Gedunin attenuates streptozotocin-induced diabetic hepatopathy in rats Suchismita Mazumdar, Thankamani M Marar, Jyoti M Patki Asian Pacific Journal of Tropical Biomedicine, 2024 Objective: To examine the hepatoprotective effects of gedunin in streptozotocin (STZ)-induced diabetic rats. Methods: Rats were divided into 4 groups: control, STZ, gedunin, and STZ+gedunin. Biochemical parameters for liver function and liver histology were studied. The molecular interaction of gedunin with the liver glucose transporters GLUT2 and SGLT1 was examined using AutoDock Vina. Results: Gedunin attenuated STZ-induced increase in the levels of aspartate transaminase, alanine transaminase, alkaline phosphatase, lactate dehydrogenase and gamma-glutamyl transferase in the serum and liver tissue, reduced lipid peroxidation, and enhanced antioxidant activity. Histopathological studies showed considerable restoration of liver architecture in gedunin-treated diabetic rats. In silico studies revealed stable binding of gedunin with GLUT2 and SGLT1. Conclusions: Gedunin exerts hepatoprotective effects in STZ-induced diabetic rats by reducing liver enzymatic activities and oxidative stress. Further studies are warranted to verify the mechanism of its hepatoprotective action.
Biofilms: Formation, Regulation and Detection Kanchanlata Tungare, Sneha P. Nair, D. Sharanya, Ashish Cyril, Santhosh Jathar, Mustansir Bhori, Thankamani Marar, Renitta Jobby Biofilm Associated Antimicrobial Resistance and Its Recovery, 2023 Biofilms are specialized diverse species of bacteria and fungi that adhere together due to the production of extra polymeric substances, making them viscoelastic in order to survive hostile environments. It facilitates the bacteria’s ability to persist in the environment, thereby playing a fundamental role in its epidemic cycle. The proliferation of the biofilm is a complex process and is regulated by several factors. When adhered together to a surface, these microorganisms undergo processes such as exchange of substrates, distribution of metabolites, and eviction of toxic end products. These processes make them resistant to both the immune defense mechanisms of the host and antibiotic therapy, thus emerging as a major public health hazard. The recalcitrant ability of the pathogenic biofilm to persist even in a high concentration of antimicrobial compounds makes treatment of them a gruesome task. This chapter focuses on the stepwise formation of biofilms and the metabolomic regulation that microorganisms undergo to facilitate the formation of biofilms. This chapter also encompasses different detection techniques that mainly rely on two attributes, the common phenotype of the organisms which display the characteristic features of slime production and the genotype which leads to their production.
A review on Instant Controlled Pressure Drop Technology (DIC) associated with Drying technology and effect on quality characteristics Riddhi Rane, Thankamani Marar, Sachin K Sonawane, Ashish Dabade Food Chemistry Advances, 2022 Instant Controlled Pressure Drop Technology (DIC) was first introduced as a drying technology but later applied to other processes such as developing puffed snacks, texture enhancing, deodorizing, etc. DIC can replace multiple conventional drying methods to obtain higher quality dried products with improved sensory and texture properties. It can also be used as a pretreatment or in conjunction with different drying processes. This review focuses on the DIC parameters which affect the product quality, working equipment, and types of DIC equipment illustrated, followed by combining DIC with the other technologies, including dehydro-freezing, freezing, freeze-drying, and osmotic pretreatments moisture equilibration process (MEP). Additionally, deodorization by DIC is also discussed. The importance of DIC in extraction, quality variations produced by DIC, and DIC's usage of waste products are all explored.
Comparative study of ice-cream cones developed from refined wheat, ragi, buckwheat, bajra, amaranth, and composite flour Roopal Mhatre, Marar Thankamani, Sachin K Sonawane, Pravin Bhushette Measurement Food, 2022 This study explored refined wheat flour, ragi, buckwheat, bajra, amaranth, and composite flour without refined wheat flour to develop an ice cream cone. For forming a composite flour rolled ice cream cone, refined wheat flour was wholly substituted by a mixture of 40% ragi, 35% buckwheat, 15% bajra, 10% amaranth flour. The influence of egg white incorporation in the preparation of ice-cream cones on yield%, baking time, and ice cream holding time was investigated. The composite flour cone stayed crisp and retained ice cream for 37 min and 35 s in functioning. The eggless composite flour cone received better appearance, flavor, and taste ratings than the refined wheat flour cones, but the wheat cones were higher for texture.
Functional relevance of Gedunin as a bona fide ligand of NADPH oxidase 5 and ROS scavenger: An in silico and in vitro assessment in a hyperglycemic RBC model Suchismita Mazumdar, Thankamani Marar, Shine Devarajan, Jyoti Patki Biochemistry and Biophysics Reports, 2021 Clinical evidence suggests that type 2 diabetes therapy can greatly benefit from the suppression of reactive oxygen species generation and the activation or restoration of cellular antioxidant mechanisms. In human, NADPH oxidase (NOX) is the main producer of reactive oxygen species (ROS) that supress the activity of endogenous antioxidant enzymes. In the present study, the antioxidant potential of Gedunin was studied. In silico findings reveal its strong binding affinity with NOX5 C terminal HSP90 binding site that disrupts NOX5 stability and its ability to generate ROS, leading to restoration antioxidant enzymes activities. It was found that Gedunin suppressed hyperglycaemia induced oxidative stress in an in vitro RBC model and markedly reversed glucose induced changes including haemoglobin glycosylation and lipid peroxidation. A significant restoration of activities of cellular antioxidant enzymes; superoxide dismutase, catalase and glutathione peroxidase in the presence of Gedunin revealed its ability to reduce oxidative stress. These results substantiated Gedunin as a bona fide inhibitor of human NOX5 and a ROS scavenging antioxidant with promising therapeutic attributes including its natural origin and inhibition of multiple diabetic targets.
In-vitro Biocompatibility Studies of Mint Oil-Vitamin D Nanoparticles International Journal of Nanoscience and Nanotechnology, 2021
Vetting and comparative analysis of antioxidants and phytochemicals from methanolic, aqueous and a popular commercial fruit juice (Noni) Morinda citrifolia L. Research Journal of Pharmaceutical Biological and Chemical Sciences, 2015
Inhibitory effect of Allium sativum on low-density lipoprotein (LDL) oxidation induced by CuSO4 In-Vitro Research Journal of Pharmaceutical Biological and Chemical Sciences, 2015
Impact of antioxidant supplementation on toxicity of methotrexate: An in vitro study on erythrocytes using vitamin E Asian Journal of Pharmaceutical and Clinical Research, 2015
Comparative evaluation of various total antioxidant capacity assays applied to phytochemical compounds of indian culinary spices International Food Research Journal, 2013
Bougainvillea spectabilis, a good source of antioxidant phytochemicals Research Journal of Pharmaceutical Biological and Chemical Sciences, 2012
Comparative analysis of phytochemical profile and antioxidant activity of some Indian culinary herbs Research Journal of Pharmaceutical Biological and Chemical Sciences, 2012
