Pharmacology, Toxicology and Pharmaceutics, Multidisciplinary
5
Scopus Publications
127
Scholar Citations
6
Scholar h-index
5
Scholar i10-index
Scopus Publications
Integrated In Silico and In Vivo Analysis of Vitamin D3 Supplementation in Obesity and Diabetes Alfreds Roosevelt, Rina Agustina, Filmaharani Filmaharani, Anggun Nurhidayah, Muhammad Rayza Azmin, Jumriani Jumriani, Ratnawati Ratnawati, Muh Arfandy Gunawan, Nadila Pratiwi Latada, Mukarram Mudjahid, Widya Hardiyanti, Asbah Asbah, Firzan Nainu Trends in Sciences, 2026 Obesity and diabetes are interconnected metabolic disorders with a rising prevalence worldwide. Although vitamin D3 is known to regulate glucose metabolism and oxidative stress, its ability to inhibit digestive enzymes linked to adipogenesis has not been demonstrated. This study proposed that vitamin D3 possesses dual anti-obesity and antidiabetic activities by targeting pancreatic lipase, α-amylase, and α-glucosidase, and confirmed this hypothesis through in silico docking and in vivo experiments using Drosophila melanogaster. Docking simulations using AutoDock Vina showed that vitamin D3 had a stronger affinity for pancreatic lipase (−7.5 kcal/mol) than orlistat (−6.8 kcal/mol), with stable hydrophobic interactions at the catalytic sites. The D. melanogaster w1118 strain was reared on a high-fat diet (2% virgin coconut oil) supplemented with vitamin D3 (10 or 100 mM). The experimental groups (n = 20 flies per group, 5 replicates) were assessed for hemolymph glucose, locomotor activity, oxidative stress (NBT assay), and gene expression <trbl and sod1>. Data were analyzed using 1-way ANOVA with Tukey’s post-hoc test (p < 0.05). Vitamin D3 supplementation significantly reduced hemolymph glucose and ROS levels, improved crawling performance, and restored trbl and sod1 expression in HFD-fed flies. These effects were consistent with docking predictions, indicating that enzyme inhibition may underlie the observed metabolic benefits. In conclusion, vitamin D3 shows promising antiobesity and antidiabetic effects in a D. melanogaster model, supporting its role as a potential modulatory supplement. However, limitations such as the use of an invertebrate model, high compound concentrations, and the absence of mammalian or clinical validation highlight the need for further studies in higher organisms. HIGHLIGHTS Vitamin D3 exhibits strong in silico binding affinity to lipase and α-amylase. In vivo exposure to Vitamin D3 reduces cholesterol and glucose levels in Drosophila larvae Vitamin D3 promotes larval growth and locomotor activity under high-fat diet conditions Vitamin D3 upregulates the expression of trbl, sod1, and cat genes Vitamin D3 shows potential as a low-cost adjunctive therapy for obesity management GRAPHICAL ABSTRACT
In Vivo and In Silico Analysis of Quercetin’s Effects on Glycemic Regulation Jumriani Jumriani, Muhammad Aswad, Ratnawati Ratnawati, Filmaharani Filmaharani, Anggun Nurhidayah, Muhammad Rayza Azmin, Alfreds Roosevelt, Rizky Alfiana, Widya Hardiyanti, Nadila Pratiwi Latada, Mukarram Mudjahid, Firzan Nainu Scientifica, 2026 Background & Objective Diabetes mellitus is a global health issue caused by chronic hyperglycemia. Although various therapeutic options are available, each carries potential side effects, prompting growing interest in exploring natural compounds as alternative treatments. Quercetin, a flavonoid known for its antioxidant and anti‐inflammatory properties, is suspected to play a role in glucose regulation, although its molecular mechanisms remain incompletely understood. This study aimed to analyze the in vivo effects of quercetin on the phenotype of Drosophila melanogaster and to validate its potential mechanism through an in silico molecular docking approach, focusing on its interaction with diabetes‐related enzyme targets. Methods Phenotypic evaluation included measurements of body morphology, locomotor activity, survival rate, and hemolymph glucose levels. Molecular analyses were conducted using reverse transcription quantitative PCR (RT‐qPCR), while molecular docking studies were performed to assess quercetin’s interaction with the enzyme dipeptidyl peptidase‐4 (DPP4). Results Quercetin significantly reduced hemolymph glucose levels in both larvae ( p < 0.0001) and adult flies ( p < 0.001) within the concentration range of 1–10 μm without affecting adult flies’ locomotor activity or survival. Additionally, quercetin enhanced the expression of genes involved in metabolic and stress response and improved growth parameters and motor activity in larvae subjected to a high‐sugar diet. Molecular docking studies revealed that quercetin has a high affinity for DPP4, supporting its proposed hypoglycemic mechanism. Conclusion This study provides both phenotypic and molecular evidence that quercetin exerts hypoglycemic effects in D. melanogaster , potentially mediated through DPP4 inhibition and modulation of metabolic and stress‐response pathways. These findings offer new insight into the mechanisms of quercetin in glucose regulation.
Hyperglycemia-Induced Apoptosis and Phagocytosis Suppression in Drosophila Anggun Nurhidayah, Habibie Habibie, Filmaharani Filmaharani, Alfreds Roosevelt, Muhammad Rayza Azmin, Ratnawati Ratnawati, Jumriani Jumriani, Widya Hardiyanti, Asbah Asbah, Nadila Pratiwi Latada, Mukarram Mudjahid, Firzan Nainu Trends in Sciences, 2026 Hyperglycemia, characterized by elevated blood glucose levels, is a major risk factor for the development of type 2 diabetes mellitus and its associated complications, including organ dysfunction and immune system impairment. Oxidative stress induced by hyperglycemia is known to disrupt cellular immune mechanisms, including phagocytosis, which is crucial for eliminating pathogens and damaged cells. This study aimed to assess the effects of a high-sugar diet (HSD) on phenotypic traits, phagocytosis, and expression of immune-related genes in Drosophila melanogaster wild-type w1118 and mutant itgbn2, which serves as a model organism for hyperglycemia. A phenotypic assay was conducted by observing glucose levels, development, body weight, crawling, and activity, as well as molecular analysis using the RT-qPCR method. The results showed that HSD treatment significantly increased hemolymph glucose levels and reduced larvae body weight, crawling ability, and developmental progression to the pupae and adult stages. Molecular analysis revealed a significant upregulation of pro-apoptotic gene expression in w1118 larvae, rpr expression increased by approximately 9% (p < 0.05), while in the itgbn² mutant, the increase was more substantial, reaching about 21% (p < 0.01). The grim expression was also significantly elevated in itgbn² larvae by around 25% (p < 0.001). In contrast, the phagocytic receptor gene drpr was markedly downregulated in w1118 larvae, showing a 50% reduction under HSD (p < 0.0001). In contrast, no significant changes were observed in the itgbn² mutant, a phagocytosis receptor involved in the elimination of apoptotic cells, which may reflect limited activation of integrin associated signaling pathways necessary for transcriptional responsiveness under hyperglycemic stress. Overall, these findings suggest that hyperglycemia induced by an HSD is associated with increased apoptosis and reduced phagocytic activity, possibly through disrupted integrin-mediated signaling in the itgbn² mutant, which may contribute to cellular immune dysfunction under hyperglycemic conditions. This study offers new insights into the molecular mechanisms underlying immune dysregulation in hyperglycemic conditions, highlighting the therapeutic potential of targeting phagocytic pathways to prevent diabetes-related complications in the future. HIGHLIGHTS A high-sugar diet triggers hyperglycemia-like symptoms in Drosophila Immune disruption was evident through reduced phagocytosis and increased apoptotic activity. The phagocytic receptor integrin βν plays an important role in glucose homeostasis. Integrin βν dysfunction leads to impaired cellular immunity under hyperglycemic conditions. These findings offer novel insights into the link between apoptosis, phagocytosis, and metabolic stress. GRAPHICAL ABSTRACT
Aspirin-Mediated Reduction of Glucose Level and Inflammation in Drosophila melanogaster Muhammad Rayza Azmin, Habibie Habibie, Filmaharani Filmaharani, Alfreds Roosevelt, Anggun Nurhidayah, Muhammad Rasul Pratama, Widya Hardiyanti, Nadila Pratiwi Latada, Mukarram Mudjahid, Dewi Yuliana, Firzan Nainu ACS Omega, 2025 Diabetes mellitus (DM), particularly type 2 diabetes mellitus (T2DM), is a global health challenge marked by chronic hyperglycemia and inflammation, which contributes to both metabolic dysregulation and associated complications. Inflammation exacerbates T2DM by activating immune signaling pathways and promoting insulin resistance. This study aims to investigate the interplay between hyperglycemia and inflammation and to explore the therapeutic potential of aspirin in mitigating these processes using Drosophila melanogaster as a model organism. We utilized the PGRP-LBΔ strain, which exhibits dysregulated immune responses due to the loss of the PGRP-LB gene, leading to a phenotype resembling human autoinflammatory conditions. Larvae of the PGRP-LBΔ were fed a high-sucrose diet to induce increased glucose levels, mimicking the metabolic disturbances of T2DM. Aspirin, at different concentrations, was administered to assess its effects on high glucose level-induced inflammation. The results demonstrated that aspirin significantly improved hemolymph glucose levels, larval size, weight, and development. Additionally, aspirin enhanced larval mobility and reduced glucose level-associated immune dysfunction, as evidenced by changes in the expression of key immune and insulin-related genes. These findings highlight the utility of D. melanogaster as an effective and cost-efficient model to investigate the molecular mechanisms of T2DM and inflammation. The study also provides preliminary evidence for the potential of aspirin as an anti-inflammatory agent to modulate glucose levels and inflammation in T2DM, offering a promising avenue for therapeutic development.
In Silico and In Vivo Investigation of the Anti-Hyperglycemic Effects of Caffeic Acid Ratnawati Ratnawati, Muhammad Aswad, Jumriani Jumriani, Anggun Nurhidayah, Muhammad Rayza Azmin, Filmaharani Filmaharani, Alfreds Roosevelt, Widya Hardiyanti, Nadila Pratiwi Latada, Mukarram Mudjahid, Firzan Nainu ACS Omega, 2025 Hyperglycemia, characterized by elevated blood glucose levels, is a major risk factor for diabetes mellitus and its complications. While conventional therapies are effective, they are often associated with side effects and high costs, necessitating alternative strategies. This study evaluates the potential of caffeic acid (CA), a phenolic compound with reported antihyperglycemic properties, using both in silico and in vivo approaches. Molecular docking simulations revealed that CA demonstrates a strong binding affinity to protein tyrosine phosphatase 1B (PTP1B), a critical enzyme in glucose metabolism, with superior interaction profiles compared to the reference drug, ertiprotafib. In the in vivo studies, a Drosophila melanogaster model was used to investigate the effects of CA under hyperglycemic conditions induced by a high-sugar diet. Treatment with CA, particularly at a concentration of 500 μM, significantly reduced hemolymph glucose levels and improved several physiological and behavioral parameters, including survival rates, body size, body weight, and larval movement. Furthermore, gene expression analysis demonstrated that CA modulates key metabolic and stress-related pathways, enhancing glucose homeostasis and reducing metabolic stress. These findings highlight the dual utility of in silico and in vivo methodologies in elucidating the antihyperglycemic potential of CA. The results support the development of CA as a cost-effective and ethically viable therapeutic candidate with implications for diabetes management in resource-limited settings.
RECENT SCHOLAR PUBLICATIONS
In Vivo and In Silico Analysis of Quercetin’s Effects on Glycemic Regulation J Jumriani, M Aswad, R Ratnawati, F Filmaharani, A Nurhidayah, ... Scientifica 2026 (1), 5159975 , 2026 2026
Hyperglycemia-Induced Apoptosis and Phagocytosis Suppression in Drosophila A Nurhidayah, H Habibie, F Filmaharani, A Roosevelt, MR Azmin, ... Trends in Sciences 23 (5), 11170-11170 , 2026 2026
Integrated In Silico and In Vivo Analysis of Vitamin D3 Supplementation in Obesity and Diabetes A Roosevelt, R Agustina, F Filmaharani, A Nurhidayah, MR Azmin, ... Trends in Sciences 23 (4), 11600-11600 , 2026 2026 Citations: 1
Profile of Self Medication Practices in the Tarramatekkeng Village Luwu District South Sulawesi R Asrina, A Roosevelt, H Hasma, R Rusli Media Kesehatan Politeknik Kesehatan Makassar 20 (2), 421-426 , 2025 2025 Citations: 1
Evaluasi Penggunaan Antibiotik Pada Pasien Dewasa di Apotek N A Roosevelt, R Asrina, A masri Jurnal pendidikan tambusai 9 (2), 25763-25767 , 2025 2025
Aspirin-Mediated Reduction of Glucose Level and Inflammation in Drosophila melanogaster MR Azmin, H Habibie, F Filmaharani, A Roosevelt, A Nurhidayah, ... ACS Omega , 2025 2025 Citations: 6
In silico and in vivo investigation of the anti-hyperglycemic effects of caffeic acid R Ratnawati, M Aswad, J Jumriani, A Nurhidayah, MR Azmin, ... ACS omega 10 (14), 14052-14062 , 2025 2025 Citations: 7
EKSPLORASI EFEK FARMAKOLOGI VITAMIN D3 PADA KOMPLIKASI OBESITAS-DIABETES MENGGUNAKAN MODEL Drosophila melanogaster= EXPLORATION OF PHARMACOLOGICAL EFFECTS OF VITAMIN D3 ON … A ROOSEVELT Universitas Hasanuddin , 2025 2025
The Potential of Hyperglycemia Combination of Watermelon (Citrullus lanatus) Rind Extract and Red Dragon Fruit (Hylocereus polyrhizus) Peel Extract KS EDI, A ROOSEVELT, G PATANDUNG, A AMIRUDDIN JURNAL FARMASI SANDI KARSA Учредители: Jurusan Farmasi Politeknik Sandi … , 2024 2024
The Place to Purchase Drugs for Community Self-Medication at Green Hills Moncongloe Housing, Maros Regency R Asrina, A Roosevelt, A Julyiansyah Jurnal Farmasi Sandi Karsa 9 (1), 1-8 , 2023 2023
Overview of the Level of Knowledge of Narisa Pharmacy Consumers Against Cough Self-Medication H Herman, A Roosevelt, U Kasman Jurnal Farmasi Sandi Karsa 8 (2), 132-140 , 2022 2022 Citations: 2
Factors Causing Expired Medicine in Puskesmas Cendrawasih Makassar City MF Hasyim, A Roosevelt, I Enriana Jurnal Farmasi Sandi Karsa 8 (1), 56-72 , 2022 2022
PENYULUHAN PENGGUNAAN OBAT YANG BAIK DAN BENAR PADA IBU HAMIL R Rusli, M Megawati, Z Zulfiah, SE Kamal, R Asrina, SHA Lau, H Herman, ... Media Implementasi Riset Kesehatan 2 (2), 47-51 , 2021 2021
Aktivitas antioksidan pada kopi Arabika (Coffea arabica L.) yang diolah dengan mesin espresso dan manual brew pour over V60 R Asrina, Z Zulfiah, SE Kamal, A Roosevelt, G Patandung, M Murniati, ... Media Farmasi 17 (2), 204-210 , 2021 2021 Citations: 10
The Effect of God's Crown Fruit Extract and Cinnamon Extract On Decrease Total Cholesterol Levels In Rats White Male SE Kamal, Z Zulfiah, R Asrina, H Herman, G Patandung, A Roosevelt, ... Urban Health 3 (1) , 2021 2021
The Evaluation of the Utilization of Guava Leaf Plant as Medicine Traditional Antidiaries in Kelurahan Tamalanrea Jaya, Makassar City M Murniati, A Roosevelt, N Hayati JURNAL FARMASI SANDI KARSA Учредители: Jurusan Farmasi Politeknik Sandi … , 2021 2021
Inhibitory Test of Sweet Potato Leaves Infusion (Impomea batatas L.) Toward the Growth of (Propeniumbacterium acnes) Z Zulfiah, M Megawati, H Herman, SHA Lau, MF Hasyim, M Murniati, ... Jurnal Farmasi Sandi Karsa 7 (1), 46-51 , 2021 2021
Uji Identifikasi Senyawa Alkaloid Ekstrak Metanol Daun Kelor (Moringa oleifera Lamk) Menggunakan Metode Metode Kromatografi Lapis Tipis Z Zulfiah, H Herman, NI AR, Y Kadang, A Roosevelt, SHA Lau, M Murniati, ... Jurnal Farmasi Sandi Karsa 6 (2), 83-87 , 2020 2020 Citations: 2
Uji toksisitas ekstrak rimpang temu hitam (Curcuma aeruginosa Roxb.) terhadap larva udang (Artemia salina Leach) dengan metode brine shrimp lethality test (BSLT) Z Zulfiah, M Megawati, H Herman, SHA Lau, MF Hasyim, M Murniati, ... Jurnal Farmasi Sandi Karsa 6 (1), 44-49 , 2020 2020 Citations: 13
Uji Identifikasi Senyawa Alkaloid Ekstrak Metanol Daun Kelor (Moringa oleifera Lamk) Menggunakan Metode Metode Kromatografi Lapis Tipis H Herman, Z ZULFIAH, M MEGAWATI, MF HASYIM, M MURNIATI, ... Jurnal Farmasi Sandi Karsa 6 (2), 83-87 , 2020 2020 Citations: 1
MOST CITED SCHOLAR PUBLICATIONS
FOrmulasi dan uji stabilitas krim ekstrak methanol daun beluntas (Pluchea indica L.) dari kota Benteng Kabupaten Kepulauan Selayar Provinsi Sulawesi Selatan A Roosevelt, SHA Lau, H Syawal Jurnal farmasi sandi karsa 5 (1), 19-25 , 2019 2019.0 Citations: 41
Formulasi dan Uji Stabilitas Fisik Sediaan Gel Ekstrak Kulit Buah Rambutan (Nephelium lappaceum L.) Sebagai Obat Sariawan Menggunakan Variasi Konsentrasi Basis Carbopol M Megawati, AR Yacobus Jurnal Farmasi Sandi Karsa 5 (1), 05-10 , 2019 2019.0 Citations: 22
Formulasi Dan Uji Stabilitas Krim Ekstrak Methanol Daun Beluntas Pluchea Indica L.) Dari Kota Benteng Kabupaten Kepulauan Selayar Provinsi Sulawesi Selatan AR Yacobus, SHA Lau, H Syawal Jurnal Farmasi Sandi Karsa 5 (1), 19-25 , 2019 2019.0 Citations: 14
Uji toksisitas ekstrak rimpang temu hitam (Curcuma aeruginosa Roxb.) terhadap larva udang (Artemia salina Leach) dengan metode brine shrimp lethality test (BSLT) Z Zulfiah, M Megawati, H Herman, SHA Lau, MF Hasyim, M Murniati, ... Jurnal Farmasi Sandi Karsa 6 (1), 44-49 , 2020 2020.0 Citations: 13
Aktivitas antioksidan pada kopi Arabika (Coffea arabica L.) yang diolah dengan mesin espresso dan manual brew pour over V60 R Asrina, Z Zulfiah, SE Kamal, A Roosevelt, G Patandung, M Murniati, ... Media Farmasi 17 (2), 204-210 , 2021 2021.0 Citations: 10
In silico and in vivo investigation of the anti-hyperglycemic effects of caffeic acid R Ratnawati, M Aswad, J Jumriani, A Nurhidayah, MR Azmin, ... ACS omega 10 (14), 14052-14062 , 2025 2025.0 Citations: 7
Aspirin-Mediated Reduction of Glucose Level and Inflammation in Drosophila melanogaster MR Azmin, H Habibie, F Filmaharani, A Roosevelt, A Nurhidayah, ... ACS Omega , 2025 2025.0 Citations: 6
Identifikasi Senyawa Kimia Daun Bidara (Ziziphusmauritiana Lam) Dari Kabupaten Timor Tengah Selatan Provinsi NTT Secara Kromatografi Lapis Tipis Dan Kromatografi Kolom A Roosevelt, A Ghari Jurnal Farmasi Sandi Karsa 4 (7), 1-6 , 2018 2018.0 Citations: 5
Overview of the Level of Knowledge of Narisa Pharmacy Consumers Against Cough Self-Medication H Herman, A Roosevelt, U Kasman Jurnal Farmasi Sandi Karsa 8 (2), 132-140 , 2022 2022.0 Citations: 2
Uji Identifikasi Senyawa Alkaloid Ekstrak Metanol Daun Kelor (Moringa oleifera Lamk) Menggunakan Metode Metode Kromatografi Lapis Tipis Z Zulfiah, H Herman, NI AR, Y Kadang, A Roosevelt, SHA Lau, M Murniati, ... Jurnal Farmasi Sandi Karsa 6 (2), 83-87 , 2020 2020.0 Citations: 2
LOA, 2020. Formulasi dan Uji Stabilitas Fisik Sediaan Farmasi Rimpang Kunyit (Nephelium lappaceum l.) Sebagai Obat Sariawan Menggunakan Variasi Konsentrasi Basis Carbopol AR Megawati J. Farm. Sandi Karsa 5 (1) , 0 Citations: 2
Integrated In Silico and In Vivo Analysis of Vitamin D3 Supplementation in Obesity and Diabetes A Roosevelt, R Agustina, F Filmaharani, A Nurhidayah, MR Azmin, ... Trends in Sciences 23 (4), 11600-11600 , 2026 2026.0 Citations: 1
Profile of Self Medication Practices in the Tarramatekkeng Village Luwu District South Sulawesi R Asrina, A Roosevelt, H Hasma, R Rusli Media Kesehatan Politeknik Kesehatan Makassar 20 (2), 421-426 , 2025 2025.0 Citations: 1
Uji Identifikasi Senyawa Alkaloid Ekstrak Metanol Daun Kelor (Moringa oleifera Lamk) Menggunakan Metode Metode Kromatografi Lapis Tipis H Herman, Z ZULFIAH, M MEGAWATI, MF HASYIM, M MURNIATI, ... Jurnal Farmasi Sandi Karsa 6 (2), 83-87 , 2020 2020.0 Citations: 1
In Vivo and In Silico Analysis of Quercetin’s Effects on Glycemic Regulation J Jumriani, M Aswad, R Ratnawati, F Filmaharani, A Nurhidayah, ... Scientifica 2026 (1), 5159975 , 2026 2026.0
Hyperglycemia-Induced Apoptosis and Phagocytosis Suppression in Drosophila A Nurhidayah, H Habibie, F Filmaharani, A Roosevelt, MR Azmin, ... Trends in Sciences 23 (5), 11170-11170 , 2026 2026.0
Evaluasi Penggunaan Antibiotik Pada Pasien Dewasa di Apotek N A Roosevelt, R Asrina, A masri Jurnal pendidikan tambusai 9 (2), 25763-25767 , 2025 2025.0
EKSPLORASI EFEK FARMAKOLOGI VITAMIN D3 PADA KOMPLIKASI OBESITAS-DIABETES MENGGUNAKAN MODEL Drosophila melanogaster= EXPLORATION OF PHARMACOLOGICAL EFFECTS OF VITAMIN D3 ON … A ROOSEVELT Universitas Hasanuddin , 2025 2025.0
The Potential of Hyperglycemia Combination of Watermelon (Citrullus lanatus) Rind Extract and Red Dragon Fruit (Hylocereus polyrhizus) Peel Extract KS EDI, A ROOSEVELT, G PATANDUNG, A AMIRUDDIN JURNAL FARMASI SANDI KARSA Учредители: Jurusan Farmasi Politeknik Sandi … , 2024 2024.0
The Place to Purchase Drugs for Community Self-Medication at Green Hills Moncongloe Housing, Maros Regency R Asrina, A Roosevelt, A Julyiansyah Jurnal Farmasi Sandi Karsa 9 (1), 1-8 , 2023 2023.0
