Upregulation of Apoptotic Markers p53 and Caspase-3 in Cardiac Tissue of Diabetic Rats Jordan Journal of Biological Sciences, 2025 Diabetes is a metabolic disorder associated with hyperglycemia.Among the various complications of diabetes, cardiovascular disease remains the leading cause of mortality in diabetic patients.This study aimed to investigate the expression of p53 and caspase-3 in the cardiac tissue of diabetic rats and to assess their association with the progression of diabetes.A single intraperitoneal injection of alloxan induced diabetes at a dose of 120 mg/kg.Rats with blood glucose levels exceeding 250 mg/dL were classified as diabetic.After the experiment, all animals were euthanized, and blood samples and cardiac tissue specimens were collected for further experiments.Biochemical assessments revealed significant increases in blood glucose, cholesterol, and triglyceride levels in the diabetic group compared to the controls.Immunohistochemical analysis showed elevated expression of both p53 and caspase-3 in the hearts of diabetic rats.Notably, the increase in caspase-3 expression was statistically significant (p = 0.001), whereas the increase in p53 expression did not reach statistical significance (p = 0.077).These findings suggest that diabetes induces cardiac stress and may activate apoptotic pathways, potentially contributing to the development of cardiovascular complications.
Secoisolariciresinol Diglucoside as a Potential Hepatoprotective Agent Against Mercuric Chloride-Induced Oxidative Damage Jordan Journal of Biological Sciences, 2025 Mercuric chloride (HgCl)-induced hepatotoxicity involves oxidative stress and apoptosis, posing a significant public health concern.Secoisolariciresinol Diglucoside (SDG), a bioactive lignan derived from flaxseed, exhibits potent antioxidant and anti-inflammatory properties.This study investigated the hepatoprotective potential of SDG in female Wistar rats exposed to HgCl.Twenty-four rats were allocated into four groups: control, HgCl (2 mg/kg, intraperitoneal), SDG (5 mg/kg, subcutaneous), and HgCl + SDG (co-administered one hour apart).Biochemical analyses included oxidative stress markers (malondialdehyde, total oxidant status, oxidative stress index), antioxidant enzymes (superoxide dismutase, catalase, glutathione peroxidase), liver function tests (ALT, AST, ALP, bilirubin), and histopathological examination.Exposure to HgCl significantly increased oxidative markers and liver enzymes while reducing antioxidant enzyme activities, indicating severe hepatic damage.Treatment with SDG significantly ameliorated these changes, restoring redox balance and preserving hepatic tissue architecture.These findings demonstrate that SDG confers hepatoprotection against HgCl-induced liver injury, likely through its antioxidant capacity and modulation of oxidative stress pathways, supporting its potential as a therapeutic agent for heavy metal-induced hepatotoxicity.
Introduction to Nutrition and Cancer Hanadi Talal Ahmedah, Haneen Adel Basheer, Inas Almazari, Kawther Faisal Amawi Cancer Treatment and Research, 2024
Evaluation of Jordan’s citizens’ awareness of the emerging Coronavirus (COVID-19) disease: A mixed analysis of the symptoms, transmission, and preventive measures Inas Almazari, Roba Bdeir, Haneen A. Basheer Plos Global Public Health, 2022 This study aimed to evaluate Jordan citizens’ awareness, knowledge, and practice concerning Coronavirus-19 (COVID-19) symptoms, routes of transmission, and preventive measures. An online self-administered questionnaire was filled out completely by participants (N = 328) from mainly four major cities in Jordan during the period beginning of May-end of September 2020. Participants’ main sources of knowledge about COVID-19 were the government websites (87.8%), social media (87.5%), and Television (TV) (81.1%). The majority of participants valued the drizzle of cough from infected individuals (96.3%), direct contact with contaminated surfaces (91.5%), and direct contact with infected individuals (84.5%) as the highest rates of the route of transmission. The highest rates chosen as symptoms of COVID-19 viral infections were high-grade fever (99.1%), troublesome breathing (96.6%), coughing (92.7%), headache (91.2%), and loss of smell and taste (80.8%). The majority of participants (>92%) strongly agreed on the behavioral protective measures such as no face touching, wearing a mask, the use of alcoholic hand disinfectants, and the need for self-isolation. This study showed that the Jordanian citizens were aware of the epidemiology of COVID-19 and related infection preventive measures. This agrees well with the efforts done by the Ministry of health and governmental organizations to spread the necessary information about the virus among citizens.
Milestones to Contemporary Perspectives MA Assab, I Almazari, Z Zakaraya, D Al-jakhim AI-Driven Healthcare: Automation and Robotics, 181 , 2026 2026
The Expression of TNF-α and IL-6: Exploring the Histological and Biochemical Antidiabetic Effects of Okra Seed on Alloxan-Induced Diabetic Model KF Amawi, I Almazari, AM Abdallatif, MA Assab, DS Al-Qaisi, ... AI-Driven Healthcare: Automation and Robotics, 515-528 , 2026 2026
The Impact of Thyroid Dysfunction on Lipid Profile: Exploring Hyperlipidemia in Thyroid Disease AAR Hashash, BA Khalaileh, RB Al-Amairyh, I Almazari, MA Alkhateeb, ... AI-Driven Healthcare: Automation and Robotics, 51-62 , 2026 2026
Tracing the Evolution of Pharmacovigilance: From Historical Milestones to Contemporary Perspectives MA Assab, I Almazari, Z Zakaraya, D Al-jakhim, HA Assab AI-Driven Healthcare: Automation and Robotics, 181-191 , 2026 2026
Diabetes Upregulates the Expression of HSP90 and Downregulates HSP70 in the Pancreatic Tissue of Rat Models with “Type 1 Diabetes” K Amawi, DM Alsholi, I Almazari, T Saleh, AS Ali Deeb, A Hamdallah, ... AI-Driven Healthcare: Automation and Robotics, 669-679 , 2026 2026
Immunophenotyping of Cells Infiltrating Pancreatic Tissue of Diabetic Rats K Amawi, I Almazari, A Aladwan, MA Assab, MA Alkhateeb AI-Driven Healthcare: Automation and Robotics, 295-307 , 2026 2026
Upregulation of Apoptotic Markers p53 and Caspase-3 in Cardiac Tissue of Diabetic Rats. K Amawi, DM Alsholi, WB Salameh, AM Abdallatif, I Almazari, ... Jordan Journal of Biological Sciences 18 (4) , 2025 2025
Effectiveness of a pharmacist-led heart failure management program in improving patient outcomes Z Zakaraya, M Abu Assab, MT Alsaadi, D Azizi, A Nowwara, I Almazari J Nat Sci Biol Med 15 (3), 513-523 , 2024 2024 Citations: 3
Introduction to nutrition and Cancer HT Ahmedah, HA Basheer, I Almazari, KF Amawi Nutrition and Dietary Interventions in Cancer, 1-32 , 2024 2024 Citations: 5
Evaluation of Jordan’s citizens’ awareness of the emerging Coronavirus (COVID-19) disease: A mixed analysis of the symptoms, transmission, and preventive measures I Almazari, R Bdeir, HA Basheer PLOS Global Public Health 2 (9), e0001041 , 2022 2022
Barriers to medication review process implementation—cross-sectional study among community pharmacists in Jordan M Abu Assab, H Alhamad, I Almazari, B Azzam, H Abu Assab Healthcare 10 (4), 651 , 2022 2022 Citations: 14
Evaluation of antioxidant activation by potential nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/Keap1 complex inhibitors IS Almazari, SY Elhayek Tropical Journal of Pharmaceutical Research 20 (9), 1861-1873 , 2021 2021 Citations: 1
Compound expression of inducible nitric oxide synthase (inos) and p53 in prostate tissue of diabetic rats IS Al-Mazari, A Alsarhan, KF Amawi, ASA Deeb, A Al-Emerieen, ... Gorteria 61 (4) , 2021 2021 Citations: 1
Research Article The Compound Expression of HSP90 and INOS in the Testis of Diabetic Rats as Cellular and Pathologic Adverse Effects of Diabetes A Alsarhan, KF Amawi, IS Al-Mazari, HA Hurirah, AJ Alkhatib 2020
The compound expression of HSP90 and INOS in the testis of diabetic rats as cellular and pathologic adverse effects of diabetes A Alsarhan, KF Amawi, IS Al-Mazari, HA Hurirah, AJ Alkhatib Analytical Cellular Pathology 2020 (1), 3906583 , 2020 2020 Citations: 13
Diabetes upregulates the expression of HSP90 and downregulates HSP70 in the liver of diabetic rats KF Amawi, IS Al-Mazari, A Alsarhan, HQM Alhamad, AJ Alkhatib Comparative Clinical Pathology 28 (2), 473-478 , 2019 2019 Citations: 21
Discovery of novel potent nuclear factor kappa‐B inhibitors (IKK‐β) via extensive ligand‐based modeling and virtual screening MA Al‐Sha'er, IS Almazari, MO Taha Journal of Molecular Recognition 30 (6), e2604 , 2016 2016 Citations: 10
"Evaluation of novel Akt1 inhibitors as anticancer agents using virtual co-crystallized pharmacophore generation" NH Mahmoud A Al-Sha’er, Iman Mansi, Inas Almazari Journal of Molecular Graphics and Modelling 62, 213-225 , 2015 2015 Citations: 25
Cancer chemopreventive and therapeutic potential of guggulsterone I Almazari, YJ Surh Natural Products in Cancer Prevention and Therapy, 35-60 , 2012 2012 Citations: 22
Nrf2-Keap1 and NF-kB-IkB Kinase Axes as Potential Chemopreventive Targets of Guggulsterone in Human Mammary Epithelial Cells IS Almazari 서울대학교 대학원 , 2012 2012
MOST CITED SCHOLAR PUBLICATIONS
Piceatannol induces heme oxygenase-1 expression in human mammary epithelial cells through activation of ARE-driven Nrf2 signaling HH Lee, SA Park, I Almazari, EH Kim, HK Na, YJ Surh Archives of biochemistry and biophysics 501 (1), 142-150 , 2010 2010.0 Citations: 76
Synthesis and Antibacterial Properties of New 8-Nitrofluoro quinolone Derivatives YM Al-Hiari, IS Al-Mazari, AK Shakya, RM Darwish, R Abu-Dahab Molecules 12 (6), 1240-1258 , 2007 2007.0 Citations: 73
Guggulsterone induces heme oxygenase-1 expression through activation of Nrf2 in human mammary epithelial cells: PTEN as a putative target I Almazari, JM Park, SA Park, JY Suh, HK Na, YN Cha, YJ Surh Carcinogenesis 33 (2), 368-376 , 2012 2012.0 Citations: 48
"Evaluation of novel Akt1 inhibitors as anticancer agents using virtual co-crystallized pharmacophore generation" NH Mahmoud A Al-Sha’er, Iman Mansi, Inas Almazari Journal of Molecular Graphics and Modelling 62, 213-225 , 2015 2015.0 Citations: 25
Cancer chemopreventive and therapeutic potential of guggulsterone I Almazari, YJ Surh Natural Products in Cancer Prevention and Therapy, 35-60 , 2012 2012.0 Citations: 22
Diabetes upregulates the expression of HSP90 and downregulates HSP70 in the liver of diabetic rats KF Amawi, IS Al-Mazari, A Alsarhan, HQM Alhamad, AJ Alkhatib Comparative Clinical Pathology 28 (2), 473-478 , 2019 2019.0 Citations: 21
Barriers to medication review process implementation—cross-sectional study among community pharmacists in Jordan M Abu Assab, H Alhamad, I Almazari, B Azzam, H Abu Assab Healthcare 10 (4), 651 , 2022 2022.0 Citations: 14
The compound expression of HSP90 and INOS in the testis of diabetic rats as cellular and pathologic adverse effects of diabetes A Alsarhan, KF Amawi, IS Al-Mazari, HA Hurirah, AJ Alkhatib Analytical Cellular Pathology 2020 (1), 3906583 , 2020 2020.0 Citations: 13
Discovery of novel potent nuclear factor kappa‐B inhibitors (IKK‐β) via extensive ligand‐based modeling and virtual screening MA Al‐Sha'er, IS Almazari, MO Taha Journal of Molecular Recognition 30 (6), e2604 , 2016 2016.0 Citations: 10
Barriers to Medication Review Process Implementation—Cross-Sectional Study among Community Pharmacists in Jordan. Healthcare. 2022; 10 (4): 651 M Abu Assab, H Alhamad, I Almazari, B Azzam, H Abu Assab Citations: 7
Introduction to nutrition and Cancer HT Ahmedah, HA Basheer, I Almazari, KF Amawi Nutrition and Dietary Interventions in Cancer, 1-32 , 2024 2024.0 Citations: 5
Effectiveness of a pharmacist-led heart failure management program in improving patient outcomes Z Zakaraya, M Abu Assab, MT Alsaadi, D Azizi, A Nowwara, I Almazari J Nat Sci Biol Med 15 (3), 513-523 , 2024 2024.0 Citations: 3
Evaluation of antioxidant activation by potential nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/Keap1 complex inhibitors IS Almazari, SY Elhayek Tropical Journal of Pharmaceutical Research 20 (9), 1861-1873 , 2021 2021.0 Citations: 1
Compound expression of inducible nitric oxide synthase (inos) and p53 in prostate tissue of diabetic rats IS Al-Mazari, A Alsarhan, KF Amawi, ASA Deeb, A Al-Emerieen, ... Gorteria 61 (4) , 2021 2021.0 Citations: 1
Milestones to Contemporary Perspectives MA Assab, I Almazari, Z Zakaraya, D Al-jakhim AI-Driven Healthcare: Automation and Robotics, 181 , 2026 2026.0
The Expression of TNF-α and IL-6: Exploring the Histological and Biochemical Antidiabetic Effects of Okra Seed on Alloxan-Induced Diabetic Model KF Amawi, I Almazari, AM Abdallatif, MA Assab, DS Al-Qaisi, ... AI-Driven Healthcare: Automation and Robotics, 515-528 , 2026 2026.0
The Impact of Thyroid Dysfunction on Lipid Profile: Exploring Hyperlipidemia in Thyroid Disease AAR Hashash, BA Khalaileh, RB Al-Amairyh, I Almazari, MA Alkhateeb, ... AI-Driven Healthcare: Automation and Robotics, 51-62 , 2026 2026.0
Tracing the Evolution of Pharmacovigilance: From Historical Milestones to Contemporary Perspectives MA Assab, I Almazari, Z Zakaraya, D Al-jakhim, HA Assab AI-Driven Healthcare: Automation and Robotics, 181-191 , 2026 2026.0
Diabetes Upregulates the Expression of HSP90 and Downregulates HSP70 in the Pancreatic Tissue of Rat Models with “Type 1 Diabetes” K Amawi, DM Alsholi, I Almazari, T Saleh, AS Ali Deeb, A Hamdallah, ... AI-Driven Healthcare: Automation and Robotics, 669-679 , 2026 2026.0
Immunophenotyping of Cells Infiltrating Pancreatic Tissue of Diabetic Rats K Amawi, I Almazari, A Aladwan, MA Assab, MA Alkhateeb AI-Driven Healthcare: Automation and Robotics, 295-307 , 2026 2026.0
