modulus of the cell. Both F-actin and microtubule concentration increase with increasing PAX concentration, whereby the increase in F-actin is more prominent in the basal region of the cell. The corresponding increase in microtubule is observed more globally across the apical and basal region of the cell. Seeding at increasing target densities induces local compression on cells. This increase in local compression modulates cell volume and concomitant increases in F-actin and microtubule concentration to a greater degree than microtubule stabilization via PAX. Cells seeded at high density exhibit higher bulk modulus than corresponding cells seeded at low density. These data demonstrate the capacity of stem cells to adapt to an interplay of mechanical and chemical cues, i.e., respective compression and exogenous microtubule stabilization; the resulting cytoskeletal remodeling manifests as evolution of mechanical properties relevant to development of multicellular tissue constructs.
Interface tissues of the mesoderm: Periosteum, ligament, interosseous membrane, & myofascial tissues, an inspiration for next generation medical textiles
Sotiria Anastopolous, Lucy Ngo, Joanna Ng, Vina Putra, Melissa L. Knothe Tate
Current Opinion in Biomedical Engineering, 2024
A spike in circulating cytokines TNF-α and TGF-β alters barrier function between vascular and musculoskeletal tissues
Lucy Ngo, Melissa L. Knothe Tate
Scientific Reports, 2023
Molecular transport between the circulatory and musculoskeletal systems regulates articular joint physiology in health and disease. Osteoarthritis (OA) is a degenerative joint disease linked to systemic and local inflammation. Inflammatory events involve cytokines, which are secreted by cells of the immune system and modulate molecular transport across tissue interfaces (referred to as tight junction [TJ] barrier function). In a previous study from our group, OA knee joint tissues were shown to exhibit size separation of different sized molecules delivered as a single bolus to the heart (Ngo et al. in Sci. Rep. 8:10254, 2018). Here, in a follow up study of parallel design, we test the hypothesis that two common cytokines, with multifaceted roles in the etiology of osteoarthritis as well as immune state in general, modulate the barrier function properties of joint tissue interfaces. Specifically, we probe the effect of an acute cytokine increase (spike) on molecular transport within tissues and across tissue interfaces of the circulatory and musculoskeletal systems. A single bolus of fluorescent-tagged 70 kDa dextran, was delivered intracardially, either alone, or with either the pro-inflammatory cytokine TNF-α or the anti-inflammatory cytokine TGF-β, to skeletally mature (11 to 13-month-old) guinea pigs (Dunkin-Hartley, a spontaneous OA animal model). After five minutes' circulation, whole knee joints were serial sectioned and fluorescent block face cryo-imaged at near-single-cell resolution. The 70 kDa fluorescent-tagged tracer is analogous in size to albumin, the most prevalent blood transporter protein, and quantification of tracer fluorescence intensity gave a measure of tracer concentration. Within five minutes, a spike (acute doubling) in circulating cytokines TNF-α or TGF-β significantly disrupted barrier function between the circulatory and musculoskeletal systems, with barrier function essentially abrogated in the TNF-α group. In the entire volume of the joint (including all tissue compartments and the bounding musculature), tracer concentration was significantly decreased in the TGF-β- and TNF-α- compared to the control-group. These studies implicate inflammatory cytokines as gatekeepers for molecular passage within and between tissue compartments of our joints and may open new means to delay the onset and mitigate the progression of degenerative joint diseases such as OA, using pharmaceutical and/or physical measures.
Biomechanical, biophysical and biochemical modulators of cytoskeletal remodelling and emergent stem cell lineage commitment
Vina D. L. Putra, Kristopher A. Kilian, Melissa L. Knothe Tate
Communications Biology, 2023
Across complex, multi-time and -length scale biological systems, redundancy confers robustness and resilience, enabling adaptation and increasing survival under dynamic environmental conditions; this review addresses ubiquitous effects of cytoskeletal remodelling, triggered by biomechanical, biophysical and biochemical cues, on stem cell mechanoadaptation and emergent lineage commitment. The cytoskeleton provides an adaptive structural scaffold to the cell, regulating the emergence of stem cell structure-function relationships during tissue neogenesis, both in prenatal development as well as postnatal healing. Identification and mapping of the mechanical cues conducive to cytoskeletal remodelling and cell adaptation may help to establish environmental contexts that can be used prospectively as translational design specifications to target tissue neogenesis for regenerative medicine. In this review, we summarize findings on cytoskeletal remodelling in the context of tissue neogenesis during early development and postnatal healing, and its relevance in guiding lineage commitment for targeted tissue regeneration. We highlight how cytoskeleton-targeting chemical agents modulate stem cell differentiation and govern responses to mechanical cues in stem cells’ emerging form and function. We further review methods for spatiotemporal visualization and measurement of cytoskeletal remodelling, as well as its effects on the mechanical properties of cells, as a function of adaptation. Research in these areas may facilitate translation of stem cells’ own healing potential and improve the design of materials, therapies, and devices for regenerative medicine.
Paclitaxel release from hollow PMMA nanoparticles: Factors affecting release rate as quantified via dialysis and membrane centrifugation
Theatin van Leeuwen, Rhiannon P. Kuchel, Melissa L. Knothe Tate, Per B. Zetterlund
Colloids and Surfaces A Physicochemical and Engineering Aspects, 2023
Despite known limitations, dialysis and centrifugation remain standard methods for quantifying in vitro drug release kinetics. In the context of quantifying drug release from nanoparticles, dialysis uses a membrane to retain nanoparticles whilst drug released from the nanoparticles transfers across the membrane. The present work investigates the use of dialysis and solely for comparison membrane centrifugation for quantitative characterization of Paclitaxel (PAX) release from hollow poly(methyl methacrylate) (PMMA) nanoparticles (PAX-NPs) synthesised using a simple one-pot miniemulsion polymerization system. The impact on drug release rate when using GSH to cleave disulfide crosslinked PMMA, the incorporation of methacrylic acid as a co-monomer, and use of a hydrotrope (sodium subsalicylate) for enhanced drug solubility are discussed. Finally, we summarise the limitations of dialysis and membrane centrifugation due to a complex interplay of factors including drug concentration, molecular weight, hydrophobicity, internal to external dialysis volume and partitioning.
Connectomics of Bone to Brain—Probing Physical Renderings of Cellular Experience
Melissa L. Knothe Tate, Abhilash Srikantha, Christian Wojek, Dirk Zeidler
Frontiers in Physiology, 2021
“Brainless” cells, the living constituents inhabiting all biological materials, exhibit remarkably smart, i.e., stimuli-responsive and adaptive, behavior. The emergent spatial and temporal patterns of adaptation, observed as changes in cellular connectivity and tissue remodeling by cells, underpin neuroplasticity, muscle memory, immunological imprinting, and sentience itself, in diverse physiological systems from brain to bone. Connectomics addresses the direct connectivity of cells and cells’ adaptation to dynamic environments through manufacture of extracellular matrix, forming tissues and architectures comprising interacting organs and systems of organisms. There is imperative to understand the physical renderings of cellular experience throughout life, from the time of emergence, to growth, adaptation and aging-associated degeneration of tissues. Here we address this need through development of technological approaches that incorporate cross length scale (nm to m) structural data, acquired via multibeam scanning electron microscopy, with machine learning and information transfer using network modeling approaches. This pilot case study uses cutting edge imaging methods for nano- to meso-scale study of cellular inhabitants within human hip tissue resected during the normal course of hip replacement surgery. We discuss the technical approach and workflow and identify the resulting opportunities as well as pitfalls to avoid, delineating a path for cellular connectomics studies in diverse tissue/organ environments and their interactions within organisms and across species. Finally, we discuss the implications of the outlined approach for neuromechanics and the control of physical behavior and neuromuscular training.
Osteoarthritis: New Strategies for Transport and Drug Delivery across Length Scales
Lucy Ngo, Melissa L. Knothe Tate
ACS Biomaterials Science and Engineering, 2020
Osteoarthritis (OA) is the fourth leading cause of disability in adults. Yet, few viable pharmaceutical options exist for pain abatement and joint restoration, aside from joint replacement at late and irreversible stages of the disease. From the first onset of OA, as joint pain increases, individuals with arthritis increasingly reach for drug delivery solutions, from taking oral glycosaminoglycans (GAGs) bought over the counter from retail stores (e.g., Costco) to getting injections of viscous, GAG-containing synovial fluid supplement in the doctor's office. Little is known regarding the efficacy of delivery mode and/or treatment by such disease-modulating agents. This Review addresses the interplay of mechanics and biology on drug delivery to affected joints, which has profound implications for molecular transport in joint health and (patho)physiology. Multiscale systems biology approaches lend themselves to understand the relationship between the cell and joint health in OA and other joint (patho)physiologies. This Review first describes OA-related structural and functional changes in the context of the multilength scale anatomy of articular joints. It then summarizes and categorizes, by size and charge, published molecular transport studies, considering changes in permeability induced through inflammatory pathways. Finally, pharmacological interventions for OA are outlined in the context of molecular weights and modes of drug delivery. Taken together, the current state-of-the-art points to a need for new drug delivery strategies that harness systems-based interactions underpinning molecular transport and maintenance of joint structure and function at multiple length scales from molecular agents to cells, tissues, and tissue compartments which together make up articular joints. Cutting edge and cross-length and -time scale imaging represents a key discovery enabling technology in this process.
Advanced Design and Manufacture of Mechanoactive Materials Inspired by Skin, Bones, and Skin-on-Bones
Melissa Louise Knothe Tate
Frontiers in Bioengineering and Biotechnology, 2020
In vitro biocompatibility and biomechanics study of novel, Microscopy Aided Designed and ManufacturEd (MADAME) materials emulating natural tissue weaves and their intrinsic gradients
Joanna L. Ng, Vina D.L. Putra, Melissa L. Knothe Tate
Journal of the Mechanical Behavior of Biomedical Materials, 2020
Mechanomics Approaches to Understand Cell Behavior in Context of Tissue Neogenesis, During Prenatal Development and Postnatal Healing
Vina D. L. Putra, Min Jae Song, Sarah McBride-Gagyi, Hana Chang, Kate Poole, Renee Whan, David Dean, Vittorio Sansalone, Melissa L. Knothe Tate
Frontiers in Cell and Developmental Biology, 2020
Mapping the Mechanome–A Protocol for Simultaneous Live Imaging and Quantitative Analysis of Cell Mechanoadaptation and Ingression
Vina Putra, Iman Jalilian, Madeline Campbell, Kate Poole, Renee Whan, Florence Tomasetig, Melissa Knothe Tate
Bio Protocol, 2019
Biotextilogy - Prototyping and testing mechanical gradient textiles that emulate Nature's own
Joanna L. Ng, Tegtaranjit Singh, Lawrence C. Kwan, Melissa L. Knothe Tate
Results in Materials, 2019
Electron Microscopy Sample Preparation Protocol Enabling Nano-to-mesoscopic Mapping of Cellular Connectomes and Their Habitats in Human Tissues and Organs
Lucy Ngo, Anton Nathanson, Tomasz Garbowski, Ulf Knothe, Dirk Zeidler, Melissa Knothe Tate
Bio Protocol, 2019
Comprehensive pressure profiling to develop next-generation compression treatment for lymphedema: Testing efficacy of high resolution sensors
Daniel J. Hageman, Shuying Wu, Daniel Latimer, Karen Virdi, Chun H. Wang, Melissa L. Knothe Tate
Sensors and Actuators A Physical, 2019
Knee joint tissues effectively separate mixed sized molecules delivered in a single bolus to the heart
Lucy Ngo, Lillian E. Knothe, Melissa L. Knothe Tate
Scientific Reports, 2018
Biotechnologies toward Mitigating, Curing, and Ultimately Preventing Edema through Compression Therapy
Daniel J. Hageman, Shuying Wu, Sharon Kilbreath, Stanley G. Rockson, Chun Wang, Melissa L. Knothe Tate
Trends in Biotechnology, 2018
Prospective design, rapid prototyping, and testing of smart dressings, drug delivery patches, and replacement body parts using Microscopy Aided Design and ManufacturE (MADAME)
Hans Jörg Sidler, Jacob Duvenage, Eric J. Anderson, Joanna Ng, Daniel J. Hageman, Melissa L. Knothe Tate
Frontiers in Medicine, 2018
Engineering mechanical gradients in next generation biomaterials – Lessons learned from medical textile design
Joanna L. Ng, Ciara E. Collins, Melissa L. Knothe Tate
Acta Biomaterialia, 2017
Establishing the basis for mechanobiology-based physical therapy protocols to potentiate cellular healing and tissue regeneration
Joanna L. Ng, Mariana E. Kersh, Sharon Kilbreath, M. Knothe Tate
Frontiers in Physiology, 2017
Scale-up of nature's tissue weaving algorithms to engineer advanced functional materials
Joanna L. Ng, Lillian E. Knothe, Renee M. Whan, Ulf Knothe, Melissa L. Knothe Tate
Scientific Reports, 2017
Live tissue imaging to elucidate mechanical modulation of stem cell niche quiescence
Nicole Y.C. Yu, Connor A. O’Brien, Iveta Slapetova, Renee M. Whan, Melissa L. Knothe Tate
Stem Cells Translational Medicine, 2017
Translating periosteum’s regenerative power: Insights from quantitative analysis of tissue genesis with a periosteum substitute implant
Shannon R. Moore, Céline Heu, Nicole Y.C. Yu, Renee M. Whan, Ulf R. Knothe, Stefan Milz, Melissa L. Knothe Tate
Stem Cells Translational Medicine, 2016
Creating High-Resolution Multiscale Maps of Human Tissue Using Multi-beam SEM
André F. Pereira, Daniel J. Hageman, Tomasz Garbowski, Christof Riedesel, Ulf Knothe, Dirk Zeidler, Melissa L. Knothe Tate
Plos Computational Biology, 2016
Emergence of form from function—Mechanical engineering approaches to probe the role of stem cell mechanoadaptation in sealing cell fate
Melissa L. Knothe Tate, Peter W. Gunning, Vittorio Sansalone
Bioarchitecture, 2016
The Only Constant Is Change: Next Generation Materials and Medical Device Design for Physical and Mental Health
Melissa L. Knothe Tate, Thomas Fath
Advanced Healthcare Materials, 2016
Organ-to-Cell-Scale Health Assessment Using Geographical Information System Approaches with Multibeam Scanning Electron Microscopy
Melissa L. Knothe Tate, Dirk Zeidler, André F. Pereira, Daniel Hageman, Tomasz Garbowski, Sanjay Mishra, Lauren Gardner, Ulf R. Knothe
Advanced Healthcare Materials, 2016
Engineering and commercialization of human-device interfaces, from bone to brain
Melissa L. Knothe Tate, Michael Detamore, Jeffrey R. Capadona, Andrew Woolley, Ulf Knothe
Biomaterials, 2016
Molecular transport in musculoskeletal health and disease
Roy K Aaron
Skeletal Circulation in Clinical Practice, 2016
High-resolution, high-throughput imaging with a multibeam scanning electron microscope
A.L. EBERLE, S. MIKULA, R. SCHALEK, J. LICHTMAN, M.L. KNOTHE TATE, D. ZEIDLER
Journal of Microscopy, 2015
Mapping the stem cell’s mechanome using paired live cell multiplexed imaging and modeling
Richard Oldfield, Iman Jalilian, Min Jae Song, Melissa Knothe Tate
Ifmbe Proceedings, 2015
Flow-induced stress on adherent cells in microfluidic devices
Jonathan Shemesh, Iman Jalilian, Anthony Shi, Guan Heng Yeoh, Melissa L. Knothe Tate, Majid Ebrahimi Warkiani
Lab on A Chip, 2015
Erratum: Outside back cover (volume 15, issue 21) (Lab on a Chip - Miniaturisation for Chemistry and Biology (2015) 15 (4234))
Jonathan Shemesh, Iman Jalilian, Anthony Shi, Guan Heng Yeoh, Melissa L. Knothe Tate, Majid Ebrahimi Warkiani
Lab on A Chip, 2015
Medial meniscal displacement and strain in three dimensions under compressive loads: MR assessment
Maren Freutel, Andreas M. Seitz, Fabio Galbusera, Axel Bornstedt, Volker Rasche, Melissa L. Knothe Tate, Anita Ignatius, Lutz Dürselen
Journal of Magnetic Resonance Imaging, 2014
Periosteal thickness and cellularity in mid-diaphyseal cross-sections from human femora and tibiae of aged donors
Shannon R. Moore, Stefan Milz, Melissa L. Knothe Tate
Journal of Anatomy, 2014
The linea aspera: A virtual case study testing emergence of form and function
Shannon R. Moore, Stefan Milz, Melissa L. Knothe Tate
Anatomical Record, 2014
Mechanistic, Mathematical Model to Predict the Dynamics of Tissue Genesis in Bone Defects via Mechanical Feedback and Mediation of Biochemical Factors
Shannon R. Moore, Gerald M. Saidel, Ulf Knothe, Melissa L. Knothe Tate
Plos Computational Biology, 2014
Arthritic periosteal tissue from joint replacement surgery: A novel, autologous source of stem cells
Hana Chang, Denitsa Docheva, Ulf R. Knothe, Melissa L. Knothe Tate
Stem Cells Translational Medicine, 2014
Lithotripsy stimulates new bone formation and mitigates loss of bone due to disuse in aged rats
Melissa L. Knothe Tate, Jared O'Leary, Erin McNamara, Lan Cai, Ulf R. Knothe
Technology and Health Care, 2013
Mechanical modulation of nascent stem cell lineage commitment in tissue engineering scaffolds
Min Jae Song, David Dean, Melissa L. Knothe Tate
Biomaterials, 2013
Anatomic sampling site and perfusate osmolarity effects on periosteum intrinsic permeability
S. Lemonnier, T. Lemaire, S. Naili, M.L. Knothe Tate
Computer Methods in Biomechanics and Biomedical Engineering, 2013
Elucidating multiscale periosteal mechanobiology: A key to unlocking the smart properties and regenerative capacity of the periosteum?
Sarah F. Evans, Hana Chang, Melissa L. Knothe Tate
Tissue Engineering Part B Reviews, 2013
Solid-supported lipid bilayers to drive stem cell fate and tissue architecture using periosteum derived progenitor cells
Sarah F. Evans, Denitsa Docheva, Anja Bernecker, Céline Colnot, Ralf P. Richter, Melissa L. Knothe Tate
Biomaterials, 2013
Periosteum, bone's "smart" bounding membrane, exhibits direction-dependent permeability
Sarah F Evans, Jonathan B Parent, Colin E Lasko, Xiaowen Zhen, Ulf R Knothe, Thibault Lemaire, Melissa L Knothe Tate
Journal of Bone and Mineral Research, 2013
Computational modeling of tissue engineering scaffolds as delivery devices for mechanical and mechanically modulated signals
Min Jae Song, David Dean, Melissa L. Knothe Tate
Studies in Mechanobiology Tissue Engineering and Biomaterials, 2013
Current insights on the regenerative potential of the periosteum: Molecular, cellular, and endogenous engineering approaches
Céline Colnot, Xinping Zhang, Melissa L. Knothe Tate
Journal of Orthopaedic Research, 2012
Multiscale computational and experimental approaches to elucidate bone and ligament mechanobiology using the ulna-radius-interosseous membrane construct as a model system
M.L. Knothe Tate, A.E. Tami, P. Netrebko, S. Milz, D. Docheva
Technology and Health Care, 2012
Mapping the mechanome of live stem cells using a novel method to measure local strain fields in situ at the fluid-cell interface
Min Jae Song, Susann M. Brady-Kalnay, Sara H. McBride, Polly Phillips-Mason, David Dean, Melissa L. Knothe Tate
Plos One, 2012
Concise review: The periosteum: Tapping into a reservoir of clinically useful progenitor cells
Hana Chang, Melissa L. Knothe Tate
Stem Cells Translational Medicine, 2012
Surgical membranes as directional delivery devices to generate tissue: Testing in an ovine critical sized defect model
Melissa L. Knothe Tate, Hana Chang, Shannon R. Moore, Ulf R. Knothe
Plos One, 2011
Structure - Function relationships in the stem cell's mechanical world A: Seeding protocols as a means to control shape and fate of live stem cells
Mcb Molecular and Cellular Biomechanics, 2011
Structure - Function relationships in the stem cell's mechanical world B: emergent anisotropy of the cytoskeleton correlates to volume and shape changing stress exposure
Mcb Molecular and Cellular Biomechanics, 2011
Multiscale mechanobiology of de novo bone generation, remodeling and adaptation of autograft in a common ovine femur model
Melissa L. Knothe Tate, Scott Dolejs, Sarah H. McBride, R. Matthew Miller, Ulf R. Knothe
Journal of the Mechanical Behavior of Biomedical Materials, 2011
Anisotropic mechanical properties of ovine femoral periosteum and the effects of cryopreservation
Sarah H. McBride, Sarah F. Evans, Melissa L. Knothe Tate
Journal of Biomechanics, 2011
Net change in periosteal strain during stance shift loading after surgery correlates to rapid de novo bone generation in critically sized defects
Sarah H. McBride, Scott Dolejs, Stefano Brianza, Ulf Knothe, Melissa L. Knothe Tate
Annals of Biomedical Engineering, 2011
Major and minor centroidal axes serve as objective, automatable reference points to test mechanobiological hypotheses using histomorphometry
Sarah H. McBride, Scott Dolejs, Ulf Knothe, Melissa L. Knothe Tate
Journal of Biomechanics, 2011
Top down and bottom up engineering of bone
Melissa L. Knothe Tate
Journal of Biomechanics, 2011
Smart body armor inspired by flow in bone
Melissa Louise Knothe Tate
Smart Structures and Systems, 2011
Role of mechanical loading in healing of massive bone autografts
Melissa L. Knothe Tate, Scott Dolejs, R. Matthew Miller, Ulf R. Knothe
Journal of Orthopaedic Research, 2010
Computational modeling of extravascular flow in bone
Melissa L. Knothe Tate, Roland Steck, Andrea Tami, Hans-Jörg Sidler, Eric J. Anderson, Peter Niederer
Computational Modeling in Biomechanics, 2010
In situ spatiotemporal mapping of flow fields around seeded stem cells at the subcellular length scale
Min Jae Song, David Dean, Melissa L. Knothe Tate
Plos One, 2010
Effects of mechanical loading patterns, bone graft, and proximity to periosteum on bone defect healing
Ulf R. Knothe, Scott Dolejs, R. Matthew Miller, Melissa L. Knothe Tate
Journal of Biomechanics, 2010
Molecular dynamics computations of flow in constricted and wavy nano channels
D. D. Marsh, S. P. Vanka, I. M. Jasiuk, M. L. Knothe Tate
ASME International Mechanical Engineering Congress and Exposition Proceedings, 2010
Bone as an inspiration for a novel class of mechanoactive materials
Melissa L. Knothe Tate, Roland Steck, Eric J. Anderson
Biomaterials, 2009
Modulation of stem cell shape and fate A: The role of density and seeding protocol on nucleus shape and gene expression
Sara H. McBride, Melissa L. Knothe Tate
Tissue Engineering Part A, 2008
Cell and Tissue Engineering - Taking cues from nature's engineering paradigm for developing, growing, and repairing tissues
Melissa L. Knothe Tate, Stanislav Y. Shvartsman, Avner Friedman
Tissue Engineering Part A, 2008
Modulation of stem cell shape and fate B: Mechanical modulation of cell shape and gene expression
Sara H. McBride, Thomas Falls, Melissa L. Knothe Tate
Tissue Engineering Part A, 2008
Mechanical modulation of osteochondroprogenitor cell fate
Melissa L. Knothe Tate, Thomas D. Falls, Sarah H. McBride, Radhika Atit, Ulf R. Knothe
International Journal of Biochemistry and Cell Biology, 2008
Idealization of pericellular fluid space geometry and dimension results in a profound underprediction of nano-microscale stresses imparted by fluid drag on osteocytes
Eric J. Anderson, Melissa L. Knothe Tate
Journal of Biomechanics, 2008
Pairing computational and scaled physical models to determine permeability as a measure of cellular communication in micro- and nano-scale pericellular spaces
Eric J. Anderson, Steven M. Kreuzer, Oliver Small, Melissa L. Knothe Tate
Microfluidics and Nanofluidics, 2008
Open access to novel dual flow chamber technology for in vitro cell mechanotransduction, toxicity and pharamacokinetic studies
Eric J Anderson, Melissa L Knothe Tate
Biomedical Engineering Online, 2007
Design of tissue engineering scaffolds as delivery devices for mechanical and mechanically modulated signals
Eric J. Anderson, Melissa L. Knothe Tate
Tissue Engineering, 2007
Testing of a new one-stage bone-transport surgical procedure exploiting the periosteum for the repair of long-bone defects
Melissa L. Knothe Tate, Todd F. Ritzman, Erich Schneider, Ulf R. Knothe
Journal of Bone and Joint Surgery, 2007
The imperative for controlled mechanical stresses in unraveling cellular mechanisms of mechanotransduction
Eric J Anderson, Thomas D Falls, Adam M Sorkin, Melissa L Knothe Tate
Biomedical Engineering Online, 2006
The ratio between lacunar and canalicular gap size dominates predictions of permeability in the pericellular fluid space of bone
Proceedings of the 2005 Summer Bioengineering Conference, 2005
Site-specific porosity and its impact on load-induced fluid movement in cortical bone
Proceedings of the 2005 Summer Bioengineering Conference, 2005
Performance evaluation of tissue engineering scaffolds - Development of a novel tool for optimization of fluid flow & permeability
Proceedings of the 2005 Summer Bioengineering Conference, 2005
Erratum: 'Culture shock' from the bone cell's perspective: Emulating physiological conditions for mechanobiological investigations (American Journal of Physiology - Cell Physiology (December 2004) 287 (C1527-C1536) DOI: 10.1152/ajpcell.00059.2004
American Journal of Physiology Cell Physiology, 2005
Preface: Special issue on bone fluid flow: Organ to cell, lab bench to bedside, on earth and in space
Melissa L. Knothe Tate
Annals of Biomedical Engineering, 2005
In silico stochastic network models that emulate the molecular sieving characteristics of bone
Roland Steck, Melissa L. Knothe Tate
Annals of Biomedical Engineering, 2005
Erratum: A novel ex vivo model for investigation of fluid displacements in bone after endoprosthesis implantation (Journal of Materials Science: Materials in Medicine 10:12 (801-806))
Editorial Board
Journal of Materials Science Materials in Medicine, 2005
Application of stochastic network models for the study of molecular transport processes in bone
Roland Steck, Melissa L. Knothe Tate
Advances in Bioengineering BED, 2004
"Culture shock" from the bone cell's perspective: Emulating physiological conditions for mechanobiological investigations
Adam M. Sorkin, Kay C. Dee, Melissa L. Knothe Tate
American Journal of Physiology Cell Physiology, 2004
Modeling the effects of interstitial fluid flow on a single osteocyte and its processes
Eric J. Anderson, Sathya Kaliyamoorthy, Melissa L. Knothe Tate
Advances in Bioengineering BED, 2004
Permeability of cortical bone to large molecular weight proteins under diffusive and load-induced convective transport mechanisms
R. B. Patel, A. E. Tami, M. L. Knothe Tate
Advances in Bioengineering BED, 2004
Performance evaluation of four cell flow chambers: How well is stress controlled at a cellular level?
Eric J. Anderson, Adam Sorkin, Melissa L. Knothe Tate
Advances in Bioengineering BED, 2004
The osteocyte
Melissa L Knothe Tate, Josée R Adamson, Andrea E Tami, Thomas W Bauer
International Journal of Biochemistry and Cell Biology, 2004
Effect of interosseous membrane on load transfer in rat forelimb using finite element analysis
A. E. Tami, G. Suresh, R. B. Patel, M. L. Knothe Tate
Advances in Bioengineering BED, 2004
Induction of microdamage through application of acoustic energy to cortical bone
Andrea E. Tami, Melissa L. Knothe Tate, Jamie R. Streem, Ryan S. Comisford, Jared M. O’Leary, Stevan B. Streem, Ulf Knothe
Advances in Bioengineering BED, 2004
Probing the tissue to subcellular level structure underlying bone's molecular sieving function
Biorheology, 2003
"Whither flows the fluid in bone?" An osteocyte's perspective
Melissa L Knothe Tate
Journal of Biomechanics, 2003
Effect of lacunocanalicular architecture on hydraulic conductance in bone tissue: Implications for bone health and evolution
Sanjay Mishra, Melissa L. Knothe Tate
Anatomical Record Part A Discoveries in Molecular Cellular and Evolutionary Biology, 2003
A finite element analysis for the prediction of load-induced fluid flow and mechanochemical transduction in bone
R. STECK, P. NIEDERER, M.L. KNOTHE TATE
Journal of Theoretical Biology, 2003
Noninvasive fatigue fracture model of the rat ulna
A. E. Tami, P. Nasser, M. B. Schaffler, M. L. Knothe Tate
Journal of Orthopaedic Research, 2003
Measurement of bone surface strains on the sheep metacarpus in vivo and ex vivo
R. Steck, C. Gatzka, E. Schneider, P. Niederer, M. L. Tate
Veterinary and Comparative Orthopaedics and Traumatology, 2003
The role of interstitial fluid flow in the remodeling response to fatigue loading
A. E. Tami, P. Nasser, O. Verborgt, M. B. Schaffler, M. L. Knothe Tate
Journal of Bone and Mineral Research, 2002
Development of preparation methods for and insights obtained from atomic force microscopy of fluid spaces in cortical bone
Helmut F. Knapp, Andreas Stemmer, Gwendolen C. Reilly, Peter Niederer, Melissa L. Knothe Tate
Scanning, 2002
Cellular and tissue-level permeability are increased in fractured and in contralateral control bones
American Society of Mechanical Engineers Bioengineering Division Publication BED, 2001
The role of interstitial fluid flow in the remodeling response to fatigue and disuse
American Society of Mechanical Engineers Bioengineering Division Publication BED, 2001
Effect of loading waveform and strain rate on interstitial fluid flow and mass transport in bone
American Society of Mechanical Engineers Bioengineering Division Publication BED, 2001
Investigation of the morphology of the lacunocanalicular system of cortical bone using atomic force microscopy
Gwendolen C. Reilly, Helmut F. Knapp, Andreas Stemmer, Peter Niederer, Melissa L. Knothe Tate
Annals of Biomedical Engineering, 2001
Mixing mechanisms and net solute transport in bone
Melissa L. Knothe Tate
Annals of Biomedical Engineering, 2001
Effect of anisotropic material properties on interstitial fluid flow patterns in mechanically loaded bone
Journal of Biomechanics, 2001
The influence of mechanical stimulus on the pattern of tissue differentiation in a long bone fracture - An FEM study
T.N. Gardnera, T. Stoll, L. Marks, S. Mishra, M. Knothe Tate
Journal of Biomechanics, 2000
A finite difference model of load-induced fluid displacements within bone under mechanical loading
R Steck, P Niederer, M.L Knothe Tate
Medical Engineering and Physics, 2000
An ex vivo model to study transport processes and fluid flow in loaded bone
M.L. Knothe Tate, U. Knothe
Journal of Biomechanics, 2000
300 years of intramedullary fixation - From Aztec practice to standard treatment modality
Ulf Knothe, Melissa L. Knothe Tate, Stephan M. Perren
European Journal of Trauma, 2000
Development and testing of a new self-locking intramedullary nail system: Testing of handling aspects and mechanical properties
Ulf Knothe, Melissa L Knothe Tate, Kaj Klaue, Stephan M Perren
Injury, 2000
In vivo demonstration of load-induced fluid flow in the rat tibia and its potential implications for processes associated with functional adaptation
M.L. Knothe Tate, R. Steck, M. R. Forwood, P. Niederer
Journal of Experimental Biology, 2000
Role of mechanical load-induced fluid flow in osteocyte nutrition and its clinical implications
Annual International Conference of the IEEE Engineering in Medicine and Biology Proceedings, 1999
A novel ex vivo model for investigation of fluid displacements in bone after endoprosthesis implantation
C. Gatzka, E. Schneider, M. L. Knothe Tate, U. Knothe, P. Niederer
Journal of Materials Science Materials in Medicine, 1999
A theoretical FE-based model developed to predict the relative contribution of convective and diffusive transport mechanisms for the maintenance of local equilibria within cortical bone
American Society of Mechanical Engineers Bioengineering Division Publication BED, 1998
In vivo tracer transport through the lacunocanalicular system of rat bone in an environment devoid of mechanical loading
M.L. Knothe Tate, P. Niederer, U. Knothe
Bone, 1998
A THEORETICAL FE-BASED MODEL DEVELOPED TO PREDICT THE RELATIVE CONTRIBUTION OF CONVECTIVE AND DIFFUSIVE TRANSPORT MECHANISMS FOR THE MAINTENANCE OF LOCAL EQUILIBRIA WITHIN CORTICAL BONE
Melissa L. Knothe Tate, Peter Niederer
ASME International Mechanical Engineering Congress and Exposition Proceedings Imece, 1998
Experimental elucidation of mechanical load-induced fluid flow and its potential role in bone metabolism and functional adaptation
Melissa L. Knothe Tate, Ulf Knothe, Peter Niederer
American Journal of the Medical Sciences, 1998
Konvektiver Transport als möglicher Mechanismus bei Knochenumbauprozessen
M. L. Knothe Tate, P. Niederer, S. Tepic
Biomedizinische Technik, 1996

Melissa L Knothe Tate

Scopus Publications

RECENT SCHOLAR PUBLICATIONS

MOST CITED SCHOLAR PUBLICATIONS