Dalia Tawfeek Hussein ElBassiony

@mans.edu.eg

Fellow of Biochemistry and Biomedical analysis/Children's Hospital/Faculty of Medicine/Mansoura University
Mansoura University

Dalia Tawfeek Hussein ElBassiony


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https://researchid.co/daliatawfeek

EDUCATION

Ph.D in Biochemistry

RESEARCH INTERESTS

Biochemistry, molecular biology, immunology, Covid 19, caner, oxidative stress, Hepatitis, sepsis
7

Scopus Publications

Scopus Publications

  • TNFSF4/OX40L and IKZF1/IKAROS Genetic Variant Associations with Egyptian Juvenile Systemic Lupus Erythematosus (JSLE)
    Zeinab R. Attia, Ahmed M. Amshawee, Ahmed Flayyih Hasan, Dalia Tawfeek Hussein, Rania A. Abd El Azeem, et al.
    Biology, 2026
    Background: The etiopathogenesis of juvenile systemic lupus erythematosus (JSLE), a complex and complicated illness, is unknown. Genetic, environmental, and dysregulated immune system responses are all thought to contribute to the disease’s etiology. Important immunological molecules that regulate different immune cells and are associated with autoimmune disorders are TNFSF4 and IKZF1. Thus, our purpose was to discover if TNFSF4 and IKZF1 mutations left the Egyptian population genetically predisposed to SLE. Methods: Using real-time polymerase chain reaction (RT-PCR), polymorphism analysis of the TNFSF4 rs1234315 C/T and IKZF1 rs11980379 C/T genes was performed on extracted DNA from JSLE patients and healthy controls. Results: TNFSF4 frequencies (rs1234315 T allele, CT, TT genotypes, dominant and recessive models) were substantially associated with a higher incidence of JSLE (p < 0.05) compared to healthy controls. Conversely, IKZF1 frequencies (rs11980379 T allele, TC, TT genotypes, and dominant model) significantly correlated with a lower incidence of JSLE. Furthermore, the TC + CC rs11980379 genotype was identified as significantly associated with lower kidney biopsy grades and a lower incidence of lupus nephritis. Conclusions: Our findings suggest that TNFSF4 and IKZF1 polymorphisms affect vulnerability to juvenile SLE.
  • DNA mismatch repair (MMR) genes expression in lung cancer and its correlation with different clinicopathologic parameters
    Mayada Saad Farrag, Heba Wagih Abdelwahab, Amr Abdellateef, Nahla Anber, Mohamed Adel Ellayeh, et al.
    Scientific Reports, 2025
    Lung cancer (LC) is a crucial rapidly developing disease. In Egypt, it is one of the five most frequent cancers. Little is known about the impact of deleted mismatch repair genes and its correlation to clinicopathological characteristics. This study evaluates immunohistochemical expression of the mismatch repair genes (PMS2), (MSH2), (MLH1) &amp; (MSH6) &amp; its correlation with clinicopathologic parameters &amp; prognosis of LC. Age was higher with lost MLH1 &amp; PMS2 but HTN was higher with lost four markers. Smoking was associated with expression of MLH1 &amp; PMS2. A progressive course was associated with lost MSH2 &amp; MSH6. Suprarenal metastasis was associated with lost all markers but bone metastasis was associated with lost MSH2 &amp; MSH6. All the markers were significantly correlated with each other, with perfect correlations between MSH6 &amp; MSH2 and between MLH &amp; PMS2. Median overall survival among cases with lost markers was significantly lower than patients with preserved markers. We recommend evaluation of the four proteins as a biomarker that could guide LC therapy. In-depth biological research is imperative to elucidate the precise roles and mechanisms of these markers. This will advance management strategies and even guide immune checkpoint inhibitor therapy for LC.
  • Neuroprotective and cognitive enhancing effects of herbecetin against thioacetamide induced hepatic encephalopathy in rats via upregulation of AMPK and SIRT1 signaling pathways
    Ahmed A. Sedik, Dalia T. Hussein, Khaled Fathy, Noha A. Mowaad
    Scientific Reports, 2024
    Acute liver injury, there is a risky neurological condition known as hepatic encephalopathy (HE). Herbacetin is a glycosylated flavonoid with many pharmacological characteristics. The purpose of this study was to assess the ability of herbacetin to protect against the cognitive deficits associated with thioacetamide (TAA) rat model and delineate the underlying behavioral and pharmacological mechanisms. Rats were pretreated with herbacetin (20 and 40 mg/kg) for 30days. On 30th day, the rats were injected with TAA (i.p. 350 mg/kg) in a single dose. In addition to a histpathological studies, ultra-structural architecture of the brain, liver functions, oxidative stress biomarkers, and behavioral tests were evaluated. Compared to the TAA-intoxicated group, herbacetin improved the locomotor and cognitive deficits, serum hepatotoxicity indices and ammonia levels. Herbacetin reduced brain levels of malodialdeyde, glutamine synthetase (GS), tumor necrosis factor- alpha (TNF-α), interleukin 1 B (IL-1β), annexin v, and increased brain GSH, Sirtuin 1 (SIRT1), and AMP-activated kinase (AMPK) expression levels. Also, herbacetin improve the histopathological changes and ultra- structure of brain tissue via attenuating the number of inflammatory and apoptotic cells. Herbacetin treatment significantly reduced the toxicity caused by TAA. These findings suggest that herbacetin might be taken into account as a possible neuroprotective and cognitive enhancing agent due to its ability to reduce oxidative stress, inflammation and apoptosis associated with TAA.
  • Sufficient vitamin D is favorable for children with persistent and chronic immune thrombocytopenia
    Rafae El Mabrouk, Dalia Tawfeek Hussein, Mohammed Ezz El Regal Abbas, Suzy Abd El Mabood
    Annals of Hematology, 2023
  • Involvement of INF-γ functional single nucleotide polymorphism +874 T/A (rs2430561) in breast cancer risk
    Hanan E Al-Rashidi, Sherif Refaat, Enas Ahmed, Dalia T Hussein, Fatma M Eltantawy, et al.
    Saudi Journal of Biological Sciences, 2021
  • Study of hepatitis c genotypes in serum and peripheral blood mononuclear cells in children with chronic hepatitis c infection
    Fathi El-Taweel, Mohammed El-Regal-Abbas, Raida Yahya, Walaa Nouh, Dalia Hussein
    Clinical Laboratory, 2021
  • Adipocytokines in patients with chronic kidney disease stage 5
    Raida Yahya, Mohammed Atwa, Ibrahim El-Sayed, Doaa El-Ghanaam, Dalia Hussein, et al.
    Clinical Laboratory, 2016
    BACKGROUND Chronic kidney disease (CKD) leading to kidney failure and end stage renal disease (ESRD) is a common health problem associated with wasting syndrome characterized by inadequate nutrient intake and decrease tissue anabolism and/or catabolism. In CKD adipokines, especially leptin and adiponectin (ADPN), accumulate in serum due to reduced renal clearance. Although, rapidly growing, knowledge of adipocytokines is limited and much is still unknown of the altered adipocytokine pattern in patients with impaired renal function. The aim of this study is to assess the adipocytokines, leptin, and adiponectin in relation to weight loss in pediatric patients with CKD stage-5 treated conservatively (CT) or undergoing maintenance hemodialysis (MHD). METHODS 41 CKD stage-5 patients and 20 healthy controls were included in this study. Serum levels of leptin and adiponectin were determined by ELISA. Leptin gene expression was analyzed using quantitative real time-polymerase chain reactions (QPCR). RESULTS Patients had significantly elevated ADPN levels and non significantly elevated serum leptin levels as compared to controls (p < 0.001, p = 0.354, respectively). Leptin gene expression and body mass index (BMI) were highly significantly reduced in CKD stage-5 compared to controls (p < 0.001 for each). There were no significant differences between patients treated conservatively and those undergoing MHD with respect to all studied parameters. Finally, univariate logistic regression analysis revealed no association between leptin, ADPN, and weight loss in CKD stage-5 patients. CONCLUSIONS The present study showed non significantly elevated or even normalized serum leptin levels, elevated serum adiponectin level and reduced leptin gene expression in CKD stage-5 patients as compared to healthy controls. Patients had significantly lower weight than healthy controls but there was no association between leptin, adiponectin, and weight loss in CKD stage-5 studied patients so, further studies are needed to clarify the role of the two adipokines in body weight loss in those patients.