C C S Vasundhara

EDUCATION

MSc., MPhil., .,

RESEARCH INTERESTS

Diabetes and Cardiovascular diseases

Scopus Publications

Cytoprotective and Antioxidant Effects of Silver Nanoparticles Synthesised from the Pisonia grandis L. Extracts Against Hydrogen Peroxide-induced Toxicity in Human Cardiomyocytes
G. O. Veena, S. Gayathri Devi, Shubashini K. Sripathi, C. C. S. Vasundhara
Journal of Natural Remedies, 2026
Background: Myocardial Infarction (MI) is a major cause of global mortality, with oxidative stress being a central mechanism underlying cardiomyocyte injury. Hydrogen peroxide (H₂O₂) induced oxidative damage is widely used as an in vitro model to mimic myocardial stress. Plant-mediated nanomaterials have emerged as promising cardioprotective agents due to their antioxidant potential. Aim: This study evaluates the protective effect of Polyvinyl Alcohol (PVA)-encapsulated silver nanoparticles synthesised using the ethanolic extract of Pisonia grandis leaves (PVA AgNPs Pg) against H₂O₂-induced oxidative stress in Human Cardiomyocytes (HCM). Methods: The synthesised nanoparticles were characterised using Ultraviolet (UV)–visible spectroscopy, Transmission Electron Microscopy (TEM), X-ray Powder Diffraction (XRD), Fourier Transform Infrared (FTIR) spectroscopy, Energy-Dispersive X-ray (EDX) analysis, and Scanning Electron Microscopy (SEM). HCM cells were pretreated with PVA AgNPs Pg followed by H₂O₂ exposure. Cell viability, intracellular Reactive Oxygen Species (ROS), antioxidant enzyme levels (SOD, CAT, GSH, and GPX), and mitochondrial integrity using Mitotracker Green (MTG) staining were evaluated. Digoxin was used as the reference drug. Results: H₂O₂ exposure significantly decreased cell viability and induced morphological alterations. Pretreatment with PVA AgNPs Pg markedly improved cell survival and significantly enhanced antioxidant enzyme levels. ROS generation was effectively suppressed, demonstrating protection comparable to digoxin. Confocal microscopy revealed modulation of mitochondrial function, indicated by altered MTG expression in treated cells. Conclusion: PVA-encapsulated silver nanoparticles derived from P. grandis exert significant cardioprotective effects against oxidative stress by enhancing antioxidant defence, reducing ROS, and preserving mitochondrial integrity, suggesting their potential in myocardial infarction management. Major Findings: PVA-encapsulated silver nanoparticles from P. grandis leaves showed cardioprotective effects against H₂O₂-induced oxidative stress by enhancing antioxidant enzymes, reducing ROS generation, preserving mitochondrial function, and demonstrating efficacy comparable to digoxin.
Exploring the Therapeutic Potential: Investigating the Synthesis, Characterization and in vitro Anti-Diabetic Efficacy of Silver Nanoparticle Ethanolic Extract from Boerhavia diffusa
A. Reena Joy, S. Gayathri Devi, Slade Samuel, C.C.S. Vasundhara, M.S. Priyadharshini
Research Journal of Biotechnology, 2025
Many studies explore the effects of Boerhavia diffusa (Bd) combined with synthesized silver-nanoparticles extract (AgNPs) for treating a variety of conditions, although rarely for diabetes. To address this, we investigate the inhibitory effect of BdAgNPs in standard in vitro antidiabetic assays using the whole plant extract dissolved in ethanol (EBdAgNPs). EBdAgNPs was characterized by UV-Visible, XRD and FT-IR spectroscopy techniques. The assays consisted of α-amylase, α-glucosidase, protein glycation, non-enzymatic glycosylation of hemoglobin, glucose uptake by yeast and glucose diffusion. The formation of AgNPs was revealed by observing a surface Plasmon resonance peak at 410 nm. Notably, EBdAgNPs exhibited robust inhibitory effects in comparison to standard control drugs within the assays. These compelling findings strongly indicate the potential of EBdAgNPs as a viable avenue for treating diabetes mellitus. Further clinical investigations are warranted to fully explore EBdAgNPs potential in novel therapeutic approaches for managing diabetes and related conditions.
In vitro anti diabetic activity of PVA encapsulated silver nanoparticles of ethanolic extract of Pisonia grandis leaves
G Amrutha Veena, Devi S. Gayathri, K. Sripathi Shubashini, C.C.S. Vasundhara
Research Journal of Biotechnology, 2025
Diabetes mellitus is a chronic metabolic ailment that progresses beyond a person's lifetime. It is brought on by a relative shortage of insulin synthesis, leading to variable degrees of insulin resistance. The present study involves biosynthesis of poly vinyl alcohol (PVA) encapsulated silver nanoparticles of ethanolic extract of Pisonia grandis leaves (PVA AgNPs Pg) along with the evaluation of their potential biomedical applications. Analytical techniques like UV spectroscopy, XRD, SEM, EDX, TEM, FTIR and Zeta potential measurement were employed to assess the separated silver nanoparticles. PVA AgNPs Pg was also used to investigate the anti-diabetic effect in vitro. These studies have showed significant efficacy in inhibiting the enzymes DPPIV, alpha-amylase and alpha-glucosidase. Therefore, it is reasonable to conclude that silver nanoparticles fabricated by green synthesis might be used as a phytomedicine to treat diabetes.
Antihyperlipidemic property of Boerhavia diffusa leaf extract in streptozotocin-induced diabetic rats
Vasundhara Ccs, Gayathri Devi S
Asian Journal of Pharmaceutical and Clinical Research, 2018
Objectives: The aim of the present research was to evaluate the antidiabetic, hyperlipidemic, and histopathological analysis in streptozotocin-induced diabetic rats (60 mg/kg body weight) using the ethanolic extract of leaves of Boerhavia diffusa (ELBD) (500 mg/kg body weight).Method: The rats were orally administered with the leaf extract for 45 days. Fasting blood was collected at the end of the experimental period by cardiac puncture to carry out the biochemical parameters, the organs such as liver, kidney, and pancreas were also excised to perform the histopathological analysis by fixing in 10% formalin solution.Results: Oral administration with the leaf extract resulted in decrease in the levels of blood glucose, with a concomitant increase in their body weight. The extracts also produced a significant decrease in the lipid levels when compared with the diabetic groups. Moreover, the extracts also exerted a favorable effect on the histopathological changes of liver, pancreas, and kidney.Conclusion: The results of the present study revealed that the ELBD possess antidiabetic and antihyperlipidemic properties. These effects may be due to the presence of bioactive components justifying its ethnomedical use.
Evaluation of antioxidant potential in the leaves and seeds of Boerhavia diffusa
International Journal of Pharmaceutical Sciences Review and Research, 2015
Free radical scavenging activity of leaves of Boerhavia diffusa
International Journal of Pharma and Bio Sciences, 2015