Current research involves the proteomics and metabolomics studies of diseases such as the ultra-rare metabolic disorder Alkaptonuria, the rare malignancies soft tissue sarcomas, steroid converting enzymes linked diseases such as lipedema and lymphedema, by use of NMR, MS, and computational methods.
104
Scopus Publications
Scopus Publications
Exclusion of CLIC5 as a Candidate Gene and Identification of NEFM as a Possible Novel Gene Correlated With Autosomal Recessive Pure Cerebellar Ataxia in a Highly Consanguineous Family Paolo Enrico Maltese, Gabriele Bonetti, Elena Manara, Benedetta Tanzi, Andrea Bernini, Mark A. Berryman, Soichi Tanda, Corinne Nielsen, Silvia Casagrande, Amanda Ferrero, Salvatore Stano, Riccardo Zuccarino, Andrea Barp, Pietro Chiurazzi, Matteo Bertelli Molecular Genetics and Genomic Medicine, 2026 Background Pure cerebellar ataxia is a neurological disorder characterised by isolated cerebellar dysfunction, arising from either developmental anomalies or progressive degenerative processes. Precise genetic diagnosis remains challenging. Methods The aim of this study was to use a whole‐exome sequencing approach to study a large, highly consanguineous Italian family in order to identify a new gene correlated with pure cerebellar ataxia. Results Sequencing excluded the presence of mutations in known‐related genes but revealed a homozygous missense variant in CLIC5 ; however in vivo analysis of a CLIC5 KO mouse model showed vestibular dysfunction without cerebellar involvement, suggesting that CLIC5 is not directly involved in pure cerebellar ataxia onset. Further analysis identified two compound heterozygous variants in NEFM , and in silico analysis showed that they dysregulate NEFM phosphorylation. Phosphorylation of neurofilaments and subsequent formation of aggregates has already been linked to conditions such as ageffing and neurodegeneration. Moreover, in vivo studies on mice transgenic for human NEFM have correlated NEFM phosphorylation and aggregation with neurodegeneration. Finally, neurofilaments have been proposed to be correlated to ataxia and autoimmune cerebellar ataxia. Conclusion We therefore propose NEFM as a possible new candidate gene for hereditary cerebellar ataxia. These findings could be useful for advancing the genetic diagnosis of hereditary pure cerebellar ataxia, possibly enabling the screening of healthy carriers.
Metabolomics Plasma Biomarkers Associated with the HRD Phenotype in Ovarian Cancer Alessandro Tubita, Claudia De Angelis, Daniela Grasso, Flavia Sorbi, Francesca Castiglione, Lorenzo Anela, Maria Cristina Petrella, Massimiliano Fambrini, Federico Scolari, Andrea Bernini, Giulia Petroni, Serena Pillozzi, Lorenzo Antonuzzo Metabolites, 2026 Background: Ovarian cancer (OC) remains one of the most lethal gynecologic malignancies due to its often-late diagnosis and complex molecular heterogeneity. Understanding the metabolic alterations in OC can provide insights into its pathophysiology and potential therapeutic targets. This study aimed to explore serum metabolomic profiles and their correlation with clinical and pathological features in OC patients. Materials and Methods: Thirty serum samples were collected from patients diagnosed with ovarian tumors (OTs) (n = 24 malignant, n = 6 benign) and undergoing treatment at Careggi University Hospital. Additionally, 47 samples were obtained from age-matched healthy female donors. Serum samples underwent processing and analysis using an H-NMR (Nuclear Magnetic Resonance) platform to identify a panel of metabolites. Correlation analysis between the metabolomic data and clinical parameters was performed using R software (v.4.4.0). Results: Differential metabolomic profiling showed a significant upregulation of metabolites associated with the purine salvage pathway (i.e., hypoxanthine and inosine) and the ketone bodies axis (i.e., acetone, 3-hydroxybutyrate, and acetate) in samples from ovarian tumor (OT) patients compared to healthy donors. Within malignant OC samples, metabolomic profiles significantly correlated with BRCA1/2 mutation status (BRCA1/2-mutated vs. wild-type) and homologous recombination deficiency (HRD) status. Conclusions: The analysis revealed significant variation in specific metabolites such as betaine, creatinine, carnitine, glycerol, and mannose; notably, a downregulation of these metabolites was observed in HRD-positive patients. The study identifies significant metabolomic alterations in OC, implicating pathways such as purine salvage and ketone bodies. Intriguingly, consistent variation in specific metabolites across BRCA/HRD phenotypes underscores their potential as OC biomarkers. Further research is needed to validate these findings and explore their prognostic and therapeutic implications.
Serum Biomarkers in Bladder Cancer: NMR Metabolomics for Identification and Monitoring during Platinum-Based Therapy Roberta Giorgione, Daniela Grasso, Elisabetta Gambale, Federico Scolari, Virginia Rossi, Fabrizio Di Maida, Marinella Micol Mela, Barbara Marzocchi, Laura Doni, Adriano Pasqui, Andrea Minervini, Enrico Caliman, Sergio Serni, Andrea Bernini, Serena Pillozzi, Lorenzo Antonuzzo Oncology Research, 2026 Objectives: To date, predictive and prognostic biomarkers for Bladder Cancer (BC) remain lacking. Existing literature underscores the potential of metabolomics as a valuable tool for biomarker identification. The primary objective of this study is to characterize the serum metabolic profile of BC patients undergoing platinum-based chemotherapy (Pt-CT) to identify potential biomarkers. Methods: In this pilot study, we investigated the metabolomic profiles of 14 BC patients undergoing Pt-CT in different settings. We compared their baseline profiles with those of healthy controls and tracked key metabolites throughout chemotherapy cycles. Metabolomics profiling was conducted using nuclear magnetic resonance (NMR) spectroscopy. All experiments were performed on a Bruker Avance™ 600 spectrometer. Results: Serum samples of BC patients had elevated levels of acetate, acetone, hypoxanthine, trimethylamine N-oxide (TMAO), glutamate, lactate, phenylalanine, and ornithine. Conversely, there were decreased levels of carnitine, choline, betaine, aspartate, threonine, 2-hydroxybutyrate, 2-aminobutyrate and histidine when compared with healthy controls. Throughout the CT course, hypoxanthine, glutamate, and aspartate levels increased, while acetone, acetate and TMAO levels decreased. Conclusions: The results of our study confirm perturbations in several metabolic pathways in the serum samples of BC patients, including glycolysis, fatty acid, purine, and amino acid metabolism. Additionally, TMAO may contribute to BC development by fostering a pro-inflammatory and oxidative stress state. Furthermore, monitoring these metabolites could serve as a valuable tool for predicting treatment response. To the best of our knowledge, no metabolomic studies have assessed BC patients undergoing CT with longitudinal monitoring to identify changes in the metabolic profile induced by treatment.
Th17-like cells and immunosuppressive macrophages infiltrate tertiary lymphoid structures with distinct maturation status in soft-tissue sarcoma Giulia Petroni, Federico Scolari, Guido Scoccianti, Annarita Palomba, Daniela Greto, Simone Romagnoli, Ilaria Palchetti, Andrea Bernini, Enrico Caliman, Simone Polvani, Filippo Nozzoli, Beatrice Menicacci, Giulia Nannini, Domenico Andrea Campanacci, Serena Pillozzi, Lorenzo Antonuzzo Cell Death and Disease, 2025 Tertiary lymphoid structures (TLSs) have been associated with favorable clinical outcome and improved responses to immune checkpoint inhibitors in soft-tissue sarcomas (STSs). However, due to their rarity and high heterogeneity, information regarding the mechanisms involved in TLS formation and contribution to antitumor immunity in STS are extremely elusive. To address this gap, we integrated immunohistochemistry and transcriptomic analyses from 31 treatment-naïve STS specimens from an independent cohort of patients with primary or locally recurrent disease. Further validation was conducted using external bulk, single-cell and spatial transcriptomics data from 5 publicly available datasets. We found TLSs to be highly heterogeneous in terms of amount, localization, maturation status and cellular composition, and corroborated previous findings showing that their presence, along with B cells in the STS microenvironment, are good indicators of favorable prognosis. Transcriptomic analysis showed that high expression of germinal center (GC) B cell-related genes was associated with TLS presence and with an upregulation of signatures specific for T helper 17 (Th17) cells in STS and other cancer types. Conversely, genes signatures discriminating for immunosuppressive M2-like macrophages were enriched in tumors with low expression of GC B cell-related genes. Immunohistochemistry showed distinct spatial patterns for Th17-like cells and M2-like macrophages within TLS areas, with IL17A + cells predominantly localized within intratumoral mature TLSs, and CD163 + macrophages mainly observed in immature TLSs. Integrating these findings, we identified tumors with high expression of GC B cell- and Th17 cell-related signatures together with low fractions of M2-like macrophages, to have superior survival outcomes. Herein, our findings point out to two cellular players (Th17-like cells and M2-like macrophages) with potentially opposite roles in STS-associated TLS formation and maturation, thus providing the basis for future research efforts aiming at the development of therapeutic immunological interventions to enhance TLS-mediated antitumor immunity in STS.
Ochronotic Deposition in Alkaptonuria: Semiquinone-Mediated Oxidative Coupling and Metabolic Drivers of Homogentisic Acid Accumulation Daniela Grasso, Valentina Balloni, Maria Camilla Baratto, Adele Mucci, Annalisa Santucci, Andrea Bernini International Journal of Molecular Sciences, 2025 Alkaptonuria (AKU) is a rare metabolic disorder caused by homogentisate 1,2-dioxygenase (HGD) deficiency, leading to homogentisic acid (HGA) accumulation and ochronotic pigment deposition, which drug therapy cannot reverse. The process of pigment formation and deposition is still unclear. This study offers molecular insights into the polymeric structure, with the goal of developing future adjuvant strategies that can inhibit or reverse pigment formation, thereby complementing drug therapy in AKU. HGA polymerisation was examined under physiological, acidic, and alkaline conditions using liquid and solid phase nuclear magnetic resonance (NMR), electron paramagnetic resonance (EPR), and polyacrylamide gel electrophoresis. At physiological pH, HGA polymerised slowly, while alkaline catalysis accelerated pigment formation while retaining the HGA aromatic scaffold. During the process, EPR detected a semiquinone radical intermediate, consistent with an oxidative coupling mechanism. Reactivity profiling showed the diphenol ring was essential for polymerisation, while –CH2COOH modifications did not impair reactivity. Pigments displayed a polydisperse molecular weight range (11–50 kDa) and a strong negative charge. Solid-state NMR has revealed the presence of phenolic ether and biphenyl linkages. Collectively, these identified structural motifs can serve as a foundation for future molecular targeting related to pigment formation.
Untargeted Metabolomics and Liquid Biopsy Investigation of Circulating Biomarkers in Soft Tissue Sarcoma Daniela Grasso, Barbara Marzocchi, Guido Scoccianti, Ilaria Palchetti, Domenico Andrea Campanacci, Lorenzo Antonuzzo, Federico Scolari, Serena Pillozzi, Andrea Bernini Cancers, 2025 Background: Soft tissue sarcomas (STSs) are rare, highly malignant mesenchymal tumours, comprising approximately 1% of all adult cancers and about 15% of paediatric solid tumours. STSs exhibit considerable genomic complexity with diverse subtypes, posing significant clinical challenges. Objectives: This study aims to characterise the molecular signature of primary STS through liquid biopsies and the untargeted metabolomic profiling of 75 patients, providing deep insights into cellular processes and potential therapeutic targets. Methods: This study analysed serum samples using nuclear magnetic resonance (NMR) spectroscopy for metabolomic profiling. Multivariate data analysis and machine learning classifiers were employed to identify biomarkers. Results: A panel of eleven significant deregulated metabolites were discovered in serum samples of patients with STS, with potential implications for cancer diagnosis and treatment. Conclusions: Choline decrease emerged as a marker for cancer progression, highlighting the potential of targeting its metabolism for therapeutic approaches in STS. The NMR analysis protocol proved effective for determining circulating biomarkers from liquid biopsies, making it suitable for rare disease research.
Antioxidant Bio-Compounds from Chestnut Waste: A Value-Adding and Food Sustainability Strategy Roberta Barletta, Alfonso Trezza, Andrea Bernini, Lia Millucci, Michela Geminiani, Annalisa Santucci Foods, 2025 In an era of escalating environmental challenges, converting organic residues into high-value bioactive compounds provides a sustainable way to reduce waste and enhance resource efficiency. This study explores the potential of the circular bioeconomy through the valorization of agricultural byproducts, with a focus on the antioxidant properties of specific chestnut burr cultivars. Currently, over one-third of food production is wasted, contributing to both humanitarian and environmental crises. Through circular bioeconomy, we can transform biological waste into valuable products for use in fields like food innovation and sustainability. The antioxidant effects of three chestnut cultivars, Bastarda Rossa, Cecio, and Marroni, were assessed through in vitro assays, highlighting their potential to combat oxidative stress—an important factor for health-related applications. The characterization of the three cultivars showed the major presence of ellagic acid and gallic acid in the extract, renowned for their antioxidant activity. In vitro assays evaluated the phenolic and flavonoid content, as well as the antioxidant activity of the three extracts, confirming the cultivar Cecio as the richest in these bioactive compounds and the most performative in antioxidant assays. In vitro antioxidant and oxidative stress recovery assays on SaOS-2, fibroblast, and chondrocyte cell lines displayed a strong antioxidant activity. Furthermore, the cytotoxicity assay demonstrated the safety of all three extracts in the tested human cell lines. In silico docking simulations further validated the biological relevance of these compounds by predicting strong hydrophobic and polar interactions with oxidative stress-related protein targets. Overall, this study demonstrates the antioxidant properties of chestnut byproducts. The findings contribute to the development of functional foods, nutraceuticals, and other applications, underscoring the role of chestnut cultivars in advancing circular bioeconomy practices.
Molecular Dynamics Simulations Suggest That Side-Chain Motions of Charged Amino Acids Determine Long-Range Effects in Proteins: An Egg of Coulomb Neri Niccolai, Edoardo Morandi, Andrea Bernini International Journal of Molecular Sciences, 2024 Living systems cannot rely on random intermolecular approaches toward cell crowding, and hidden mechanisms must be present to favor only those molecular interactions required explicitly by the biological function. Electromagnetic messaging among proteins is proposed from the observation that charged amino acids located on the protein surface are mostly in adjacent sequence positions and/or in spatial proximity. Molecular dynamics (MD) simulations have been used to predict electric charge proximities arising from concerted motions of charged amino acid side chains in two protein model systems, human ubiquitin and the chitinolytic enzyme from Ostrinia furnacalis. This choice has been made for their large difference in size and sociality. Protein electrodynamics seems to emerge as the framework for a deeper understanding of the long-distance interactions of proteins with their molecular environment. Our findings will be valuable in orienting the design of proteins with specific recognition patterns.
Antibacterial and Anti-Inflammatory Activity of Branched Peptides Derived from Natural Host Defense Sequences Giada Meogrossi, Eva Tollapi, Alessandro Rencinai, Jlenia Brunetti, Silvia Scali, Eugenio Paccagnini, Mariangela Gentile, Pietro Lupetti, Simona Pollini, Gian Maria Rossolini, Andrea Bernini, Alessandro Pini, Luisa Bracci, Chiara Falciani Journal of Medicinal Chemistry, 2024 Antibiotic resistance is a major global health threat, necessitating the development of new treatments and diverse molecules to combat severe infections and preserve the efficacy of existing drugs. Antimicrobial peptides (AMPs) offer a versatile arsenal against bacteria, and peptide structure branching can enhance their resistance to proteases and improve their overall efficacy. A small library of peptides derived from natural host defense peptides and synthesized in a tetrabranched form was selected against E. coli. Six selected branched peptides were further studied for antibacterial activity against a panel of strains, biofilm inhibition, protease resistance, and cytotoxicity. Their structure was predicted computationally and their mechanism of action was investigated by electron microscopy and by using fluorescent dyes. The peptide BAMP2 showed promise in a mouse skin infection model, indicating the potential for local infection treatment.
Phytochemical Composition, Anti-Inflammatory Property, and Anti-Atopic Effect of Chaetomorpha linum Extract Luisa Frusciante, Michela Geminiani, Alfonso Trezza, Tommaso Olmastroni, Pierfrancesco Mastroeni, Laura Salvini, Stefania Lamponi, Andrea Bernini, Daniela Grasso, Elena Dreassi, Ottavia Spiga, Annalisa Santucci Marine Drugs, 2024 Utilizing plant-based resources, particularly their by-products, aligns with sustainability principles and circular bioeconomy, contributing to environmental preservation. The therapeutic potential of plant extracts is garnering increasing interest, and this study aimed to demonstrate promising outcomes from an extract obtained from an underutilized plant waste. Chaetomorpha linum, an invasive macroalga found in the Orbetello Lagoon, thrives in eutrophic conditions, forming persistent mats covering approximately 400 hectares since 2005. The biomass of C. linum undergoes mechanical harvesting and is treated as waste, requiring significant human efforts and economic resources—A critical concern for municipalities. Despite posing challenges to local ecosystems, the study identified C. linum as a natural source of bioactive metabolites. Phytochemical characterization revealed lipids, amino acids, and other compounds with potential anti-inflammatory activity in C. linum extract. In vitro assays with LPS-stimulated RAW 264.7 and TNF-α/IFN-γ-stimulated HaCaT cells showed the extract inhibited reactive oxygen species (ROS), nitric oxide (NO), and prostaglandin E2 (PGE2) productions, and reduced inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expressions via NF-κB nuclear translocation, in RAW 264.7 cells. It also reduced chemokines (TARC/CCL17, RANTES/CCL5, MCP-1/CCL2, and IL-8) and the cytokine IL-1β production in HaCaT cells, suggesting potential as a therapeutic candidate for chronic diseases like atopic dermatitis. Finally, in silico studies indicated palmitic acid as a significant contributor to the observed effect. This research not only uncovered the untapped potential of C. linum but also laid the foundation for its integration into the circular bioeconomy, promoting sustainable practices, and innovative applications across various industries.
Towards a Long-Read Sequencing Approach for the Molecular Diagnosis of RPGRORF15 Genetic Variants Gabriele Bonetti, William Cozza, Andrea Bernini, Jurgen Kaftalli, Chiara Mareso, Francesca Cristofoli, Maria Chiara Medori, Leonardo Colombo, Salvatore Martella, Giovanni Staurenghi, Anna Paola Salvetti, Benedetto Falsini, Giorgio Placidi, Marcella Attanasio, Grazia Pertile, Mario Bengala, Francesca Bosello, Antonio Petracca, Fabiana D’Esposito, Benedetta Toschi, Paolo Lanzetta, Federico Ricci, Francesco Viola, Giuseppe Marceddu, Matteo Bertelli International Journal of Molecular Sciences, 2023
Omics sciences and precision medicine in breast and ovarian cancer G. Bonetti, G. Madeo, S. Michelini, M. Ricci, M. Cestari, S. Michelini, M. Gadler, S. Benedetti, G. Guerri, F. Cristofoli, D. Generali, C. A. Donofrio, M. Cominetti, A. Fioravanti, L. Riccio, A. Bernini, E. Fulcheri, L. Stuppia, V. Gatta, S. Cecchin, G. Marceddu, M. Bertelli La Clinica Terapeutica, 2023
Omics sciences and precision medicine in lung cancer C. Micheletti, K. Dhuli, K. Donato, M. Gadler, S. Benedetti, G. Guerri, F. Cristofoli, D. Generali, C. A. Donofrio, M. Cominetti, A. Fioravanti, L. Riccio, A. Bernini, E. Fulcheri, L. Stuppia, L. Stuppia, V. Gatta, S. Cristoni, S. Cecchin, G. Marceddu, M. Bertelli La Clinica Terapeutica, 2023
Omics sciences and precision medicine in glioblastoma C. Micheletti, G. Bonetti, G. Madeo, M. Gadler, S. Benedetti, G. Guerri, F. Cristofoli, D. Generali, C. A. Donofrio, M. Cominetti, A. Fioravanti, L. Riccio, P. Manganotti, P. Caruso, A. Bernini, E. Fulcheri, L. Stuppia, V. Gatta, S. Cecchin, G. Marceddu, M. Bertelli La Clinica Terapeutica, 2023
Omics sciences and precision medicine in sarcoma G. Bonetti, K. Donato, K. Dhuli, M. Gadler, S. Benedetti, G. Guerri, F. Cristofoli, D. Generali, C. A. Donofrio, M. Cominetti, A. Fioravanti, L. Riccio, A. Bernini, E. Fulcheri, D. Cavalca, L. Stuppia, L. Stuppia, V. Gatta, S. Cristoni, S. Cecchin, G. Marceddu, M. Bertelli La Clinica Terapeutica, 2023
Omics sciences and precision medicine in prostate cancer M. Medori, C. Micheletti, M. Gadler, S. Benedetti, G. Guerri, F. Cristofoli, D. Generali, C. A. Donofrio, M. Cominetti, A. Fioravanti, L. Riccio, A. Bernini, E. Fulcheri, A. E. Calogero, R. Cannarella, L. Stuppia, V. Gatta, S. Cecchin, G. Marceddu, M. Bertelli La Clinica Terapeutica, 2023
Omics sciences and precision medicine in colon cancer G. Madeo, G. Bonetti, M. Gadler, S. Benedetti, G. Guerri, F. Cristofoli, D. Generali, C. A. Donofrio, M. Cominetti, A. Fioravanti, L. Riccio, A. Bernini, E. Fulcheri, A. Iaconelli, B. Aquilanti, G. Matera, L. Stuppia, V. Gatta, S. Cecchin, G. Marceddu, M. Bertelli La Clinica Terapeutica, 2023
Genetic Analysis of Patients with Congenital Hypogonadotropic Hypogonadism: A Case Series Rossella Cannarella, Carmelo Gusmano, Rosita A. Condorelli, Andrea Bernini, Jurgen Kaftalli, Paolo Enrico Maltese, Stefano Paolacci, Astrit Dautaj, Giuseppe Marceddu, Matteo Bertelli, Sandro La Vignera, Aldo E. Calogero International Journal of Molecular Sciences, 2023
MAGI-Dock: a PyMOL companion to Autodock Vina J. Kaftalli, A. Bernini, G. Bonetti, S. Cristoni, G. Marceddu, M. Bertelli European Review for Medical and Pharmacological Sciences, 2023
Alteration of the Nucleotide Excision Repair (NER) Pathway in Soft Tissue Sarcoma Adriano Pasqui, Anna Boddi, Domenico Andrea Campanacci, Guido Scoccianti, Andrea Bernini, Daniela Grasso, Elisabetta Gambale, Federico Scolari, Ilaria Palchetti, Annarita Palomba, Sara Fancelli, Enrico Caliman, Lorenzo Antonuzzo, Serena Pillozzi International Journal of Molecular Sciences, 2022
Genetics of fat deposition G. Camilleri, A. K. Kiani, K. Herbst, J. Kaftalli, A. Bernini, K. Dhuli, E. Manara, G. Bonetti, L. Stuppia, S. Paolacci, A. Dautaj, M. Bertelli European Review for Medical and Pharmacological Sciences, 2021
Steroid-converting enzymes in human adipose tissues and fat deposition with a focus on AKR1C enzymes A. K. Kiani, M. Mor, A. Bernini, E. Fulcheri, S. Michelini, K. Herbst, F. Buffelli, J. Belgrado, J. Kaftalli, L. Stuppia, A. Dautaj, K. Dhuli, T. Guda, E. Manara, P. Maltese, P. Chiurazzi, S. Paolacci, M. R. Ceccarini, T. Beccari, M. Bertelli European Review for Medical and Pharmacological Sciences, 2021
AKUImg: A database of cartilage images of Alkaptonuria patients Alberto Rossi, Giorgia Giacomini, Vittoria Cicaloni, Silvia Galderisi, Maria Serena Milella, Andrea Bernini, Lia Millucci, Ottavia Spiga, Monica Bianchini, Annalisa Santucci Computers in Biology and Medicine, 2020
Nuclear magnetic resonance of amino acids, peptides, and proteins Amino Acids Peptides and Proteins in Organic Chemistry Volume 5 Analysis and Function of Amino Acids and Peptides, 2011
A structurally driven analysis of thiol reactivity in mammalian albumins Ottavia Spiga, Domenico Summa, Simone Cirri, Andrea Bernini, Vincenzo Venditti, Matteo De Chiara, Raffaella Priora, Simona Frosali, Antonios Margaritis, Danila Di Giuseppe, Paolo Di Simplicio, Neri Niccolai Biopolymers, 2011
Structurally driven selection of human hepatitis C virus mimotopes Ottavia Spiga, Maria G Padula, Maria Scarselli, Arianna Ciutti, Andrea Bernini, Vincenzo Venditti, Filippo Prischi, Chiara Falciani, Luisa Lozzi, Luisa Bracci, Piero E Valensin, Cinzia Caudai, Neri Niccolai Antiviral Therapy, 2006
NMR studies of protein hydration and TEMPOL accessibility Neri Niccolai, Ottavia Spiga, Andrea Bernini, Maria Scarselli, Arianna Ciutti, Irene Fiaschi, Stefano Chiellini, Henriette Molinari, Piero A Temussi Journal of Molecular Biology, 2003
NMR studies of protein surface accessibility Neri Niccolai, Arianna Ciutti, Ottavia Spiga, Maria Scarselli, Andrea Bernini, Luisa Bracci, Daniela Di Maro, Claudio Dalvit, Henriette Molinari, Gennaro Esposito, Piero A. Temussi Journal of Biological Chemistry, 2001
