Development of a tissue-engineered oral mucosal barrier model using poly (l-lactic acid) electrospun nanofibers Andari Sarasati, Deniz Yucel, Halime Kenar, Hevi Wihadmadyatami, Neval Sevinc Ozdemir, Rahmi Anggraeni, Vasif Hasirci, Ika Dewi Ana Scientific Reports, 2026 The oral mucosa is a crucial barrier that defends against external factors and regulates tissue permeability. To advance trans-epithelial drug delivery and dental biomaterials research, a robust in vitro model of oral mucosal tissue is needed. This study develops an oral epithelial model using electrospun poly (L-lactic acid) (PLA) meshes. Two PLA formulations (10% and 12% in CHCl₃/ DMF) were optimized for nanoscale fibers production and subsequently functionalized via O₂ plasma treatment and fibronectin coating to enhance cell adhesion by increasing surface hydrophilicity, as measured by contact angle analysis. Oral epithelial cells (TR146) were cultured on these scaffolds. Adhesion and proliferation were evaluated using confocal microscopy and both MTS and PrestoBlue viability assays. The 12% PLA formulation produced fibers with an average diameter of 717 nm and pore sizes of approximately 366 nm, significantly improving cell adhesion and proliferation. Functionalization further enhanced cell attachment and spreading through increased hydrophilicity and targeted protein interactions. Confocal imaging revealed uniform cell distribution and multilayered growth that mimics native oral epithelium architecture. These findings demonstrate that fibronectin-coated 12% PLA electrospun meshes are promising scaffolds for oral epithelial tissue engineering and trans-epithelial delivery applications.
Aloe Vera, Trigona Honey, and Chitosan-Based Antibacterial Nanospray Modulates Mast Cells and Angiogenesis in Gingival Wound Healing Archadian Nuryanti, Ruslin ., Hana Shafira, Nadhira Afifah Mumtazrahmah, Nadia Nur Hanifah, Andari Sarasati Tropical Journal of Natural Product Research, 2026 Gingival wound healing is a complex biological process involving inflammatory, proliferative, and remodelling phases, all of which influence the rate and quality of tissue repair. This study aimed to evaluate the effects of a nanospray formulation containing Aloe vera extract, Trigona honey, and chitosan on bacterial inhibition, angiogenesis, and mast cell activity during gingival wound healing. An in vitro antibacterial assay against Porphyromonas gingivalis was performed according to standard procedure, followed by in vivo histological evaluation using 45 male Wistar rats divided into three groups: chitosan spray as a negative control, the nanospray formulation as the treatment group, and Aloclair® as a positive control. Gingival tissue samples were collected and analyzed on days 1, 3, 5, 7, and 14 post-treatment. The nanospray demonstrated significant antibacterial activity against P. gingivalis and significantly enhanced gingival wound healing by promoting angiogenesis and reducing mast cell counts compared to the control groups (p < 0.05). These findings indicate that the nanospray formulation effectively supports bacterial inhibition and accelerates tissue repair, suggesting its potential as a therapeutic approach for gingival wound management.
Surface functionalized calcium phosphate bioceramics for immunomodulatory biomaterials Andari SARASATI, Ika Dewi ANA, Deniz YUCEL, Halime KENAR, Hevi WIHADMADYATAMI, Neval Sevinc OZDEMIR, Rahmi ANGGRAENI, Vasif HASIRCI Dental Materials Journal, 2025 Calcium phosphate (CaP) bioceramics, including apatite (Ap)-based materials, are naturally biocompatible, but they frequently require surface functionalization to achieve optimal integration with biological systems, especially with immune cells. Surface functionalization strategies tailor CaP bioceramic nanoparticles to enhance cell adhesion, proliferation, differentiation, and overall biocompatibility. This is because functionalized surfaces interact more dynamically with immune cells, i.e., macrophages, dendritic cells, and lymphocytes, through surface receptors and signaling pathways. The dynamic interaction may activate immune cells, release cytokine, differentiate cells, and regulate inflammation. Therefore, to resolve the limitations of natural CaP bioceramics, surface functionalization is crucial. Modified bioceramics nanoparticles' surface properties ensure more effective integration with biological tissues. In addition, biomolecule immobilization on CaP bioceramic surfaces provides a versatile approach in establishing a foundation for the development of immunomodulatory biomaterials. This review provides an overview of recent biomedical research on CaP bioceramics, especially Ap-based materials, focusing on advancements in surface functionalization strategies designed to improve interactions with immune cells. It also examines the role of immobilized biomolecules in modulating immune responses, highlighting their potential for clinical applications.
Periodontopathogen Antibacterial Properties of PMMA-HA as a Dental Implant Candidate Biomaterial in vitro Prahasanti Chiquita, Rini Devijanti Ridwan, Patera Nugraha Alexander, Tansza Permata Setiana Putri, Nastiti Faradilla Ramadhani, Diah Savitri Ernawati, Raden Darmawan Setijanto, Devi Rianti, Tania Saskianti, Tania Saskianti, Ratri Maya Sitalaksmi, Shafira Kurnia Supandi, Narmada Ida Bagus, I Gusti Aju Wahju Ardani, Rahmawati Dwi, Fianza Rezkita, Sarasati Andari, Tengku Natasha Eleena Tengku Ahmad Noor Research Journal of Pharmacy and Technology, 2025 Introduction: Periodontopathogenic bacteria are Gram-negative anaerobic bacteria that often cause peri-implantitis such as Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Fusobacterium nucleatum. Objective: This study is to analyze the periodontopathogen antibacterial properties of Polymethylmethacrylate (PMMA) and Hydroxyapatite (HA) composites against the growth of F. nucleatum, P. gingivalis, and A. Actinomycetemcomitans in vitro. Materials and Methods: Inhibition zone of PMMA-HA against periodontopathogenic bacteria was carried out with the well diffusion method. Doxycycline 100 mg was used as a positive control while the treatment group were consisted of 5 groups; 1) the PMMA group, 2) PMMA-HA from balai besar keramik (BBK), 3) PMMA-HA group of nano particle size, 4) the HA-BBK group and 5) the HA-nano group. All samples from the treatment and positive control groups were placed on agar plate that has been inoculated with bacteria are incubated for 24 hours at temperature of 37ᵒC and were measured the inhibition zone diameter by using the digital caliper. The data was analysed using analysis of variance (ANOVA) and the post-hoc Tukey Honest Significant Different (HSD) test (p<0.05). Results: PMMA-HA has the ability to inhibit the growth of F. nucleatum, P. gingivalis, A. Actinomycetemcomitans in vitro. The antibacterial activity of PMMA-HA nanocomposite against F. nucleatum, P. gingivalis, and A. Actinomycetemcomitans was higher than PMMA-HA-BBK (p=0.0001; p<0.05). Conclusion: PMMA-HA nanocomposite has the ability to inhibit the growth of F. nucleatum, P. gingivalis, and A. Actinomycetemcomitans better than PMMA-HA-BBK which can be considered as a candidate for dental implant biomaterials with periodontopathogenic antibacterial property to prevent peri-implantitis.
An Immunoinformatic of Epigallocatechin-3-O-gallate as Adjuvant Therapy of Periodontitis: An in-silico Study Mohammed Aljunaid, Rini Devijаnti Ridwаn, Sidarningsih Sidarningsih, Yuliati Yuliati, Fianza Rezkita, et al. Tropical Journal of Natural Product Research, 2024 Periodontitis results in irreversible bone resorption. Epigallocatechin-3-O-gallate (EGCG) is one of the prominent compounds in green tea and is recognized for its therapeutic efficacy. EGCG supports bone formation and possesses antioxidant and anti-inflammatory properties. EGCG inhibits bone resorption by encouraging osteoclast apoptosis, preventing formation, and supporting the development of mineralized bone nodules. This study investigates the efficacy of EGCG in immunoinformatic as a potential treatment for periodontitis. The 3D chemical structures were obtained from the PubChem database. PyRx v.0.8 software was used to conduct molecular docking simulations. The results showed an inhibitory effect on the protein samples Nuclear Factor Associate T cell-1 (NFATc1), Sclerostin, Tartate Resistant Acid Phosphatase (TRAP), Receptor Activator of kappa beta and ligand (RANK-RANKL), Runt-related transcription factor2 (RUNX2), Osterix, and Osteocalcin. The docking analysis of target proteins RUNX2, Osterix, and Osteocalcin showed that EGCG exhibited the most negative binding energy, -7.0 kcal/mol, in the RUNX2 domain, potentially enhancing osteonectin activity. The findings indicate that the EGCG inhibits osteoclastic activity by binding and suppressing NFATc1, RANK-RANKL, Sclerostin, and TRAP. Consequently, EGCG substantially enhances osteogenic processes by promoting RUNX2, Osterix, and Osteocalcin in silico.
BDNF and Krox20 as Indicators of Platelet-rich Plasma-Induced Nerve Regeneration in a Neuropathic Orofacial Pain Model Saka Winias, Andari Sarasati, Satutya Wicaksono, Nurina Febriyanti Ayuningtyas, Diah Savitri Ernawati, Desiana Radithia European Journal of Dentistry, 2024 Objective Various growth factors contained in PRP can increase angiogenesis and cell proliferation, which plays an essential role in the process of neuroregeneration and peripheral nerve injury recovery. This study analyzed PRP effects in the neuro-regeneration of axonotmesis through brain-derived neurotrophic factor (BDNF) and Krox20 expressions. Materials and Methods Freeze-dried allogeneic platelet-rich plasma (PRP) were prepared from allogeneic sources. Forty-two Rattus norvegicus were divided into three groups: negative control group, positive control group (crushing infraorbital nerve) and treatment group (crushing infraorbital nerve without PRP injection). Each group was observed for fourteen and twenty-one days after injury. Infraorbital nerve tissue is isolated for indirect immunohistochemistry examination with BDNF and Krox20 antibodies. Data analysis was performed using One-Way ANOVA and Mann-Whitney tests with significant value as p < 0.05. Results The PRP group showed BDNF expression significantly higher than control positive groups, both observation days (p = 0.00). A higher Korx20 expression showed by the PRP group after 21 days than in the control positive groups (p = 0.002). Conclusion PRP can potentially improve neuroregeneration of axonotmesis through increased BDNF and Krox20 expression on the twenty-one days after injury.
Basic Fibroblast Growth Factor Expression after Gingival Mesenchymal Stem Cell’s Metabolite Provision in Lipopolysaccharide induce inflammatory bone resorption in vivo Journal of International Dental and Medical Research, 2022
