Andini

RESEARCH INTERESTS

chemistry
analytical chemistry
food analysist
pharmacy analysist

Scopus Publications

Sustainable honey-mediated synthesis of oligochitosan-functionalized Ca(OH)₂ nanoparticles with enhanced antibacterial and biocompatible properties
Saidun Fiddaroini, Ardini Nurul Janah, Budi Mulyati, Ahmad Luthfi Fahmi, Yosep Yuswanto Tri Ananda, Andini, Nike Fitayatul Khusnah, Moh. Farid Rahman, Akhmad Sabarudin
Nano Trends, 2026
• Honey-assisted green synthesis enables controlled formation of Ca(OH)₂ nanoparticles • Oligochitosan functionalization improves stability and surface bioactivity • Nanocomposites show enhanced antibacterial activity against Gram+ and Gram− bacteria • Molecular docking reveals multi-target interactions with bacterial proteins • Oligochitosan coating reduces toxicity and improves biocompatibility Antimicrobial resistance (AMR) remains a critical global challenge, particularly against Staphylococcus aureus and Pseudomonas aeruginosa. This study presents a green synthesis of Ca(OH)₂ nanoparticles using cottonwood honey ( Ceiba pentandra ) as a direct reducing–stabilizing agent. The novelty lies in eliminating extraction steps and combining honey-mediated synthesis with oligochitosan coating to enhance stability and antibacterial performance. Ca(OH)₂ nanoparticles were synthesized via a honey-mediated approach and subsequently functionalized with oligochitosan. Structural, morphological, and colloidal properties were characterized using UV–Vis, FTIR, FESEM–EDX, TEM–SAED, XRD, DLS, and TGA, while antibacterial activity was evaluated using the broth microdilution method. FTIR confirmed successful surface functionalization, and TEM revealed relatively uniform nanoparticles (20–30 nm) with improved dispersion after coating. DLS analysis showed increased hydrodynamic diameter and a shift in zeta potential, indicating the formation of a stable polymeric shell. The antibacterial activity significantly increased after coating, with enhancement efficiencies of 443% against S. aureus and 545% against P. aeruginosa, demonstrating a strong synergistic effect. In silico molecular docking supported a multi-target antibacterial mechanism through strong interactions with membrane- and cell-wall–associated proteins. Biocompatibility assessment using the brine shrimp lethality test showed higher LC₅₀ values for coated nanoparticles, indicating reduced toxicity. This work demonstrates that cottonwood honey–derived Ca(OH)₂–oligochitosan nanocomposites present a promising, eco-friendly, and biocompatible antibacterial platform for combating drug-resistant pathogens.
Honey-mediated green synthesis and systematic optimization of Ni(OH)₂ nanoparticles with oligochitosan surface modification: Structural characterization, enhanced stability, and antibacterial biocompatibility
Saidun Fiddaroini, Berlian Zetta Batari, Budi Mulyati, Ahmad Luthfi Fahmi, Yosep Yuswanto Tri Ananda, Andini, Moh. Farid Rahman, Akhmad Sabarudin
Nano Structures and Nano Objects, 2026
Printed low-cost microfluidic paper-based analytical devices for quantitative detection of vitamin c in fruits
Andini, Ulfa Andayani, Anneke, Meiria Istiana Sari, Akhmad Sabarudin
Iop Conference Series Materials Science and Engineering, 2019
Microfluidic paper-based analytical device has been fabricated and subsequently applied to determine the Vitamin C level of the non-seasonal fruit i.e pineapples, oranges, guavas, and apples in Indonesia. µPAD was prepared by creating a hydrophobic barrier on Whatman Chromatography paper No. 1 in a certain pattern printed using Xerox Colorcube 8580 DN-2. 0.05 µl of 2,6-Dichlorophenolindophenol was dispersed into a detection area on the µPAD devices prior to the addition of 0.2 µl of fruits juice samples. The barrier allowed the fruits juice to flow and alter the color of the reagents through a redox reaction. Vitamin C in fruit juice altered the color of 2.6-Dichlorophenolindophenol reagent into a pinkish color. The changing of the reagent color was then processed using Image J software to determine the RGB intensity level. The results showed that the average amounts of Vitamin C in pineapples, oranges, guavas, and apples are 0.05%, 0.05%, 0.08%, and 0.04% respectively.
Microfluidic paper based analytical device (pads) for analysis of benzoat acid in packaged beverages
M I Sari, Ulfa Andayani, Anneke, Andini, Akhmad Sabarudin
Iop Conference Series Materials Science and Engineering, 2019
Microfluidic Paper-based Analytical Devices (μPADs) are a simple analytical platform that satisfy combination of low cost, portability, and ease-to-use with selectivity, sensitivity, and accuracy. In this experiment, the μPADs devices are prepared using a chromatographic paper and designed at appropriate pattern prior to printing by mean of a solid ink (wax) printer to produce hydrophobic barriers and hydrophilic channels. Then, the μPADs are heated at 120°C for 3 min to allow a wax penetrates in the both side of a paper. The resulted μPADs are used for the detection of benzoic acid through alkalimetry principle. For this purpose, the devices are implanted with 5M NaOH in the detection zone and a 2% phenolphthalein indicator in the sample zone before dried in open air. When the devices have dried, the μPADs are ready to be used to detect benzoic acid. To avoid color interferences, the samples are distilled before introduced to the sample zone for quantitative detection. The result soft the color changes can be immediately seen by naked eye, and their intensities are quantified by the Image J software. Excellent linearity is achieved in the range of benzoic acid concentration of 20-120 ppm with the linear equation of Y = 0.0102 x + 0.0077 and the correlation coefficient (R2) of 0.9999. The optimized μPADs devices are successfully applied to the quantitative analysis of benzoic acid in the 5 kinds of commercially packaged beverages. There is no significantly different of the analytical results obtained by the µPADs in comparison to the spectrophotometric method. Overall, the results obtained in this study indicate that the μPADs devices are a reliable tool for high throughout and on-site determination of benzoic acid content in commercial drinks.
Developing a mickey-mouse-designed microfluidic paper-based analytical device (μpad) to determine the antioxidant activity of green tea
Na’ilah Fauziyah, Andini, Anneke, Ika Oktavia, Meiria Istiana Sari, Hermin Sulistyarti, Akhmad Sabarudin
Iop Conference Series Materials Science and Engineering, 2019
The determination of the antioxidant activity in green tea using FRAP essays developed in a Microfluidic Paper-Based Analytical Device (μPADs) was observed. μPAD was prepared on the chromatographic paper with a suitable pattern and then printed using a solid ink printer. The solid printing was intended to obtain a hydrophobic barrier and a hydrophilic channel on the chromatographic paper. A preliminary study was done to determine the optimum time and temperature used on the penetration of obtaining the hydrophobic barrier to avoid leakage in the channel. Optimization of temperature and time was calculated by the average velocity, the optimum condition was obtained at 120 °C for 90 seconds with an average speed of 0.1 mm.s−1. The green tea samples were prepared by extracting its active compound using demineralized water at 25 °C and 90 °C with 2 hours of immersion time. For the measurement of antioxidant activities, the analysis was carried out by placing 0.5 μl in the detection zone and 5 μl samples into the sample zone μPAD. Then, the color reaction product, which propagates from the sample zone to the detection zone, is processed by Image J software to measure color intensity in CMYK mode. Extracts of green tea samples at 25 °C and 90 °C has a significant difference in antioxidant activity. These results indicate that the method developed in this work can be used as an alternative method for analyzing antioxidant activity in green tea extract. The results of the average amount of antioxidant activity in green tea samples were shown with Fe2+ concentration. High-speed detection, low cost, high accuracy, and ease of use can be attributed to the advantages of our μPAD method.

RECENT SCHOLAR PUBLICATIONS

Microfluidic Paper-based Analytical Devices (μPADs) Untuk Analisis Asam Benzoat Pada Minuman Kemasan
M Istiana Sari
Universitas Brawijaya , 2019
2019
Microfluidic Paper based Analytical Device (µpads) for Analysis of Benzoat Acid in Packaged Beverages
MI Sari, U Andayani, Anneke, Andini, A Sabarudin
IOP Conference Series: Materials Science and Engineering 546 (3), 032028 , 2019
2019
Citations: 2
Developing a mickey-mouse-designed microfluidic paper-based analytical device (μpad) to determine the antioxidant activity of green tea
N Fauziyah, Andini, Anneke, I Oktavia, MI Sari, H Sulistyarti, A Sabarudin
IOP Conference Series: Materials Science and Engineering 546 (3), 032007 , 2019
2019
Citations: 4
Printed Low-Cost Microfluidic Paper-based Analytical Devices for Quantitative Detection of Vitamin C in Fruits
Andini, U Andayani, Anneke, MI Sari, A Sabarudin
IOP Conference Series: Materials Science and Engineering 546 (3), 032002 , 2019
2019
Citations: 4
Enzim Î±-Amilase Inhibitor Pada Ekstrak Air Kacang Merah (Phaseolus vulgaris L.) Untuk Penanggulangan Diabetes Melitus
NAK Wardani
Jurnal Ilmu Pangan dan Hasil Pertanian 1 (2), 50-59 , 2017
2017
Citations: 23

MOST CITED SCHOLAR PUBLICATIONS

Enzim Î±-Amilase Inhibitor Pada Ekstrak Air Kacang Merah (Phaseolus vulgaris L.) Untuk Penanggulangan Diabetes Melitus
NAK Wardani
Jurnal Ilmu Pangan dan Hasil Pertanian 1 (2), 50-59 , 2017
2017
Citations: 23
Developing a mickey-mouse-designed microfluidic paper-based analytical device (μpad) to determine the antioxidant activity of green tea
N Fauziyah, Andini, Anneke, I Oktavia, MI Sari, H Sulistyarti, A Sabarudin
IOP Conference Series: Materials Science and Engineering 546 (3), 032007 , 2019
2019
Citations: 4
Printed Low-Cost Microfluidic Paper-based Analytical Devices for Quantitative Detection of Vitamin C in Fruits
Andini, U Andayani, Anneke, MI Sari, A Sabarudin
IOP Conference Series: Materials Science and Engineering 546 (3), 032002 , 2019
2019
Citations: 4
Microfluidic Paper based Analytical Device (µpads) for Analysis of Benzoat Acid in Packaged Beverages
MI Sari, U Andayani, Anneke, Andini, A Sabarudin
IOP Conference Series: Materials Science and Engineering 546 (3), 032028 , 2019
2019
Citations: 2
Microfluidic Paper-based Analytical Devices (μPADs) Untuk Analisis Asam Benzoat Pada Minuman Kemasan
M Istiana Sari
Universitas Brawijaya , 2019
2019