Response Surface Methodology for Optimization of Mucilage Extraction from Dioscorea alata Tuber Dewi Fortuna, , Emmy Darmawati, Titi Candra Sunarti, Sutrisno Suro Mardjan, Siti Mariana Widayanti, Nancy Dewi Yuliana, Nanik Purwanti, , , , , , and Natural and Life Sciences Communications, 2024 Dioscorea alata mucilage (DAM) is a water-soluble polysaccharide and is composed of glucomannan. In this research, mucilages from D. alata were extracted using Response surface methodology (RSM) with a Central Composite Design (CCD) followed by distance-based optimality design, with three independent variables applied, i.e., temperature (4 – 60°C), time (0 – 30 h), and centrifugal speed (1.386 – 22.615×g). The maximum DAM yield was 5.89% ± 0.50%, which was obtained with an extraction temperature of 36.2°C for 13.3 h extraction time and centrifugation at 12,322×g. The regression model from RSM – CCD predicted that the maximum DAM yield was 5.47%, with R2 of 99.59% and adjusted R2 of 97.72%, which shows a significant correlation between the experimental and predicted values. The DAM is a complex material that mainly contains polysaccharides, i.e., β-pyranose forms of glucose and mannose, with low protein content. Water and oil holding capacity of the DAM powder were 8.43 g/g and 5.08 g/g, respectively, which considered as the highest compared to other similar studies. Keywords: Dioscorea alata mucilage, Extraction, Optimization, Response surface methodology, Physicochemical properties
Extraction and characteristic of Dioscorea alata mucilage D Fortuna, S S Mardjan, T C Sunarti, E Darmawati, S M Widayati, N Purwanti Iop Conference Series Earth and Environmental Science, 2020 Dioscorea alata (DA) mucilage is classified as water-soluble polysaccharides, which has potential as a source of hydrocolloid. This research was conducted to investigate effects of salt types on water to tuber ratio to produce the most optimal mucilage yield. This research was conducted using Completely Randomized Factorial Design 3 x 5. The DA mucilage was extracted using two factors, i.e., types of salt (without salt, sodium chloride, and calcium chloride) and water to tuber ratios (2:1, 4:1, 6:1, 8:1, and 10:1). The results showed that the types of salt significantly affect (P<0.05) on starch content, but did not have a significant effect on mucilage yield and water content of the mucilage. The water to tuber ratios significantly affected (p<0.05) the mucilage yield and starch content of the mucilage, however it did not affect the water content. Addition of calcium chloride when water to tuber ratio was 4:1 was recommended to produce the optimal mucilage yield (1.58%) with relatively low starch content (7.86%).
The study of physical properties of dioscorea alata's starch from jambi province - Ulyarti, - Lavlinesia, Dewi Fortuna, - Surhaini International Journal on Advanced Science Engineering and Information Technology, 2016 Botanical origin of a plant is known to affect the physical characteristic of its tuber. This research was conducted to investigate the physical characteristic of starch obtained from Dioscorea sp tubers grown in Jambi Province. There were two cultivars used in this experiment: yellow and purple tuber. The tubers were extracted using three methods: control, soaking and steam blanching. Soaking was performed by soaking the small cut tubers in hot water at 60 o C for 15 minutes while steam blanching was performed by steaming them at 100 o C for 5 minutes. The starches were examined for their colour parameter, swelling power (SP) and solubility. SP and solubility of starch were observed at several temperatures from 65 to 95 o C. Type of cultivar and the extraction method significantly affect L*, a* and b* value of the starch. SP at 65 o C, 70 o C and 75 o C were found to be affected by both the type of cultivar (p<0.01)) and the extraction method (p<0.01). Steam blanching was found to increase SP of purple cultivars while soaking was found to increase SP of yellow cultivar. On the other hand, the solubility of starch was not affected by either the type of cultivar or the extraction method
Degradation of acid cyanide poison in rubber seed (Hevea brasiliensis) after treatment with rice husk ash Dewi Fortuna, Abdul Rahimsyah, Yuniwati Puspitasri International Journal on Advanced Science Engineering and Information Technology, 2015 Rubber seed (Hevea brasiliensis) contains protein (17.41 %) and non-essential amino acid cysteine (0.78 %) and acid cyanide poison (186.00 mg/kg). The purpose of this research was to determine the effect of rice husk ash on degradation of acid cyanide in rubber seed. This research used Completely Randomized Design (CRD) using treatment of rice husk ash concentration with 5 levels of treatments (45; 60; 75; 90; 105 %) and 4 replications. The result showed nonsignificant differences (p>0.05) in degradation of acid cyanide level during aging and significant differences (p<0.05) during soaking. Rubber seed treated with 90% of rice husk ash during aging period contained cyanide 47.25 ppm and rubber seed treated with 60% of rice husk ash during soaking period contained cyanide 40.37 ppm.
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