Kateryna Pantiukh

@ut.ee

University of Tartu
Institute of Genomics, University of Tartu



                    

https://researchid.co/kateryna.pantiukh

EDUCATION

2009 – 2011 - Lomonosov Moscow State University (MSU) - Master's degree, Biotechnology
2009 – 2011 - Taras Shevchenko National University of Kyiv - Master's degree, Cytology, and histology
2005 – 2009 - Taras Shevchenko National University of Kyiv – Bachelor’s degree, Biology

RESEARCH INTERESTS

microbial genomics and metagenomics

7

Scopus Publications

123

Scholar Citations

6

Scholar h-index

4

Scholar i10-index

Scopus Publications


  • Erratum: The use of whole genome amplification for genomic evaluation of bovine embryos (Vavilovskii Zhurnal Genetiki i Selektsii (2019) 23:4 (489-495) DOI: 10.18699/VJ19.518)
    K. S. Pantiukh, I. V. Rukin, S. M. Portnov, A. Khatib, S. L. Panteleev, and A. M. Mazur
    Institute of Cytology and Genetics, SB RAS
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  • The use of whole genome amplification for genomic evaluation of bovine embryos
    K. S. Pantiukh, I. V. Rukin, S. V. Portnov, A. Khatib, S. V. Panteleev, and A. M. Mazur
    Institute of Cytology and Genetics, SB RAS
    The integration of high technologies into livestock production has been actively occurring in the last decade in the countries with a developed animal breeding. First of all, we are talking about reproductive technologies (IVF) and genomic technologies (general genomic evaluation of animal and genomic evaluation of breeding value). Combining reproductive and genomic technologies is a promising approach that allows receiving highquality breeding cattle in the shortest possible time. The basis of the proposed technology for accelerated reproduction of high-value breeding cattle is to obtain information about the genome of the embryo for genomic evaluation. The amount of genetic material that can be obtained for research is extremely limited, as it is necessary to preserve the viability of the embryo. The stage of the whole genome amplification was introduced to obtain a high quality of genetic material in a sufficient quantity. The main purpose of this work is to assess the possibility of using embryo biopsy specimens (bsp) for embryo genotyping using microarray chips and predicting the carrier status of lethal haplotypes at the embryo stage. We obtained 100 cattle embryos, of which 78 biopsy specimens were taken to analysis. For the biopsies obtained we performed the whole genome amplification. The quality and quantity of DNA for all the 78 samples after the whole genome amplification were satisfactory for further genotyping. The quality of the performed genotyping was satisfactory and allowed the assessment of lethal haplotype carriers (determining the sex of the animal and identification of the carrier status for sevenHolsteinlethal haplotypes). We tested 78 embryos. From the genotyping analysis, there was detected one carrier status for three lethal haplotypes, HH0 (Brachyspina), HH5, and HCD. The carrier status of HH0 and HH5 was confirmed by testing the casual mutation using PCR analysis. The carrier status for HCD has not been confirmed by casual mutation analysis. The situation in which an animal is an HCD carrier, but not the carrier of a casual mutation, can be explained. The putative ancestor of the haplotype is the bull HOCAN000000334489 WILLOWHOLME MARK ANTHONY (year of birth is 1975), but a casual mutation associated with this disease has arisen only in his descendant HOCAN000005457798 MAUGHLIN STORM (year of birth is 1991). The results obtained confirm the importance of testing the casual mutation in the animals that are carriers of lethal haplotypes according to the genotyping data.

  • Forecasting the Embryo productivity of donor cows on the basis of echographic characteristics of the ovaries
    A.V. Brigida, , V.I. Sorokin, S.N. Kovalchuk, K.S. Pantiukh, I.V. Rukin, K.A. Rozhin, , , ,et al.
    Sel'skokhozyaistvennaya Biologiya Editorial Office (SBEO), NPO
    The main problem restricting the wide use of reproductive biotechnology in animal husbandry is insufficiently developed methods for selection of donor cows for embryo transfer. The objective reason is the variability of the ovarian response to gonadotropins injections. Until now, there is no reliable information about possibility of forecasting the embryo productivity of donor cows before gonadotropin stimulation, which affects substantively the economic feasibility of embryo transfer as a method of accelerated cattle reproduction. We have applied the post-pressing analysis of ovaries echograms for forecasting the embryo productivity of donor cows on the basis of comparisons of quantitative and qualitative indicators of ovaries after induced superovulation and its’ echographic characteristics. We carried out morphofunctional study of ovaries in donor cows (n = 30) on day 10 of estrous cycle, before artificial insemination (estrus) and on day 7 of the inducted estrous cycle, immediately before the extraction of embryos using data on post-pressing ovarian morphometry. Animals were divided into three groups (I, II и III, n = 10 for each group) with yellow body length of 2.5 cm, 1.5-2.5 cm and 1.5 cm, respectively. Echographic visualization of the ovaries was performed using endorectal ultrasonography. Polyovulatory response of ovaries was induced with FSHsuper (Russia) injected eight times, with 12 h interval, at decreasing doses. The embryos were recovered on day 7 after artificial insemination. Optimal criteria for predicting the polyovulatory response of ovaries and the quantity of embryos were determined on the basis of the ovarian morphometry. Statistically significant differences with the control were assessed by the Student’s t-test. It was found that the average areas of the ovaries on the echograms were 7.9±0.94, 5.7±0.78 and 3.5±0.06 cm2 for group I, group II and group III, respectively. The area of the yellow body in group I averaged 4.5±1.21 cm2, was 2.08 cm2 higher (P  0.05) than in group II, and exceeded the corresponding parameter in group III by 3.43 cm2 (P  0.05). A comparative evaluation of the ratio of the yellow body areas to the ovaries area of each animal and on average along the groups showed that in group I with the ratio of 57.1±3.01 % the number of yellow bodies was 11.6±1.26 and the average yield of the embryos was 9.3±1.23 per animal. In group II with the ratio of the areas of yellow bodies and ovaries of 42.1±2.9 % the number of yellow bodies before embryos recovery was 5.7±1.24, and 4.6±1.01 embryos were recovered per procedure. The lowest embryo recovery (less than one embryo per procedure) was observed in group III with the relative area of yellow bodies of 30.2±2.56 % and the average number of the yellow bodies of 1.8±0.18. Comparison of the size of the yellow bodies before the induction of polyovulation and data characterizing the efficiency of induction of superovulation and recovery of embryos showed that in animals with a ratio of the areas of the yellow body and ovary more than 50 %, high response can be obtained resulting in 11.6±1.26 yellow bodies and 9.3±1.23 embryos per extraction.

  • Profiling of microRNAs in wild type and early flowering transgenic Chrysanthemum morifolium by deep sequencing
    O. A. Shulga, A. V. Nedoluzhko, A. V. Shchennikova, N. M. Gruzdeva, A. A. Shelenkov, F. S. Sharko, A. S. Sokolov, E. S. Pantiukh, S. M. Rastorguev, E. B. Prokhortchouk,et al.
    Springer Science and Business Media LLC

  • Report on noninvasive prenatal testing: Classical and alternative approaches [version 1; referees: 2 approved]
    Kateryna S. Pantiukh, Nikolay N. Chekanov, Igor V. Zaigrin, Alexei M. Zotov, Alexander M. Mazur, and Egor B. Prokhortchouk
    F1000 Research Ltd
    Concerns of traditional prenatal aneuploidy testing methods, such as low accuracy of noninvasive and health risks associated with invasive procedures, were overcome with the introduction of novel noninvasive methods based on genetics (NIPT). These were rapidly adopted into clinical practice in many countries after a series of successful trials of various independent submethods. Here we present results of own NIPT trial carried out in Moscow, Russia. 1012 samples were subjected to the method aimed at measuring chromosome coverage by massive parallel sequencing. Two alternative approaches are ascertained: one based on maternal/fetal differential methylation and another based on allelic difference. While the former failed to provide stable results, the latter was found to be promising and worthy of conducting a large-scale trial. One critical point in any NIPT approach is the determination of fetal cell-free DNA fraction, which dictates the reliability of obtained results for a given sample. We show that two different chromosome Y representation measures—by real-time PCR and by whole-genome massive parallel sequencing—are practically interchangeable (r=0.94). We also propose a novel method based on maternal/fetal allelic difference which is applicable in pregnancies with fetuses of either sex. Even in its pilot form it correlates well with chromosome Y coverage estimates (r=0.74) and can be further improved by increasing the number of polymorphisms.

  • New approaches to prenatal screening for chromosomal abnormalities: Maternal blood DNA screening
    Sukhikh G.T. Sukhikh, Trofimov D.Yu. Trofimov, Barkov I.Yu. Barkov, Donnikov A.E. Donnikov, Shubina E.S. Shubina, Korostin D.O. Korostin, Ekimov A.N. Ekimov, Goltsov A.Yu. Goltsov, Bakharev V.A. Bakharev, Karetnikova N.A. Karetnikova,et al.
    Bionika Media

RECENT SCHOLAR PUBLICATIONS

  • A history of repeated antibiotic usage leads to microbiota-dependent mucus defects
    KL Krigul, RH Feeney, S Wongkuna, O Aasmets, SM Holmberg, ...
    Gut microbes 16 (1), 2377570 2024

  • From Biobanking to Personalized Medicine: the journey of the Estonian Biobank
    L Milani, M Alver, S Laur, S Reisberg, T Haller, O Aasmets, E Abner, ...
    medRxiv, 2024.09. 22.24313964 2024

  • NO OBSERVABLE DIFFERENCES IN THE GUT MICROBIOME OF OSTEROARTHITIS PATIENTS
    K Bevc, L Malfertheiner, K Pantiukh, M Jaagura, A Havulinna, V Salomaa, ...
    Osteoarthritis and Cartilage 32, S60 2024

  • Human gut archaea collection from Estonian population
    K Pantiukh, E Org
    bioRxiv, 2024.12. 09.627479 2024

  • Metagenome-assembled genomes of Estonian Microbiome cohort reveal novel species and their links with prevalent diseases
    K Pantiukh, O Aasmets, KL Krigul, E Org
    bioRxiv 2024

  • The use of whole genome amplification for genomic evaluation of bovine embryos
    KS Pantiukh, IV Rukin, SM Portnov, A Khatib, SL Panteleev, AM Mazur
    Vavilov Journal of Genetics and Breeding 23 (4), 489-495 2019

  • Прогнозирование эмбриопродуктивности коров-доноров на основании эхографической характеристики яичников
    АВ Бригида, ВИ Сорокин, СН Ковальчук, КС Пантюх, ИВ Рукин, ...
    Сельскохозяйственная биология 53 (4), 753-761 2018

  • Profiling of microRNAs in wild type and early flowering transgenic Chrysanthemum morifolium by deep sequencing
    OA Shulga, AV Nedoluzhko, AV Shchennikova, NM Gruzdeva, ...
    Plant Cell, Tissue and Organ Culture (PCTOC) 128, 283-301 2017

  • New approaches to prenatal screening for chromosomal abnormalities: Maternal blood DNA screening
    GT Sukhikh, DY Trofimov, IY Barkov, AE Donnikov, ES Shubina, ...
    Obstetrics and Gynecology 8, 72-8 2016

  • Report on noninvasive prenatal testing: classical and alternative approaches
    KS Pantiukh, NN Chekanov, IV Zaigrin, AM Zotov, AM Mazur, ...
    F1000Research 5, 722 2016

  • Новые подходы к проведению пренатального скрининга хромосомной патологии: ДНК-скрининг по крови матери

    Акушерство и гинекология 8 2016

  • Blood extracellular DNA sequencing-based noninvasive prenatal diagnosis of fetal aneuploidies in a pregnant woman
    KS Pantyukh, ES Shubina
    Obstetrics and gynecology, 5-11 2015

  • Неинвазивная пренатальная диагностика анеуплоидий плода, основанная на секвенировании внеклеточной ДНК крови беременной женщины
    КС Пантюх, ЕС Шубина
    Акушерство и гинекология, 5-11 2015

  • Development a new method for noninvasive prenatal detection of fetal aneuploidy
    K Pantiukh, A Shanko, E Khrameeva, N Mospan
    Development 2, 23 2013

  • Геномная селекция-будущее в разведении животных
    ИВ Рукин, ЕС Пантюх, ДС Груздев
    Зоотехния, 8-9 2013

MOST CITED SCHOLAR PUBLICATIONS

  • Геномная селекция-будущее в разведении животных
    ИВ Рукин, ЕС Пантюх, ДС Груздев
    Зоотехния, 8-9 2013
    Citations: 43

  • Новые подходы к проведению пренатального скрининга хромосомной патологии: ДНК-скрининг по крови матери

    Акушерство и гинекология 8 2016
    Citations: 22

  • Profiling of microRNAs in wild type and early flowering transgenic Chrysanthemum morifolium by deep sequencing
    OA Shulga, AV Nedoluzhko, AV Shchennikova, NM Gruzdeva, ...
    Plant Cell, Tissue and Organ Culture (PCTOC) 128, 283-301 2017
    Citations: 11

  • Неинвазивная пренатальная диагностика анеуплоидий плода, основанная на секвенировании внеклеточной ДНК крови беременной женщины
    КС Пантюх, ЕС Шубина
    Акушерство и гинекология, 5-11 2015
    Citations: 11

  • Прогнозирование эмбриопродуктивности коров-доноров на основании эхографической характеристики яичников
    АВ Бригида, ВИ Сорокин, СН Ковальчук, КС Пантюх, ИВ Рукин, ...
    Сельскохозяйственная биология 53 (4), 753-761 2018
    Citations: 9

  • Report on noninvasive prenatal testing: classical and alternative approaches
    KS Pantiukh, NN Chekanov, IV Zaigrin, AM Zotov, AM Mazur, ...
    F1000Research 5, 722 2016
    Citations: 7

  • From Biobanking to Personalized Medicine: the journey of the Estonian Biobank
    L Milani, M Alver, S Laur, S Reisberg, T Haller, O Aasmets, E Abner, ...
    medRxiv, 2024.09. 22.24313964 2024
    Citations: 6

  • New approaches to prenatal screening for chromosomal abnormalities: Maternal blood DNA screening
    GT Sukhikh, DY Trofimov, IY Barkov, AE Donnikov, ES Shubina, ...
    Obstetrics and Gynecology 8, 72-8 2016
    Citations: 5

  • A history of repeated antibiotic usage leads to microbiota-dependent mucus defects
    KL Krigul, RH Feeney, S Wongkuna, O Aasmets, SM Holmberg, ...
    Gut microbes 16 (1), 2377570 2024
    Citations: 4

  • The use of whole genome amplification for genomic evaluation of bovine embryos
    KS Pantiukh, IV Rukin, SM Portnov, A Khatib, SL Panteleev, AM Mazur
    Vavilov Journal of Genetics and Breeding 23 (4), 489-495 2019
    Citations: 3

  • Blood extracellular DNA sequencing-based noninvasive prenatal diagnosis of fetal aneuploidies in a pregnant woman
    KS Pantyukh, ES Shubina
    Obstetrics and gynecology, 5-11 2015
    Citations: 2

Publications

Pantiukh KS, Chekanov NN, Zaigrin IV, Zotov AM, Mazur AM, Prokhortchouk EB. Report on noninvasive prenatal testing: classical and alternative approaches. F1000Res. 2016 Apr 22;5:722.
Pantiukh K., Shanko A., Khrameeva E., Mospan N. Development a new method for noninvasive prenatal detection of fetal aneuploidy// Prenat Diagn 2013; 33(Suppl. 1): P-53.
Patent 2585519 (27.05.2016) A method for selecting target DNA regions