Augusto Quaresma Henriques Pedro

@ua.pt

Augusto Quaresma Henriques Pedro


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https://researchid.co/augustopedro
35

Scopus Publications

934

Scholar Citations

18

Scholar h-index

31

Scholar i10-index

Scopus Publications

  • Animal waste biomass processing using ionic liquids
    Tiago J.L. Silva, Cariny Polesca, Augusto Q. Pedro, Mara G. Freire
    Ionic Liquids in Biomass Processing Green Protocols, 2026
  • Ionogels as advanced materials for overcoming challenges in wound healing and drug delivery
    Augusto Q. Pedro, Leonor S. Castro, João A.P. Coutinho, Mara G. Freire
    Nano Materials Science, 2025
    Despite relevant advances, the pharmaceutical industry continues to strive with the limited adaptability, moisture management, and discomfort caused by existing wound dressings. Adding to these challenges are the bioavailability and pharmacokinetics of common (bio)therapeutics, overall leading to unmet clinical demands, safety concerns, and poor patient compliance. Ionogels, a versatile class of materials comprising ionic liquids (ILs) confined in an organic or inorganic solid network, have been proposed to overcome these drawbacks. They have demonstrated the ability to enhance the antimicrobial and mechanical properties of the resulting materials while allowing remarkable improvements in drug solubility and their delivery to targeted sites. Nowadays, safety investigations and clinical trials are still required to fully leverage the potential of ionogels for human applications. However, the recent FDA approval of the New Drug Application MRX-5LBT®, a transdermal drug delivery system, opens promising perspectives toward the clinical translation of ionogels. This review focuses on recent advances achieved in the design of ionogels for pharmaceutical applications, viz. in topical formulations to promote wound healing with antimicrobial activity, and as platforms to improve drug pharmacokinetics (solubility and bioavailability), and their delivery at targeted specific sites with controlled release behaviour.
  • Recombinant laccase biosynthesis for efficient polydopamine coating
    Flávia F. Magalhães, Márcia C. Neves, Augusto Q. Pedro, Mara G. Freire, Valéria C. Santos-Ebinuma, Ana P.M. Tavares
    Biochemical Engineering Journal, 2024
    Laccases are versatile biocatalysts with interest for various industrial applications. This study reports the expression of Trametes versicolor laccase in Komagataella phaffii X33. The cultivation parameters (methanol and CuSO 4 concentration, and temperature) for recombinant laccase production were studied in an orbital shaker. Enhanced laccase production was achieved by adding 1 % (v/v) methanol daily, supplementing 0.1 mM CuSO 4 and incubating at 25 °C. Under these conditions, laccase production was scaled-up in a 4 L stirred tank bioreactor. Subsequently, laccase was concentrated and purified using a combined protocol of ultrafiltration and acetone precipitation, achieving a purification factor of 3.02. The laccase produced exhibited robust stability within a pH range from 4.0 to 8.0 and thermal stability up to 30 °C. Michaelis Menten kinetic revealed Michaelis constant (K M ) and maximum rate of reaction (V max ) values of 44.5 µM and 110.9 µM/min, respectively. Finally, laccase was employed as a biocatalyst to assist the polymerization of dopamine to polydopamine, allowing the one-step coating of cellulose filter paper, as confirmed by diffuse reflectance spectroscopy (UV-Vis DRS) and scanning electron microscopy (SEM). This work represents an advance in the field of laccase production in both orbital shaker and bioreactor, while demonstrating, for the first time, the laccase-assisted polymerization of dopamine for the coating of filter paper with polydopamine. Laccase production by Komagataella phaffii and its application in polydopamine synthesis for paper coating • Trametes versicolor laccase was efficiently expressed in Komagataella phaffii . • Laccase production was performed in orbital shaker and bioreactor. • Laccase presented robust stability in the pH range 4.0–8.0 and at room temperature. • A purification factor of 3.02 via ultrafiltration and acetone precipitation steps. • Laccase efficiently assisted the synthesis of polydopamine and paper coating.
  • Towards the improved monitoring of bacterial infections by the isolation of DNA from human serum using ionic-liquid-based aqueous biphasic systems
    Ana M. Ferreira, Maria V. Quental, Mukesh Sharma, Dibyendu Mondal, Augusto Q. Pedro, João A.P. Coutinho, Mara G. Freire
    Separation and Purification Technology, 2023
    Early infection diagnosis is crucial to decrease morbidity and mortality rates. However, complex biological samples, like human blood or serum, contain high abundance proteins and metabolites that reduce the sensitivity of methods used to identify and quantify nucleic acids in bacterial infections diagnosis. To address this issue, we investigated aqueous biphasic systems (ABS) composed of polypropylene glycol 400 and cholinium-based ionic liquids (ILs) at different pH values for the pre-treatment of human serum, aiming the separation of DNA from human serum albumin (HSA) to reduce the interference on the DNA quantification by real-time PCR (qPCR). Remarkable extraction efficiencies of DNA to the IL-rich phase were obtained with all investigated systems, ranging between 90 and 100% in a single-step, with no significant losses of DNA observed (yield at the IL-rich phase > 90%). At low pH values HSA precipitates, whereas at neutral pH no HSA precipitation is observed. This trend suggests that IL-based ABS can be tuned to selectively isolate DNA from HSA by adjusting the pH. The most effective ABS identified is composed of cholinium glycolate at pH 5, allowing to completely precipitate HSA at the ABS interface and leading to an IL-rich phase enriched in DNA with high purity (>98%) that can be quantified by qPCR. Finally, it is shown that the IL-rich phase is able to maintain the DNA’s structural integrity at room temperature, for up to six months, implying that the IL-rich phase of the selected ABS could also be a suitable DNA storage medium. In summary, designed IL-based ABS can be applied as a pretreatment strategy of human serum, allowing to isolate bacterial DNA and opening new perspectives in the monitoring of bacterial infections.
  • Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation
    Catarina Almeida, Augusto Q. Pedro, Ana P. M. Tavares, Márcia C. Neves, Mara G. Freire
    Frontiers in Bioengineering and Biotechnology, 2023
    The emergence of biopharmaceuticals, including proteins, nucleic acids, peptides, and vaccines, revolutionized the medical field, contributing to significant advances in the prophylaxis and treatment of chronic and life-threatening diseases. However, biopharmaceuticals manufacturing involves a set of complex upstream and downstream processes, which considerably impact their cost. In particular, despite the efforts made in the last decades to improve the existing technologies, downstream processing still accounts for more than 80% of the total biopharmaceutical production cost. On the other hand, the formulation of biological products must ensure they maintain their therapeutic performance and long-term stability, while preserving their physical and chemical structure. Ionic-liquid (IL)-based approaches arose as a promise alternative, showing the potential to be used in downstream processing to provide increased purity and recovery yield, as well as excipients for the development of stable biopharmaceutical formulations. This manuscript reviews the most important progress achieved in both fields. The work developed is critically discussed and complemented with a SWOT analysis.
  • Supported ionic liquids as customizable materials to purify immunoglobulin G
    Emanuel V. Capela, Jéssica Bairos, Augusto Q. Pedro, Márcia C. Neves, M. Raquel Aires-Barros, Ana M. Azevedo, João A.P. Coutinho, Ana P.M. Tavares, Mara G. Freire
    Separation and Purification Technology, 2023
    Over the past few years, antibodies such as immunoglobulin G, IgG, have increased their market share as alternative therapeutics. However, their production at high purity levels is still costly due to the absence of a cost-effective platform for their recovery and purification from the complex biological media in which they are produced. This work describes, for the first time, that materials modified with ionic liquids (ILs) can be designed for the effective capture and purification of antibodies from complex matrices, allowing both the selective adsorption of IgG or the selective adsorption of other proteins present in the media. The best results correspond to IgG with 59 % of yield and 84 % of purity in the aqueous solution, and IgG with 76 % of yield and 100 % of purity on the surface of one SIL due to the selective adsorption of IgG from human serum. The best conditions and materials were then applied to other IgG-containing matrices, namely rabbit serum and Chinese hamster ovary (CHO) cell culture supernatants, proving the robustness of the developed strategy. Furthermore, it is demonstrated that the secondary structure of IgG is preserved during the purification process and that these antibodies remain biologically active. In summary, it is shown that by only changing the IL chemical structure at the material surface it is possible to selectively adsorb IgG or to adsorb other proteins leaving IgG in solution. These findings prove that SILs are customizable materials with future potential to act in the flow-through or bind-and-elute modes. Therefore, SILs can be envisioned as potential chromatographic columns capable of substituting the high-cost commercial chromatographic columns based on biological ligands currently used to purify IgG.
  • Corrigendum: Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation (Frontiers in Bioengineering and Biotechnology, (2023), 11, (1037436), 10.3389/fbioe.2023.1037436)
    Catarina Almeida, Augusto Q. Pedro, Ana P. M. Tavares, Márcia C. Neves, Mara G. Freire
    Frontiers in Bioengineering and Biotechnology, 2023
    [This corrects the article DOI: 10.3389/fbioe.2023.1037436.].
  • Thermofluor-Based Optimization Strategy for the Stabilization of Recombinant Human Soluble Catechol-O-Methyltransferase
    Ana M. Gonçalves, Augusto Q. Pedro, Diana M. Oliveira, Adriana E. Oliveira, Marino F. A. Santos, Márcia A. S. Correia, João A. Queiroz, Eugénia Gallardo, Maria J. Romão, Luís A. Passarinha
    International Journal of Molecular Sciences, 2022
    Catechol-O-methyltransferase (COMT) has been involved in a number of medical conditions including catechol-estrogen-induced cancers and a great range of cardiovascular and neurodegenerative diseases such as Parkinson’s disease. Currently, Parkinson’s disease treatment relies on a triple prophylaxis, involving dopamine replacement by levodopa, the use of aromatic L-amino acid decarboxylase inhibitors, and the use of COMT inhibitors. Typically, COMT is highly thermolabile, and its soluble isoform (SCOMT) loses biological activity within a short time span preventing further structural and functional trials. Herein, we characterized the thermal stability profile of lysate cells from Komagataella pastoris containing human recombinant SCOMT (hSCOMT) and enzyme-purified fractions (by Immobilized Metal Affinity Chromatography—IMAC) upon interaction with several buffers and additives by Thermal Shift Assay (TSA) and a biological activity assessment. Based on the obtained results, potential conditions able to increase the thermal stability of hSCOMT have been found through the analysis of melting temperature (Tm) variations. Moreover, the use of the ionic liquid 1-butyl-3-methylimidazolium chloride [C4mim]Cl (along with cysteine, trehalose, and glycerol) ensures complete protein solubilization as well as an increment in the protein Tm of approximately 10 °C. Thus, the developed formulation enhances hSCOMT stability with an increment in the percentage of activity recovery of 200% and 70% when the protein was stored at 4 °C and −80 °C, respectively, for 12 h. The formation of metanephrine over time confirmed that the enzyme showed twice the productivity in the presence of the additive. These outstanding achievements might pave the way for the development of future hSCOMT structural and biophysical studies, which are fundamental for the design of novel therapeutic molecules.
  • Advances in Membrane-Bound Catechol-O-Methyltransferase Stability Achieved Using a New Ionic Liquid-Based Storage Formulation
    Ana M. Gonçalves, Ângela Sousa, Augusto Q. Pedro, Maria J. Romão, João A. Queiroz, Eugénia Gallardo, Luís A. Passarinha
    International Journal of Molecular Sciences, 2022
    Membrane-bound catechol-O-methyltransferase (MBCOMT), present in the brain and involved in the main pathway of the catechol neurotransmitter deactivation, is linked to several types of human dementia, which are relevant pharmacological targets for new potent and nontoxic inhibitors that have been developed, particularly for Parkinson’s disease treatment. However, the inexistence of an MBCOMT 3D-structure presents a blockage in new drugs’ design and clinical studies due to its instability. The enzyme has a clear tendency to lose its biological activity in a short period of time. To avoid the enzyme sequestering into a non-native state during the downstream processing, a multi-component buffer plays a major role, with the addition of additives such as cysteine, glycerol, and trehalose showing promising results towards minimizing hMBCOMT damage and enhancing its stability. In addition, ionic liquids, due to their virtually unlimited choices for cation/anion paring, are potential protein stabilizers for the process and storage buffers. Screening experiments were designed to evaluate the effect of distinct cation/anion ILs interaction in hMBCOMT enzymatic activity. The ionic liquids: choline glutamate [Ch][Glu], choline dihydrogen phosphate ([Ch][DHP]), choline chloride ([Ch]Cl), 1- dodecyl-3-methylimidazolium chloride ([C12mim]Cl), and 1-butyl-3-methylimidazolium chloride ([C4mim]Cl) were supplemented to hMBCOMT lysates in a concentration from 5 to 500 mM. A major potential stabilizing effect was obtained using [Ch][DHP] (10 and 50 mM). From the DoE 146% of hMBCOMT activity recovery was obtained with [Ch][DHP] optimal conditions (7.5 mM) at −80 °C during 32.4 h. These results are of crucial importance for further drug development once the enzyme can be stabilized for longer periods of time.
  • Ionogels for Biomedical Applications
    Bojan Kopilovic, Francisca A. e Silva, Augusto Q. Pedro, João A. P. Coutinho, Mara G. Freire
    Materials Horizons from Nature to Nanomaterials, 2022
  • Efficient isolation of bacterial rnas using silica-based materials modified with ionic liquids
    Patrícia Pereira, Augusto Q. Pedro, Márcia C. Neves, João C. Martins, Inês Rodrigues, Mara G. Freire, Fani Sousa
    Life, 2021
  • Impact of glycerol feeding profiles on STEAP1 biosynthesis by Komagataella pastoris using a methanol-inducible promoter
    D. R. Duarte, J. Barroca-Ferreira, A. M. Gonçalves, F. M. Santos, S. M. Rocha, A. Q. Pedro, C. J. Maia, L. A. Passarinha
    Applied Microbiology and Biotechnology, 2021
  • Interferon-based biopharmaceuticals: Overview on the production, purification, and formulation
    Leonor S. Castro, Guilherme S. Lobo, Patrícia Pereira, Mara G. Freire, Márcia C. Neves, Augusto Q. Pedro
    Vaccines, 2021
  • Nucleolin as a potential biomarker for canine malignant neoplasia
    Carla Cruz, Augusto Q. Pedro, Josué Carvalho, Tiago Santos, Daniela Talhada, Artur Paiva, João A. Queiroz, Mónica Andrade, Marta Pinto, Luís Montenegro, Leonor Delgado, Patrícia Pereira
    Research in Veterinary Science, 2021
  • Enhanced performance of polymer-polymer aqueous two-phase systems using ionic liquids as adjuvants towards the purification of recombinant proteins
    Leonor S. Castro, Patrícia Pereira, Luís A. Passarinha, Mara G. Freire, Augusto Q. Pedro
    Separation and Purification Technology, 2020
  • Brain-targeted delivery of pre-mir-29b using lactoferrin-stearic acid-modified-chitosan/ polyethyleneimine polyplexes
    Patrícia Pereira, Maria Barreira, Carla Cruz, Joana Tomás, Ângelo Luís, Augusto Q. Pedro, João A. Queiroz, Fani Sousa
    Pharmaceuticals, 2020
  • Improved ionic-liquid-functionalized macroporous supports able to purify nucleic acids in one step
    M.C. Neves, P. Pereira, A.Q. Pedro, J.C. Martins, T. Trindade, J.A. Queiroz, M.G. Freire, F. Sousa
    Materials Today Bio, 2020
  • Effect of chromatographic conditions on supercoiled plasmid DNA stability and bioactivity
    G.M. Azevedo, J.F.A. Valente, A. Sousa, A.Q. Pedro, P. Pereira, F. Sousa, J.A. Queiroz
    Applied Sciences Switzerland, 2019
  • Smoothing membrane protein structure determination by initial upstream stage improvements
    Augusto Quaresma Pedro, João António Queiroz, Luís António Passarinha
    Applied Microbiology and Biotechnology, 2019
  • Integrated Extraction-Preservation Strategies for RNA Using Biobased Ionic Liquids
    Maria V. Quental, Augusto Q. Pedro, Patrícia Pereira, Mukesh Sharma, João A. Queiroz, João A. P. Coutinho, Fani Sousa, Mara G. Freire
    ACS Sustainable Chemistry and Engineering, 2019
  • Cholinium-Based Good's Buffers Ionic Liquids as Remarkable Stabilizers and Recyclable Preservation Media for Recombinant Small RNAs
    Augusto Q. Pedro, Patrícia Pereira, Maria J. Quental, André P. Carvalho, Sérgio M. Santos, João A. Queiroz, Fani Sousa, Mara G. Freire
    ACS Sustainable Chemistry and Engineering, 2018
  • Purification of Histidine-Tagged Membrane-Bound Catechol-O-Methyltransferase from Detergent-Solubilized Pichia pastoris Membranes
    A. Q. Pedro, A. M. Gonçalves, J. A. Queiroz, L. A. Passarinha
    Chromatographia, 2018
  • New insights for therapeutic recombinant human miRNAs heterologous production: Rhodovolum sulfidophilum vs Escherichia coli
    Patrícia Pereira, Augusto Q. Pedro, João A. Queiroz, Ana R. Figueiras, Fani Sousa
    Bioengineered, 2017
  • Biosynthesis and purification of histidine-tagged human soluble catechol-O-methyltransferase
    Augusto Q Pedro, Filipa F Correia, Fátima M Santos, Guilherme Espírito-Santo, Ana M Gonçalves, Maria J Bonifácio, João A Queiroz, Luís A Passarinha
    Journal of Chemical Technology and Biotechnology, 2016
  • Advances in time course extracellular production of human pre-miR-29b from Rhodovulum sulfidophilum
    Patrícia Pereira, Augusto Q. Pedro, Joana Tomás, Cláudio J. Maia, João A. Queiroz, Ana Figueiras, Fani Sousa
    Applied Microbiology and Biotechnology, 2016
  • Purification of Membrane-Bound Catechol-O-Methyltransferase by Arginine-Affinity Chromatography
    A. Q. Pedro, P. Pereira, M. J. Bonifácio, J. A. Queiroz, L. A. Passarinha
    Chromatographia, 2015
  • An artificial neural network for membrane-bound catechol-O-methyltransferase biosynthesis with Pichia pastoris methanol-induced cultures
    Augusto Q Pedro, Luís M Martins, João M L Dias, Maria J Bonifácio, João A Queiroz, Luís A Passarinha
    Microbial Cell Factories, 2015
  • Enhanced biosynthesis of plasmid DNA from Escherichia coli VH33 using Box-Behnken design associated to aromatic amino acids pathway
    L.M. Martins, A.Q. Pedro, D. Oppolzer, F. Sousa, J.A. Queiroz, L.A. Passarinha
    Biochemical Engineering Journal, 2015
  • Evaluation of MutS and Mut+ Pichia pastoris Strains for Membrane-Bound Catechol-O-Methyltransferase Biosynthesis
    A. Q. Pedro, D. Oppolzer, M. J. Bonifácio, C. J. Maia, J. A. Queiroz, L. A. Passarinha
    Applied Biochemistry and Biotechnology, 2015
  • Recovery of biological active catechol-O-methyltransferase isoforms from Q-sepharose
    F. F. Correia, F. M. Santos, A. Q. Pedro, M. J. Bonifácio, J. A. Queiroz, L. A. Passarinha
    Journal of Separation Science, 2014
  • Development of fed-batch profiles for efficient biosynthesis of catechol-O-methyltransferase
    G.M. Espírito Santo, A.Q. Pedro, D. Oppolzer, M.J. Bonifácio, J.A. Queiroz, F. Silva, L.A. Passarinha
    Biotechnology Reports, 2014
  • Trends in protein-based biosensor assemblies for drug screening and pharmaceutical kinetic studies
    Ana Gonçalves, Augusto Pedro, Fátima Santos, Luís Martins, Cláudio Maia, João Queiroz, Luís Passarinha
    Molecules, 2014
  • Pichia pastoris: A recombinant microfactory for antibodies and human membrane proteins
    A. M. Goncalves
    Journal of Microbiology and Biotechnology, 2013
  • Performance of hydrophobic interaction ligands for human membrane-bound catechol-O-methyltransferase purification
    Fátima Milhano Santos, Augusto Quaresma Pedro, Rui Filipe Soares, Rita Martins, Maria João Bonifácio, João António Queiroz, Luís António Passarinha
    Journal of Separation Science, 2013
  • A novel prokaryotic expression system for biosynthesis of recombinant human membrane-bound catechol-O-methyltransferase
    A.Q. Pedro, M.J. Bonifácio, J.A. Queiroz, C.J. Maia, L.A. Passarinha
    Journal of Biotechnology, 2011

RECENT SCHOLAR PUBLICATIONS

  • Animal waste biomass processing using ionic liquids
    TJL Silva, C Polesca, AQ Pedro, MG Freire
    Ionic Liquids in Biomass Processing, 205-252 , 2026
    2026
  • Extraction and clarification of mrna from yeast using aqueous biphasic systems
    MF Galrinho, JL Fernandes, ASC Marques, APM Tavares, AQ Pedro, ...
    Research summit 2025-Book of abstracts, 204 , 2025
    2025
  • Deep eutectic solvents as a novel class of excipients for messenger rna-based formulations toward enhanced stability
    ASC Marques, AQ Pedro, APM Tavares, MG Freire
    Research summit 2025-Book of abstracts, 207 , 2025
    2025
  • Amino acid-based ils as new excipients for small rnas stabilization
    AF Pereira, AQ Pedro, APM Tavares, LC Branco, J AP, FS Coutinho, ...
    Research summit 2025-Book of abstracts, 186 , 2025
    2025
  • Ionic liquid mediated recovery of recombinant nucleic-acid biopharmaceuticals.
    ANAPMT GONÇALVES, C PICHON, AQ PEDRO, MG FREIRE
    Research summit 2025-Book of abstracts, 196 , 2025
    2025
  • Bioproduction of messenger RNA therapeutics in yeast cells: a proof of concept study
    V Bilger, K Pachulska-Wieczorek, F Daboussi, M Freire, U Cernigoj, ...
    MOLECULAR THERAPY 33 (4) , 2025
    2025
  • Recombinant laccase biosynthesis for efficient polydopamine coating
    FF Magalhães, MC Neves, AQ Pedro, MG Freire, VC Santos-Ebinuma, ...
    Biochemical Engineering Journal 212, 109483 , 2024
    2024
    Citations: 10
  • Integrated process for the recovery and purification of recombinant messenger RNA vaccines resorting to ionic liquids
    ASC Marques, AQ Pedro, APM Tavares, MG Freire
    Research summit 2024-Book of abstracts, 193 , 2024
    2024
  • Three-phase partitioning systems based on biocompatible ionic liquids towards the efficient purification of bacterial RNAs from cell lysates
    AF Pereira, AQ Pedro, LS Castro, MJ Quental, A PM, LC Tavares, ...
    Research summit 2024-Book of abstracts, 140 , 2024
    2024
  • Customized ionic liquid patches for efficient and non-invasive transdermal delivery of messenger RNA vaccines
    JL Fernandes, AQ Pedro, MG Freire
    Research summit 2024-Book of abstracts, 186 , 2024
    2024
  • Ionic-liquid-based aqueous biphasic systems for minicircle DNA recovery from cell lysates
    AI Valente, JV Valente, A De Francesco, AQ Pedro, A PM, FS Tavares, ...
    Research summit 2024-Book of abstracts, 205 , 2024
    2024
  • Unlocking the potential of polymeric ionic liquids as chromatographic supports for recombinant protein downstream processing
    LS Castro, AQ Pedro, MC Neves, IM Marrucho, MG Freire
    Research summit 2024-Book of abstracts, 187 , 2024
    2024
  • Ionogels as advanced materials for overcoming challenges in wound healing and drug delivery
    AQ Pedro, LS Castro, JAP Coutinho, MG Freire
    Nano Materials Science , 2024
    2024
    Citations: 17
  • lonic-liquid-based approaches to improve biopharmaceuticals downstream processing and
    C Almeida, AQ Pedro, APM Tavares
    Celebrating 20 Years of CICECO–Aveiro Institute of Materials-Current and … , 2023
    2023
  • Towards the improved monitoring of bacterial infections by the isolation of DNA from human serum using ionic-liquid-based aqueous biphasic systems
    AM Ferreira, MV Quental, M Sharma, D Mondal, AQ Pedro, JAP Coutinho, ...
    Separation and Purification Technology 316, 123801 , 2023
    2023
    Citations: 9
  • Corrigendum: Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation
    C Almeida, AQ Pedro, APM Tavares, MC Neves, MG Freire
    Frontiers in Bioengineering and Biotechnology 11, 1213921 , 2023
    2023
    Citations: 2
  • Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation
    C Almeida, AQ Pedro, APM Tavares, MC Neves, MG Freire
    Frontiers in Bioengineering and Biotechnology 11, 1037436 , 2023
    2023
    Citations: 26
  • Immobilization of ionic liquids, types of materials, and applications
    AQ Pedro, JAP Coutinho, MG Freire
    Encyclopedia of ionic liquids, 488-499 , 2023
    2023
    Citations: 21
  • Supported ionic liquids as customizable materials to purify immunoglobulin G
    EV Capela, J Bairos, AQ Pedro, MC Neves, MR Aires-Barros, ...
    Separation and Purification Technology 305, 122464 , 2023
    2023
    Citations: 17
  • Thermofluor-Based Optimization Strategy for the Stabilization of Recombinant Human Soluble Catechol- O -Methyltransferase
    AM Gonçalves, AQ Pedro, DM Oliveira, AE Oliveira, MFA Santos, ...
    International Journal of Molecular Sciences 23 (20), 12298 , 2022
    2022
    Citations: 6

MOST CITED SCHOLAR PUBLICATIONS

  • Pichia pastoris: a recombinant microfactory for antibodies and human membrane proteins
    AM Goncalves, AQ Pedro, C Maia, F Sousa, JA Queiroz, LA Passarinha
    Korean Society for Microbiology and Biotechnology 23 (5), 587-601 , 2013
    2013
    Citations: 108
  • Interferon-based biopharmaceuticals: Overview on the production, purification, and formulation
    LS Castro, GS Lobo, P Pereira, MG Freire, MC Neves, AQ Pedro
    Vaccines 9 (4), 328 , 2021
    2021
    Citations: 63
  • Enhanced performance of polymer-polymer aqueous two-phase systems using ionic liquids as adjuvants towards the purification of recombinant proteins
    LS Castro, P Pereira, LA Passarinha, MG Freire, AQ Pedro
    Separation and Purification Technology 248, 117051 , 2020
    2020
    Citations: 60
  • Cholinium-based Good’s buffers ionic liquids as remarkable stabilizers and recyclable preservation media for recombinant small RNAs
    AQ Pedro, P Pereira, MJ Quental, AP Carvalho, SM Santos, JA Queiroz, ...
    ACS sustainable chemistry & engineering 6 (12), 16645-16656 , 2018
    2018
    Citations: 49
  • Evaluation of Mut S and Mut + Pichia pastoris Strains for Membrane-Bound Catechol- O -Methyltransferase Biosynthesis
    AQ Pedro, D Oppolzer, MJ Bonifácio, CJ Maia, JA Queiroz, LA Passarinha
    Applied biochemistry and biotechnology 175 (8), 3840-3855 , 2015
    2015
    Citations: 45
  • Trends in protein-based biosensor assemblies for drug screening and pharmaceutical kinetic studies
    AM Gonçalves, AQ Pedro, FM Santos, LM Martins, CJ Maia, JA Queiroz, ...
    Molecules 19 (8), 12461-12485 , 2014
    2014
    Citations: 41
  • Integrated extraction-preservation strategies for RNA using biobased ionic liquids
    MV Quental, AQ Pedro, P Pereira, M Sharma, JA Queiroz, JAP Coutinho, ...
    ACS Sustainable Chemistry & Engineering 7 (10), 9439-9448 , 2019
    2019
    Citations: 35
  • Advances in time course extracellular production of human pre-miR-29b from Rhodovulum sulfidophilum
    P Pereira, AQ Pedro, J Tomás, CJ Maia, JA Queiroz, A Figueiras, F Sousa
    Applied microbiology and biotechnology 100 (8), 3723-3734 , 2016
    2016
    Citations: 34
  • New insights for therapeutic recombinant human miRNAs heterologous production: Rhodovolum sulfidophilum vs Escherichia coli
    P Pereira, AQ Pedro, JA Queiroz, AR Figueiras, F Sousa
    Bioengineered 8 (5), 670-677 , 2017
    2017
    Citations: 32
  • Brain-targeted delivery of pre-miR-29b using lactoferrin-stearic acid-modified-chitosan/polyethyleneimine polyplexes
    P Pereira, M Barreira, C Cruz, J Tomás, Â Luís, AQ Pedro, JA Queiroz, ...
    Pharmaceuticals 13 (10), 314 , 2020
    2020
    Citations: 29
  • A novel prokaryotic expression system for biosynthesis of recombinant human membrane-bound catechol-O-methyltransferase
    AQ Pedro, MJ Bonifácio, JA Queiroz, CJ Maia, LA Passarinha
    Journal of biotechnology 156 (2), 141-146 , 2011
    2011
    Citations: 29
  • Ionic-liquid-based approaches to improve biopharmaceuticals downstream processing and formulation
    C Almeida, AQ Pedro, APM Tavares, MC Neves, MG Freire
    Frontiers in Bioengineering and Biotechnology 11, 1037436 , 2023
    2023
    Citations: 26
  • An artificial neural network for membrane-bound catechol- O -methyltransferase biosynthesis with Pichia pastoris methanol-induced cultures
    AQ Pedro, LM Martins, JML Dias, MJ Bonifácio, JA Queiroz, ...
    Microbial cell factories 14 (1), 113 , 2015
    2015
    Citations: 25
  • Improved ionic-liquid-functionalized macroporous supports able to purify nucleic acids in one step
    MC Neves, P Pereira, AQ Pedro, JC Martins, T Trindade, JA Queiroz, ...
    Materials Today Bio 8, 100086 , 2020
    2020
    Citations: 24
  • Recovery of biological active catechol‐ O ‐methyltransferase isoforms from Q‐sepharose
    FF Correia, FM Santos, AQ Pedro, MJ Bonifácio, JA Queiroz, ...
    Journal of separation science 37 (1-2), 20-29 , 2014
    2014
    Citations: 24
  • Immobilization of ionic liquids, types of materials, and applications
    AQ Pedro, JAP Coutinho, MG Freire
    Encyclopedia of ionic liquids, 488-499 , 2023
    2023
    Citations: 21
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