Antioxidative enzymes as markers for the selection of advanced sweet potato breeding lines under in vitro osmotic stress conditions Ananya Mishra, Pradyumna Tripathy, Hanume Gowda Krishnappa, Madhumita Dasgupta, Sansuta Mohanty, Satyapriya Singh, Vijay Bahadur Singh Chauhan, Rameshkumar Arutselvan, Bibhuti Bhusan Sahoo, Manas Ranjan Sahoo Frontiers in Plant Science, 2026 Five advanced breeding lines of sweet potato were assessed for polyethylene glycol (PEG)-6000-mediated osmotic stress tolerance in vitro . Significant variation among the morphophysiological properties and antioxidative enzyme activities was observed under different levels of PEG (0, 0.1, and 0.2 MPa) incorporated in Murashige and Skoog (MS) medium. An induction of antioxidative enzymes—superoxide dismutase (SOD, Enzyme Commission [EC] 1.15.1.1), catalase (CAT, EC 1.11.1.6), ascorbate peroxidase (APX, EC 1.11.1.1), guaiacol peroxidase (GPX, EC 1.11.1.7), monodehydroascorbate reductase (MDHAR, EC 1.6.5.4), dehydroascorbate reductase (DHAR, EC 1.8.5.1), glutathione reductase (GR, EC 1.6.4.2), and polyphenol oxidase (PPO, EC 1.14.18.1)—was observed under stress compared to the control, and this induction was pronounced in the tolerant genotypes than in the susceptible ones. Among the antioxidant enzymes, CAT showed a strong positive correlation with GPX (Pearson’s correlation coefficient [ r ] = 0.73), whereas MDHAR was strongly and positively correlated with APX ( r = 0.73) and PPO ( r = 0.68). A significant increase in antioxidative enzyme activities was associated with lower growth retardation, as evident from the correlation study. Genotypes SP–30, followed by SP–18, possessed high principal component (PC1) scores and were rich in antioxidative enzymes, whereas genotypes SP–24, SP–26, and SP–28 exhibited lower enzyme activities and skewed morphological traits. The overall pattern of osmotic stress tolerance among the tested advanced sweet potato breeding lines followed the order: SP–30 > SP–18 > SP–26 > SP–24 > SP–28. The outcome of the study encourages the advancement of SP–30 for inclusion in future breeding strategies and/or its release following the official variety release procedures.
Harboring potential of enzymatic antioxidants in sweet potato [Ipomoea batatas (L.) Lam.] nodal cultures under in vitro NaCl-mediated salinity stress conditions Ananya Mishra, Madhumita Dasgupta, Sansuta Mohanty, Pradyumna Tripathy, Hanume Gowda Krishnappa, Satyapriya Singh, Vijay Bahadur Singh Chauhan, Rameshkumar Arutselvan, Bibhuti Bhusan Sahoo, Manas Ranjan Sahoo Frontiers in Plant Science, 2026 Salinity stress hinders the production and productivity of sweet potato worldwide. Stringent selection under in vitro salinity conditions would be a nebulous approach to developing stress-tolerant lines. Eight pre-breeding sweet potato genotypes, SP-12, SP-13, SP-23, SP-27, SP-33, SP-42, SP-44, and SP-45, selected from a broad genetic base of 380 germplasms, were evaluated under NaCl-mediated salinity stress conditions using nodal cultures in vitro . Sweet potato nodal cultures were raised in Murashige and Skoog (MS) medium with different levels of salinity (0, 50, and 100 mM). The morphological, physiological, and antioxidative enzyme activities under control conditions and salinity stress were assessed. Shoot and root organogenesis under the NaCl-induced MS medium (100 mM) were consistent in genotype SP-13. The antioxidative enzymes increased significantly [0.1-fold in catalase (CAT) to 2.7-fold in ascorbate peroxidase (APX) and guaiacol peroxidase (GPX)] with increasing salinity stress conditions compared to the control across the genotypes. Genotypes SP-13, SP-12, and SP-42 exhibited a higher stress tolerance index (STI) for antioxidative enzymes (AOEs). Pearson’s correlation coefficient (r) matrix revealed a strong integration among the growth parameters and AOEs. Among the antioxidative enzymes, APX (r = 0.74) and dehydroascorbate reductase (DHAR; r = 0.60) showed strong and positive correlations with glutathione reductase (GR). Polyphenol oxidase (PPO) exhibited a strong negative correlation with shoot parameters, including shoot length (r = −0.74) and shoot number (r = −0.71), indicating its association with shoot retardation. Principal component analysis (PCA) and hierarchical clustering indicated that genotype SP-13, followed by genotypes SP-12 and SP-42, is closely related to higher growth performances and better antioxidative enzyme mechanisms. Overall, SP-13, SP-12, and SP-42 performed well, maintaining plantlet growth and AOE properties. The results encourage the rapid screening of the more extensive pre-breeding populations to streamline breeding strategies and develop salinity-tolerant plants.
Sucrose supplementation influences gut microbial diversity and functional shifts in Apis cerana indica Deeptismita Nayak, Pravasini Behera, Satyapriya Singh, Partha Sarathi Tripathy, Soumya Shephalika Dash, Madhumita Dasgupta, Sansuta Mohanty, Manas Ranjan Sahoo, Chitta Ranjan Satapathy, Arundhati Sasmal, Subhasmita Sahu Frontiers in Microbiology, 2026 Honeybee colonies are increasingly threatened by nutritional scarcity and biotic stressors, underscoring the need to understand the role of gut microbiota in mitigating these challenges. This study examined the gut microbial composition of Apis cerana indica under two dietary regimes: sucrose–fed and sucrose–unfed, to assess how nutrition influences microbial diversity and metabolic potential following metagenomics. Metagenomic sequencing of gut samples revealed 147,146 contigs, with the longest and shortest contigs measuring 615,154 kb and 200 kb, respectively. Comparative analysis indicated a higher relative abundance of Bacillus spp. in sucrose–fed bees, whereas Enterococcus was more dominant in unfed populations. Sucrose feeding significantly enhanced gut microbial diversity (Shannon index: 2.59; Simpson’s index: 0.87) compared to unfed bees (Shannon: 1.91; Simpson: 0.68). Key genera, including Gilliamella , Bacillus , and Lactobacillus , were consistently present but showed varying relative abundances. Functional annotation via KEGG pathway analysis revealed elevated activity of glycolysis and the pentose phosphate pathway in sucrose-fed bees, with exclusive detection of key metabolic enzymes, hexokinase and enolase. Additionally, elevated sucrose metabolism and proteolytic enzyme activity were noted, reflecting enhanced metabolic versatility. Our findings highlight the importance of sucrose dietary supplementation in shaping gut microbial structure and function, their diversity, and metabolic capacity, suggesting its potential as a practical nutritional intervention to sustain honeybee health during a period of floral dearth. The outcome of the study encourages exploring the long–term ecological and physiological impacts of dietary strategies on colony resilience and productivity.
Influence of gamma irradiation on germination traits, growth and biochemical attributes of dragon fruit (Selenicereus monacanthus) Kundan Kishore, Kuldeep K. Shukla, Manas R. Sahoo, Kedareswar Pradhan, Rajkumari Bhol, Subash C. Swain Radiochimica Acta, 2024 The aim of this research is to assess the effectiveness of gamma irradiation in developing genetic variability in dragon fruit by influencing biochemical attributes. Seeds of dragon fruit were exposed to 100, 200, 300, 400 and 500 Gy γ-radiation using Co-60 source. Highest germinability, mean germination time, mean daily germination, vigour index and growth rate were observed in untreated seeds, however these traits were relatively less affected at lower irradiation level (up to 200 Gy). An irradiation level of ≥ 400 Gy severely affected germination traits and seedling growth and consequently 441 Gy irradiation level was considered as the lethal dose (LD50) for dragon fruit. Photosynthetic pigments, protein content and nutrient content were highest in untreated seedlings and moderately high up to 200 Gy. Findings demonstrated that the concentration of biochemical markers for abiotic stress tolerance viz. proline, phenol, flavonoid and antioxidant enzymes (superoxide dismutase, catalase and ascorbate peroxidase), were significantly high at 200 Gy irradiation level. Hence an irradiation dose of 200 Gy may be considered as an optimal for induced mutagenesis for abiotic stress tolerance in dragon fruit with the least possible unintended damage to seedling growth traits. The findings provide valuable insights into the efficient utilization of γ-irradiation in expediting the development of abiotic stress-tolerant mutant lines of dragon fruit.
Insect pest management strategies in organic vegetable cultivation Advances in Organic Farming Crop Production and Management, 2024
Nutrient, antioxidant and anti nutrient composition of value added products made with underutilized forest produce bay leaf (Cinnamomum tamala) Agricultural Engineering International Cigr Journal, 2020
Multivariate source apportionment and integrated indexical assessment of heavy metal contamination in northeast Indian wetland sediments and soils LD Sharma, I Bhupenchandra, EJ Singh, S Dutta, Y Pandey, ... Scientific Reports , 2026 2026
Harboring potential of enzymatic antioxidants in sweet potato [Ipomoea batatas (L.) Lam.] nodal cultures under in vitro NaCl-mediated salinity stress conditions A Mishra, M Dasgupta, S Mohanty, P Tripathy, HG Krishnappa, S Singh, ... Frontiers in Plant Science 17 (2026), https://doi.org/10.3389/fpls.2026.17409 , 2026 2026
Antioxidative enzymes as markers for the selection of advanced sweet potato breeding lines under in vitro osmotic stress conditions A Mishra, P Tripathy, HG Krishnappa, M Dasgupta, S Mohanty, S Singh, ... Frontiers in Plant Science 17 (1707715), doi.org/10.3389/fpls.2026.1707715 , 2026 2026
Sucrose supplementation influences gut microbial diversity and functional shifts in Apis cerana indica D Nayak, P Behera, S Singh, PS Tripathy, SS Dash, M Dasgupta, ... Frontiers in Microbiology 16 (1733283), doi.org/10.3389/fmicb.2025.1733283 , 2026 2026
Climate-resilient and biofortified tuber crop varieties V Chandra, K Pati, RA Shirly, MR Sahoo Indian Horticulture 70 (6), 11-16 , 2025 2025
Harnessing biodiversity for sustainable crop improvement in tropical tuber crops A Asha Devi, A KI, S KM, C Visalakshi, N Krishna Radhika, SN Rahana, ... Proceedings of ISTRTC, 63-75 , 2025 2025
Evaluation of gamma irradiation effect on morpho-physiological and biochemical traits of dragon fruit (Selenecereus monacanthus) under water stress environment KK Shukla, K Kishore, K Pradhan, SC Swain, MR Sahoo, R Bhol, S Samal, ... Plant Physiology Reports , 2025 2025 Citations: 3
The Unified Cycle from Stellar Nucleosynthesis to Food Crop Systems: Gratitude to Earth for Underground Tubers, Sweet Potato Leaves as Source of Energy-Nutrition A Mukherjee, P Bharadwaj, D Bharadwaj, MR Sahoo, AN Jyothi, ... Food and Nutrition Sciences 16 (8), 914-934 , 2025 2025
Phenotypic and genotypic variations among tomato (Solanum lycopersicum L.) cultivars within the ecologically sensitive Western undulating agroecosystem of India BB Sahoo, A Mohanty, BS Nayak, D Behera, M Dasgupta, MR Sahoo, ... Vegetos, Springer, 1-10 , 2025 2025
Harnessing genotype× environment interaction and advanced selection indices for nutritional enhancement in taro (Colocasia esculenta (L.) Schott) VBS Chauhan, HG Krishnappa, P Mohapatra, SN Mallick, ... Food Chemistry 473, 142938 , 2025 2025 Citations: 1
Genotype × Environment Interaction Analysis and Simultaneous Selection Using AMMI, BLUP, GGE Biplot and MTSI Under Drought Condition in Sweet Potato KH Gowda, VBS Chauhan, M Nedunchezhiyan, C Pradeepika, ... Journal of Soil Science and Plant Nutrition, https://doi.org/10.1007/s42729 … , 2025 2025 Citations: 4
Enhancing Adaptability, Nutritional Quality in Tropical Tuber Crops: Source for Adaptive Food and Nutrition A Mukherjee, J Sreekumar, MN Sheela, S Immanuel, MR Sahoo, V Hegde Food and Nutrition Sciences 15 (11), 1141-1169, DOI: 10.4236/fns.2024.1511074 , 2024 2024 Citations: 7
ENHANCING ADAPTABILITY AND WATER PRODUCTIVITY IN TROPICAL TUBER CROPS: FOOD SECURITY AND CLIMATE CHANGE PERSPECTIVES A Mukherjee, J Sreekumar, S Immanuel, MR Sahoo, M Nedunchezhiyan, ... J. Environ. & Sociobiol. 21 (1), 59-77 , 2024 2024
Breeding for Nutrient Enriched Coloured Flesh Sweet Potato (Ipomoea Batatas L.) with Endurance to Abiotic and Biotic Stresses--Revisiting the Past for Present and Emerging Future A Mukherjee, MR Sahoo, AN Jyothi, J Sreekumar, H Gowda, V Hegde, ... Advances in Agricultural Technology & Plant Sciences 7 (2), 1-14 , 2024 2024
Unlocking the Potential of Arbuscular Mycorrhizal Fungi: Exploring Role in Plant Growth Promotion, Nutrient Uptake Mechanisms, Biotic Stress Alleviation, and Sustaining … I Bhupenchandra, SK Chongtham, AG Devi, P Dutta, MR Sahoo, ... Journal of Plant Growth Regulation, doi.org/10.1007/s00344-024-11467-9 , 2024 2024 Citations: 100
Unveiling the Implications of Organic Nutrient Management Protocols on Soil Properties, Economic Sustainability, and Yield Optimization in Fenugreek Cultivation in Acidic Soils … C Tania, I Bhupenchandra, CP Devi, A Phonglosa, C Sonia, MN Singh, ... Sustainability 16 (7241), https://doi.org/ 10.3390/su16167241 , 2024 2024 Citations: 6
Influence of gamma irradiation on germination traits, growth and biochemical attributes of dragon fruit (Selenicereus monacanthus) K Kishore, KK Shukla, MR Sahoo, K Pradhan, R Bhol, SC Swain Radiochimica Acta, https://doi.org/10.1515/ract-2024-0294 , 2024 2024 Citations: 4
Exploring the morphological and biochemical characteristics of Kharif onion (Allium cepa L.): principal component and path coefficient analysis BB Sahoo, D Panda, A Panda, AJ Gupta, P Tripathy, N Senapati, ... Vegetos, 1-10 , 2024 2024 Citations: 2
Insect Pest Management Strategies in Organic Vegetable Cultivation S Singh, TK Bhoi, SK Sunani, S Majumder, P Srinivas, MR Sahoo, ... In book: Advances in Organic Farming: Crop Production and Management, 467 … , 2024 2024 Citations: 3
Harnessing weedy rice as functional food and source of novel traits for crop improvement I Bhupenchandra, SK Chongtham, A Gangarani, P Dutta, E Lamalakshmi, ... Plant Cell and Environment, https://doi.org/10.1111/pce.14868 , 2024 2024 Citations: 8
MOST CITED SCHOLAR PUBLICATIONS
Role of biostimulants in mitigating the effects of climate change on crop performance I Bhupenchandra, SK Chongtham, EL Devi, R R, AK Choudhary, ... Frontiers in plant science 13, 967665 , 2022 2022 Citations: 180
Unlocking the Potential of Arbuscular Mycorrhizal Fungi: Exploring Role in Plant Growth Promotion, Nutrient Uptake Mechanisms, Biotic Stress Alleviation, and Sustaining … I Bhupenchandra, SK Chongtham, AG Devi, P Dutta, MR Sahoo, ... Journal of Plant Growth Regulation, doi.org/10.1007/s00344-024-11467-9 , 2024 2024 Citations: 100
RAPD, ISSR, and SCoT markers based genetic stability assessment of micropropagated Dendrobium fimbriatum Lindl. var. oculatum Hk. f.-an important endangered orchid L Tikendra, AM Potshangbam, A Dey, TR Devi, MR Sahoo, P Nongdam Physiology and Molecular Biology of Plants, https://doi.org/10.1007/s12298 … , 2021 2021 Citations: 96
Evaluation of orange-fleshed sweet potato ( Ipomoea batatas L.) genotypes for salt tolerance through shoot apex culture under in vitro NaCl mediated salinity stress … M Dasgupta, MR Sahoo, PC Kole, A Mukherjee Plant Cell, Tissue and Organ Culture 94 (2), 161-170 , 2008 2008 Citations: 75
Antioxidative enzymes and isozymes analysis of taro genotypes and their implications in Phytophthora blight disease resistance MR Sahoo, M DasGupta, PC Kole, JS Bhat, A Mukherjee Mycopathologia 163 (4), 241-248 , 2007 2007 Citations: 57
Reactive oxygen species scavenging mechanisms associated with polyethylene glycol mediated osmotic stress tolerance in Chinese potato MR Sahoo, RD Tongbram, M Dasgupta, P Nongdam, N Prakash Scientific Reports 10 (5404), https://doi.org/10.1038/s41598-020-62317 , 2020 2020 Citations: 45
Understanding how silicon fertilization impacts chemical ecology and multitrophic interactions among plants, insects and beneficial arthropods TK Bhoi, I Samal, DK Mahanta, J Komal, D Jinger, MR Sahoo, GC Achary, ... Silicon 15 (6), 2529-2549 , 2023 2023 Citations: 34
Molecular Phylogeny, DNA Barcoding, and ITS2 Secondary Structure Predictions in the Medicinally Important Eryngium Genotypes of East Coast Region of India GC Acharya, S Mohanty, M Dasgupta, S Sahu, S Singh, AVV Koundinya, ... Genes 13 (9), 1678 , 2022 2022 Citations: 30
Changes in Phenolics, Polyphenol Oxidase and its Isoenzyme Patterns in Relation to Resistance in Taro against Phytophthora colocasiae MR Sahoo, PC Kole, M Dasgupta, A Mukherjee Journal of Phytopathology 157 (3), 145-153 , 2009 2009 Citations: 24
Indigenous plant protection practices of Tripura, India S Singh, B Das, A Das, S Majumder, HL Devi, RS Godara, AK Sahoo, ... Journal of Ethnobiology Ethnomedicine 17 (50), https://doi.org/10.1186 … , 2021 2021 Citations: 23
Effect of in vitro and in vivo induction of polyethelene glycol-mediated osmotic stress on hybrid taro (Colocasia esculenta (L.) Schott) MR Sahoo, M DasGupta, A Mukherjee Annals of tropical research 28 (2), 1-11 , 2006 2006 Citations: 22
An efficient protocol for in vitro regeneration and conservation of Shirui lily (Lilium mackliniae Sealy): a lab-to-land approach to save the rare endangered Asiatic lily species. MR Sahoo, MP Devi, M Dasgupta, N Prakash, SV Ngachan In Vitro Cellular & Developmental Biology Plant 54 (6) , 2018 2018 Citations: 21
Repercussions of fertilization with boron and enriched organic manure on soil chemical characteristics, boron and phosphorus fractions, and French bean productivity in an … I Bhupenchandra, A Basumatary, S Dutta, A Das, AK Choudhary, R Lal, ... Scientia Horticulturae 324 (2024), https://doi.org/10.1016/j.scienta.2023.1 , 2023 2023 Citations: 20
Phytochemical profiling of spiny coriander ( Eryngium foetidum L.) – A potential perennial spicing-culinary herb of eastern India GC Acharya, N Ponnam, M Kumari, TK Roy, KS Shivashankara, ... Acta Chromatographica 34 (2), 197-202 , 2021 2021 Citations: 19
Fortification of ready–to–eat extruded snacks with tree bean powder: nutritional, antioxidant, essential amino acids, and sensory properties MR Sahoo, A Kuna, MP Devi, M Sowmya, M Dasgupta Journal of Food Science and Technology 59 (6), 2351-2360 , 2022 2022 Citations: 18
Photosynthetic, physiological and biochemical events associated with polyethylene glycol-mediated osmotic stress tolerance in taro (Colocasia esculenta L. Schott) MR SAHOO, M DASGUPTA, PC KOLE, A MUKHERJEE Photosynthetica 56 , 2018 2018 Citations: 18
Reactive oxygen species turnover, phenolics metabolism, and some key gene expressions modulate postharvest physiological deterioration in cassava tubers ED Wahengbam, CP Devi, SK Sharma, SS Roy, A Maibam, M Dasgupta, ... Frontiers in Microbiology 14, 1148464 , 2023 2023 Citations: 17
Biochemical Changes in Leaf Tissues of Taro [ Colocasia esculenta L. (Schott)] Infected with Phytophthora colocasiae MR Sahoo, M Dasgupta, PC Kole, A Mukherjee Journal of Phytopathology 158 (3), 154-159 , 2010 2010 Citations: 17
Elucidating the impact of boron fertilization on soil physico-chemical and biological entities under cauliflower-cowpea-okra cropping system in an eastern Himalayan acidic … I Bhupenchandra, A Basumatary, AK Choudhary, A Kumar, D Sarkar, ... Frontiers in Microbiology 13, 996220 , 2022 2022 Citations: 15
Silicon: a potent nutrient in plant defense mechanisms against arthropods S Singh, MR Sahoo, GC Acharya, D Jinger, P Nayak Silicon 14 (12), 6493-6505 , 2022 2022 Citations: 15