Dr. Prasad Deepak Dandekar

@mitbio.edu.in

School of Bioengineering Sciences and Research, MITADT University
Assistant Professor

Dr. Prasad Deepak Dandekar


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https://researchid.co/prasaddand

RESEARCH INTERESTS

Antidiabetics, Antiviral Drugs, Phytochemistry
3

Scopus Publications

64

Scholar Citations

3

Scholar h-index

2

Scholar i10-index

Scopus Publications

  • Optimization of Fermentation Conditions Using Design of Experiment (DoE) to Enhance the Production of Antigenic Capsular Polysaccharides From Streptococcus pneumoniae Serotype 18C for Pneumococcal Vaccine Development
    Amol Dattatraya Mahajan, Pramod Maruti Jadhav, Avinash Rohidas Memane, Gautam Kumar Jha, Swapan Kumar Jana, Prasad Deepak Dandekar, Pranav Deepak Pathak
    Biotechnology and Applied Biochemistry, 2026
    Pneumococcal pneumonia, caused by Streptococcus pneumoniae , has emerged as a global health threat, especially in children below 5 years of age and elderly population. S. pneumoniae remains a major global health threat which necessitates scalable and high‐yield vaccine production. This study presents an innovative fermentation platform for S. pneumoniae (serotype 18C) by optimizing an already refined media composition using data‐driven Design of Experiment (DoE) approach. Unlike conventional applications, Plackett–Burman design (PBD) was strategically employed beyond screening, identifying statistically significant parameters and the interaction between key factors of dextrose, MgSO 4 , pH, and aeration. Results reveal that pH level of 7.1 significantly enhances the antigenic capsular polysaccharide yield. Further, ANOVA analysis confirmed a strong synergistic effect between dextrose and MgSO 4 . In addition, the present study also finds that fermentation at lower level of aeration (0.2 vvm) favors the bacterial growth aligning with S. pneumoniae ’s facultative anaerobic or microaerophilic nature. The downstream processing integrating centrifugation and diafiltration (100 kDa) improves the product recovery and purity reinforcing the process efficiency. The unconventional application of PBD combined with systematic optimization of refined process sets a new benchmark for pneumococcal fermentation strategies. The findings demonstrate that precise modulation of fermentation conditions can enhance the vaccine antigen yield addressing the scalability challenges in industrial production. Future work may focus on extending this DoE‐based platform to other serotypes of S. pneumoniae and refining fermentation kinetics for next generation pneumococcal vaccine. This study not only challenges the traditional experimental set up, but paves the way for adaptive, data‐driven bioprocess innovations in vaccine manufacturing.
  • Insights into the Inhibition Mechanism of Human Pancreatic α-Amylase, a Type 2 Diabetes Target, by Dehydrodieugenol B Isolated from Ocimum tenuiflorum
    Prasad D. Dandekar, Amol S. Kotmale, Shrawan R. Chavan, Pranita P. Kadlag, Sangeeta V. Sawant, Dilip D. Dhavale, Ameeta RaviKumar
    ACS Omega, 2021
    Use of human pancreatic α-amylase (HPA) inhibitors is one of the effective antidiabetic strategies to lower postprandial hyperglycemia via reduction in the dietary starch hydrolysis rate. Many natural products from plants are being studied for their HPA inhibitory activity. The present study describes isolation of dehydrodieugenol B (DDEB) from Ocimum tenuiflorum leaves using sequential solvent extraction, structure determination by one-dimensional (1D) and two-dimensional (2D) NMR analyses, and characterization as an HPA inhibitor using kinetics, binding thermodynamics, and molecular docking. DDEB uncompetitively inhibited HPA with an IC50 value of 29.6 μM for starch and apparent Ki′ of 2.49 and Ki of 47.6 μM for starch and maltopentaose as substrates, respectively. The circular dichroism (CD) study indicated structural changes in HPA on inhibitor binding. Isothermal titration calorimetry (ITC) revealed thermodynamically favorable binding (ΔG of −7.79 kcal mol–1) with a dissociation constant (Kd) of 1.97 μM and calculated association constant (Ka) of 0.507 μM. Molecular docking showed stable HPA–inhibitor binding involving H-bonds and Pi-alkyl, alkyl–alkyl, and van der Waals (vDW) interactions. The computational docking results support the noncompetitive nature of DDEB binding. The present study could be helpful for exploration of the molecule as a potential antidiabetic drug candidate to control postprandial hyperglycemia.
  • Structure–activity relationships of pancreatic α-amylase and α-glucosidase as antidiabetic targets
    Prasad Dandekar, Sudha Ramkumar, Ameeta RaviKumar
    Studies in Natural Products Chemistry, 2021

RECENT SCHOLAR PUBLICATIONS

  • Optimization of Fermentation Conditions Using Design of Experiment (DoE) to Enhance the Production of Antigenic Capsular Polysaccharides From Streptococcus pneumoniae Serotype …
    AD Mahajan, PM Jadhav, AR Memane, GK Jha, SK Jana, PD Dandekar, ...
    Biotechnology and Applied Biochemistry , 2026
    2026
  • Journey with jaundice: a narrative review on the global health perspective of hepatitis A virus and its impact on the modern world
    PD Dandekar
    Emerging Human Viral Diseases, Volume II: Encephalitic, Gastroenteric, and … , 2024
    2024
    Citations: 3
  • GLYCOSIDASE INHIBITOR, PROCESS FOR ITS PREPARATION, AND APPLICATIONS THEREOF
    AR Prasad Deepak Dandekar
    IN Patent App. 201921036657 A , 2021
    2021
  • Insights into the Inhibition Mechanism of Human Pancreatic α-Amylase, a Type 2 Diabetes Target, by Dehydrodieugenol B Isolated from Ocimum tenuiflorum
    PD Dandekar, AS Kotmale, SR Chavan, PP Kadlag, SV Sawant, ...
    ACS omega 6 (3), 1780-1786 , 2021
    2021
    Citations: 48
  • Structure–activity relationships of pancreatic α-amylase and α-glucosidase as antidiabetic targets
    P Dandekar, S Ramkumar, A RaviKumar
    Studies in Natural Products Chemistry 70, 381-410 , 2021
    2021
    Citations: 13

MOST CITED SCHOLAR PUBLICATIONS

  • Insights into the Inhibition Mechanism of Human Pancreatic α-Amylase, a Type 2 Diabetes Target, by Dehydrodieugenol B Isolated from Ocimum tenuiflorum
    PD Dandekar, AS Kotmale, SR Chavan, PP Kadlag, SV Sawant, ...
    ACS omega 6 (3), 1780-1786 , 2021
    2021
    Citations: 48
  • Structure–activity relationships of pancreatic α-amylase and α-glucosidase as antidiabetic targets
    P Dandekar, S Ramkumar, A RaviKumar
    Studies in Natural Products Chemistry 70, 381-410 , 2021
    2021
    Citations: 13
  • Journey with jaundice: a narrative review on the global health perspective of hepatitis A virus and its impact on the modern world
    PD Dandekar
    Emerging Human Viral Diseases, Volume II: Encephalitic, Gastroenteric, and … , 2024
    2024
    Citations: 3
  • Optimization of Fermentation Conditions Using Design of Experiment (DoE) to Enhance the Production of Antigenic Capsular Polysaccharides From Streptococcus pneumoniae Serotype …
    AD Mahajan, PM Jadhav, AR Memane, GK Jha, SK Jana, PD Dandekar, ...
    Biotechnology and Applied Biochemistry , 2026
    2026
  • GLYCOSIDASE INHIBITOR, PROCESS FOR ITS PREPARATION, AND APPLICATIONS THEREOF
    AR Prasad Deepak Dandekar
    IN Patent App. 201921036657 A , 2021
    2021