Shashikant C Dhawale

Scopus Publications

Oral solid self-emulsifying system containing non-oncology drug combination for repurposing in melanoma treatment: In vitro cytotoxicity, and in vivo hematotoxicity and pharmacokinetic study
Rameshwar Ardad, Shashikant Dhawale, Arehalli Manjappa, Sunil T. Galatage, Ahmad Salawi
Journal of Research in Pharmacy, 2025
The goal of the current research was to identify a safe and effective non-oncology drug combination as a substitute to existing toxic chemotherapeutics for the treatment melanoma. Further intend was to develop an oral solid self-emulsifying drug delivery system (S-SEDDS) for concurrent delivery of identified combination. The drug containing S-SEDDS was prepared and characterized for flowability, compressibility, drug content, particle size and zeta potential, and in vitro cytotoxicity against melanoma cells. In silico molecular docking for drug with excipients interaction shows compatibility with each other. Moreover, the formulations were characterized in vivo for hepatotoxicity and pharmacokinetic study in rats. The S-SEDDS showed good flowability and compressibility. The particle size of S-SEDDS upon dilution was found in nanometer range. Furthermore, the in vitro cytotoxicity of S-SEDDS containing non-oncology drug (NOD) combination [ketoconazole (KCZ), tadalafil (TLF), disulfiram (DSR)] and docetaxel (DTX) was observed to be higher than S-SEDDS containing only NOD combination against mouse melanoma cells. No significant change in the hematological parameters, animal vital organs weights, and body weights were observed after oral administration of S SEDDS containing non-oncology drug combination with DTX indicating their safety. In addition, significant (p< 0.05) improvement in the oral bioavailability of DTX was observed following its administration in the form of S-SEDDS when compared to the Taxotere in the rats. The developed S-SEDDS containing non-oncology drug combination alone and in combination with docetaxel could be a promising and safe approach in the effective treatment of melanoma.
Preparation and Characterization of Quercetin Phytosome
School of Pharmacy, Swami Ramanand Teerth Marathwada University, Nanded-431606, India, S D Pande, S C Dhawale, School of Pharmacy, Swami Ramanand Teerth Marathwada University, Nanded-431606, India
International Journal of Drug Delivery Technology, 2025
The goal of this work is to improve quercetin's solubility and bioavailability by creating and characterizing quercetinphytosomes using the solvent evaporation technique. After obtaining phospholipids from ghee leftovers a quercetin-phosphatidylcholine complex was synthesized and its physicochemical characteristics were assessed. Analytical tools suchas Differential Scanning Calorimetry (DSC), Fourier Transform Infrared Spectroscopy (IR), X-ray Diffraction (XRD),Scanning Electron Microscopy (SEM), and Transmission Electron Microscopy (TEM) were used to conduct thecharacterization. Quercetin and phosphatidylcholine have formed a stable combination, as shown by DSC and IR studies.XRD, on the other hand, showed a change to a less crystalline state, suggesting improved solubility. SEM and TEM imagesshowed uniform, nanoscale spherical particles, supporting the successful encapsulation of quercetin. Thus, the 1:2 ratio isidentified as the optimal formulation for maximizing practical yield, underscoring the importance of phospholipidconcentration in achieving higher efficiency. The results indicate that the phytosome formulation enhances the stability,solubility, and bioavailability of quercetin, providing a promising strategy for its therapeutic applications
Citric acid crosslinked hydroxyethyl tamarind gum-based hydrogel films: A promising biomaterial for drug delivery
Vishwajeet Sampatrao Ghorpade, Kailas Krishnat Mali, Remeth Jacky Dias, Shashikant Chhaburao Dhawale, Rohit Ramesh Digole, et al.
International Journal of Biological Macromolecules, 2024
This investigation explored citric acid crosslinked hydroxyethyl tamarind gum hydrogel films as a potential biomaterial for drug delivery. Hydroxyethylation of tamarind gum aimed to improve its solubility, swelling, and crosslinking potential. The synthesized hydroxyethylated tamarind gum (HETG) was comprehensively characterized, revealing the presence of hydroxyethyl groups and increased viscosity in comparison to unmodified tamarind gum. The citric acid crosslinked HETG hydrogel films were developed by esterification-crosslinking mechanism. The films were characterized using instrumental techniques and evaluated for total carboxyl content, mechanical properties, swelling behavior, drug loading, drug release, antibacterial activity, hemocompatibility and in vitro wound healing activity. The presence of ester crosslinks and extent of crosslinking was confirmed through total carboxyl content and instrumental analysis. Varying HETG (2-2.5%w/v) and citric acid (1-1.4 %w/v) concentrations resulted in films with tunable mechanical strength, swelling, and drug loading. The films effectively controlled the release of a water-soluble drug (80.87-99.70 % in 24 h) through a non-Fickian diffusion mechanism. The optimized HETG hydrogel film showed antimicrobial activity, hemocompatibility, and support for cell growth, confirming its biocompatibility and potential for wound healing. Citric acid-crosslinked HETG films appear promising for drug delivery to wounds, meriting further in vivo study.
Formulation and evaluation of transdermal niosomal gel for antihyperlipidemic agent
Pravin Patil, Priyanka Bhagwat, Pournima Sankpal, Sachinkumar Patil, Shashikant Dhawale
Nanoscience and Nanotechnology Asia, 2024
Aims: The current study aims to create a formulation of Fluvastatin sodium (FVS) loaded niosome for the treatment of antihyperlipidemia using thin film hydration. The developed formulations were statistically optimized by two factors, three levels by 3-level factorial design and were evaluated for vesicle size, entrapment efficiency, zeta potential, transmission electron microscopy, and in-vitro drug release. Methods: The optimized FVS niosome being transformed to gel formulation was likewise analyzed for in-vitro skin permeability study, lipase action, and stability study. Results: The composition of an improved FVS niosome revealed vesicle size, entrapment effectiveness, zeta potential of 105.3 ± 12.4nm, 74.5 ± 0.86% and -36.2 ± 7mV, respectively, with spherical morphology. Conclusion: The FVS Niosomal gel demonstrated improved skin permeation compared to Orlistat. Furthermore, lipase activity showed better activity when compared with standard Orlistat drugs. Niosomal particles were discovered as a reliable nanovesicular carrier for the transdermal administration of FVS.
In-silico elucidation of phytoconstituents against 1LPB protein and anti-dyslipidaemic activity of Psoralea corylifolia Linn leaf extract
Pushpa A. Karale, Shashikant C. Dhawale, Mahesh A. Karale
Advances in Traditional Medicine, 2023
Synthesis and characterization of citric acid crosslinked carboxymethyl tamarind gum-polyvinyl alcohol hydrogel films
Kailas Krishnat Mali, Vishwajeet Sampatrao Ghorpade, Remeth Jacky Dias, Shashikant C. Dhawale
International Journal of Biological Macromolecules, 2023
SULFOXIDES AND SULFONES: REVIEW
Department of Pharmaceutical Chemistry, Womens College of Pharmacy, Peth-Vadgaon, Kolhapur – 416 112, Maharashtra, India, Satwashila S. Kadam, Niranjan S. Mahajan, Pankaj A. Jadhav, Shashikant C. Dhawale
Indian Drugs, 2023
It has been established that sulfoxide with sulfones have distinct pharmacological effects. Commodity compounds like sulfoxide and sulfones find widespread use in many chemical disciplines. This is why organic chemists find the synthesis of sulfoxide and sulfones so interesting. In the process of oxidation, sulphides can transform into sulfoxides or sulfones. Comprehensive oxidation to the sulfones is significantly simpler than mild oxidation to the sulfoxide, but both can be achieved by the use of highly selective technologies.
Concurrent oral delivery of non-oncology drugs through solid self-emulsifying system for repurposing in hepatocellular carcinoma
Rameshwar M. Ardad, Arehalli S. Manjappa, Shashikant C. Dhawale, Popat S. Kumbhar, Yogesh V. Pore
Drug Development and Industrial Pharmacy, 2023
Objective The present study aimed to identify a safe and effective non-oncology drug cocktail as an alternative to toxic chemotherapeutics for hepatocellular carcinoma (HCC) treatment. The assessment of cytotoxicity of cocktail (as co-adjuvant) in combination with chemotherapeutic docetaxel (DTX) is also aimed. Further, we aimed to develop an oral solid self-emulsifying drug delivery system (S-SEDDS) for the simultaneous delivery of identified drugs.Significance The identified non-oncology drug cocktail could overcome the shortage of anticancer therapeutics and help to reduce cancer-related mortality. Moreover, the developed S-SEDDS could be an ideal system for concurrent oral delivery of non-oncology drug combinations.Methods The non-oncology drugs (alone and in combinations) were screened in vitro for anticancer effect (against HepG2 cells) using (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide; MTT) dye assay, and cell cycle arresting and apoptotic behaviors using the fluorescence-activated cell sorting (FACS) technique. The S-SEDDS is composed of drugs such as ketoconazole (KCZ), disulfiram (DSR), tadalafil (TLF), and excipients like span-80, tween-80, soybean oil, Leciva S-95, Poloxamer F108 (PF-108), and Neusilin® US2 (adsorbent carrier), which was developed and characterized.Results The cocktail composed of KCZ, DSR, and TLF has showed substantial cytotoxicity (at the lowest concentration of 3.3 pmol), HepG2 cell arrest at G0/G1 and S phases, and substantial cell death via apoptosis. The DTX inclusion into this cocktail has further resulted in increased cytotoxicity, cell arrest at the G2/M phase, and cell necrosis. The optimized blank liquid SEDDS that remains transparent without phase separation for more than 6 months is used for the preparation of drug-loaded liquid SEDDS (DL-SEDDS). The optimized DL-SEDDS with low viscosity, good dispersibility, considerable drug retention upon dilution, and smaller particle size is further converted into drug-loaded solid SEDDS (DS-SEDDS). The final DS-SEDDS demonstrated acceptable flowability and compression characteristics, significant drug retention (more than 93%), particle size in nano range (less than 500 nm), and nearly spherical morphology following dilutions. The DS-SEDDS showed substantially increased cytotoxicity and Caco-2 cell permeability than plain drugs. Furthermore, DS-SEDDS containing only non-oncology drugs caused lower in vivo toxicity (only 6% body weight loss) than DS-SEDDS containing non-oncology drugs with DTX (about 10% weight loss).Conclusion The current study revealed a non-oncology drug combination effective against HCC. Further, it is concluded that the developed S-SEDDS containing non-oncology drug combination alone and in combination with DTX could be a promising alternative to toxic chemotherapeutics for the effective oral treatment of hepatic cancer.
LC-HRMS Analysis and Antihyperlipidemic Effect of Ethanolic Leaf Extract of Momordica charantia L.
Pushpa KARALE, Shashikant DHAWALE, Mahesh KARALE
Hacettepe University Journal of the Faculty of Pharmacy, 2022
Momordica charantia L. (Bitter melon) has been used to treat hyperglycemia and hyperlipidemia in many parts of the world. The present study revealed antihyperlipidemic activity of ethanolic leaf extract of M. charantia L. (named as EMC). The ethanolic extract of leaves were prepared and phytochemical constituents were identified using liquid chromatography linked with mass spectrophotometry. LCMS study indicates the presence of phenolic compound (m-hydroxy benzoic acid, octyl gallate, 3-hydroxycoumarin), triterpenoids (momordicin II, momordicoside E and momordicoside K), saponin E and fatty acids were the major constituents in EMC. Whereas, Triton X-100 induced hyperlipidemic rats model was used to evaluate antihyperlipidemic activity of EMC at a dose of 50, 100 and 200 mg/kg, b.w. or atorvastatin (10 mg/kg, b.w.). The plasma total cholesterol, triglycerides, HDL, LDL and VLDL level, hepatic cholesterol and triglyceride, fecal cholesterol and triglycerides level were checked. Triton X-100 significantly (P < 0.01) increased the serum total cholesterol, triglycerides and LDL with a concomitant reduction in HDL cholesterol. These alterations were ameliorated by EMC at dose dependant manner. EMC (200 mg/kg, b.w) showed significant (P < 0.01) reduction in lipid level among three doses of extracts in comparison with the standard drug atorvastatin. Overall results findings suggest that bitter melon may have potential to use as supplementary ingredient for the prevention of hyperlipidemia and related conditions.
Amelioration of diabetes and its complications by Manilkara zapota (L) P. Royen fruit peel extract and its fractions in alloxan and STZ-NA induced diabetes in Wistar rats
Pravin P. Karle, Shashikant C. Dhawale, Vijay V. Navghare
Journal of Diabetes and Metabolic Disorders, 2022
Quantitative Phytochemical Profile, Antioxidant and Lipase Inhibitory Potential of Leaves of Momordica charantia L. and Psoralea corylifolia L
Pushpa Karale, S. C. Dhawale, M. A. Karale
Indian Journal of Pharmaceutical Sciences, 2022
Antiarthritic potential of calotropis procera leaf fractions in fca-induced arthritic rats: Involvement of cellular inflammatory mediators and other biomarkers
Vandana S. Singh, Shashikant C. Dhawale, Faiyaz Shakeel, Md. Faiyazuddin, Sultan Alshehri
Agriculture Switzerland, 2021
Antiobesity potential and complex phytochemistry of momordica charantia linn. With promising molecular targets
Pushpa Karale, S. C. Dhawale, M. A. Karale
Indian Journal of Pharmaceutical Sciences, 2020
Extraction, characterization and functionalization of tamarind gum
Kailas K. Mali, Shashikant C. Dhawale, Remeth J. Dias
Research Journal of Pharmacy and Technology, 2019
Development of spray dried ritonavir microparticles for dissolution rate enhancement
Pravin S. Patil, Shashikant C. Dhawale
Research Journal of Pharmacy and Technology, 2018
Citric acid crosslinked carboxymethyl cellulose-based composite hydrogel films for drug delivery
K K Mali, S C Dhawale, R J Dias, N S Dhane, V S Ghorpade
Indian Journal of Pharmaceutical Sciences, 2018
Design, synthesis and biological screening of N-(substituted pyridine-2-yl)-N-(quinoline-2-yl) malonamide as novel anti-HIV-I agents
Indian Journal of Chemistry Section B Organic and Medicinal Chemistry, 2018
Development of vancomycin-loaded polysaccharide-based hydrogel wound dressings: In vitro and in Vivo evaluation
Asian Journal of Pharmaceutics, 2018
Development of ritonavir loaded nanoparticles: In vitro and in vivo characterization
Pravin S Patil, Shashikant C Dhawale
Asian Journal of Pharmaceutical and Clinical Research, 2018
Protective effects of ethanolic extract of Piper cubeba L. On D-galactose induced neuronal lipofuscinogenesis in albino rats
Muchandi, Ashok A., Shashikant C. Dhawale
Science Engineering and Health Studies, 2018
Synthesis and characterization of hydrogel films of carboxymethyl tamarind gum using citric acid
Kailas K. Mali, Shashikant C. Dhawale, Remeth J. Dias
International Journal of Biological Macromolecules, 2017
Interpenetrating networks of carboxymethyl tamarind gum and chitosan for sustained delivery of aceclofenac
Kailas Krishnat MALI, Shashikant C. DHAWALE, Remeth J. DIAS, Vijay D. HAVALDAR, Pankaj R. KAVITAKE
Marmara Pharmaceutical Journal, 2017
Suppression of Type-II Diabetes with Dyslipidemia and Nephropathy by Peels of Musa cavendish Fruit
Vijay Navghare, Shashikant Dhawale
Indian Journal of Clinical Biochemistry, 2016
3D QSAR and pharmacophore modeling studies on C-2 and C-4 substituted Quinazoline series as Anti-tubercular agents
Der Pharmacia Lettre, 2016
Design and optimization of modified tamarind gum-based floating-bioadhesive tablets of verapamil hydrochloride
Asian Journal of Pharmaceutics, 2016
Antifungal Activity of Helicteres isora Linn. Fruit Extracts Against Planktonic and Biofilm Growth of Candida albicans
Mahesh B. Manke, Jayant S. Raut, Shashikant C. Dhawale, S. Mohan Karuppayil
Journal of Biologically Active Products from Nature, 2015
Linked pyridinyl-thiadiazoles: Design and synthesis as potential candidate for treatment of XDR and MDR tuberculosis
Niranjan S. Mahajan, S.C. Dhawale
European Journal of Medicinal Chemistry, 2015
Alternatives to animal testing: A review
Sonali K. Doke, Shashikant C. Dhawale
Saudi Pharmaceutical Journal, 2015
Anthelmintic potential of Helicteres isora bark extract against Pheretima posthuma
Mahesh Bandappa Manke, Shashikant Chaburao Dhawale, Prasad Govindrao Jamkhande
Asian Pacific Journal of Tropical Disease, 2015
Development and validation of reversed phase HPLC method for the determination of vildagliptin using an experimental design
A. M. Kashid, D. A. Ghorpade, P. P. Toranmal, S. C. Dhawale
Journal of Analytical Chemistry, 2015
Helminthiasis and medicinal plants: A review
Mahesh Bandappa Manke, Shashikant Chaburao Dhawale, Prasad Govindrao Jamkhande
Asian Pacific Journal of Tropical Disease, 2015
In-vitro Anthelmintic and Antioxidant Activity of Helicteres isora Linn. Fruit Extracts
Mahesh B. Manke, Shashikant C. Dhawale, Dasrao A. Patil, Sanjay S. Pekamwar, Prasad G. Jamkhande
Journal of Biologically Active Products from Nature, 2015
Nasal mucoadhesive in-situ gel of granisetron hydrochloride using natural polymers
Kailas Mali, Shashikant Dhawale, Remeth Dias, Vijay Havaldar, Vishwajeet Ghorpade, et al.
Journal of Applied Pharmaceutical Science, 2015
Synthesis, Biological evaluation and docking study of 2-amino-4,6-diarylpyrimidines as novel non-nucleoside HIV-1reverse transcriptase inhibitors
Journal of Chemical and Pharmaceutical Research, 2014
Sensitization of candida albicans biofilms to fluconazole by terpenoids of plant origin
Sonali Kashinath Doke, Jayant Shankar Raut, Shashikant Dhawale, Sankunny Mohan Karuppayil
Journal of General and Applied Microbiology, 2014
Development and validation of a HPLC analytical assay method for dapoxetine tablets: A medicine for premature ejaculation
Journal of Chemical and Pharmaceutical Research, 2014
Therapeutic approaches to drug targets in atherosclerosis
Prasad G. Jamkhande, Prakash G. Chandak, Shashikant C. Dhawale, Sonal R. Barde, Priti S. Tidke, et al.
Saudi Pharmaceutical Journal, 2014
Synthesis, biological screening and ADME prediction of benzylindole derivatives as novel anti-HIV-1, anti-fungal and anti-bacterial agents
A. M. Kashid, P. N. Dube, P. G. Alkutkar, K. G. Bothara, S. N. Mokale, et al.
Medicinal Chemistry Research, 2013
Physicochemical characterization of spray dried ternary micro-complexes of cefuroxime axetil with hydroxypropyl-β-cyclodextrin
Manali Shah, Yogesh Pore, Shashikant Dhawale, Kishorkumar Burade, Bhanudas Kuchekar
Journal of Inclusion Phenomena and Macrocyclic Chemistry, 2013
A review: Biological importance of mercapto substituted 1,2,4-triazole derivatives
Research Journal of Pharmacy and Technology, 2012
Improvement in physicochemical properties of ezetimibe using a crystal engineering technique
Snehal P. Mulye, Samina A. Jamadar, Poonam S. Karekar, Yogesh V. Pore, Shashikant C. Dhawale
Powder Technology, 2012
Taste masking technologies of pharmaceuticals
Research Journal of Pharmacy and Technology, 2011
Polymer-drug conjugates: Recent achievements
Research Journal of Pharmaceutical Biological and Chemical Sciences, 2011
Formulation and evaluation porous microspheres of 5- fluorouracil for colon targeting
International Journal of Pharmtech Research, 2010
Floating drug delivery for controlled release of acyclovir
Indian Drugs, 2009
Behavior of suspending and wetting agents in aqueous environment
ShashikantC Dhawale, SudhirG Wadodkar, AvinashK Dorle
Asian Journal of Pharmaceutics, 2009

Shashikant C Dhawale

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Scopus Publications