Mary Vinola Jenifer. S

@srmdentalcollege.ac.in

Senior lecturer, Department of conservative dentistry and endodontics
SRM Dental College Ramapuram

Mary Vinola Jenifer. S


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https://researchid.co/vinolaselvanathan

RESEARCH INTERESTS

dentistry, endodontics, aesthetics, root canal sealers
7

Scopus Publications

Scopus Publications

  • Evaluation of cytotoxicity and anti-inflammatory action of AH-Plus sealer with and without petasin, pachymic acid, curcumin & shilajit: an invitro-study
    Selvanathan M. J. Vinola, Sekar Mahalaxmi
    Scientific Reports, 2025
    AH Plus, an epoxy resin-based sealer extensively employed in endodontics, is renowned for its excellent dimensional stability and effective adhesion to dentin. Nevertheless, extensive clinical research has documented its propensity to induce inflammation, especially when freshly mixed. Phytochemicals such as petasin, pachymic acid, curcumin, and shilajit are known for their anti-inflammatory and analgesic properties. This study aimed to investigate the influence of these phytochemicals on cytotoxicity and anti-inflammatory potential of AH Plus. Experimental groups included AH Plus-resin sealer (AHR) with and without 10% petasin (AHP), 0.75% pachymic acid (AHA), 0.5% curcumin (AHC) and 6% shilajit (AHS) at ratios of 5:1 and 10:1 respectively. Stem cells of apical papilla (SCAP) were exposed to the experimental sealers and assessed for cytotoxicity (MTT assay) and cytokine expression levels of IL-6, IL-8 and TNF-α (ELISA) at different time intervals (0 h, 24 h and 48 h). Experiments were performed in triplicate and analyzed using one-way ANOVA (p < 0.05). All experimental groups demonstrated varying levels of decreased cytotoxicity and cytokine expression compared to the control group; specifically in 5:1 ratio. Thus, it can be concluded that addition of petasin, pachymic acid, curcumin, and shilajit with AH Plus sealer in 5:1 ratio resulted in decreased cytotoxicity and enhanced anti-inflammatory properties.
  • Characterisation and evaluation of physical properties of AH-Plus sealer with and without the incorporation of petasin, pachymic acid, curcumin and shilajit-an invitro study
    Selvanathan MJ Vinola, Sekar Mahalaxmi
    BMC Oral Health, 2024
    Background AH Plus, an epoxy resin-based sealer, is widely used in endodontic practice, owing to its good physical properties that confers longstanding dimensional stability and good adhesion to dentin. Nevertheless, its propensity to trigger inflammation, especially in its freshly mixed state, has been extensively documented. Phytochemicals such as Petasin, Pachymic acid, Curcumin, and Shilajit are known for their anti-inflammatory and analgesic effects. This study aimed to analyze and determine the effect of these natural products on the physical properties of AH Plus sealer when incorporated with the sealer. Methods AH Plus (AHR) sealer was mixed with 10% petasin, 0.75% pachymic, 0.5% and 6%shilajit to obtain AHP, AHA, AHC and AHS in the ratio of 10:1 and 5:1 respectively. Five samples of each material were assessed for setting time, solubility, flow, and dimensional stability in accordance with the ISO 6876:2012 standardization. Sealers were characterized through scanning electron microscopy (SEM), X-ray energy dispersive spectroscopy, X-ray diffraction (XRD), and Fourier transform infrared (FTIR) spectroscopy. Statistical evaluation involved the Kolmogorov-Smirnov and Shapiro-Wilks tests for normality and the one-way ANOVA test for analysis. Results In this investigation, the characterisation analysis revealed a relatively similar microstructure in all the experimental root canal sealers. All experimental groups, excluding the control group, exhibited an increase in flow ranging from 11.9 to 31.4% at a 10:1 ratio. Similarly, for the 5:1 ratio, the increase ranged from 12.02 to 31.83%. In terms of dimensional stability, all groups at the 10:1 ratio showed a decrease compared to the control group. The addition of natural agents to AHR in 10:1 ratio led to a reduction in setting time by 8.9–31.6%, and at a 5:1 ratio, the reduction ranged from 8.1 to 31.5%. However, regarding solubility, the addition of natural agents did not induce any significant alterations. Conclusion Based on the results of this study, it can be concluded that all tested root canal sealers exhibited properties that met the acceptable criteria outlined in the ISO 6876:2012 standardization.
  • The evaluation of cytotoxicity and cytokine IL-6 production of root canal sealers with and without the incorporation of simvastatin: an invitro study
    Apoorva Sharma, Kavitha Sanjeev, Vinola M. J. Selvanathan, Mahalaxmi Sekar, Nikhil Harikrishnan
    BMC Oral Health, 2022
    Background Freshly mixed root canal sealers when proximate the periapical tissues, trigger varying degrees of cytotoxicity/inflammatory reactions. Simvastatin, a class of the drug statin, is a widely used cholesterol-lowering agent with additional anti-inflammatory activities. This study assessed the effects of simvastatin on cytotoxicity and the release of IL-6 (Interleukin-6) production when incorporated in zinc oxide eugenol and methacrylate resin-based sealers. Methods Experimental groups consisted of conventional zinc oxide eugenol and methacrylate based-EndoREZ sealers (ZE &amp; ER respectively) and 0.5 mg/mL simvastatin incorporated sealers (ZES &amp; ERS). L929 mouse fibroblast cells were exposed to freshly mixed experimental sealers and evaluated for cytotoxicity (MTT assay) and inflammation levels (inflammatory marker IL-6 for ELISA) at various time intervals (0h, 24h and 7th day). The values were compared to the cell control (CC; L929 cells alone) and solvent control (SC; L929 cells + DMSO) groups. All the experiments were conducted in triplicates and subjected to statistical analysis using IBM SPSS Statistics software. Non parametric tests were conducted using Kruskal-Wallis and Friedman tests for inter-group and intra-group comparisons respectively. Pairwise comparison was conducted by post hoc Dunn test followed by Bonferroni correction. P values &lt; 0.05 were considered statistically significant. Results All the experimental groups (ZE, ER, ZES, ERS) exhibited varying degree of cytotoxicity and IL-6 expression compared to the control groups CC and SC. The cell viability for ZE and ER decreased on day 7 as compared to 24 h. ZES and ERS had higher viable cells (75.93% &amp; 79.90%) compared to ZE and ER (54.39% &amp; 57.84%) at all time periods. Increased expression of IL-6 was observed in ZE &amp; ER (25.49 pg/mL &amp; 23.14 pg/mL) when compared to simvastatin incorporated ZE &amp; ER (ZES-12.70 pg/mL &amp; ERS-14.68 pg/mL) at all time periods. Highest level of cytotoxicity and inflammation was observed in ZE compared to all the other groups on day 7. Conclusions Addition of 0.5 mg/mL of simvastatin to the sealers (ZES and ERS) decreased the cytotoxicity in the freshly mixed state and reduces their inflammatory effect.
  • Anti-inflammatory efficacy of petasin-incorporated zinc oxide eugenol sealer - An in vivo zebrafish study
    SelvanathanM J. Vinola, Kittappa Karthikeyan, Apoorva Sharma, Sai Sudheshna, Mahalaxmi Sekar
    Journal of Conservative Dentistry, 2021
    Background: The eugenol from zinc oxide eugenol (ZnOE) sealer tends to diffuse to the periapical region resulting in inflammation. Several modifications of ZnOE sealer have been formulated to minimize the inflammatory potential of the ZnOE sealer. Petasites hybridus contains petasin which possesses anti-inflammatory property used in treatment of migraine and allergic rhinitis. Aim: The aim of the study was to evaluate the anti-inflammatory property of petasin-incorporated ZnOE sealer on zebrafish. Materials and Methods: The study has been reviewed and approved by the Institutional Review Board (SRMU/MandHS/SRMDC/2018/S/025) and by the in-house Institutional Animal Ethics Committee (IAEC) of Pentagrit Research Lab and conducted with compliance to ICH harmonization and principles for animal housing and handling (IAEC Study No: 215/Go06/IAEC). The samples were implanted in the caudal portion of the zebrafish. The samples (n = 50) were divided into five groups (n = 10) – Group 1: incision only (negative control), Group 2: zinc oxide (ZnO) (positive control), Group 3: ZnO + eugenol and petasin in ratio of 10:1, Group 4: ZnO + eugenol and petasin in ratio of 5:1, and Group 5: ZnO + eugenol and petasin in ratio of 1:1. The experimental groups were further subdivided into two subgroups based on time intervals at 24 h and 48 h. The tissue samples were assessed using smear pathology test, and the percentage of inflammation was evaluated. The data were statistically analyzed using SPSS software version 22.0 with a significance level fixed as 5% (α = 0.05). Results: The presence of inflammatory cells and congestion of arterioles were taken as the criteria to assess inflammatory action. It was maximum in ZnOE sealer followed by ZnOE sealer modified with the addition of petasin to eugenol in the ratio of 10:1, 5:1, and 1:1, respectively. Conclusion: The addition of petasin extract to ZnOE reduces the inflammation potential of ZnOE sealers. ZnOE sealer containing eugenol and petasin in the ratio of 1:1 showed a maximal reduction in inflammation.
  • COVID‑19 and Its Impact on Dental Health‑care Professionals
    SelvanathanM J. Vinola, Mahalaxmi Sekar
    Journal of Interdisciplinary Dentistry, 2021
    The COVID-19 pandemic has taken a significant toll on several fields, with dentistry being one of the most affected. This editorial outlines the heavier impacts of COVID-19 outbreak on dental health-care professionals. Further, this editorial emphasizes on adoption of strict standard operating protocols for health-care professionals to combat the COVID-19 disease transmission. Dentistry is facing its time of strenuous survival, with the outburst of the COVID pandemic. After the publications of dental academics in Wuhan, China, the response of the dental professionals and its impact on them became an eminent and petrifying entity in the field of dentistry.[1,2,3,4] This report, outlined the immediate impact that the COVID-19 outbreak currently had on dental health-care professionals and also on the massive impacts it might have on the profession of dentistry. Dental surgeons are at extreme risk of contracting and transmitting the coronavirus, alongside paramedics, nurses, and other health-care workers. According to the Occupational Safety and Health Administration (OSHA), dentists work in close proximity to the patient's oral cavity and are therefore placed in very high exposure risk category.[5,6] Since it is a known fact that dental procedures commonly generate blood and saliva droplets resulting in the spread of the virus, the American Dental Association (ADA) suggested dental practitioners globally to limit their interventions to emergency treatments.[7] Likewise, the department of health and dental organizations of affected countries also appealed the dentists to postpone nonurgent dental procedures.[8] The dentists were advised to only treat pain and sepsis, using the strictest infection and transmission control protocol. Irrespective of aerosol production, other emergencies such as bleeding following dental procedures, oral cancers, and fractures were advised to be treated.[9,10] During the first wave of COVID-19, dental clinics across the country were nonfunctional for over 5 months which led to disrupted dental services to the patients and tremendous depletion in the economy of dentists. In the first phase of the pandemic in particular, the aseptic protocols and recommended protective materials were not accessible, and blueprints were missing to provide clear guidance for dentists. In addition, the lack of personal protective equipment (PPE) complicated the working environment for dentists during the first wave. In the USA, a survey involving 20,000 US dentists reported that nine out of ten dental practices had less than a quarter of their typical patient volume, with 82% of dental practices having had less than a quarter of typical income and revenue.[11] Despite meticulous and stern global containment and curfew efforts, the incidence of COVID-19 still prevails and has led to the outbreak of the second wave. In India, the second wave of COVID is destructive in nature being a threat to life and economy, with devastating cases causing drastic increase beyond measure. However, even at these hard times, most of the dental practitioners were well prepared ahead and have successfully worked through the pandemic without getting infected, by following proper protocols given by the WHO, OSHA, and ADA. Even though it is a chaotic situation for dentistry that shall continue till the development and availability of a long-lasting, permanent, and efficient vaccination, it gives positivity and hope to find our colleagues and friends triumph in their dental practice over all the obstacles caused by the pandemic. It is at these hard times we as dentists should labor in finding better solutions to keep our much-needed profession thrive among the frontline of health care. As doctors, it is our responsibility to take care of our patients in case of need during this pandemic. We should care for our patients through online consultation, with reduced contact, restricting the generation of aerosols, by using PPE and by following all the aseptic protocols. In this difficult situation, it is our duty and morale to support the members of our fraternity including our students and staffs. We should give prime attention to our own mental and physical well-being as well as helping each other to cope up the situation. Always remember, the good news of the coming back to normal of the routine dental care in many countries around the world is a ray of hope. Let's not lose hope “Change is the only constant.” Let's embrace it with the strong belief that this too will pass.
  • Comparative Evaluation of Calendula officinalis and 2% Chlorhexidine against Enterococcus faecalis and Candida albicans
    SelvanathanM. J. Vinola, Mahalaxmi Sekar, SenthilKumar Renganathan, Selvanathan Dhiraviam
    Journal of Interdisciplinary Dentistry, 2021
    Background: Endodontic infections require effective removal of microorganisms from the root canal system for long-term prognosis. Chlorhexidine (CHX) is one of the most effective irrigants, but it's few drawbacks warrant search for newer alternatives. Aim: The aim of this study is to evaluate the antimicrobial efficacy of Calendula officinalis (CO) and 2% CHX against Enterococcus faecalis (E. faecalis) and Candida albicans (C. albicans). Materials and Methods: The antimicrobial activity of CO extract and 2% CHX against E. faecalis and C. albicans was checked by the agar diffusion method and the zone of inhibition was statistically analyzed. Results: CHX has significantly more antimicrobial activity than CO against E. faecalis and C. albicans. However, CO also possesses reasonable antimicrobial activity against E. faecalis and antifungal activity against C. albicans. Conclusion: Under the limitations of this study, it can be concluded that CO has antimicrobial and antifungal activity against E. faecalis and C. albicans; however, CHX is more potent.
  • A novel petasin-modified zinc oxide eugenol sealer
    SelvanathanM J. Vinola, Kittappa Karthikeyan, Sekar Mahalaxmi
    Journal of Conservative Dentistry, 2019
    Objective: Zinc oxide eugenol (ZOE) is one of the most commonly used root canal sealer. However, it has few drawbacks such as cytotoxicity, solubility, and irritation to periapical tissues. The scope of this study was to investigate the setting time, solubility, cytotoxic effects, and anti-inflammatory action of ZOE sealer with the modification of its liquid component by the addition of petasin extract in the ratios 1:1, 5:1, and 10:1. Materials and Methods: Setting time was evaluated using the Vicat's apparatus. For testing solubility, the American Dental Association's specification #8 was adopted with certain modifications. Protein denaturation assay and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay with L929 mouse fibroblast cell lines were used to evaluate the anti-inflammatory property and cytotoxicity, respectively. Results: ZOE sealer with petasin extract in the ratio of 5:1 showed the least initial and the final setting times. There was no statistically significant difference in the amount of solubility for all the groups at the various time intervals. The cytotoxicity of the control group was significantly greater than all the experimental groups, whereas the anti-inflammatory effect of the former was statistically lower. Conclusions: The combination of ZOE with petasin extract in the ratio of 5:1 showed lower setting time, cytotoxicity, and better anti-inflammatory property.