HARISCHANDRA NAIK R

I have subject expertise in Pesticide Toxicology, Pesticide Residue, Food Contaminants and Food Quality Analysis; and Insecticide Resistance and Management. I have ten years of experience in teaching, research, and extension activities at UAS, Raichur. I have been involved in developmental activities such as the establishment of Pesticide Residue and Food Quality Analysis Laboratory and obtaining the NABL accreditation. Involved in the establishment of a pesticide formulation testing laboratory.

EDUCATION

Ph.D in Agricultural Entomology-2012

RESEARCH INTERESTS

Pesticide Toxicology
Pesticide Residue Analysis
Insecticide Resistance and Management

Scopus Publications

Method Development and Validation for Determination of Indoxacarb Using LC-ESI-MS/MS and Its Dissipation Kinetics in Pigeonpea
Harischandra R Naik, M. S Pallavi, Rahul Chawan, M Bheemanna, Anand Naik, M Paramasivam
Food Analytical Methods, 2020
A simple, sensitive and reproducible analytical method for quantitative determination of indoxacarb residues in pigeonpea green pod, pigeonpea dry grain and soil was developed and validated using LC-MS/MS. The developed methodology was linear having correlation coefficients (R 2 ) value of 0.999. The LOD and LOQ were 0.0015 and 0.005 μg g −1 , respectively. QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) technique involved for extraction of indoxacarb residues with 1% ethyl acetate in acetonitrile provided acceptable recoveries in the range of 83.80–99.01% at 0.005, 0.025 and 0.050 μg g −1 spiking level with precision RSD of less than 1.20 %. Initial deposit on green pods following two application (15 days interval between application) of indoxacarb 14.5% SC at 60 and 120 g a.i.ha −1 was 0.583 and 1.342 μg g −1 and dissipated to 0.005 and 0.006 μg g −1 on 7 and 10 days after application, respectively. Half-life and safe waiting period (SWP) were ranged from 1.13 to 1.23 days and 3.23 to 5.03 days for the dose 60 and 120 g a.i.ha −1 in pigeonpea green pods, respectively. Hazard index (HI) value was more than 1 in green pods drawn on 5th and 7th day after application in recommended (60 g a.i.ha −1 ) and double the recommended dose (120 g a.i.ha −1 ), respectively.
Assessment of crop loss due to leafhopper, (Empoasca kerri pruthi) in pigeonpea
Rachappa V., Shivayogiyappa ., Harischandra N., Suhas Yelshetty
Legume Research, 2018
An experiment was conducted at Agriculture Research Station, Kalaburagi, Karnataka, India during kharif, 2015-16 to understand the effect of differential population of leafhoppers, on growth and yield parameters of pigeonpea under field conditions with seven level of population load. Maximum of 26.16 hoppers/3 leaves were found at 12 pairs release plot which was at par with 10 pairs (22.21) and 8 pairs (24.96) released which were statistically significant. The average hopper count @ 4 and 6 pairs released plots were 13.14 and 16.15 per 3 leaves respectively and were at par among themselves and significantly higher than 2 pairs release and lower than 8 pairs release plots. This was a clear indication that the differential population of leafhopper had impact in reduction of growth and yield parameters with. Reduction in yield to the tune of 3.12, 5.29, 10.05, 17.99, 24.63 and 31.91 % at 2, 4, 6, 8, 10 and 12 pairs released plots, respectively. From the present investigation it was clear that the 5.59 to 5.77 leafhoppers aggregated in top leaf at 90 to 120 days aged crop of medium duration variety will cause 10 per cent reduction in yield.
Phosphine resistance in India is characterised by a dihydrolipoamide dehydrogenase variant that is otherwise unobserved in eukaryotes
R Kaur, M Subbarayalu, R Jagadeesan, G J Daglish, M K Nayak, H R Naik, S Ramasamy, C Subramanian, P R Ebert, D I Schlipalius
Heredity, 2015
Phosphine (PH3) fumigation is the primary method worldwide for controlling insect pests of stored commodities. Over-reliance on phosphine, however, has led to the emergence of strong resistance. Detailed genetic studies previously identified two loci, rph1 and rph2, that interact synergistically to create a strong resistance phenotype. We compared the genetics of phosphine resistance in strains of Rhyzopertha dominica and Tribolium castaneum from India and Australia, countries having similar pest species but widely differing in pest management practices. Sequencing analysis of the rph2 locus, dihydrolipoamide dehydrogenase (dld), identified two structurally equivalent variants, Proline49>Serine (P49S) in one R. dominica strain and P45S in three strains of T. castaneum from India. These variants of the DLD protein likely affect FAD cofactor interaction with the enzyme. A survey of insects from storage facilities across southern India revealed that the P45/49S variant is distributed throughout the region at very high frequencies, in up to 94% of R. dominica and 97% of T. castaneum in the state of Tamil Nadu. The abundance of the P45/49S variant in insect populations contrasted sharply with the evolutionary record in which the variant was absent from eukaryotic DLD sequences. This suggests that the variant is unlikely to provide a strong selective advantage in the absence of phosphine fumigation.
Determination of imidacloprid residues in mulberry leaves by quechers and liquid chromatography with diode array detection
M. Paramasivam, S. Chandrasekaran, R. Harischandra Naik, P. Karthik, P. Thangachamy, C. A. Mahalingam
Journal of Liquid Chromatography and Related Technologies, 2014
A simple and sensitive QuEChERS method for the determination of imidacloprid residues in mulberry leaf was developed using high performance liquid chromatography (HPLC). This method was then evaluated for the residual level and dissipation rate of imidacloprid in mulberry leaves. A maximum initial deposits of imidacloprid on mulberry leaves was found to be 3.047 µg g−1 and 6.490 µg g−1 following two application rates of imidacloprid 17.8 SL at 0.6 and 1.2 mL L−1. The calculated half-life of imidacloprid in mulberry leaves was at recommended dose 3.81 d with a dissipation rate of 76% over 7 d; whereas, in double, the dose was 4.93 days with a dissipation rate of 84% over 10 d.

RECENT SCHOLAR PUBLICATIONS

Study on Biology of Microcephalothrips abdominalis Crawford (Thysanoptera: Thripidae) on Marigold under Laboratory Conditions
LT Nikita, K Thulasiram, G Narabenchi, RH Naik, B Doddabasappa, ...
UTTAR PRADESH JOURNAL OF ZOOLOGY 47 (2), 45-51 , 2026
2026
Field Screening of Chilli Genotypes Against the Incidence of South East Asian Thrips, Thrips parvispinus (Karny)
S a Nyamagoud, G Narabenchi, RH Naik, M Sood, KD Gaddennavar
UTTAR PRADESH JOURNAL OF ZOOLOGY 47 (2), 12-19 , 2026
2026
Insecticide induced resurgence of Scirtothrips dorsalis Hood: Role of biochemical changes in chilli and natural enemies suppression
T Hemadri, M Bheemanna, DS Reddy, A Hosamani, RH Naik, SN Rao, ...
Plant Science Today 12, 9034 , 2025
2025
Simultaneous determination of persistent pesticide residues in pomegranate matrices using GC–MS/MS and its dietary risk assessment
RH Naik, K Pavankumar, MS Pallavi, A Shwetha, M Saraswati, ...
Microchemical Journal, 115193 , 2025
2025
Citations: 2
Evaluation and Identification of Novel Insecticidal Toxin Genes From Bacillus Thuringiensis (Berliner) Isolates
M Naik, B Kalmath, S Rao, S Dastager, A Hosamani, B Kadannavar, ...
Indian Journal of Entomology, 323-330 , 2025
2025
Determination of cyantraniliprole in green and red chilli fruits and Indian soil using LC-MS/MS and its dietary risk assessment
G Poornima, RH Naik, MS Pallavi, M Saraswati, P Ratnamma, A Shwetha, ...
Journal of Food Composition and Analysis 141, 107294 , 2025
2025
Citations: 5
Impact of weather parameters on the seasonal incidence of Helopeltis antonii (Miridae: Hemiptera) on neem
BRC Police-Patil, M Bheemanna, A Prabhuraj, MS Murthy, ...
Acta Scientiarum. Biological Sciences 47, e74081 , 2025
2025
Impact of weather parameters on the seasonal incidence of Helopeltis antonii (Miridae: Hemiptera) on neem.
PPBR Chaitanya, M Bheemanna, A Prabhuraj, MS Murthy, ...
Acta Scientiarum: Biological Sciences 47 (1) , 2025
2025
LC-MS/MS and GC–MS/MS quantification of pesticide residues in paddy grains and processed rice and dietary risk assessment
AP M. Saraswati, R. Harischandra Naik, M.S. Pallavi, M. Bheemanna, M ...
Microchemical Journal 113391, 112516 , 2025
2025
Citations: 8
Validation and multiresidue analysis of pesticides in seed, oil and cake of groundnut using triple quadrupole LC-MS/MS along with uncertainty analysis and risk assessment
H Naik R
Microchemical Journal 209, 112516 , 2025
2025
Citations: 7
Assessment of neonicotinoids toxicity and their risk to honeybee under caged and open field ecosystem
H Naseema, P Ratnamma, K Babu, M Bheemanna, B PR
2024
Citations: 1
Status of Grain Discoloration Disease in Paddy Growing Ecosystems of Karnataka, India
H Sharanabasav, SK Jayalakshmi, S Gururaj, NR Harischandra, ...
International Journal of Environment and Climate Change 14 (11), 716-735 , 2024
2024
Citations: 4
Association of Different Fungal Species with the Infestation of Tea Mosquito Bug, Helopeltis antonii Signoret on Neem
B Chaitanya, M Bheemanna, A Prabhuraj, SM Murthy, HR Naik, ...
HEXAPODA, 29-34 , 2024
2024
Citations: 3
Simultaneous determination of multi-class pesticide residues in solanaceous vegetables and selected fruits using LC-MS/MS and potential impact on consumer health with …
P Aralimarad, J Bedar, S Mahato, S Hanchinal, N Naik, A Gavai, ...
2024
Citations: 1
Biology and Management of Tea Mosquito Bug, Helopeltis Antonii on Neem
B Chaitanya, M Bheemanna, A Prabhuraj, MS Murthy, NR Harischandra, ...
2024
Citations: 1
Efficacy of Biopesticides against Pod Bug, Clavigralla gibbosa Spinola (Heteroptera; Coreidae) in Pigeonpea.
BR Chethan, V Rachappa, SG Hanchinal, HR Naik, SR Doddagoudar
Legume Research: An International Journal 47 (2) , 2024
2024
Citations: 4
Investigation on the insecticidal activities of cyanobacterial extracts as an alternative source for the management of fall armyworm, Spodoptera frugiperda (J. E. Smith …
KB Sharanappa C.H., Harischandra R. Naik
Heliyon 10, e29060 , 2024
2024
Citations: 15
Efficacy of some insecticides against thrips and yellow mites in chilli
G Poornima, D Pramesh, P Ratnamma, M Saraswati, ...
Indian Journal of Entomology, 575-578 , 2024
2024
Citations: 4
Toxicity and enzymatic inhibitory action of Spirulina sp. and Nostoc muscorum extracts on detoxifying enzymes in Spodoptera frugiperda (JE Smith)(Noctuidae: Lepidoptera)
CH Sharanappa, M Bheemanna, A Prabhuraj, NR Harischandra, ...
Acta Sci. Biol. Sci 46, e68619 , 2024
2024
Citations: 3
Investigation on the insecticidal activities of cyanobacterial extracts as an alternative source for the management of fall armyworm, Spodoptera frugiperda (J. E. Smith …
KB Sharanappa CH, Harischandra R. Naik
Heliyon , 2024
2024

MOST CITED SCHOLAR PUBLICATIONS

Phosphine resistance in India is characterised by a dihydrolipoamide dehydrogenase variant that is otherwise unobserved in eukaryotes
R Kaur, M Subbarayalu, R Jagadeesan, GJ Daglish, MK Nayak, HR Naik, ...
Heredity 115 (3), 188-194 , 2015
2015
Citations: 113
Simultaneous determination of 79 pesticides in pigeonpea grains using GC-MS/MS and LC-MS/MS
NR Harischandra, MS Pallavi, M Bheemanna, K PavanKumar, ...
Food Chemistry, 128986 , 2021
2021
Citations: 106
Field evaluation of different entomopathogenic fungal formulations against sucking pests of okra.
PRH Naik, S Shekharappa
2009
Citations: 64
Performance of botanical and fungal formulation for pest management in organic okra production system
RH Naik, N Devakumar, GE Rao, N Vijaya, HSI Khan, S Subha
Journal of Biopesticides 5, 12 , 2012
2012
Citations: 54
Simultaneous determination of pesticide residues in pomegranate whole fruit and arils using LC-MS/MS
RH Naik, MS Pallavi, A Shwetha, M Bheemanna, RU Nidoni
Food chemistry 387, 132865 , 2022
2022
Citations: 36
Determination of 72 chemical pesticides and estimation of measurement of uncertainty in rice using LC-MS/MS and GC-MS/MS
R Harischandra Naik, MS Pallavi, K Pavan Kumar, BK Vanitha, ...
Food Analytical Methods 14 (9), 1788-1805 , 2021
2021
Citations: 29
Method Development and Validation for Determination of Indoxacarb Using LC-ESI-MS/MS and Its Dissipation Kinetics in Pigeonpea
N HR, MS Pallavi, R Chawan, M Bheemanna, A Naik, M Paramasivam
Food Analytical Methods 13 (3), 647-657 , 2020
2020
Citations: 29
Simultaneous determination, dissipation and decontamination of fungicides applied on cabbage using LC-MS/MS
MS Pallavi, RH Naik, U Nidoni, M Bheemanna, D Pramesh
Food chemistry 355, 129523 , 2021
2021
Citations: 26
SPLAT-PBW: An eco-friendly, cost-effective mating disruption tool for the management of the pink bollworm on cotton
BPR Sreenivas A G, Markandeya G, Harischandra Naik R, Usha R, Hanchinal S.G.
Crop Protection, https://doi.org/10.1016/j.cropro.2021.10 , 2021
2021
Citations: 25
Determination of 73 multi-class pesticides in okra (Abelmoschus esculentus L.) fruits using LC–MS/MS and GC–MS/MS and estimation of analytical uncertainty of measurement
MS Pallavi, RH Naik, K Pavankumar, A Shwetha, P Naveenkumar, ...
Food Chemistry: X 19, 100814 , 2023
2023
Citations: 23
Field evaluation of fungicides against false smut disease of rice
H Sharanabasav, D Pramesh, E Chidanandappa, A Saddamhusen, ...
J. Pharm. Phytochem 9, 1453-1456 , 2020
2020
Citations: 22
Determination of 77 Multiclass Pesticides and Their Metabolitesin Capsicum and Tomato Using GC-MS/MS and LC-MS/MS
H Naik Rathod, B Mallappa, P Malenahalli Sidramappa, ...
Molecules 26 (7), 1837 , 2021
2021
Citations: 18
Determination of chlorantraniliprole 18.5% SC in the paddy ecosystem and its risk assessment
S Mahato, RH Naik, M Bheemanna, MS Pallavi, S Hurali, SN Rao, ...
Scientific Reports 13 (1), 5464 , 2023
2023
Citations: 17
In vitro evaluation of entomopathogenic fungal formulations against sucking insect pests of okra.
RH Naik, S Shekharappa
2009
Citations: 17
Investigation on the insecticidal activities of cyanobacterial extracts as an alternative source for the management of fall armyworm, Spodoptera frugiperda (J. E. Smith …
KB Sharanappa C.H., Harischandra R. Naik
Heliyon 10, e29060 , 2024
2024
Citations: 15
Toxic effect of cyanobacterial (blue–green algae) extracts as natural pesticides for the control of Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)
CH Sharanappa, M Bheemanna, A Prabhuraj, RN Harischandra, ...
Egyptian Journal of Biological Pest Control 33 (1), 77 , 2023
2023
Citations: 14
Determination of imidacloprid residues in mulberry leaves by QuEChERS and liquid chromatography with diode array detection
M Paramasivam, S Chandrasekaran, RH Naik, P Karthik, P Thangachamy, ...
Journal of Liquid Chromatography & Related Technologies 37 (1), 122-129 , 2014
2014
Citations: 14
Genetic diversity and pathotype profiling of Xanthomonas oryzae pv. oryzae isolates from diverse rice growing ecosystems of Karnataka state of India.
A Raghunandana, D Pramesh, S Gururaj, C Amoghavarsha, MK Yadav, ...
Plant Protection Science 59 (1) , 2023
2023
Citations: 13
LC-ESI-MS/MS Method for Determination of Chlorantraniliprole Residue and its Dissipation Kinetics in Pigeonpea
PDBM Rahul Chawan, Harischandra Naik R*, Pallavi M S, Rachappa V1
Pesticide Research Journal 32 (1), 96-106 , 2020
2020
Citations: 12
Effect of biosynthesized zinc oxide green nanoparticles on pulse beetle, Callosobruchus analis (Coleoptera: Chrysomelidae)
SN Wazid, A Prabhuraj, RH Naik, NM Shakuntala, H Sharanagouda
Int J Curr Microbiol App Sci 7 (09), 503-512 , 2018
2018
Citations: 12