The Effect of Human Adipose Stem Cell-Conditioned Medium (hASC-CM) in Photoaged Skin Winawati Eka Putri, Meidyta Sinantryana Widyaswari, C. Prakoeswa, David Sajid Muhammad, Deny Febriwijaya Romadhani, Nadia Nisaussholihah Journal of Stem Cells and Regenerative Medicine, 2024 Background: Ultraviolet (UV) exposure causes direct and indirect damages to skin structures. Human adipose stem cell-conditioned medium (hASC-CM) is a collection of several soluble factors, such as cytokines, chemokines, and Growth Factors (GF), secreted by almost all living cells in the extracellular space which support wound healing and skin rejuvenation. Objective: To determine the effects of human adipose stem cell-conditioned medium (hASC-CM) in photoaged skin and evaluate photoaging improvement after treatment. Methods: An experimental randomized controlled trial was performed, involving 64 photoaged subjects at the Dermato-Venereology Outpatient Clinic of Jemursari Islamic Hospital Surabaya from March to June 2022. The subjects were divided into the hASC-CM group and vehiculum (control) group. Patients' transepidermal water loss value, skin tone, and Glogau score in weeks 0, 4, and 8 were evaluated. The data were then analyzed using Mann-Whitney test (p<0.05). Results: All subjects had Glogau scale of III (89.6%) with mean ± SD (3.121 ± 0.329). hASC-CM group showed higher improvements in the pore, wrinkle, spot polarized, spot UV parameters and skin tone compared to vehiculum group(p<0.05). Conclusions: hASC-CM effectively improved all parameters observed. The limitation of this research was on the lack of long-term follow-up after treatment. This research had received an ethical permit from The Ethical Committee Board of Jemursari Islamic Hospital Surabaya under letter 006/KEPK-RSISJS/II/2022.
Comparison of the histological features in the photoaged animal model Winawati Eka Putri, Meidyta Sinantryana Widyaswari, Rizki Amalia, David Sajid Muhammad, Nadia Nisaussholihah, et al. Bali Medical Journal, 2023 Background: Photoaging is a type of aging primarily caused by exposure to radiation, such as Ultraviolet B (UVB). This radiation penetrates the upper epidermis and papillary dermis leading to sunburn, tanning, photoaging, and photocarcinogenesis. Its light is a significant cause of direct deoxyribonucleic acid (DNA) damage. Furthermore, it induces transcription factors, such as NF- and other pro-inflammatory cytokines, the expression of matrix metalloproteinase (MMP), and generates reactive oxygen species (ROS). Therefore, this study aimed to determine the differences in the histologic features of the photoaged animal models. Methods: There were three groups, including P1, P2, and P3. Group P1 consists of male Wistar rats (Rattus norvegicus), aged 10-12 weeks, with average body weight between 100-150 grams. Group P2 consists of C57BL aged 5-6 weeks with an average weight of 50-75 grams. P3 comprises BABL/c mice aged 5-6 weeks with an average weight of 50-75 grams. They were photoaged for 6 weeks using UV lamps (Ultraviolet B Broadband TL lamps. Phillips TL 20W/01 RS) with a 290-315 nm wavelength. Results: The results showed a significant difference in epidermal thickness, dermal thickness, and sunburn cell (SBC) between groups after exposure to UVB radiation (p<0.05). Meanwhile, there was no significant difference in their blood vessels (p>0.05). Conclusion: The exposure to UVB for six weeks affects epidermal and dermal thickness, amount of SBC, and blood vessels in the photoaged animal model.
The effect of long exposure to uvb rays on histological features of wistar rats (Rattus norvegicus) in photoaging model Nadia Hidayati, Noor Aulia Hatikhah, Winawati Eka Putri, Meidyta Sinantryana Widyaswari, David Sajid Muhammad, et al. Bali Medical Journal, 2023 Introduction: Indonesia is a tropical country with exposure to UV rays from the sun throughout the year, so the Indonesian population is very vulnerable to skin aging, especially extrinsic skin aging due to prolonged exposure to UV rays. This study aimed to determine the effect of long exposure to UVB rays on the thickness of the epidermis, the thickness of the dermis, and the number of blood vessels and sunburn cells in (Rattus norvegicus) Wistar strain. Methods: This research was a post-test-only control group design using 27 rats which were divided into 3 groups, namely: control group K was untreated rats, and treatment groups P1 and P2 were rats exposed to UVB radiation for 3 weeks and 6 respectively exposure weeks. Test the effect on the study using the One-Way ANOVA test and Tukey’s Post Hoc Test. Results: The results indicated an increase in epidermal thickness of groups P1 (44.87 μm) and P2 (56.90 μm) (significant p<0.05). A decrease in dermis thickness occurred in groups P1 (726.91 μm) and P2 (559.40 μm) (significant p<0.05). In the photoaging area, there was an increased amount of sunburn cells in group P1 (6.40) and P2 (11.40) (significant p<0.05). There was a difference in the number of blood vessels between groups K and P2 and groups P1 and P2 (p<0.05). Conclusion: There was an effect of long exposure to UVB rays on histology in rats (Rattus norvegicus) of the photoaging model including the thickness of the epidermis layer and dermis layer, the number of blood vessels, and sunburn cells.
Effect of secretome of adipose stem cell (ASC) in photoaging skin Winawati Eka Putri, Anang Endaryanto, Damayanti Tinduh, Fedik Rantam, Hari Basuki Notobroto, Cita Rosita Sigit Prakoeswa Bali Medical Journal, 2022 Introduction: UV light is the principal cause of photoaging, a kind of aging. The problem arises because it is less enjoyable. Utilizing the secretome of adipose stem cells (ASC), which is rich in growth factors, could be a novel anti-photoaging therapy. The secretome of ASCs has been extensively examined for their role in wound healing, but their function in photoaging has just lately been investigated. This study looked at changes in epidermal and dermal thickness, expression of MMP-1 and TIMP-1, as well as dermal collagen density to assess the influence of the secretome of ASC in photoaging treatment. Methods: 28 male Wistar rats, 10-12 weeks old, were randomly assigned to one week of acclimation in each of four groups: UV-irradiated group with topical secretome of ASC (Group P1); UV-irradiated group with topical vehicle only (Group P2); only topical secretome of ASC, non-UV-irradiated (Group P3); and non-UV-irradiated and no topical treatment (Group P4) (Group P0). Prior to treating the rats, the UV exposure was administered for six weeks. Then, for a period of four weeks, the secretome ASC was administered as a treatment. Results: The epidermal and dermal thickness were significantly improved after four weeks treatment compared to UV irradiated with topical vehicle only group (p<0.05). The expression of TIMP-1 and dermal collagen density were significantly increased (p<0.05). Conclusion: Secretome of ASC may be a solution for photoaging skin.
Correlation between transepidermal water loss with proinflammatory cytokines in non-aging and aging group Journal of Pakistan Association of Dermatologists, 2021
Growth factors of hypoxia freeze-dried adipose stem cell-conditioned medium (FD ASC-CM) and fresh adipose stem cell conditioned-medium (FR ASC-CM): A comparative study Winawati Eka Putri, Anang Endaryanto, Damayanti Tinduh, Fedik Rantam, Medhi Denisa Alinda, Cita Rosita Sigit Prakoeswa Bali Medical Journal, 2021 Growth factors of hypoxia Freeze-Dried Adipose Stem CellConditioned Medium (FD ASC-CM) and Fresh Adipose Stem Cell Conditioned-Medium (FR ASC-CM): a comparative study Winawati Eka Putri1,2, Anang Endaryanto3, Damayanti Tinduh4, Fedik Rantam5, Medhi Denisa Alinda6, Cita Rosita Sigit Prakoeswa6* Background: Adipose Stem Cells (ASC) are multipotent stem cells which mechanism of action is mainly secretion of various paracrine molecules known as secretome or conditioned medium (CM). Adipose Stem Cell-Conditioned Medium (ASC-CM) collects several soluble factors, such as cytokines, chemokines, and Growth Factors (GF) secreted by almost all types of living cells in the extracellular space. The freeze-dried (FD) form of ASC-CM can reduce the instability of the fresh (FR) form of ASCCM. This study aims to compare the GF levels between FD ASC-CM and FR ASC-CM as a comparative study. Methods: ASC-CM was prepared from human adipose tissue. After we got the FR ASC-CM, some of them were changed into an FD form. We measured the several GF levels using ELISA methods in ng/L. Data were presented in mean and standard deviation as well as analyzed using SPSS version 25 for Windows. Results: The average levels of EGF in both FD ASC-CM and FR ASC-CM were 256.80±9.70 ng/L and 290.20±53.20 ng/L, followed by TGF-β (564.20±12.70 ng/L and 633.10±11.40 ng/L), PDGF (2.90±0.19 ng/L and 2.90±0.20 ng/L), and VEGF (711.10±21.70 ng/L and 788.70±204.80 ng/L) respectively. There was a statistically significant difference in TGF-β levels between FD ASC-CM and FR ASC-CM (p=0.002). However, there was no a significant difference in EGF, PDGF, and VEGF levels between FD ASC-CM and FR ASC-CM (p>0.05) Conclusion: There was a statistically significant difference between FD and FR ASC-CM only in TGF-β levels.
Skin barrier before and after topical adipose stem cell-conditioned medium (Asc-cm) treatment in photoaging Winawati Eka Putri, Anang Endaryanto, Damayanti Tinduh, Fedik Rantam, Hari Basuki Notobroto, Cita Rosita Sigit Prakoeswa Bali Medical Journal, 2021 Skin barrier before and after topical Adipose Stem Cell-Conditioned Medium (ASC-CM) treatment in photoaging Winawati Eka Putri1,2, Anang Endaryanto3, Damayanti Tinduh4, Fedik Rantam5, Hari Basuki Notobroto6, Cita Rosita Sigit Prakoeswa6* Background: Photoaging is a type of aging mainly caused by ultraviolet irradiation. The skin barrier is affected in photoaging and it will result in coarseness, roughness and dry skin. It is probably because Ultraviolet (UV) affects the extracellular matrix in the skin. Adipose Stem Cell-Conditioned Medium (ASC-CM), which contains many growth factors, becomes a potential agent as a novel anti-photoaging treatment by repairing the skin barrier. This study aims to evaluate the skin barrier function before and after treatment with ASC-CM in photoaging skin by evaluating changes in Transepidermal Water Loss (TEWL) and skinfold thickness. Methods: A true experimental study was conducted among 28 male Wistar rats aged 10-12 weeks. They were acclimated for 1 week and randomly divided into 4 groups: UV irradiated group with topical ASC-CM (Group P1); UV irradiated group with topical vehicle only (Group P2); only topical ASC-CM, non-UV irradiated (Group P3), and non-UV irradiated and no topical treatment (Group P0). The ASC-CM was given twice a day for four weeks and the total irradiance dose for 6 weeks was 4,2 J/ cm2. Data were analyzed using SPSS version 20 for Windows. Results: There was a statistically significant difference of TEWL between pre-and post-treatment in group P3 (p=0.043) and P0 (p=0.018). In addition, a statistically significant difference in skin thickness was also found between pre-and posttreatment in group P2 (p=0.005) and P3 (p=0.003). There was a statistically significant difference in skin thickness posttreatment (p=0.000); however, there was no statistically significant difference of TEWL post-treatment by One-Way ANOVA test (p=0.138). Conclusion: ASC-CM could be a potential option for photoaging skin based on the TEWL and skin thickness evaluation. However, a longer study period was needed to know the further effect of ASC-CM.
Leprosy and Immune System: An Insight into the Innate Immune System Indian Journal of Leprosy, 2021
RECENT SCHOLAR PUBLICATIONS
The Effect Of Ethanolic Extract Of Katuk Leaf (Sauropus Androgynus) On Malondialdehyde (MDA) Levels In Ovalbumin-Induced Atopic Dermatitis Model Mice HSD Izzaty, WE Putri, S Syifak Asian Journal of Healthy and Science 4 (10), 353-364 , 2025 2025
The Pro-Apoptotic Effect of Diallyl Trisulfide (DATS) on MDA-MB-231 Breast Cancer Cells ESD Ailany, L Veterini, WE Putri, DW Kurniasari International Islamic Medical Journal 7 (1), 69-77 , 2025 2025
Discovering the Miracle of Stem Cells A Noviantari, JA Pawitan, IH Dilogo, S Hendrawan, J Lheman, DV Lukas, ... Penerbit BRIN , 2025 2025
Peningkatan Pengetahuan Penggunaan Tabir Surya untuk Mencegah Penuaan Ekstrinsik di RS Islam Jemursari Surabaya WE Putri, DS Muhammad, A Dinana, SE Ariefianto BERNAS: Jurnal Pengabdian Kepada Masyarakat 6 (1), 537-541 , 2025 2025 Citations: 1
The Effect of Sunscreen Use on The Incidence of Melasma in Police Officers: Meta-Analysis and Systemic Review IN Putri, AP Putra, N Juniano, E Budi, WE Putri, R Mayangsari, ... International Islamic Medical Journal 6 (2), 12-24 , 2024 2024 Citations: 2
The Effect of Human Adipose Stem Cell-Conditioned Medium (hASC-CM) in Photoaged Skin WE Putri, MS Widyaswari, CRS Prakoeswa, DS Muhammad, ... Journal of Stem Cells & Regenerative Medicine 20 (2), 47 , 2024 2024 Citations: 2
Pencegahan dan Penanganan Kasus Pedikulosis Kapitis di Lingkungan Pondok Pesantren I Farindra, WE Rusdi, WE Putri, VSP Saffanah, RY Nailuvar, SNW Putri, ... Jurnal Pemberdayaan Masyarakat Universitas Al Azhar Indonesia 6 (3), 190 , 2024 2024 Citations: 4
The relationship between fast food, sleep patterns, and facial hygiene with the severity of Acne vulgaris WE Putri, NF Hilda, FR Kusumah, AA Yulian, M Ulfa, MS Widyaswari, ... Biomolecular and Health Science Journal 7 (2), 88-95 , 2024 2024 Citations: 4
Penguatan Kelas Ibu pasca salin dan Menyusui sebagai upaya Pemenuhan Nutrisi R Amalia, A Syafiuddin, Y Windarti, U Laili, WE Putri BERNAS: Jurnal Pengabdian Kepada Masyarakat 5 (1), 706-710 , 2024 2024
Efektivitas Ekstrak Rimpang Jahe Merah (Zingiber Officinale Varietas Rubrum) Terhadap Pertumbuhan Malassezia Furfur Pada Pitiriasis Versikolor B Wibowo, MS Widyaswari, WE Putri PREPOTIF: JURNAL KESEHATAN MASYARAKAT Учредители: Universitas Pahlawan … , 2024 2024 Citations: 2
Comparison of the histological features in the photoaged animal model WE Putri, MS Widyaswari, R Amalia, DS Muhammad, N Nisaussholihah, ... Bali Medical Journal 12 (3), 3064-3069 , 2023 2023 Citations: 3
The effect of long exposure to uvb rays on histological features of wistar rats (Rattus norvegicus) in photoaging model N Hidayati, NA Hatikhah, WE Putri, MS Widyaswari, DS Muhammad, ... Bali Medical Journal 12 (3), 3078-3083 , 2023 2023 Citations: 4
Efficacy of ethanol extract of water clover (Marsilea crenata) leaves on estradiol levels in wistar rats post ovarectomy skin aging model DS Devi, WE Putri, R Amalia YOUNG SCHOLAR SYMPOSIUM ON SCIENCE AND MATHEMATICS EDUCATION, AND … , 2023 2023
Dasar Ilmu Kesehatan Kulit dan Kelamin WE Putri Unusa Press 1, 51 , 2023 2023
Korespondensi AIP Conference Proceedings-Efficacy of Ethanol Extract of Water Clover (Marsilea crenata) Leaves on Estradiol Levels in Wistar Rats Post Ovarectomy Skin Aging Model DS Devi, WE Putri, R Amalia AIP Publishing , 2023 2023
Skin response to lipopolysaccharide-induced sepsis based on histology M Ulfa, HM Salim, WE Putri, I Farindra Bali Medical Journal (Bali MedJ) 12 (3), 3251-3253 , 2023 2023
Penerapan Kamar Sehat untuk Mengurangi Kejadian Skabies di PP. Al Fitrah As Salafiyah Surabaya WE Putri, R Anggraini, EP Rahayu, Y Agnovianto Prosiding Seminar Nasional Pengabdian Kepada Masyarakat 3 (1), 273-277 , 2023 2023 Citations: 2
Akne Vulgaris dan Tatalaksana: Untuk Mahasiswa Bidang Kesehatan WE Putri, MS Widyaswari, M ULFA Unusa Press , 2023 2023
Pendampingan Kader dalam Sosialisasi Pengelolaan Sampah pada Santri di Pondok Pesantren Hidayatullah Al Muhajirin Madura MS Widyaswari, WE Putri, M Ulfa, M Shafly, DI Salsabila, MZ Luthfi, ... Prosiding Seminar Nasional Pengabdian Kepada Masyarakat: Perguruan Tinggi … , 2022 2022
Effect of secretome of adipose stem cell (ASC) in photoaging skin WE Putri, A Endaryanto, D Tinduh, F Rantam, HB Notobroto, ... Bali Medical Journal 11 (3), 1212-1217 , 2022 2022 Citations: 3
MOST CITED SCHOLAR PUBLICATIONS
Mesenchymal Stem cell-Conditioned Medium (secretome) in skin aging: A systematic review WE Putri, CRSP Anang Endaryanto, Fedik Abdul Rantam International Journal of Pharmaceutical Research 13 (2) , 2021 2021 Citations: 7
Leprosy and Immune System: An Insight into the Innate Immune System PMC Winawati Eka Putri, S Budiamal Indian J Lepr, 391-403 , 2021 2021 Citations: 7
Skin barrier before and after topical Adipose Stem Cell-Conditioned Medium (ASC-CM) treatment in photoaging WE Putri, A Endaryanto, D Tinduh, F Rantam, HB Notobroto, ... Bali Medical Journal 10 (2), 688-691 , 2021 2021 Citations: 6
Depigmenting agent melanotoksik pada pengobatan melasma WE Putri, Y Kurniawati, T Djauhari Medical and Health Science Journal 2 (2), 23-31 , 2018 2018 Citations: 6
Growth factors of hypoxia Freeze-Dried Adipose Stem Cell-Conditioned Medium (FD ASC-CM) and Fresh Adipose Stem Cell Conditioned-Medium (FR ASC-CM): a comparative study WE Putri, A Endaryanto, D Tinduh, F Rantam, MD Alinda, ... Bali Medical Journal 10 (2), 683-687 , 2021 2021 Citations: 5
Pencegahan dan Penanganan Kasus Pedikulosis Kapitis di Lingkungan Pondok Pesantren I Farindra, WE Rusdi, WE Putri, VSP Saffanah, RY Nailuvar, SNW Putri, ... Jurnal Pemberdayaan Masyarakat Universitas Al Azhar Indonesia 6 (3), 190 , 2024 2024 Citations: 4
The relationship between fast food, sleep patterns, and facial hygiene with the severity of Acne vulgaris WE Putri, NF Hilda, FR Kusumah, AA Yulian, M Ulfa, MS Widyaswari, ... Biomolecular and Health Science Journal 7 (2), 88-95 , 2024 2024 Citations: 4
The effect of long exposure to uvb rays on histological features of wistar rats (Rattus norvegicus) in photoaging model N Hidayati, NA Hatikhah, WE Putri, MS Widyaswari, DS Muhammad, ... Bali Medical Journal 12 (3), 3078-3083 , 2023 2023 Citations: 4
Buku KIA Dalam Masa Pandemi Covid-19 R Amalia, I Mardiyanti, RAD Andriani, WE Putri, M Nasir Jurnal Paradigma (Pemberdayaan & Pengabdian Kepada Masyarakat) 3 (2), 39-44 , 2021 2021 Citations: 4
Profil Kelainan Kulit Pada Pasien Dermatitis Atopik Anak Dan Dewasa WE Putri, M Faizin, N Ainiyah Journal of Health Sciences 12 (1), 103-109 , 2019 2019 Citations: 4
Comparison of the histological features in the photoaged animal model WE Putri, MS Widyaswari, R Amalia, DS Muhammad, N Nisaussholihah, ... Bali Medical Journal 12 (3), 3064-3069 , 2023 2023 Citations: 3
Effect of secretome of adipose stem cell (ASC) in photoaging skin WE Putri, A Endaryanto, D Tinduh, F Rantam, HB Notobroto, ... Bali Medical Journal 11 (3), 1212-1217 , 2022 2022 Citations: 3
The Effect of Sunscreen Use on The Incidence of Melasma in Police Officers: Meta-Analysis and Systemic Review IN Putri, AP Putra, N Juniano, E Budi, WE Putri, R Mayangsari, ... International Islamic Medical Journal 6 (2), 12-24 , 2024 2024 Citations: 2
The Effect of Human Adipose Stem Cell-Conditioned Medium (hASC-CM) in Photoaged Skin WE Putri, MS Widyaswari, CRS Prakoeswa, DS Muhammad, ... Journal of Stem Cells & Regenerative Medicine 20 (2), 47 , 2024 2024 Citations: 2
Efektivitas Ekstrak Rimpang Jahe Merah (Zingiber Officinale Varietas Rubrum) Terhadap Pertumbuhan Malassezia Furfur Pada Pitiriasis Versikolor B Wibowo, MS Widyaswari, WE Putri PREPOTIF: JURNAL KESEHATAN MASYARAKAT Учредители: Universitas Pahlawan … , 2024 2024 Citations: 2
Penerapan Kamar Sehat untuk Mengurangi Kejadian Skabies di PP. Al Fitrah As Salafiyah Surabaya WE Putri, R Anggraini, EP Rahayu, Y Agnovianto Prosiding Seminar Nasional Pengabdian Kepada Masyarakat 3 (1), 273-277 , 2023 2023 Citations: 2
Effect of water clover (Marsilea crenata) leaf extract on estrogen receptors-β in skin aging WE Putri, M Ulfa Jurnal Ilmiah Kesehatan (The Journal of Health Sciences) 14 (1), 68-76 , 2021 2021 Citations: 2
Peningkatan Pengetahuan Penggunaan Tabir Surya untuk Mencegah Penuaan Ekstrinsik di RS Islam Jemursari Surabaya WE Putri, DS Muhammad, A Dinana, SE Ariefianto BERNAS: Jurnal Pengabdian Kepada Masyarakat 6 (1), 537-541 , 2025 2025 Citations: 1
The Effect Of Ethanolic Extract Of Katuk Leaf (Sauropus Androgynus) On Malondialdehyde (MDA) Levels In Ovalbumin-Induced Atopic Dermatitis Model Mice HSD Izzaty, WE Putri, S Syifak Asian Journal of Healthy and Science 4 (10), 353-364 , 2025 2025
The Pro-Apoptotic Effect of Diallyl Trisulfide (DATS) on MDA-MB-231 Breast Cancer Cells ESD Ailany, L Veterini, WE Putri, DW Kurniasari International Islamic Medical Journal 7 (1), 69-77 , 2025 2025
