Plant growth-promoting rhizobacteria for controlling rice diseases: What is known and what is next? Nazla Kayla, Dedat Prismantoro, Muhammad Adil Awal, Thomas Argyarich Jefferson, Yolani Syaputri, Raden Salma Nauraqurota'ayun Azzahra, Anggita Rahmi Hafsari, Mia Miranti, Ravindra Chandra Joshi, Febri Doni Journal of Agriculture and Food Research, 2026 Rice ( Oryza sativa ) is a global staple threatened by diseases such as bacterial leaf blight ( Xanthomonas oryzae pv. oryzae ), sheath blight ( Rhizoctonia solani ), blast ( Magnaporthe oryzae ), and panicle blight ( Burkholderia glumae and B. gladioli ), which cause significant yield losses. Conventional agrochemical use has raised environmental and health concerns, prompting a shift toward sustainable alternatives. This review examines the role of plant growth-promoting rhizobacteria (PGPR) as promising biocontrol agents. PGPR enhance rice health and suppress pathogens through a variety of direct and indirect mechanisms. Direct actions include the production of antibiotics and lytic enzymes, competition for nutrients, and the synthesis of phytohormones and siderophores. Indirectly, PGPR activate induced systemic resistance (ISR), priming the plant's immune system via jasmonic acid (JA) and ethylene (ET) signaling pathways. While empirical studies confirm that PGPR can improve plant growth, enhance stress tolerance, and reduce disease severity, inconsistent field performance remains a major challenge. This variability is often due to complex strain-environment interactions, inconsistent root colonization, and the lack of robust formulations. To overcome these limitations, future research should focus on utilizing 'omics-based approaches to better understand PGPR-microbe-plant interactions. Additionally, there is a need to develop more stable biostimulant consortia and to fully integrate these biological agents into climate-smart agricultural systems. This review synthesizes current knowledge on PGPR-mediated disease management and highlights their potential to improve crop resilience and productivity while promoting eco-friendly farming practices. • PGPR suppress rice diseases through direct and indirect biocontrol mechanisms. • Induced systemic resistance is activated via jasmonic acid and ethylene pathways. • Field performance of PGPR remains inconsistent due to strain–environment interactions. • Omics-based approaches can uncover PGPR–microbe–plant interactions, enabling more effective applications. • Stable PGPR consortia can support eco-friendly and climate-smart rice production.
Removal of cyanobacterial harmful algal blooms (HABs) from contaminated local park lake using Ganoderma lucidum mycelial pellets Zarimah Mohd Hanafiah, Anggita Rahmi Hafsari, Malini Elango, Zul Ilham, Febri Doni, Wan Hanna Melini Wan Mohtar, Yusufjon Gafforov, Yong Jie Wong, Khairul Nizam Abdul Maulud, Nor Hidayah Ismail, Mohd Yusmiaidil Putera Mohd Yusof, Wan Abd Al Qadr Imad Wan-Mohtar Heliyon, 2025 pellets, in contrast to the smooth surface of control pellets. These novel culture technologies show great promise as bio-flocculating agents for removing blue-green algae and potentially be adapted for microalgae harvesting in biodiesel production.
Performance of hydrolytic enzymes produced by Trichoderma in sustainable crop production: Current insights and future perspectives Muhammad Haikal Algifari, Dedat Prismantoro, Anggita Rahmi Hafsari, Mia Miranti, Wan Abd Al Qadr Imad Wan-Mohtar, Abdullah Bilal Ozturk, Azwir Anhar, Zul Ilham, Ravindra Chandra Joshi, Febri Doni Sustainable Environment, 2025 The fungal genus Trichoderma is widely recognized for its diverse agricultural applications, particularly its ability to enhance plant growth, suppress pathogens, facilitate organic matter decomposition and nutrient cycling. Trichoderma-produced enzymes play a crucial role in these processes, making them valuable biocatalysts for sustainable crop production. However, despite their well-documented benefits, the full potential of these enzymes remains underexplored. A deeper understanding of the molecular mechanisms governing Trichoderma’s enzymatic activity in agricultural systems could provide critical insights into optimizing their efficacy. To this end, this review examines the current applications of Trichoderma-derived enzymes in sustainable agriculture, highlights the challenges associated with their industrial-scale use, and explores emerging technologies such as genetic engineering, enzyme engineering and design, and innovative enzyme production strategies. Addressing these challenges and leveraging advanced biotechnological tools will allow Trichoderma-produced enzymes to be further optimized for broader agricultural applications, contributing to sustainable food production and environmental conservation.
Potential bacteria capable of remediating mercury contaminated soils R C B Ginting, N Solihat, A R Hafsari, Irawan Iop Conference Series Earth and Environmental Science, 2021 Abstract Mercury content in ex-artisanal and small-scale gold mining areas in Cianjur District, Province of West Java, Indonesia was 7 to 36 mg L −1 . It has exceeded the threshold value for industrial land. Bioremediation of mercury using mercury-resistant bacteria is attractive to remove mercury from the environment because it is more effective and less expensive. The objective of this study was to obtain potential bacteria capable of accumulating mercury to be used to remediate mercury contaminated soils in ex-gold mining areas. Potential bacteria isolates were characterized for their phenotypic and biochemical properties using the Biolog system. Thirty-two mercury-resistant bacteria were successfully isolated from the rhizosphere of Pityrogramma tartarea growing predominantly around tailings of ex-artisanal gold mining. After screening the presence of mercury, the three best isolates showing high resistances are Pseudomonas putida R2.13 and P. maculicola R4.27 that are capable to tolerate 180 mg L −1 mercury, and Enterobacter aerogenes R3.24 that is capable to survive at 170 mg L −1 . Furthermore, the three bacteria also can fix atmospheric nitrogen and solubilize phosphate, but they cannot solubilize potassium. These indicate that P. maculicola R4.27, P. putida R2.13, and E. aerogenes R3.24 are potential as bioaccumulation agents on mercury-contaminated soils.
Molecular Identification and Phylogenic Analysis of Phosphate Solubilizing Bacteria Aneurinibacillus migulanus from Rhizosphere Imperata Cylindrica, Karst Citatah, Bandung Barat, Jawa Barat, Indonesia A R Hafsari, L Purnawan Journal of Physics Conference Series, 2020 Abstract Phosphate converted from insoluble phosphate bonds (through secretion of organic acids) to P-inorganic bonds by phosphate solubilizing bacteria (PSB). Two highly potential PSB isolates, BR.5 and BR.7, were isolated from rhizosphere Imperata cylindrica , ex-mining limestone land in Karst Citatah, Province Jawa Barat, Indonesia. From the screening of P-solubilizing test results, BR.5 showed the highest clear zones in pikovskaya agar containing Ca 3 (PO 4 ) 2 . The molecular methods to determine the species of bacteria are DNA extraction, DNA amplification by PCR, qualitative DNA testing by electrophoresis, and sequencing. The result of PCR results of DNA bands by electrophoresis, BR.5 has 1500 bp fragment length, 16S rRNA gene yielded on the phylogenic tree of Isolate BR. 5 has a similarity or relationship with the species Aneurinibacillus migulanus Isolate Am25 with a bootstrap value of 99%. Based on the genetic distance between Isolate BR.5 and Aneurinibacillus migulanus , Isolate Am25 has a distance value of 0.71%.
RECENT SCHOLAR PUBLICATIONS
Plant growth-promoting fungi (PGPF) for controlling rice diseases: a sustainable approach TA Jefferson, M Miranti, MA Awal, AR Hafsari, D Prismantoro, RC Joshi, ... Cogent Food & Agriculture 12 (1), 2610015 , 2026 2026 Citations: 3
The Role of Mushrooms in Breaking Down Pollutants and Keeping the Environment Clean ZM Hanafiah, AR Hafsari, WHMW Mohtar, TNBT Ibrahim, NAS Feisal, ... Advances in Mushroom Research: Breakthroughs and Future Directions, 383-414 , 2026 2026
Plant growth-promoting rhizobacteria for controlling rice diseases: What is known and what is next? N Kayla, D Prismantoro, MA Awal, TA Jefferson, Y Syaputri, RSN Azzahra, ... Journal of Agriculture and Food Research, 102834 , 2026 2026
Biotechnological potential of Ganoderma species in mycoremediation: a bibliometric analysis AR Hafsari, F Doni, D Prismantoro, TA Jefferson, MA Ramdhani, NIM Ali, ... Folia Microbiologica, 1-21 , 2026 2026
Performance of hydrolytic enzymes produced by Trichoderma in sustainable crop production: Current insights and future perspectives MH Algifari, D Prismantoro, AR Hafsari, M Miranti, WAAQI Wan-Mohtar, ... Sustainable Environment 11 (1), 2593746 , 2025 2025 Citations: 6
Applying the Theory of Planned Behavior to Understand Universiti Malaya Students’ Intent to Participate in Sustainability Initiatives NASM Huzer, AR Hafsari, Z Ilham The Journal of Indonesia Sustainable Development Planning 6 (3), 359-370 , 2025 2025
Removal of Cyanobacterial Harmful Algal Blooms (HABs) from Contaminated Local Park Lake using Ganoderma lucidum Mycelial Pellets ZM Hanafiah, AR Hafsari, M Elango, Z Ilham, F Doni, WHMW Mohtar, ... Heliyon , 2025 2025 Citations: 8
KOMPOSISI BIOFERTILIZER CAIR DARI KONSORSIUM MIKROBA PELARUT FOSFAT Trichosporon asahii DAN Pseudomonas sp. UNTUK MEMPERCEPAT PRODUKSI BUAH DAN SAYUR AR Hafsari IN Patent S00,202,403,744 , 2024 2024
The CH 3 CO 2 H effect on the quality of halal gelatin from gouramy ( Osphronemus gouramy ) bone AR Hafsari, AA Wardah AIP Publishing LLC 2646 (1), 020002 , 2023 2023
Microbial fuel cell (MFC) by electroactive bacteria as a renewable electrical energy source in Indonesia NF Octaviani, N Kartika, AR Hafsari Indonesian Journal of Environmental Sustainability 1 (2), 105-113 , 2023 2023 Citations: 2
GENETIKA DAN BIOLOGI REPRODUKSI NIP Sari, AR Hafsari, S Roswandani, A Noviantari, R Hidana, S Setyowati, ... CV WIDINA MEDIA UTAMA , 2022 2022 Citations: 1
Karakteristik pH kultur kombucha teh hitam dengan jenis gula berbeda pada fermentasi batch-culture AR Hafsari, WN Farida Gunung Djati Conference Series 6, 228-232 , 2021 2021 Citations: 22
Proceeding of Mulawarman Pharmaceuticals Conferences EPAS Kuantitatif, K pada Pasien, BDRA Wahab, EN Sihite, ... 2021
The Ability of Entomopathogenic Fungus as Biocontrol of Periplaneta americana AR Hafsari, AN Hidayah 2021
Pelatihan Pembuatan Hand Sanitizer kepada Masyarakat Desa Tangguh di Kp. Pasir Honje, Ds. Sukawening Kec. Ciwidey Kab. Bandung ID Kurniawan, Y Suryani, AR Hafsari, MA Salim, T Cahyanto, A Adawiyah, ... Jurnal Surya Masyarakat 4 (1), 69-79 , 2021 2021 Citations: 1
Bioteknologi teori dan aplikasi S Darmayani, R Hidana, A Sa’diyah, PL Isrianto, H Hidayati, D Jumiarni, ... CV WIDINA MEDIA UTAMA , 2021 2021 Citations: 7
Potential of n-Hexane and Ethanol Extract from Betel Leaves (Piper betle Linn) as a Mouth Remover Caused by Bacteria Staphylococcus aureus N Kurniasih, A Junitasari, LN Nurjanah, AR Hafsari Proceeding of Mulawarman Pharmaceuticals Conferences 13, 254-260 , 2021 2021
Potential bacteria capable of remediating mercury contaminated soils RCB Ginting, N Solihat, AR Hafsari, Irawan IOP Conference Series: Earth and Environmental Science 648 (1), 012136 , 2021 2021 Citations: 10
Characterization of A Raw Starch Degrading Alpha Amylase From The Rhizosphere Soil Citatah Karst Region Bacillus sp. K2Br5 T Rosahdi, TN Azizah, F Hidayati, R Syifa, R Khoerunnisa, G Lenggana, ... Proceedings of the 1st International Conference on Islam, Science and … , 2021 2021 Citations: 1
Molecular identification of Phosphate-Solubilizing Yeast isolate KR. 1BP. 4 from Citatah karst area AR Hafsari, FR Khoerunnissa Proceedings of the 1st International Conference on Islam, Science and Technology , 2021 2021
MOST CITED SCHOLAR PUBLICATIONS
Isolasi dan identifikasi kapang endofit dari tanaman obat surian (Toona sinensis) AR Hafsari Istek 7 (2), 175-191 , 2013 2013 Citations: 24
Karakteristik pH kultur kombucha teh hitam dengan jenis gula berbeda pada fermentasi batch-culture AR Hafsari, WN Farida Gunung Djati Conference Series 6, 228-232 , 2021 2021 Citations: 22
Pengaruh Penambahan Gliserol dan Kitosan Terhadap Karakteristik Edible Film dari Kombucha Teh Hijau (Camelia Sinensis L.) AK Apriliani, AR Hafsari, Y Suryani Proceeding Biology Education Conference 16 (1), 275-279 , 2019 2019 Citations: 11
Potential bacteria capable of remediating mercury contaminated soils RCB Ginting, N Solihat, AR Hafsari, Irawan IOP Conference Series: Earth and Environmental Science 648 (1), 012136 , 2021 2021 Citations: 10
Pelatihan inovasi produk pangan abon pepaya muda di Dusun Pamagersari Desa Tanjungsari Sumedang A Widiana, T Cahyanto, A Supriatna, A Astuti, AR Hafsari, Y Suryani, ... http://ejurnal. budiutomomalang. ac. id/index. php/pambudi/article/view/538 , 2019 2019 Citations: 10
Removal of Cyanobacterial Harmful Algal Blooms (HABs) from Contaminated Local Park Lake using Ganoderma lucidum Mycelial Pellets ZM Hanafiah, AR Hafsari, M Elango, Z Ilham, F Doni, WHMW Mohtar, ... Heliyon , 2025 2025 Citations: 8
Bioteknologi teori dan aplikasi S Darmayani, R Hidana, A Sa’diyah, PL Isrianto, H Hidayati, D Jumiarni, ... CV WIDINA MEDIA UTAMA , 2021 2021 Citations: 7
Identifikasi spesies isolat bakteri k2br5 dari tanah karst dengan sistem kekerabatan melalui analisis urutan nukleotida gen 16s rrna TD Rosahdi, N Tafiani, AR Hafsari Jurnal Ilmu Kimia dan Terapan 5 (2), 84-88 , 2018 2018 Citations: 7
Isolasi dan identifikasi kapang pelarut fosfat dari fosfat guano gua pawon AR Hafsari Biota 10 (2), 165-180 , 2017 2017 Citations: 7
Performance of hydrolytic enzymes produced by Trichoderma in sustainable crop production: Current insights and future perspectives MH Algifari, D Prismantoro, AR Hafsari, M Miranti, WAAQI Wan-Mohtar, ... Sustainable Environment 11 (1), 2593746 , 2025 2025 Citations: 6
Molecular identification and phylogenic analysis of phosphate solubilizing bacteria Aneurinibacillus migulanus from rhizosphere imperata cylindrica, Karst Citatah, Bandung … AR Hafsari, L Purnawan Journal of Physics: Conference Series 1665 (1), 012006 , 2020 2020 Citations: 5
Pelatihan Inovasi Produk Pangan Berbahan Baku Singkong di Kecamatan Lembang Kabupaten Bandung Barat T Cahyanto, A Supriyatna, U Julita, A Kusumorini, AR Hafsari, Y Suryani, ... Bagimu Negeri: Jurnal Pengabdian Kepada Masyarakat 2 (1) , 2018 2018 Citations: 5
The effect of hydrochloric acid (HCl) concentration on the quality of gourami bone gelatin (Ospheronemus Gouramy Lac) AR Hafsari, D Rosmiati, D Jamaludiin Proc.: 1st International Conference on Recent Innovations (ICRI): 2983-2989 … , 2018 2018 Citations: 4
Pengaruh penggunaan media alternatif terhadap pertumbuhan F0 dan senyawa metabolit sekunder pada jamur tiram merah muda (pleurotus flabellatus) AR Hafsari, P Andriani, Y Suryani Jurnal ilmiah biologi BIOTIKA http://jurnal. unpad. ac. id/biotika/article … , 2017 2017 Citations: 4
Plant growth-promoting fungi (PGPF) for controlling rice diseases: a sustainable approach TA Jefferson, M Miranti, MA Awal, AR Hafsari, D Prismantoro, RC Joshi, ... Cogent Food & Agriculture 12 (1), 2610015 , 2026 2026 Citations: 3
Penggunaan bahan herbal dan pendekatan spritual untuk mencegah stress selama karantina COVID-19 AR Hafsari, A Saepulah, A Widiana, RA Ulfa Digital Library UIN Sunan Gunung Djati Bandung , 2020 2020 Citations: 3
Potensi Ekstrak n-Heksana dan Ekstrak Etanol dari Daun Sirih (Piper betleLinn) Sebagai Penghilang Bau Mulut yang Disebabkan BakteriStaphylococcus aureus N Kurniasih, A Junitasari, L Nurjanah, AR Hafsari Proceeding of Mulawarman Pharmaceuticals Conferences, April 2021, 135-138 , 2019 2019 Citations: 3
Microbial fuel cell (MFC) by electroactive bacteria as a renewable electrical energy source in Indonesia NF Octaviani, N Kartika, AR Hafsari Indonesian Journal of Environmental Sustainability 1 (2), 105-113 , 2023 2023 Citations: 2
GENETIKA DAN BIOLOGI REPRODUKSI NIP Sari, AR Hafsari, S Roswandani, A Noviantari, R Hidana, S Setyowati, ... CV WIDINA MEDIA UTAMA , 2022 2022 Citations: 1
Pelatihan Pembuatan Hand Sanitizer kepada Masyarakat Desa Tangguh di Kp. Pasir Honje, Ds. Sukawening Kec. Ciwidey Kab. Bandung ID Kurniawan, Y Suryani, AR Hafsari, MA Salim, T Cahyanto, A Adawiyah, ... Jurnal Surya Masyarakat 4 (1), 69-79 , 2021 2021 Citations: 1
