Bioactive ZnO Decorated PVDF-Based Piezoelectric, Osteoconductive Nanofibrous Coatings for Orthopedic Implants Sumedh Vaidya, Mansi Joshi, Sumanta Ghosh, Namdev More, Ravichandiran Velyutham, Srivalliputtur Sarath Babu, Govinda Kapusetti Journal of Biomedical Materials Research Part A, 2025 Surface modification of titanium‐based orthopedic implants has been investigated over the last decades to promote better bone‐to‐implant association, osseointegration, and fracture healing. Yet, post‐surgical failure of coated orthopedic implants occurs due to poor adhesive strength, fatigue failure, high wear rate of coated materials, low biocompatibility, limited osseointegration, and stress‐shielding effect. Therefore, there is an unmet clinical need to develop a smart coating strategy. Herein, we have created an electrospun nanofibrous coating for Ti‐implants using piezoelectric Polyvinylidene fluoride (PVDF) polymer reinforced with osteoconductive nanofiller Zinc oxide (ZnO). We have found that by varying the ZnO content from 0.5 to 2.0 wt.% in the PVDF matrix, we can modulate the electrospun coating's mechanical, thermal, physicochemical stability, and piezoelectric characteristics. Our results proved that PVDF‐ZnO nanofibrous coatings exhibit almost ~3–4 fold increase in the piezoelectric d33 coefficient as well as output voltage, compared to pure PVDF using Piezo‐responsive Force Microscopy (PFM). Furthermore, electrically poled piezoelectric PVDF‐ZnO nanofibers also demonstrated a significant increment (~5‐fold) in collagen deposition, hydroxyapatite formation, and improved bio‐ and hemo‐compatibility compared to unpoled nanofibers. Furthermore, through the in vitro experiments, we have confirmed that the piezoelectric PVDF‐ZnO nanofibrous activates calcium‐calmodulin mediated cellular pathway to induce cell adhesion, proliferation, and cell spreading in the osteoblast cells. Nonetheless, using the biomimetic mechanical bioreactor, we have investigated the piezoelectricity‐mediated increased focal adhesion and enhanced F‐actin production under the physiologically relevant (i.e., 1%) mechanical strain in bone cells. Moreover, the current study elucidates the piezoelectric‐based smart, multifunctional coating strategies for developing an osteoconductive implant.
Injectable PLGA Microscaffolds with Laser-Induced Enhanced Microporosity for Nucleus Pulposus Cell Delivery P. Nakielski, Alicja Kosik-Kozioł, Chiara Rinoldi, Daniel Rybak, Namdev More, Jacob Wechsler, Tomasz P Lehmann, Maciej Głowacki, Bogusz Stępak, Magdalena Rzepna, Martina Marinelli, Massimiliano Lanzi, D. Seliktar, Sarah Mohyeddinipour, Dmitriy Sheyn, F. Pierini Small, 2025 Intervertebral disc (IVD) degeneration is a leading cause of lower back pain (LBP). Current treatments primarily address symptoms without halting the degenerative process. Cell transplantation offers a promising approach for early-stage IVD degeneration, but challenges such as cell viability, retention, and harsh host environments limit its efficacy. This study aimed to compare the injectability and biocompatibility of human nucleus pulposus cells (hNPC) attached to two types of microscaffolds designed for minimally invasive delivery to IVD. Microscaffolds are developed from poly(lactic-co-glycolic acid) (PLGA) using electrospinning and femtosecond laser structuration. These microscaffolds are tested for their physical properties, injectability, and biocompatibility. This study evaluates cell adhesion, proliferation, and survival in vitro and ex vivo within a hydrogel-based nucleus pulposus model. The microscaffolds demonstrate enhanced surface architecture, facilitating cell adhesion and proliferation. Laser structuration improved porosity, supporting cell attachment and extracellular matrix deposition. Injectability tests show that microscaffolds can be delivered through small-gauge needles with minimal force, maintaining high cell viability. The findings suggest that laser-structured PLGA microscaffolds are viable for minimally invasive cell delivery. These microscaffolds enhance cell viability and retention, offering potential improvements in the therapeutic efficiency of cell-based treatments for discogenic LBP.
ECM-mimetic, NSAIDs loaded thermo-responsive, immunomodulatory hydrogel for rheumatoid arthritis treatment Dipesh Kumar Shah, Sumanta Ghosh, Namdev More, Mounika Choppadandi, Mukty Sinha, Sarath Babu Srivalliputtur, Ravichandiran Velayutham, Govinda Kapusetti BMC Biotechnology, 2024 Background Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease, and it leads to irreversible inflammation in intra-articular joints. Current treatment approaches for RA include non-steroidal anti-inflammatory drugs (NSAIDs), disease-modifying anti-rheumatic drugs (DMARDs), corticosteroids, and biological agents. To overcome the drug-associated toxicity of conventional therapy and transdermal tissue barrier, an injectable NSAID-loaded hydrogel system was developed and explored its efficacy. Results The surface morphology and porosity of the hydrogels indicate that they mimic the natural ECM, which is greatly beneficial for tissue healing. Further, NSAIDs, i.e., diclofenac sodium, were loaded into the hydrogel, and the in vitro drug release pattern was found to be burst release for 24 h and subsequently sustainable release of 50% drug up to 10 days. The DPPH assay revealed that the hydrogels have good radical scavenging activity. The biocompatibility study carried out by MTT assay proved good biocompatibility and anti-inflammatory activity of the hydrogels was carried out by gene expression study in RAW 264.7 cells, which indicate the downregulation of several key inflammatory genes such as COX-2, TNF-α & 18s. Conclusion In summary, the proposed ECM-mimetic, thermo-sensitive in situ hydrogels may be utilized for intra-articular inflammation modulation and can be beneficial by reducing the frequency of medication and providing optimum lubrication at intra-articular joints. Graphical Abstract
The role of Piezo1 and Piezo2 proteins in tissue engineering: A Comprehensive review Tejaswini Tadge, Ashwini Pattewar, Namdev More, Srivalliputtur Sarath Babu, Ravichandiran Velyutham, Govinda Kapusetti Engineered Regeneration, 2024 Almost every life form, from the tiniest bacterium to humans, is mechanosensitive, implying it can use mechanical stresses to trigger certain physiological responses in the form of electric signals. Mechanotransduction largely relies on ion channels that respond to mechanical forces, such as the epithelial sodium channels/degenerins, transient receptor potential channel, and the two-pore domain potassium channel. Piezo1 and Piezo2 proteins were discovered to be the biggest non-selective mechanosensitive cation channels in the cell membrane. A substantial amount of research has previously been published on the Piezo channel's function in touch sensation, balance, and cardiovascular regression. However, the mechanistic perspective must be refined to fully understand the role of Piezo proteins in tissue engineering. This review centers on the latest insights into the structure of Piezo channels, activation mechanisms, and its interactions with cytoskeletal components, by emphasizing the physiological activities of Piezo channels in different tissues. The study also places focus on the possibilities of targeting this cation channel family as a tissue regeneration aid.
Polymeric Nanocarriers for Drug Delivery to the Central Nervous System Aditya Kadam, Shradha Karande, Shreya Thakkar, Namdev More, Dilip Sharma Nanoarchitectonics for Brain Drug Delivery, 2024 The central nervous system (CNS) presents unique challenges in drug delivery. The blood–brain barrier plays a very disturbing role in drug delivery to the brain. Recent studies have emerged with a nanoparticle approach that continuously functions to improve delivery to the brain. Polymer nanoparticles represent a promising solution to these problems. Currently, polymer nanoparticles are widely involved in many therapies for spatial and temporal delivery to target areas. Consequently, nanoparticles are capable of delivering a wide range of drugs into the brain, including anti-cancer medications, analgesics, Alzheimer’s medications, and protease inhibitors. Additionally, nanoparticles can help reduce drug toxicity and side effects by changing their distribution in the body. Biocompatible and rapidly biodegradable nanoparticles are important for delivering nanoparticles into the brain. Therefore, macromolecule-derived nanocarriers, such as polysaccharides and proteins, hold the potential for the delivery of drugs. Nanocarriers derived from natural polymers are attractive for the delivery of drugs and genes to the brain due to their low cytotoxicity, abundant surface functional groups, high drug-binding capacity, and significant uptake into target cells.. Here we focus on key advances in the development of polymer nanoparticles for drug delivery to the CNS. Nanoparticles are described in terms of aspects, benefits, and limitations of their formulations, as well as the main results of in vitro and in vivo studies.
A comprehensive review on carbon quantum dots as an effective photosensitizer and drug delivery system for cancer treatment Kondi Soumya, Namdev More, Mounika Choppadandi, D.A. Aishwarya, Gajendar Singh, Govinda Kapusetti Biomedical Technology, 2023 Carbon quantum dots (CQDs) are an emerging class of carbon-based nanomaterials. CQDs are receiving considerable interest in biomedical applications due to their low toxicity, stability profile, efficient surface tailorability, aqueous solubility, diverse functionality, biocompatibility, and size-tunable emission when compared to other conventional carbon quantum dots (CDs) of group II, III, IV & V. Improved photodynamic treatment, photosensitization, bioimaging, targeted drug delivery, fluorescent marker for disease-detection, cell tracking, and biosensors are made possible by the presence of diverse functional groups on the surface of CQDs, such as thiol, carboxyl, hydroxyl, etc. This article gives thorough understanding of CQDs for drug delivery applications by describing their origin and rationale. In addition, the in-vivo characteristics of CQDs are also examined in an initiative to strengthen the argument for CQDs as powerful drug delivery vehicles.
Brinzolamide microsponge-loaded in situ gel for sustained ocular delivery: design, characterization and in vivo evaluation B Agarwal, S Chandapure, A Kakad, N More, P Panzade, P Rathi, P Patil, ... Naunyn-Schmiedeberg's Archives of Pharmacology, 1-18 , 2026 2026
A Review Exploring the Translational Perspective of Artificial Intelligence in Drug Discovery and Formulation Development B Agarwal, S Gaware, N More, R Shinde, HN Shivakumar, S Jagdale Annales Pharmaceutiques Françaises , 2026 2026 Citations: 1
Modulating Inflammation in Post-Traumatic Osteoarthritis using iPSC-derived Anti-inflammatory Macrophages N Mahmoudi, L Zila, J Sheyn, N More, M Chavez, D Roell, R LevGur, ... bioRxiv, 2026.05. 18.726078 , 2026 2026
Bioactive ZnO Decorated PVDF‐Based Piezoelectric, Osteoconductive Nanofibrous Coatings for Orthopedic Implants S Vaidya, M Joshi, S Ghosh, N More, R Velyutham, SS Babu, G Kapusetti Journal of Biomedical Materials Research Part A 113 (8), e37971 , 2025 2025 Citations: 4
iPSC-derived Anti-inflammatory Macrophages as a Preventative Treatment for Knee Post-Traumatic Osteoarthritis N Mahmoudi, J Sheyn, L Zila, A Prasad, M Chavez, N More, W Tawackoli, ... Osteoarthritis and Cartilage 33, S201-S202 , 2025 2025
Injectable PLGA Microscaffolds with Laser‐Induced Enhanced Microporosity for Nucleus Pulposus Cell Delivery P Nakielski, A Kosik‐Kozioł, C Rinoldi, D Rybak, N More, J Wechsler, ... Small 21 (16), 2404963 , 2025 2025 Citations: 4
Bioresorbable materials in controlled and smart drug delivery systems A Kadam, D Sheyn, N Maheshwari, N More Bioresorbable Materials and Bioactive Surface Coatings, 415-442 , 2025 2025
The role of Piezo1 and Piezo2 proteins in tissue engineering: a comprehensive review T Tadge, A Pattewar, N More, SS Babu, R Velyutham, G Kapusetti Engineered Regeneration 5 (2), 170-185 , 2024 2024 Citations: 24
ECM-mimetic, NSAIDs loaded thermo-responsive, immunomodulatory hydrogel for rheumatoid arthritis treatment DK Shah, S Ghosh, N More, M Choppadandi, M Sinha, SB Srivalliputtur, ... BMC biotechnology 24 (1), 26 , 2024 2024 Citations: 18
Polymeric nanocarriers for drug delivery to the central nervous system A Kadam, S Karande, S Thakkar, N More, D Sharma Nanoarchitectonics for Brain Drug Delivery, 122-147 , 2024 2024 Citations: 1
A comprehensive review on carbon quantum dots as an effective photosensitizer and drug delivery system for cancer treatment K Soumya, N More, M Choppadandi, DA Aishwarya, G Singh, G Kapusetti Biomedical Technology 4, 11-20 , 2023 2023 Citations: 143
Piezoelectric-based bioactive zinc oxide-cellulose acetate electrospun mats for efficient wound healing: an in vitro insight S Ghosh, S Vaidya, N More, R Velyutham, G Kapusetti Frontiers in Immunology 14, 1245343 , 2023 2023 Citations: 23
Cellulose-based bioabsorbable and antibiotic coated surgical staple with bioinspired design for efficient wound closure S Ghosh, S Kulkarni, N More, NK Singh, R Velyutham, NR Kumar, ... International Journal of Biological Macromolecules 248, 126477 , 2023 2023 Citations: 8
Fluorescent carbon quantum dots for effective tumor diagnosis: A comprehensive review BD Latha, K Soumya, N More, C Mounika, AT Guduru, G Singh, ... Biomedical Engineering Advances 5, 100072 , 2023 2023 Citations: 50
Natural biopolymers for bone tissue engineering: a brief review S Pramanik, S Kharche, N More, D Ranglani, G Singh, G Kapusetti Engineered Regeneration 4 (2), 193-204 , 2023 2023 Citations: 94
Ultrahigh sensitive graphene oxide/conducting polymer composite based biosensor for cholesterol and bilirubin detection A Kumar, GH Gupta, G Singh, N More, A Sharma, D Jawade, A Balu, ... Biosensors and Bioelectronics: X 13, 100290 , 2023 2023 Citations: 52
Surgical staples: Current state-of-the-art and future prospective S Ghosh, N More, G Kapusetti Medicine in Novel Technology and Devices 16, 100166 , 2022 2022 Citations: 40
Ultrahigh Sensitive Graphene Oxide/Conducting Polymer Composite Based Biosensor for Cholesterol and Bilirubin Detection A Kumar, N More, A Sharma, D Jawade, GH Gupta, A Balu, G Kapusetti Conducting Polymer Composite Based Biosensor for Cholesterol and Bilirubin … , 2022 2022 Citations: 1
Piezoelectric ceramics as stimulatory modulators for regenerative medicine N More, D Ranglani, AR Hiray, G Kapusetti Advanced Ceramics for Versatile Interdisciplinary Applications, 313-338 , 2022 2022 Citations: 9
Invertebrate-derived bioceramics: An effective alternative source for biomedical applications AR Hiray, N More, G Kapusetti Advanced Ceramics for Versatile Interdisciplinary Applications, 279-311 , 2022 2022 Citations: 1
MOST CITED SCHOLAR PUBLICATIONS
Piezoelectric smart biomaterials for bone and cartilage tissue engineering J Jacob, N More, K Kalia, G Kapusetti Inflammation and regeneration 38 (1), 2 , 2018 2018.0 Citations: 507
Piezoelectric material–a promising approach for bone and cartilage regeneration N More, G Kapusetti Medical hypotheses 108, 10-16 , 2017 2017.0 Citations: 155
A comprehensive review on carbon quantum dots as an effective photosensitizer and drug delivery system for cancer treatment K Soumya, N More, M Choppadandi, DA Aishwarya, G Singh, G Kapusetti Biomedical Technology 4, 11-20 , 2023 2023.0 Citations: 143
Smart Piezoelectric Nanohybrid of Poly(3-hydroxybutyrate- co -3-hydroxyvalerate) and Barium Titanate for Stimulated Cartilage Regeneration J Jacob, N More, C Mounika, P Gondaliya, K Kalia, G Kapusetti ACS Applied Bio Materials 2 (11), 4922-4931 , 2019 2019.0 Citations: 102
Natural biopolymers for bone tissue engineering: a brief review S Pramanik, S Kharche, N More, D Ranglani, G Singh, G Kapusetti Engineered Regeneration 4 (2), 193-204 , 2023 2023.0 Citations: 94
Ultrahigh sensitive graphene oxide/conducting polymer composite based biosensor for cholesterol and bilirubin detection A Kumar, GH Gupta, G Singh, N More, A Sharma, D Jawade, A Balu, ... Biosensors and Bioelectronics: X 13, 100290 , 2023 2023.0 Citations: 52
Fluorescent carbon quantum dots for effective tumor diagnosis: A comprehensive review BD Latha, K Soumya, N More, C Mounika, AT Guduru, G Singh, ... Biomedical Engineering Advances 5, 100072 , 2023 2023.0 Citations: 50
Fast dissolving electrospun polymeric films of anti-diabetic drug repaglinide: formulation and evaluation S Thakkar, N More, D Sharma, G Kapusetti, K Kalia, M Misra Drug development and industrial pharmacy 45 (12), 1921-1930 , 2019 2019.0 Citations: 46
Review on carbon nanomaterials as typical candidates for orthopaedic coatings SY Bhong, N More, M Choppadandi, G Kapusetti SN Applied Sciences 1 (1), 76 , 2019 2019.0 Citations: 43
Surgical staples: Current state-of-the-art and future prospective S Ghosh, N More, G Kapusetti Medicine in Novel Technology and Devices 16, 100166 , 2022 2022.0 Citations: 40
Graphene Oxide Reinforcement Enhances the Piezoelectric and Mechanical Properties of Poly(3-hydroxybutyrate- co -3-hydroxy valerate)-Based Nanofibrous … N More, A Srivastava, G Kapusetti ACS Applied Bio Materials 3 (10), 6823-6835 , 2020 2020.0 Citations: 32
Advances in contrast agents for contrast-enhanced magnetic resonance imaging S Khairnar, N More, C Mounika, G Kapusetti Journal of Medical Imaging and Radiation Sciences 50 (4), 575-589 , 2019 2019.0 Citations: 28
Introduction to ideal characteristics and advanced biomedical applications of biomaterials G Kapusetti, N More, M Choppadandi Biomedical engineering and its applications in healthcare 171, 204 , 2019 2019.0 Citations: 27
The role of Piezo1 and Piezo2 proteins in tissue engineering: a comprehensive review T Tadge, A Pattewar, N More, SS Babu, R Velyutham, G Kapusetti Engineered Regeneration 5 (2), 170-185 , 2024 2024.0 Citations: 24
Piezoelectric-based bioactive zinc oxide-cellulose acetate electrospun mats for efficient wound healing: an in vitro insight S Ghosh, S Vaidya, N More, R Velyutham, G Kapusetti Frontiers in Immunology 14, 1245343 , 2023 2023.0 Citations: 23
Electrospun mat of thermal‐treatment‐induced nanocomposite hydrogel of polyvinyl alcohol and cerium oxide for biomedical applications N More, D Ranglani, S Kharche, G Kapusetti Journal of Applied Polymer Science 137 (46), 49426 , 2020 2020.0 Citations: 19
ECM-mimetic, NSAIDs loaded thermo-responsive, immunomodulatory hydrogel for rheumatoid arthritis treatment DK Shah, S Ghosh, N More, M Choppadandi, M Sinha, SB Srivalliputtur, ... BMC biotechnology 24 (1), 26 , 2024 2024.0 Citations: 18
Natural fiber reinforced biodegradable staples: Novel approach for efficient wound closure PC Lalhmangaihzuali, BD Latha, N More, M Choppadandi, G Kapusetti Medical Hypotheses 126, 60-65 , 2019 2019.0 Citations: 17
Glucosamine-conjugated nanoseeds for chemo-magneto hyperthermia therapy of cancer A Patharkar, N Raval, D Kalyane, V Tambe, N Anup, N More, G Kapusetti, ... Journal of Drug Delivery Science and Technology 61, 102295 , 2021 2021.0 Citations: 14
Piezoelectric smart biomaterials for bone and cartilage tissue engineering, Inflamm. Regen. 38 (2018) 2 J Jacob, N More, K Kalia, G Kapusetti Citations: 13
