Optimization of Secondary Metabolite Production in the Sponge Associated Bacterium Roseivirga sp. PAP.19 Using the OSMAC Approach Andin Syamsi Pradani, Harwoko Harwoko, Maria Dyah Nur Meinita, Riviani Riviani, Ute Mettal, Riyanti Jurnal Ilmiah Perikanan Dan Kelautan, 2026 Graphical Abstract Highlight Research Compounds produced by Roseivirga PAP.19 bacteria have potential as antimicrobials. The OSMAC approach was used successfully to activate BGCs of Roseivirga PAP.19. The use of medium in bacterial culture affects the secondary metabolites produced by Roseivirga PAP.19 bacteria. Bacteria cultured in A1Bfe+C medium produced more secondary metabolites compared to the other medium. The different incubation times during culture affect the amount of secondary metabolite compounds produced. Abstract Marine sponge-associated bacteria are a rich source of bioactive metabolites. The biochemical interaction between bacteria and sponges enables symbiotic bacteria to produce metabolites similar to those of their host. Metabolite extracts from symbiotic bacteria contain bioactive compounds with cytotoxic, antiviral, and antimicrobial properties. There is an urgent need for new antimicrobial agents due to the increasing resistance to existing drugs. This study aims to optimize the medium and incubation time of secondary metabolites produced by the symbiotic bacteria of the sponge Roseivirga sp. PAP.19. To stimulate biosynthesis of silent gene clusters, the One Strain Many Compounds (OSMAC) approach was used. Bacteria were cultured in various media and at different incubation times. This has the potential to increase the production of secondary metabolites. Secondary metabolites were analyzed using LC-HRMS. The potential of these metabolites as antimicrobial agents was subsequently evaluated. Results showed six compounds with antimicrobial potential. Notably, the A1BFe+C medium produced more bioactive compounds than the other medium, and the highest compound production occurred on day 4. These findings suggest that optimizing culture conditions using the OSMAC approach can enhance the yield of potentially valuable antimicrobial secondary metabolites from symbiotic bacteria, which may contribute to the discovery of new antimicrobial agents.
Antimalarial Activity Screening from Endophytic Fungus of Red Ginger (Zingiber officinale): in vitro and in silico Studies Muh Ade Artasasta, Diandra Mytha Faradilla, Herlina Rasyid, Dwi Listyorini, Wira Eka Putra, et al. Sains Malaysiana, 2025 Diversity exploration of secondary metabolite compounds from plant endophytic fungi is expected to yield novel compounds that can efficiently overcome Plasmodium resistance. This study aimed to assess the antimalarial activity of the endophytic fungus derived from red ginger Zingiber officinale against Plasmodium berghei. Subsequently, the endophytic fungus was isolated from the sample using the dilution method, followed by evaporation. Nine isolates of endophytic fungi were successfully isolated that were assigned as JMR1, JMR2, JMR3, JMR5, JMB1, JMB2, JMB3, JMD1, and JMD2. The antimalarial efficacy showed that the JMR5 isolate exhibited significant activity in suppressing the proliferation of P. berghei. This activity was quantified by a per cent inhibition of 83.01% and an IC50 value of 5.81 µg/mL. The detected antimalarial activity of the JMR5 extract can be related to the presence of several phytochemicals, including alkaloids, flavonoids, and terpenoids. In addition, molecular identification was conducted using ITS primers on the JMR5 isolate, showing a complete genetic similarity of 100% with Aspergillus flavus. GNPS analysis was conducted using LCMS-MS data on ethyl acetate extract. Surafactin c, surafactin c14 and erucamide were probably secondary metabolites in the JMR5 extract. Furthermore, drug-likeness and molecular docking analysis were conducted. The result showed that erucamide is a potential antimalarial due to the fulfil of Lipinski's rule of five and also the binding affinity (- 4.2 kcal/mol) against Plasmepsin I. Based on the results obtained, the development of secondary metabolites from Aspergillus flavus JMR5 as potential antimalarial compounds is important to carry out.
Molecular Networking Analysis and Antibacterial Potential of Ethyl Acetate Extracts of Sinomicrobium sp. PAP.21 using OSMAC Method Harwoko Harwoko, Anggun Tri Rahmawati, Riyanti Riyanti Majalah Obat Tradisional, 2024 Challenges in drug discovery include biosynthetic gene clusters which remain silent under standard laboratory culture conditions. On the other hand, the rediscovery of the known compounds is inevitable. Accordingly, One Strain-MAny Compounds (OSMAC) approach and molecular networking analysis are currently applicable to discovering new bioactive compounds. Sinomicrobium sp. PAP.21 isolated from marine sediment collected in Cenderawasih Bay, West Papua, was added to the culture. Then, the bacterium was cultured in five different liquid media (RL1, A1BFe+C, NB, LB, and seawater) and incubated for 4, 5, and 7 days. The bacterial cultures were extracted using ethyl acetate (EtOAc) separately for each medium and incubation period, followed by LC-HRMS measurement. A total of 45 ethyl acetate extracts were assayed for in vitro antibacterial activity against Micrococcus luteus and Escherichia coli. Molecular networking analysis through GNPS indicated that three putative compounds possess antibacterial properties. EtOAc extracts from the A1BFe+C medium demonstrated antibacterial activity against M. luteus. However, none of them were active against E. coli. Collectively, Sinomicrobium sp. PAP.21 produced bioactive compounds exhibiting antibacterial potential, particularly against Gram-positive bacteria.
Draft Genome Sequences of Algoriphagus sp. Strain PAP.12 and Roseivirga sp. Strain PAP.19, Isolated from Marine Samples from Papua, Indonesia Riyanti, Celine M. Zumkeller, Marius Spohn, Sanja Mihajlovic, Oliver Schwengers, Alexander Goesmann, Nur A. Choironi, Till F. Schäberle, Harwoko Harwoko Microbiology Resource Announcements, 2023 Algoriphagus sp. strain PAP.12 (EXT111900) and Roseivirga sp. strain PAP.19 (EXT111901) were isolated from marine samples. Here, we report their draft genome sequences, 5.032 Mbp and 4.583 Mbp in size, respectively, and rate their specialized metabolite production capacity. Taxonomic ranks established by genome-based analysis indicate that Algoriphagus sp. strain PAP.12 represents a candidate new species.
Draft Genome Sequence of Sinomicrobium sp. Strain PAP.21, Isolated from a Coast Sample of Papua, Indonesia Riyanti, Celine M. Zumkeller, Marius Spohn, Sanja Mihajlovic, Oliver Schwengers, Alexander Goesmann, Riviani Riviani, Maria D. N. Meinita, Till F. Schäberle, Harwoko Harwoko Microbiology Resource Announcements, 2023 Sinomicrobium sp. strain PAP.21 (EXT111902) was isolated from the coast of Cenderawasih Bay National Park in West Papua, Indonesia. Its genome was assembled into 151 contigs with a total size of 5.439 Mbp, enabling the prediction of its specialized metabolite production capacity.
Formulation and Evaluation of "Nata de Curcuma" Annisa Auliya Rahmah, Atika Putri, Felicia Ivena, Monica Ramadhanti, Harwoko Harwoko Aip Conference Proceedings, 2023 Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Share Icon Share Twitter Facebook Reddit LinkedIn Tools Icon Tools Reprints and Permissions Cite Icon Cite Search Site Citation Annisa Auliya Rahmah, Atika Putri, Felicia Ivena, Monica Ramadhanti, Harwoko Harwoko; Formulation and evaluation of "Nata de Curcuma". AIP Conference Proceedings 24 January 2023; 2586 (1): 030001. https://doi.org/10.1063/5.0106928 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAIP Publishing PortfolioAIP Conference Proceedings Search Advanced Search |Citation Search
Dithiodiketopiperazine derivatives from endophytic fungi Trichoderma harzianum and Epicoccum nigrum Harwoko Harwoko, Georgios Daletos, Fabian Stuhldreier, Jungho Lee, Sebastian Wesselborg, Michael Feldbrügge, Werner E. G. Müller, Rainer Kalscheuer, Elena Ancheeva, Peter Proksch Natural Product Research, 2021 A new epidithiodiketopiperazine (ETP), pretrichodermamide G (1), along with three known (epi)dithiodiketopiparazines (2-4) were isolated from cultures of Trichoderma harzianum and Epicoccum nigrum, endophytic fungi associated with medicinal plants Zingiber officinale and Salix sp., respectively. The structure of the new compound (1) was established on the basis of spectroscopic data, including 1D/2D NMR and HRESIMS. The isolated compounds were investigated for their antifungal, antibacterial and cytotoxic potential against a panel of microorganisms and cell lines. Pretrichodermamide A (2) displayed antimicrobial activity towards the plant pathogenic fungus Ustilago maydis and the human pathogenic bacterium Mycobacterium tuberculosis with MIC values of 1 mg/mL (2 mM) and 25 µg/mL (50 µM), respectively. Meanwhile, epicorazine A (3) exhibited strong to moderate cytotoxicity against L5178Y, Ramos, and Jurkat J16 cell lines with IC50 values ranging from 1.3 to 28 µM. Further mechanistic studies indicated that 3 induces apoptotic cell death. Graphical Abstract
Azacoccones F-H, new flavipin-derived alkaloids from an endophytic fungus Epicoccum nigrum MK214079 Harwoko Harwoko, Jungho Lee, Rudolf Hartmann, Attila Mándi, Tibor Kurtán, Werner E.G. Müller, Michael Feldbrügge, Rainer Kalscheuer, Elena Ancheeva, Georgios Daletos, Marian Frank, Zhen Liu, Peter Proksch Fitoterapia, 2020 Three new flavipin-derived alkaloids, azacoccones F-H (1-3), along with six known compounds (4-9) were isolated from the endophytic fungus Epicoccum nigrum MK214079 associated with leaves of Salix sp. The structures of the new compounds were established by analysis of their 1D/2D nuclear magnetic resonance (NMR) and high-resolution electrospray ionization mass spectroscopy (HRESIMS) data. The absolute configuration of azacoccones F-H (1-3) was determined by comparison of experimental electronic circular dichroism (ECD) data with reported ones and biogenetic considerations. Epicocconigrone A (4), epipyrone A (5), and epicoccolide B (6) exhibited moderate antibacterial activity against Staphylococcus aureus ATCC 29213 with minimal inhibitory concentration (MIC) values ranging from 25 to 50 μM. Furthermore, epipyrone A (5) and epicoccamide A (7) displayed mild antifungal activity against Ustilago maydis AB33 with MIC values of 1.6 and 1.8 mM, respectively. Epicorazine A (8) showed pronounced cytotoxicity against the L5178Y mouse lymphoma cell line with an IC50 value of 1.3 μM.
Phytochemical analysis and evaluation of purified extract of Tinospora crispa stem for in vivo antihyperuricemic effect Harwoko Harwoko, Warsinah Warsinah Journal of Reports in Pharmaceutical Sciences, 2020 Background: Tinospora crispa is used in folk medicines for the treatment of gout, rheumatoid arthritis, and internal inflammation. The presence of flavonoids, polyphenols, glycosides, and alkaloids in T. crispa stem is supposed to contribute to these therapeutic effects. This study aimed to analyze qualitative and quantitative phytochemical of purified extract of T. crispa stem (PETS) and to evaluate the in vivo antihyperuricemic effect. Materials and Methods: First, total flavonoid and total alkaloid contents of PETS were determined by colorimetric and gravimetric methods. After that, potassium oxonate-induced hyperuricemic mice were treated with three doses of PETS at 50, 100, and 200 mg/kg, and hydroalcoholic extract at 500 mg/kg. Moreover, allopurinol at 10 mg/kg and sodium carboxymethylcellulose 0.5% were orally administered as positive and negative controls, respectively. Serum uric acid levels were measured by ultraviolet–visible spectrophotometry. Results: The high flavonoids content (31.08% ± 1.77% rutin equivalent) in T. crispa stem possesses a potential as uricostatic in the treatment of gout. The purified extract of T. crispa stem at a dose of 100 mg/kg revealed a significant uric acid–lowering effect compared with negative control (P < 0.05). Conclusion: This study indicates the potential of T. crispa purified extracts in the treatment of hyperuricemia and gout.
Phytochemical analysis and antioxidant activity of brotowali (Tinospora crispa l. mier) stem Warsinah Warsinah, Hanif Nasiatul Baroroh, Harwoko Harwoko Molekul, 2020 Free radical in the body could cause degenerative diseases such as diabetes mellitus and hyperlipidemia, which could be prevented by the supplementation of antioxidant whether it is synthetic or natural. Flavanoids is a phenolic compound, was extracted from natural sources is known for its antioxidative potential. This research aimed to investigate the active compounds in brotowali (Tinospora crispa) stem to find new antioxidative potential using its ability to bind 2, 2’-diphenyl-1-picrylhydrazyl (DPPH). Brotowali stem was extracted using ethanol and fractioned using n-hexane, ethyl acetate, and water. Water fraction was hydrolyzed by chloric acid. The antioxidant activity of ethanol extract, water fraction, and hydrolized water fraction (subfraction I and subfraction II) were determined using spectrophotometry (DPPH methods). The compound of flavonoid was identified using KLT with AlCl3 reagent spray. The results showed that ethanol extract and water fraction of brotowali stem contained flavonoid glycoside of flavonol group. Antioxidant activity, quantified using IC50, of ethanol extract, water fraction, subfraction I and subfractiont II were 49.92μg/mL, 38.25 μg/mL, 36.12 μg/mL, and 16.18 μg/mL, respectively. In addition, this research was found that hydrolysis of water fraction using chloric acid like in subfraction I and subfraction II was able to improve its antioxidant activity.
Cyclic heptapeptides from the soil-derived fungus Clonostachys rosea Nada M. Abdel-Wahab, Harwoko Harwoko, Werner E.G. Müller, Alexandra Hamacher, Matthias U. Kassack, Mostafa A. Fouad, Mohamed S. Kamel, Wenhan Lin, Weaam Ebrahim, Zhen Liu, Peter Proksch Bioorganic and Medicinal Chemistry, 2019
Inhibitory effect of ethanolic extract of Psidium guajava leaves in rat active cutaneus anaphylaxis reaction International Journal of Pharmaceutical and Clinical Research, 2016
Screening of volatile compounds of Brotowali (Tinospora crispa) and antifungal activity against Candida albicans International Journal of Pharmacognosy and Phytochemical Research, 2015
Total flavonoid content and in vivo hypotensive effect of chloroform insoluble fraction of Centella asiatica leaf extract International Food Research Journal, 2015
Triterpenoid-rich fraction of centella asiatica leaves and in vivo antihypertensive activity International Food Research Journal, 2014
RECENT SCHOLAR PUBLICATIONS
Optimization of Secondary Metabolite Production in the Sponge Associated Bacterium Roseivirga sp. PAP. 19 Using the OSMAC Approach AS Pradani, H Harwoko, MDN Meinita, R Riviani, U Mettal Jurnal Ilmiah Perikanan dan Kelautan 18 (1), 272 , 2026 2026
Antimalarial Activity Screening from Endophytic Fungus of Red Ginger (Zingiber officinale): in vitro and in silico Studies MA Artasasta, DM Fardilla, H Rasyid, D Listyorini, WE Putra, D Handayani Sains Malaysiana 54 (6), 1523-1534 , 2025 2025 Citations: 3
Molecular Networking Analysis and Antibacterial Potential of Ethyl Acetate Extracts of Sinomicrobium sp. PAP. 21 using OSMAC Method H Harwoko, AT Rahmawati, R Riyanti Majalah Obat Tradisional 29 (2), 213-223 , 2024 2024 Citations: 3
Draft Genome Sequence of Sinomicrobium sp. Strain PAP.21, Isolated from a Coast Sample of Papua, Indonesia Riyanti, CM Zumkeller, M Spohn, S Mihajlovic, O Schwengers, ... Microbiology Resource Announcements 12 (4), e01268-22 , 2023 2023
Draft Genome Sequences of Algoriphagus sp. Strain PAP.12 and Roseivirga sp. Strain PAP.19, Isolated from Marine Samples from Papua, Indonesia Riyanti, CM Zumkeller, M Spohn, S Mihajlovic, O Schwengers, ... Microbiology Resource Announcements 12 (4), e01264-22 , 2023 2023 Citations: 2
Formulation and evaluation of “Nata de Curcuma” AA Rahmah, A Putri, F Ivena, M Ramadhanti, H Harwoko AIP Conference Proceedings 2586 (1), 030001 , 2023 2023
EFEK ANTIHIPERURISEMIA FRAKSI KLOROFORM DAN FRAKSI ETIL ASETAT BATANG BROTOWALI (Tinospora crispa) PADA MODEL MENCIT HIPERURISEMIA H Harwoko, ED Utami, W Warsinah Jurnal Tumbuhan Obat Indonesia 15 (2), 126-135 , 2022 2022
PENINGKATAN PENGETAHUAN KADER PEMBERDAYAAN KESEJAHTERAAN KELUARGA DALAM PEMANFAATAN TANAMAN OBAT KELUARGA H Harwoko, HN Baroroh, ED Utami, NA Choironi, MS Fareza Prosiding Seminar Nasional LPPM Unsoed 11 (1) , 2022 2022
Antimicrobial compound from Trichoderma harzianum, an endophytic fungus associated with ginger (Zingiber officinale) H Harwoko, J Lee, G Daletos, M Feldbrügge, R Kalscheuer, P Proksch JKKI: Jurnal Kedokteran dan Kesehatan Indonesia, 151-157 , 2021 2021 Citations: 4
Metabolite Diversity of Endophytic Fungi Associated with Medicinal Plants H Harwoko https://docserv.uni-duesseldorf.de/servlets/DerivateServlet/Derivate-56325 … , 2021 2021
Dithiodiketopiperazine derivatives from endophytic fungi Trichoderma harzianum and Epicoccum nigrum H Harwoko, G Daletos, F Stuhldreier, J Lee, S Wesselborg, M Feldbrügge, ... Natural Product Research 35 (2), 257-265 , 2021 2021 Citations: 103
Jurnal Kedokteran dan Kesehatan Indonesia H Harwoko, J Lee, G Daletos, M Feldbrügge, R Kalscheuer, P Proksch 2021
Efek Hipoglikemik Larva Undur-Undur Darat (Myrmeleon frontalis) dari Tes Toleransi Glukosa Oral Pada Tikus H Harwoko, RP Aryani, S Novita Majalah Farmasi dan Farmakologi (2), 63-65 , 2021 2021 Citations: 2
Azacoccones FH, new flavipin-derived alkaloids from an endophytic fungus Epicoccum nigrum MK214079 H Harwoko, J Lee, R Hartmann, A Mándi, T Kurtán, WEG Müller, ... Fitoterapia 146, 104698 , 2020 2020 Citations: 22
Phytochemical analysis and antioxidant activity of Brotowali (Tinospora crispa L. Mier) stem W Warsinah, HN Baroroh, H Harwoko Molekul 15 (2), 73-78 , 2020 2020 Citations: 21
Phytochemical analysis and evaluation of purified extract of Tinospora crispa stem for in vivo antihyperuricemic effect H Harwoko, W Warsinah Journal of Reports in Pharmaceutical Sciences 9 (1), 46-51 , 2020 2020 Citations: 15
Anticancer Activity of Zingiber officinale and Piper retrofractum Extract Combination on Hela Cell Line H Ekowati, S Septiyaningsih, H Harwoko, T Trisnowati Indonesian Journal of Plant Medicine 3 (2), 142065 , 2019 2019
Biotransformation of Host Plant Flavonoids by the Fungal Endophyte Epicoccum nigrum H Harwoko, R Hartmann, G Daletos, E Ancheeva, M Frank, Z Liu, ... ChemistrySelect 4 (45), 13054-13057 , 2019 2019 Citations: 16
Cyclic heptapeptides from the soil-derived fungus Clonostachys rosea NM Abdel-Wahab, H Harwoko, WEG Müller, A Hamacher, MU Kassack, ... Bioorganic & Medicinal Chemistry 27 (17), 3954-3959 , 2019 2019 Citations: 25
Penapisan Senyawa Antikanker Dari Batang Brotowali (Tinospora crispa L) Dan Uji Aktivitas Sitotoksik Pada Kultur Sel Widr W Warsinah, H Harwoko Acta Pharmaciae Indonesia: Acta Pharm Indo 6 (2), 51-59 , 2018 2018
MOST CITED SCHOLAR PUBLICATIONS
Dithiodiketopiperazine derivatives from endophytic fungi Trichoderma harzianum and Epicoccum nigrum H Harwoko, G Daletos, F Stuhldreier, J Lee, S Wesselborg, M Feldbrügge, ... Natural Product Research 35 (2), 257-265 , 2021 2021 Citations: 103
Formulasi Sediaan Krim Antioksidan Ekstrak Etanolik Daun Alpukat (Persea americana Mill.) D Mailana, Nuryanti, Harwoko Acta Pharm. Indones 4, 21-28 , 2016 2016 Citations: 72
Triterpenoid-rich fraction of Centella asiatica leaves and in vivo antihypertensive activity Harwoko, S Pramono, AE Nugroho International Food Research Journal 21 (1), 149 , 2014 2014 Citations: 46
Cyclic heptapeptides from the soil-derived fungus Clonostachys rosea NM Abdel-Wahab, H Harwoko, WEG Müller, A Hamacher, MU Kassack, ... Bioorganic & Medicinal Chemistry 27 (17), 3954-3959 , 2019 2019 Citations: 25
Azacoccones FH, new flavipin-derived alkaloids from an endophytic fungus Epicoccum nigrum MK214079 H Harwoko, J Lee, R Hartmann, A Mándi, T Kurtán, WEG Müller, ... Fitoterapia 146, 104698 , 2020 2020 Citations: 22
Phytochemical analysis and antioxidant activity of Brotowali (Tinospora crispa L. Mier) stem W Warsinah, HN Baroroh, H Harwoko Molekul 15 (2), 73-78 , 2020 2020 Citations: 21
Screening of volatile compounds of Brotowali (Tinospora crispa) and antifungal activity against Candida albicans Warsinah, Harwoko, Nuryanti International J Pharmacogn Phytochem Res 7 (1), 132-136 , 2015 2015 Citations: 18
Biotransformation of Host Plant Flavonoids by the Fungal Endophyte Epicoccum nigrum H Harwoko, R Hartmann, G Daletos, E Ancheeva, M Frank, Z Liu, ... ChemistrySelect 4 (45), 13054-13057 , 2019 2019 Citations: 16
Phytochemical analysis and evaluation of purified extract of Tinospora crispa stem for in vivo antihyperuricemic effect H Harwoko, W Warsinah Journal of Reports in Pharmaceutical Sciences 9 (1), 46-51 , 2020 2020 Citations: 15
Quality standardization of brotowali (Tinospora crispa) stem extract H Harwoko, NA Choironi Majalah Obat Tradisional 21 (1), 6-11 , 2016 2016 Citations: 14
Total flavonoid content and in vivo hypotensive effect of chloroform insoluble fraction of Centella asiatica leaf extract E Nansy, Harwoko, S Pramono, AE Nugroho International Food Research Journal 22 (5), 2119 , 2015 2015 Citations: 13
An extract of Zingiber officinale and Piper retrofractum combination and its effect to cancer cell line H Ekowati, S Septiyaningsih, H Harwoko Indonesian Journal of Cancer Chemoprevention 2 (1), 173-181 , 2011 2011 Citations: 11
Formulasi dan Evaluasi Suppositoria Ekstrak Terpurifikasi Daun Lidah Buaya (Aloe vera) N Nuryanti, H Harwoko, RS Jeanita, AR Azhar Acta Pharmaciae Indonesia 4 (1), 37-44 , 2016 2016 Citations: 7
Antibacterial activity of two isolated endophytic fungi extracts associated with Indonesian mangrove plant Rhizophora mucronata MS Fareza, NA Choironi, H Harwoko, S Sunarto Pharmaciana 8 (1), 169-175 , 2018 2018 Citations: 6
Aktivitas Sitotoksik Fraksi n-Heksana: Kloroform dari ekstrak methanol kulit batang mangrove (Rhizopora mucronata) pada sel kanker myeloma Harwoko, ED Utami Majalah Obat Tradisional 15 (2), 51-55 , 2010 2010 Citations: 6
Antimicrobial compound from Trichoderma harzianum, an endophytic fungus associated with ginger (Zingiber officinale) H Harwoko, J Lee, G Daletos, M Feldbrügge, R Kalscheuer, P Proksch JKKI: Jurnal Kedokteran dan Kesehatan Indonesia, 151-157 , 2021 2021 Citations: 4
Inhibitory Effect of Ethanolic Extract of Psidium guajava Leaves in Rat Active Cutaneus Anaphylaxis Reaction HN Baroroh, ED Utami, Harwoko International Journal of Pharmacology and Clinical Research (IJPCR) 8 (1), 1-5 , 2016 2016 Citations: 4
Antimalarial Activity Screening from Endophytic Fungus of Red Ginger (Zingiber officinale): in vitro and in silico Studies MA Artasasta, DM Fardilla, H Rasyid, D Listyorini, WE Putra, D Handayani Sains Malaysiana 54 (6), 1523-1534 , 2025 2025 Citations: 3
Molecular Networking Analysis and Antibacterial Potential of Ethyl Acetate Extracts of Sinomicrobium sp. PAP. 21 using OSMAC Method H Harwoko, AT Rahmawati, R Riyanti Majalah Obat Tradisional 29 (2), 213-223 , 2024 2024 Citations: 3
Draft Genome Sequences of Algoriphagus sp. Strain PAP.12 and Roseivirga sp. Strain PAP.19, Isolated from Marine Samples from Papua, Indonesia Riyanti, CM Zumkeller, M Spohn, S Mihajlovic, O Schwengers, ... Microbiology Resource Announcements 12 (4), e01264-22 , 2023 2023 Citations: 2
