Pharmacognosy and Phytochemistry, Phytopharmacology, Phytochemical analysis, Herbal supplements, Standardization
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Scopus Publications
Scopus Publications
Pharmacological activities of ruscogenin: A narrative review Preeti D. Verma, Sanskruti R. Rathod, Niyati J. Lad, Keval D. Prajapati, Arjunsinh A. Rathod, Mamta B. Shah Asian Pacific Journal of Tropical Biomedicine, 2026 Ruscogenin, a steroidal sapogenin, is primarily derived from the genus Ruscus. It demonstrates a wide range of pharmacological actions that hold substantial promise for therapeutic use. By conducting a comprehensive examination of electronic databases including PubMed, ScienceDirect, and SciFinder, we identified studies on ruscogenin to date. This review aims at emphasizing the findings related to the bioactivities of ruscogenin, particularly its protective activities in neurological and inflammatory disorders, hemorrhoids, and cancer. Ruscogenin has been demonstrated to possess anti-ulcer, anti-osteoporotic, and skin-lightening properties as a therapeutic agent. It has also been proven effective in managing metabolic disorders by reducing inflammation and improving lipid metabolism, particularly in conditions such as non-alcoholic steatohepatitis and diabetic nephropathy. Reports indicate that it can prevent acute lung injury by protecting pulmonary endothelial cells from apoptosis and modulating inflammatory cytokines. Additionally, ruscogenin promotes neurological recovery by mitigating oxidative stress. It also shows anti-cancer properties, leading to apoptosis and hindering metastasis, particularly in pancreatic and hepatocellular carcinoma. This review summarizes the potential medical applications of ruscogenin and underscores the need for further research on its mechanisms of action.
Development of validated HPTLC methods for determination of triterpenoids and flavan-3-ols in leaf and stembark of Manilkara hexandra Dubard Sapna Sharma, Karuna Modi, Milankumar Thakar, Gaurang Sindhav, Mamta B. Shah Natural Product Research, 2026 Manilkara hexandra Dubard is a highly reputed medicinal plant of Ayurveda. In the current study, High-performance Thin-layer chromatographic (HPTLC) techniques were established for the analysis of triterpenoids like lupeol (LU), betulinic acid (BA), epimeric flavan-3-ols (+)-catechin (CA), and (−)-epicatechin (ECA), in its leaf and stembark. HPTLC separation was performed using petroleum ether: ethyl acetate: toluene: formic acid (7:2:1:0.3, v/v/v/v) as a mobile phase to resolve LU and BA at Rf 0.51 and 0.34, respectively. Further, derivatization was done using a 1% anisaldehyde–sulphuric acid reagent. For CA and ECA, the mobile phase was di-isopropyl ether: ethyl acetate: formic acid (9:0.5:1, v/v/v), resulting in sharp bands at Rf 0.43 and 0.38. The result showed that the leaf contains high levels of BA and CA, while the stem bark is rich in LU and ECA. Both methods were simple, accurate, and reproducible and would potentially be useful for the standardisation of M. hexandra Dubard.
Quality control methods for fruit extracts of Kigelia africana using high performance thin layer chromatography Kunjan B. Bodiwala, Nancy Banker, Rahul Lalwani, Mamta B. Shah, Anuradha K. Gajjar Natural Product Research, 2025 Kigelia africana is a tree native to Africa but also found in eastern and southern parts of India with reported anti-bacterial, anti-inflammatory, and immunomodulatory activities. Verbascoside, caffeic acid and ferulic acid are important markers for the quality control of the plant. Two different HPTLC methods were developed and validated; method - 1 for estimation of verbascoside and caffeic acid while method - 2 for estimation of caffeic acid and ferulic acid. Developed methods were applied to the methanolic fruit extract to determine the quantities of markers. Both methods were found to be linear, specific, precise, accurate, sensitive and robust. Results indicated that both methods can be used for quantitative determination of verbascoside, caffeic acid and ferulic acid in fruit extract. The developed methods may be utilised as a part of the quality control and standardisation for the raw material and extracts of Kigelia africana and can also aid to chromatographic fingerprinting of the plant.
A Comprehensive Review on Biological and Chemical Diversity in Aesculus L. Genus (Sapindaceae) Mamta Shah, Rutvi Shah, Dhyey Salot, Dhvani Shah, Dhruvil Shah, Rajvi Shah, Preeti Verma Current Traditional Medicine, 2024 Aesculus L. is a genus of 12 to 19 woody species, representing one of the most acclaimed examples of inter-continental incoherence of the plants native to temperate regions of the Northern Hemisphere. It is traditionally valued in Ayurveda and the Chinese system of medicine for its various species that possess antitumor, cardioprotective, anti-inflammatory and neuroprotective activities. Current studies on this genus have revealed the presence of specific saponins with a multispectrum of pharmacological actions and therapeutic applications. A repertoire of literature related to the biological, taxonomical, phytochemical and ethnopharmacological aspects of Aesculus genus and its uses in different indigenous populations was explored. An inventory of all the species of this genus that were found to be useful as per ethnopharmacological literature was prepared. The ethnobotany, phytochemistry and pharmacology of the individual species are outlined in this review. The extensive information cited here has adduced the likely areas where the important species of this genus can illustrate significant therapeutic intervention in the management of chronic disorders including diabetes, hypercholesterolemia and cancer. The wide range of its pharmacological actions is attributed to the distinct phytochemicals occurring in the species, especially the triterpenoidal saponins like aescin. This review amplifies the recent studies on phytochemical and pharmacological aspects, which alludes that these species have noteworthy therapeutic potential.
Development of validated UHPLC-PDA with ESI-MS-MS method for concurrent estimation of magnoflorine, berbamine, columbamine, jatrorrhizine, palmatine and berberine in Berberis aristata Ishita A. Basera, Aboli Girme, Vijay P. Bhatt, Ganesh Saste, Sandeep Pawar, Lal Hingorani, Mamta B. Shah Acta Chromatographica, 2022 A validated UHPLC-PDA with an ESI-MS/MS method has been developed for simultaneous estimation of six bioactive alkaloids (magnoflorine, berbamine, columbamine, jatrorrhizine, palmatine and berberine) in the different extracts of the roots of Berberis aristata DC (Family:Berberdiaceae). It is an important medicinal herb native to Northern Himalaya and commonly known as ‘daruharidra’, ‘daruhaldi’, ‘Indian barberry’ or ‘tree turmeric’. An insight into the research literature uncovered reports on isoquinoline alkaloids like magnoflorine, berbamine, columbamine, jatrorrhizine, palmatine, and berberine as major bioactives in B. aristata roots, possessing different pharmacological and therapeutic effects. In the present study, these aforementioned alkaloids were separated on Phenomenex Luna®, 5 µm-C8 analytical column. The HPLC-MS analysis was performed at a flow rate of 0.90 mL min−1. Each alkaloid that is resolved was characterized by precursor ions and fragment ions with electrospray ionization (ESI) source in both positive and negative ionization using scan mode. The limit of detections (LODs) were 0.087, 0.727, 0.035, 0.124, 0.782 and 0.794 μg mL−1 for magnoflorine, berbamine, columbamine, jatrorrhizine, palmatine and berberine, respectively. The proposed UHPLC-PDA method was fully validated according to international (ICH) guidelines and was found to be selective, sensitive and highly accurate for the concomitant estimation of the aforementioned symbolic bio-markers of B. aristata roots.
Determination of dioecy in Hippophae salicifolia by evaluating gallic acid content through a validated HPTLC method Basera Ishita A., Verma Preeti D., Bhatt Vijay P., Shah Mamta B. Journal of Applied Pharmaceutical Science, 2022 Hippophae salicifolia D. Don, belonging to Elaeagnaceae family, exhibits dioecy and is extensively used for multipurpose due to its medicinal and nutraceutical potential. Although several research works have been carried out on the plant, no information is available regarding the impact of its dioecious nature on its phytochemicals and subsequent pharmacological properties. The present study is aimed at development of a high performance thin layer chromatography (HPTLC) method that is specific and reliable for the estimation of a marker compound gallic acid in both female and male counterparts of this dioecious plant, thus evaluating gender impact on chemical profile of the plant. For each sample, an ethyl acetate fraction of the hydrolyzed hydroalcoholic extract of H. salicifolia was prepared and applied on pre-coated plates of silica gel G 60F254. After developing the plate in the solvent system, toluene:ethyl acetate:formic acid (5:4:1, v/v/v), the identification and estimation was carried out by densitometric scanning at 270 nm. The presence of gallic acid was confirmed by UV overlay of the spot resolving in test tracks with that of reference standard. The results suggested quantitative variation of the marker compound in female and male plants, indicating a clear gender bias. The proposed validated HPTLC method is simple, yet specific and accurate and would contribute to the quality assessment of H. salicifolia.
High resolution-mass spectrometry (HR-MS) analysis of Bryonia laciniosa L. Indian Journal of Natural Products and Resources, 2021
Immunomodulatory and antioxidant activity of Curculigo orchioides Gaertn International Journal of Pharmtech Research, 2010
Development and validation of HPTLC method for determination of betulinic acid in helicteres isora root extract International Journal of Chemtech Research, 2010
An UV-visible spectrophotometric method for the estimation of plumbagin International Journal of Chemtech Research, 2010
