Dr. Ramanlal Narayan Kachave
@Sapkal Knowledge Hub, Kalyanii Hills, Anjaneri-Wad, 422213, Nasik, India
Professor, Department of Pharmaceutical Chemistry
R .G. Sapkal College of Pharmacy, Anjaneri, Nashik, Affiliated Savitribai Phule Pune University India
Brief Profile: 16 years of Professional experience in academics and served at different level as Lecturer, Assistant Professor, PG and Ph. D Guide etc and Currently Serving as the Assocaite Professor at R.G. Sapkal College of Pharmacy, Nasik. Completed a PhD in Pharmaceutical Sciences from the JNTU, Kakinada (AP) and M. Pharmacy in Pharmaceutical Chemistry from School of Pharmaceutical Sciencs, RGPV, Bhopal (M.P). Received Savitribai Phule Pune University, Pune’s BCUD Two minor research project grant of Rs 3.0 lakhs. Qualified GATE examination Conducated by IIT, Mumabi 2005.
EDUCATION
M. Pharmacy , Pharmaceutical Chemistry
Ph. D. , Pharmaceutical Science
RESEARCH INTERESTS
Analytical and Bioanalytical Method Development and Validation
Impurity Profiling Study
Stability Indicating Assay Methods
FUTURE PROJECTS
Bioanalytical Method Development and Validation of Antihyperuricemic Drug in human Urine Sample by Liquid Chromatography-Mass Spectrometry
Applications Invited
Scopus Publications
Scopus Publications
Asmita Gaikwad, Nandu Kayande, Harshal Tare, Babaso Udugade, and Ramanlal Kachave
Dr. Yashwant Research Labs Pvt. Ltd.
This research focuses on the in-silico design of multi-target agents for acute myeloid leukemia (AML), targeting GSK-3β and VEGFR2. Using TTD data, we selected a promising target pair and considered 20,818 agents. Ligand-based screening in ChEMBL identified compounds with diverse structures and favorable interactions. Protein structures (GSK-3β: PDB ID 1Q5K, VEGFR2: PDB ID 3QTK) underwent rigorous quality assessment, indicating high-quality models. Molecular docking revealed varied affinities, with CHEMBL183504 showing strong affinity for GSK-3β in pocket C1, and CHEMBL185922 for VEGFR2 in pocket 2. CHEMBL181959 exhibited dual affinity. Further experimental validation is needed. In conclusion, this study provides insights for AML therapy, guiding compound selection and structural quality assessment for potential drug development
Harshal Tare, Anjali Bedse, Ujjwala Thube, Ramanlal Kachave, and Vijay Wagh
Dr. Yashwant Research Labs Pvt. Ltd.
Understanding binding interactions between flavanones and human epidermal growth factor receptor 2 (HER2) is an important step in developing effective treatments for breast cancer, and this study applies computational methods to do just that. This research presents a comprehensive computational methodology for identifying potential HER2 inhibitors with a focus on breast cancer treatment. The study leverages a combination of structural and pharmacophore-based approaches, starting with bioactive compound selection from the ChEMBL 2D database. The PDB-REDO refined crystal structure of Kinase domain of Human HER2 (erbB2) was used to conduct molecular docking simulations with the identified drugs. The Kleywegt-like plot analysis demonstrates the improved structural quality of the HER2 kinase domain after refinement, showing enhanced agreement with experimental data. Molecular docking simulations, conducted using the AutoDock tool, reveal the binding affinity and interaction patterns of selected compounds with the HER2 receptor. Virtual screening results highlight compounds with high binding affinity, favorable interaction patterns, and structural compatibility as potential lead candidates. To ensure safety and efficacy, ADMETox filtering was employed, providing insights into the compound’s toxicity profile and pharmacokinetic attributes. The selected compound, Eriodictyol (C20H20O6), exhibits a generally favorable safety profile, with predicted inactivity across multiple toxicity classifications and endpoints. While immunotoxicity is predicted, the overall low probabilities suggest a relatively low risk. Physicochemical and pharmacokinetic assessments indicate Eriodictyol’s potential for drug development. With a molecular weight of 356.37 g/mol, balanced lipophilicity, and high gastrointestinal absorption, the compound aligns with drug-likeness criteria. However, careful consideration is warranted due to its inhibitory effects on certain enzymes and alerts for catechol_A and isolated_alkene. In conclusion, this integrated computational approach streamlines the identification of potential HER2 inhibitors, offering a systematic strategy for drug discovery. Eriodictyol emerges as a promising candidate, demonstrating a favorable safety profile and pharmacokinetic attributes, paving the way for further in-depth studies and development as a potential therapeutic agent for breast cancer.
Harshal Tare, Ujjwala Thube, Ramanlal Kachave, Vijay Wagh, and Babaso Udugade
Dr. Yashwant Research Labs Pvt. Ltd.
This research explores the potential of carefully selected catechins as catalase modulators, leveraging their documented antioxidant effects. The catalase protein’s structural quality was rigorously evaluated, revealing an overall high-quality 3D structure. Ligand-based virtual screening identified ten novel catechin hits with promising interactions, presenting candidates designed for new validation. Molecular docking simulations demonstrated robust binding empathies between selected catechins and catalase, with CHEMBL223855 exhibiting the highest affinity. ADMET analysis highlighted CHEMBL223855 as a promising candidate for drug development, boasting favorable properties, including high gastrointestinal absorption and absence of blood-brain barrier permeation. Despite medicinal chemistry alerts and lead likeness violations, this comprehensive analysis guides future experimental validation efforts, supporting the potential of these catechins as effective catalase modulators.
Vijay Wakale, Ramanlal Kachave, Pranjal Gholap, Kiran Mahajan, and Harshal Tare
Dr. Yashwant Research Labs Pvt. Ltd.
This research employs a comprehensive approach to investigate potential therapeutic candidates for lung adenocarcinoma through drug-drug transcriptomic similarity analysis and molecular docking simulations. Using the Connectivity Map Touchstone tool, we identified compounds with high transcriptomic similarity to genistein, revealing potential shared mechanisms of action. The selected compounds, including avrainvillamide-analog-2, were further assessed through molecular docking simulations against the tyrosine kinase inhibitor (TKI) enzyme. Avrainvillamide-analog-2 exhibited a remarkable binding affinity in pocket C2, interacting with key amino acids. The results provide valuable insights into the pharmacological properties of the identified compounds, laying the groundwork for future experimental validations and drug development initiatives. Additionally, cavities detection by CB Dock server and structural refinement by PDB REDO contribute to the overall understanding of ligand binding and protein structure. This integrative approach offers a holistic perspective for identifying potential lead compounds and understanding their molecular interactions, facilitating the rational design of novel therapeutics for lung adenocarcinoma.
Sumit Deore, Ramanlal Kachave, Pranjal Gholap, Kiran Mahajan, and Harshal Tare
Dr. Yashwant Research Labs Pvt. Ltd.
This study employs a hybrid computational approach to identify potential methionyl-tRNA synthetase inhibitors for Brucella melitensis. Utilizing ligand-based pharmacophore screening and structure-based blind docking, we selected a lead compound, CHEMBL349379, from the ChEMBL 2D database. Docking simulations revealed high binding affinity and favorable interactions. Lead optimization using ADMETlab 2.0 demonstrated promising drug-like properties, but a detailed toxicity analysis highlighted concerns. Experimental validation is needed to confirm inhibitory potential and address toxicity issues. This approach streamlines the identification of potential therapeutic agents for B. melitensis treatment.
Pramod Burakale, Suresh Sudke, Manish Bhise, Harshal Tare, and Ramanlal Kachave
Dr. Yashwant Research Labs Pvt. Ltd.
The present investigation was planned to confirm the film-forming potential of synthesized rosin esters. The free films of rosin esters were prepared by solvent evaporation and characterized for the physicochemical and mechanical attributes. Suitable concentrations of rosin esters were used for coating tablets, and coated tablets were evaluated for official and unofficial quality control tests. The free films had low tensile strength, higher percent elongation value, and smooth surfaces. Therefore, the plasticizer was used to provide tensile strength to the films. The tablets were coated rapidly without agglomeration, confirming the suitability of rosin esters as a coating agent. The drug release from tablets was delayed up to 6 to 8 hours because of 10% w/w coating of rosin ester, and regioselectivity was achieved by coating with hydroxypropyl methylcellulose with sodium bicarbonate. The pH-dependent solubility of rosin esters produces chrono-triggered drug release from coated tablets. The present investigation confirms the suitability of newly synthesized rosin esters in in designing a region selective chrono- triggered drug delivery system.
Anjali Bedse, Ashwini Nalawade, Suchita Dhamane, Ramanlal Kachave, Vijay Wagh, Harshal Tare, and Gauri Ghangale
Dr. Yashwant Research Labs Pvt. Ltd.
The most frequent reason patients seek medical assistance is because of a persistent cough, despite the fact that coughing is both a crucial defensive reflex and a universal indication of health. According to an epidemiologic study, up to 40% of people report coughing. Upper respiratory tract infections (URTIs) and the common cold are the most frequent causes of cough, but other causes include post-infectious cough, undiagnosed chronic cough, and cough brought on by pulmonary diseases like asthma, chronic obstructive pulmonary disease (COPD), idiopathic pulmonary fibrosis, and lung cancer. The most common causes of cough in children are viral URTI, chronic bacterial bronchitis, and asthma. Whether acute or chronic, cough is linked to considerably reducing health-related quality of life. Patients with chronic cough commonly report sleep disturbance, nausea, chest pains, lethargy, social humiliation, urine incontinence, and low mood. Coughing may not be effective in certain circumstances (such as respiratory tract inflammation, neoplasia, eosinophilic bronchitis, airway irritation from various pollutants, airway hyperresponsiveness from infection, gastroesophageal reflux disease, and coughing without any known cause, also known as idiopathic cough). Opioidergic central cough suppressants, such as opioids, codeine, pholcodine, noscapine, and dextromethorphan, are useful when coughing is ineffective. Constipation, sedation, respiratory depression, dependence, drowsiness, addiction, and even mortality can result from prolonged use of these cough suppressants, which restricts their use in people. The proposed research project’s objective is to develop and assess herbal dosage forms that contain the widely used spice Piper longum L. (Piperaceae).
Sweta Rai, Raj K. Keservani, Prashant Kumar, Vikrant Kisanrao Nikam, Ramanlal N. Kachave, Yatindra Kumar, and Rajesh K. Kesharwani
Elsevier
Kajal P. Baviskar and Ramanlal N. Kachave
Bentham Science Publishers Ltd.
Abstract: Sartans are often used as antihypertensives. They are also available in combination with thiazide diuretics for the management of hypertension. Analytical method development is a crucial part of successful drug development and characterization. Bioanalytical studies are of paramount importance while establishing pharmacokinetic and toxicokinetic data while forced degradation studies are important to elucidate degradation pathways and to establish stability of the drugs. : Different methods have been developed for the analysis of sartans and their combination with thiazide diuretics. We thought it imperative to summarize them so the data could be useful for analysis of newer sartans. The review describes various methods for analysis of some frequently employed sartans as well as the latest sartans and their combination with thiazide diuretics. The article also focuses on their analysis of biological fluids. Forced degradation studies have also been covered in the article. : Article is divided into three sections. First section covers introduction, second section focuses on different methods developed, including bioanalytical methods, while third section presents forced degradation studies carried out on the drugs. Important parameters of the analytical methods developed have been summarized in tabular form.
Sadhana Pawar, Kajal Pawade, Sonali Nipate, Aishawarya Balap, Bhushan Pimple, Vijay Wagh, and Ramanlal Kachave
International Journal of Experimental Research and Review
Human body has several multi-layered organs, but skin is one of biggest and easiest to access. It serves as body's primary line of defense alongside various skin diseased conditions. Despite receiving sufficient and appropriate care, diabetes wounds heal slowly and may take a week to complete. A progression of connective tissue patch up is the body's natural defense against tissue damage. Fresh leaves of Ficus racemosa were utilized for the study. In this study, two distinct models were employed to compare how well different Ficus racemosa leaf extracts healed wounds. Excision wounds healed more quickly and to a greater extent after being treated with a flavonoid and tannin fraction of Ficus racemosa leaf extract, suggesting improved epithelization. The extract-treated groups also experienced an increase in breaking potency of incision wounds made; higher breaking strength indicates better wound healing. Complete closure of wound of flavonoid fraction and in fraction of Ficus racemosa extract occurred in 16 and 17 days respectively. Standard treatment increased tensile strength in the diabetic linear incision wound model, followed by treatment with the flavonoid fraction and tannin fraction of Ficus racemosa leaves extract. Ultimate finding and outcome of the present study experimentally demonstrates that extracts of the flavonoid and tannin fractions of Ficus racemosa have wound-healing properties and are effective in treating diabetic wounds. From this study, we state that Ficus racemosa flavonoid fraction and tannin fraction extract has a beneficial effect on blood glucose levels, which shows hypoglycaemic activity.
Ramanlal N. Kachave, Reshma N. Shelke, and Manoj V. Mahale
Bentham Science Publishers Ltd.
Abstract: Epilepsy is one of the chronic diseases seen in 1.0% of the world’s population. For the study of antiepileptic medications, a variety of approaches have been used extensively. These methods provide reliable, accurate and reproducible results. The available analytical methods for the determination of AEDs in API, biological fluids and pharmaceutical formulations are reviewed in this context. The drugs categorized under AEDs were studied for differentanalytical procedures, methods and systematically classified on the basis of their applications. The analytical instruments used for determination of AEDs include various chromatographic techniques such as High-performance liquid chromatography (HPLC), Ultra performance liquid chromatography (UPLC), Gas chromatography (GC) etc. and other techniques such as hyphenated, spectrophotometric, electrochemical analysis including UV, MS/MS, ECD, CLND, Fluorescence, colometric electrochemical detector (CED) are covered in this review. Supporting electrolyte, pH, panel, mobile phase, measuring or detection potential, sensitivity, and selectivity are all recorded as part of the analytical method formulation and validation process. This review is beneficial for various researchers for further study and advancement in research related to antiepileptic drugs.
Vaishnavi A. Sarangdhar and Ramanlal N. Kachave
Pleiades Publishing Ltd
R. N. Kachave and A. G. Mundhe
Walter de Gruyter GmbH
Abstract Aim Nebivolol and valsartan are used in the treatment of hypertension. So, this study was conducted for the purpose of determining bioavailability/bioequivalence of nebivolol and valsartan in human plasma. Materials and Methods The chromatographic separation was performed on Symmetry C18 (150 × 4.6 mm, 5 μm) column using 0.01 N potassium dihydrogen phosphate (pH 3.0):acetonitrile (60:40) as the mobile phase at a flow rate of 1.0 mL/min and a detector wavelength of 280 nm. The retention times of nebivolol and valsartan in plasma were found to be 3.1 and 4.3 min, respectively. Results The method was validated statistically and by recovery studies. The linearity concentration was acceptable in the range of 0.5–10 ng/mL for nebivolol and 400–8000 ng/mL for valsartan. The lower limits of quantification were 0.5 ng/mL for nebivolol and 400 ng/mL for valsartan, which reached the levels of both drugs possibly found in human plasma. Per cent recoveries were obtained as 97.78% and 98.11% for nebivolol and valsartan, respectively. Conclusion The proposed method is simple, rapid, accurate, precise and gives us knowledge about the pharmacokinetics and therapeutic drug monitoring in clinical laboratories.
RamanlalN Kachave, PragatiB Mandlik, and SnehalR Wakchaure
Medknow
The aim of this study was to provide information of the development in analytical perspective of impurity profiling, force degradation, and bioanalysis of pharmaceutical drug substance and drug products used for the treatment of gout. This information was discussed on the basis of year of publication, matrix (active pharmaceutical ingredient, dosage form, and biological fluid), sample preparation technique, column and types of elution in chromatography (isocratic or gradient), detector, and therapeutic categories of drug, which were used for analysis. It focuses mainly on analytical methods including hyphenated techniques for the identification and quantification of impurity, degradants, and metabolites in different pharmaceutical and biological matrices.
Megha N. Salunkhe, Snehal D. Gite, and Ramanlal N. Kachave
Informa UK Limited
ABSTRACT This review describes the recent trends in analytical perspectives of impurity profiling and forced degradation of pharmaceutical drug and drug products used for the treatment of hypertension. Hypertension classes include diuretics, angiotensin receptor blocker, β adrenergic blocker, calcium channel blocker. Description based on use of various chromatographic methods, stationary phase, column, detectors are mentioned. Recent advancement in the field of impurity and stability study are focused on drugs belonging to these categories. GRAPHICAL ABSTRACT
Ramanlal Kachave, Mayura Kale, and Rajendra Wagh
Modestum Publishing Ltd
A new high performance liquid chromatography method was developed and validated for the quantitation of Cilnidipine and Valsartan in pharmaceutical formulations. Determination was performed using an ODS C18, 250mm x 4.6mm, 5μm column, a mobile phase containing Methanol: Water (85:15) pH 3 adjusts with ortho-phosphoric acid in isocratic flow rate 1.0 mLmin-1. The method was validated with respect to linearity, precision, robustness, and accuracy. The calibration graphs ranged from 1-5 μg/mL in Cilnidipine and 8-40 μg/mL Valsartan Intraand interday relative standard deviation values for the standard solutions were 0.5%, 1.64% and 0.22%, 1.62 %. Robustness of relative standard deviation values was 0.334, 0.101 respectively. Total recoveries of Cilnidipine and Valsartan from the laboratory prepared mixtures were 98.94 % and 99.04 % respectively.
Deepti Jain, Raman N. Kachave, and Rajendra N. Bhadane
Informa UK Limited
A new high performance liquid chromatography method was developed and validated for the quantitation of tramadol, paracetamol, and domperidone in pharmaceutical formulations. Determination was performed using a Inertsil-ODS-3 (C-18) column (5 μm, 250 mm × 4.60 mm), a mobile phase containing methanol–phosphate buffer (pH 4.0; 40 + 60, v/v), in gradient flow rate 1.2 mL. The method was validated with respect to linearity, precision, robustness, and accuracy. The calibration graphs ranged from 250 to 1500 mg/mL for paracetamol, 25 to 150 mg/mL for tramadol, and 5 to 30 mg/mL for domperidone. Intra- and interday relative standard deviation values for the standard solutions were 0.077%, 0.98%, and 1.04%, respectively. Repeatability of relative standard deviation values was 0.115%, 0.494%, and 1.97% respectively. Total recoveries of paracetamol, tramadol, and domperidone from the laboratory prepared mixtures were 100.4, 100.06, and 100.2%, respectively.
Piyush Trivedi, C. Kartikeyan, Raman Kachave, and Rajendra Bhadane
Informa UK Limited
Abstract A novel stability indicating high performance liquid chromatographic assay method was developed and validated for Olmesartan medoxomil and its degradant product. An isocratic HPLC method was developed to separate the drug from the degradation products, using an Inertsil-ODS-3 (C-18) Column (5 µm, 250 mm × 4.60 mm). A mixture of phosphate buffer (pH 4.0) and methanol (30:70) was used as mobile phase. The flow rate was 1.0 mL/min and the detection was carried out at 230 nm. The validation studies were carried out fulfilling the International Conference on Harmonisation (ICH) requirements. The procedure was found to be specific, linear, precise (including intra and inter day precision), accurate, and robust.
RECENT SCHOLAR PUBLICATIONS
Publications
1. Ramanlal N. Kachave et al. Impurity Profiling and Forced Degradation Study of Angiotensin (AT1) Blockers: A Review. Analytical Science Springer. Communicated (
2. Ramanlal N. Kachave et al. Bioanalytical Method Development and Validation of Isosorbide Mononitrate in human plasma by using LC-MS/MS. Journal of Analytical Chemistry. Springer. Communicated (
3. Ramanlal N. Kachave et al. Development and Validation of Stability-Indicating RP-HPLC method for Azilsartan Medoxomil. Beni-Suef University Journal of Basic and Applied Sciences Springer. Communicated
4. Ramanlal N. Kachave et al. Overview of UHPLC-MS: An effective and sensitive hyphenated technique. Journal of Analytical Chemistry Springer Nature. Accepted (
5. Ramanlal N. Kachave et al. A Review: Recent Analytical Applications on Anti-Epileptic Agents. Current Pharmaceutical Analysis, Bentham Sciences. Accepted (
6. Ramanlal N. Kachave et al. Bioanalytical method development and validation of cilnidipine and metoprolol succinate by RP-HPLC: Its pharmacokinetics application. Current Chinese Chemistry, Bentham Science, 2(1), 2022, 59-64. DOI: 10.2174/22106766116662106142035.
7. Ramanlal N. Kachave et al. Stability indicating assay method for determination of Febuxostat by RP-HPLC. Current Chinese Chemistry, Bentham Science 2(1), 2022, 50-58. DOI:10.2174/2666001601666210427114547.
8. Ramanlal N. Kachave et al. Bioanalytical Method Development and Validation of Nebivolol and Valsart
GRANT DETAILS
MODROB Research Grant from AICTE, New Delhi
Year 2019-2022 (,
Successfully receiving the research grants from BCUD Savitribai Phule Pune University, Pune
Year 2016-18 (Rs.1, 40,000/-)
Year 2011-13 (Rs.1, 60,000/-)