@uho.ac.id
Food Science and Technology
Universitas Halu Oleo
PhD Student
Food Science, Biochemistry
Scopus Publications
Scholar Citations
Scholar h-index
Scholar i10-index
Holilah Holilah, Lisman Suryanegara, Hasliza Bahruji, Nanang Masruchin, Suprapto Suprapto, Ratna Ediati, Asranudin Asranudin, Aishah A. Jalil, Dini Viandi Ramadhani, Zuratul Ain Abdul Hamid,et al.
Elsevier BV
Agus Wedi Pratama, Tri Mulyono, Bambang Piluharto, Nurul Widiastuti, Melbi Mahardika, Badrut Tamam Ibnu Ali, Asranudin, Dalia Allouss, and Ilias El Alaoui-Elbalrhiti
Elsevier BV
Adi Setyo Purnomo, Surya Rosa Putra, Herdayanto Sulistyo Putro, Afan Hamzah, Nova Ainur Rohma, Alya Awinatul Rohmah, Hamdan Dwi Rizqi, Asranudin, Bieby Voijant Tangahu, I. D. A. A. Warmadewanthi,et al.
Royal Society of Chemistry (RSC)
Oil spills that contaminate the environment can harm the surrounding ecosystem.
Asranudin, Holilah, Adi Setyo Purnomo, Hasliza Bahruji, Dalia Allouss, Ilias El Alaoui-Elbalrhiti, Riki Subagyo, Alya Awinatul Rohmah, and Didik Prasetyoko
Royal Society of Chemistry (RSC)
Encapsulation of hectorite-modified CTAB with Ca-alginate formed reusable adsorbent beads for wastewater treatment.
Adi Setyo Purnomo, Alya Awinatul Rohmah, Weni Sri Ekowati, Hamdan Dwi Rizqi, and Asranudin Asranudin
Universitas Gadjah Mada
The disposal of dye wastewater has become a major global concern. Meanwhile, microorganisms have shown high potential in the treatment of wastewater pollutants. In this study, the involvement of the Fenton reaction in the biodecolorization and biodegradation of methylene blue (MB) by the brown rot fungus Daedalea dickinsii was investigated. Subsequently, D. dickinsii is a fungus capable of producing hydroxyl radicals (•OH). This experiment was conducted with an initial MB concentration of 75 mg/L, and different incubation times of 0, 7, 14, 21, and 28 d respectively. The result showed that the Fenton reaction played an important role, and this was demonstrated by the addition of FeSO4 as a Fe2+ source. The removal of MB by D. dickinsii with the addition of Fe2+ reached 91.454% at 28 d in a mineral salt medium. It was higher compared to D. dickinsii culture treatment without Fe2+ addition, 86.427%. Furthermore, the metabolic degradation product was analyzed using LC-TOF/MS and identified as 2-amino-3-hydroxy-5-(methylamino) benzenesulfonic acid and N-(3,4-dihydroxy phenyl)-N-methyl formamide.
Asranudin, Adi Setyo Purnomo, Didik Prasetyoko, Hasliza Bahruji, and Holilah
Elsevier BV
Dini Viandi Ramadhani, Holilah Holilah, Hasliza Bahruji, Nurul Jadid, Titie Prapti Oetami, Aishah Abdul Jalil, Asranudin Asranudin, Ratna Ediati, Nanang Masruchin, Lisman Suryanegara,et al.
Elsevier BV
Alvin Romadhoni Putra Hidayat, Alvin Rahmad Widyanto, Liyana Labiba Zulfa, Asranudin Asranudin, Djarot Sugiarso, Herdayanto Sulistyo Putro, Adi Setyo Purnomo, Didik Prasetyoko, Arif Priyangga, Lukman Atmaja,et al.
Elsevier BV
Asranudin, Sri Fatmawati, Adi Setyo Purnomo, Holilah, and Alya Awinatul Rohmah
AIP Publishing
Alvin Romadhoni Putra Hidayat, Alvin Rahmad Widyanto, Asranudin Asranudin, Ratna Ediati, Dety Oktavia Sulistiono, Herdayanto Sulistyo Putro, Djarot Sugiarso, Didik Prasetyoko, Adi Setyo Purnomo, Hasliza Bahruji,et al.
Journal of Environmental Chemical Engineering Elsevier BV
A. A. Rohmah, A.S. Purnomo, and Asranudin
IOP Publishing
Abstract Wastewater which is not handled by a proper treatment before released in the water body, can cause damage for aquatic biota and its environment. MB is a synthetic textile dye which is toxic and difficult to degrade. Bioremediation by decolorization using microbes such as bacteria is one of the best methods for dye removal. By immobilizing Bacillus subtilis bacterium in an appropriate matrix, it could have more advantage on dye decolorization than by free cell only. The sodium alginate (SA) and polyvinyl alcohol (PVA) are well known as good matrix for decolorization. Besides the addition of B. subtilis in the SA-PVA matrix, the addition of adsorbents could enhance the decolorization. In this study, different adsorbents: bentonite, activated charcoal, kaolin, and corncob powder were investigated for influencing MB decolorization by immobilized B. subtilis in SA-PVA matrix. The result showed that the highest MB decolorization was reached by SA-PVA-B. subtilis with the presence of activated charcoal by approximately 88% of 100 mg/L, followed by the addition of bentonite (82%), kaolin (80%), and corncob powder (79%). The MB decolorization by SA-PVA-Activated charcoal-B. subtilis reached optimum condition at temperature 35 °C, 24 h incubation time in static condition.
Holilah Holilah, Hasliza Bahruji, Ratna Ediati, Asranudin Asranudin, Aishah Abdul Jalil, Bambang Piluharto, Reva Edra Nugraha, and Didik Prasetyoko
International Journal of Biological Macromolecules Elsevier BV
Adi Setyo Purnomo, Asranudin, Nela Rachmawati, Hamdan Dwi Rizqi, Refdinal Nawfa, and Surya Rosa Putra
Bogor Agricultural University
The involvement of Fenton reaction on biodegradation of methyl orange (MO) by brown-rot fungus Fomitopsis pinicola was investigated based on Fe2+-dependent reaction. The degradation of MO (final concentration 75 mg/L) was performed in mineral salt media with and without Fe2+ with incubation period at 0, 7, 14, 21, and 28 days. Degradation analysis was performed using UV-Vis Spectrophotometer and LC-TOF/MS. F. pinicola decolorized MO in a medium containing Fe2+ and a medium that lacked the mineral, at percentages of 89.47% and 80.08%, respectively. The optimum decolorization occurred after 28 days of incubation with the fungus on the presence of Fe2+, indicated that the presence of Fe2+ enhanced MO degradation with assumed to correlate with Fenton reaction. Two metabolites were detected through the LC-TOF/MS analysis, namely 4-(2-(4-(dimethyliminio)-2-hydroxycyclohexa-2,5-dien-1-ylidene) hydrazinyl) phenolate (m/z 258, RT: 1.28 min, compound 1) and 4-(2-(4-(dimethyliminio) cyclohexa-2,5-dien-1-ylidene) hydrazinyl) benzenesulfonate (m/z 391, RT: 2.70 min, compound 2). Compound 1 was a transformation product of hydroxylation and methylation, compound 2 was a product of dehydroxylation and desulfonation. This study indicated that the transformation of the metabolite structures was involved hydroxyl radical (OH.) and enzymatic mechanisms, which involved Fe2+-dependent reaction.
Asranudin, Holilah, Andi Noor Kholidha Syarifin, Adi Setyo Purnomo, Ansharullah, and Ahmad Fudholi
Elsevier BV
Holilah Holilah, Didik Prasetyoko, Ratna Ediati, Hasliza Bahruji, Aishah Abdul Jalil, Asranudin Asranudin, and Susanti Dhini Anggraini
Journal of Cleaner Production Elsevier BV
Abstract Conversion of industrial pepper bio-waste (PW) to microcrystalline cellulose (MCC) was investigated as reinforcement agent of natural polymer bio-composites. The investigation highlighted the importance of hydrothermal assisted alkalization step for efficient separation of cellulose from lignin in PW in order to enhance the yield and the crystallinity of MCC. Cellulose was isolated from industrial pepper bio-waste via three steps i.e. alkalization, bleaching and hydrothermal assisted re-alkalization. Hydrothermal treatment during re-alkalization step increased the release of lignin that was bound within the cellulose domain via dissociation of the multiple bonds in the three-dimensional polymer and significantly reduced the lignin concentration. Optimization of hydrolysis parameters i.e. the concentration of HCl, the hydrolysis temperature and time, and also the solid/liquid ratios produced 86% of MCC with high crystallinity index ∼ 78.9%. The physicochemical properties of MCC isolated from PW exhibited a highly crystalline microcellulose structure with high thermal stability that were comparable to the physicochemical properties of the commercial MCC. Incorporation of MCC with seaweed and chitosan natural polymer showed the enhancement of the tensile strength and the elongation of the bio-composites, which exhibited the potential use of MCC as reinforcement agent in bio-composites to replace traditional plastic.
Adi Setyo Purnomo, Asranudin Asranudin, Didik Prasetyoko, and Yulinar Dwi Nur Azizah
Universitas Gadjah Mada
The biotransformation and biodecolorization of methylene blue (MB) dye using the bacterium Ralstonia pickettii was investigated. This experiment was conducted in a nutrient broth (NB) medium after adding MB at 100 mg L–1 concentration. Approximately 98.11% of MB was decolorized after 18 h of incubation. In addition, the metabolic products detected by LC-TOF/MS were Azure A (AA), thionine, leuco-MB, and glucose-MB, which indicated the MB degradation through a reductase that attacked the heterocyclic central chromophore group present in the structure. Moreover, azure A and thionine fragments resulted from the N-demethylase enzyme that attacked the auxochrome group. Thus, this research was assumed to be the first scientific report suggesting the potential to use R. pickettii in the biodecolorization and biotransformation of dye waste, particularly MB.
S Wahyuni, Holilah, Asranudin, M I K Rianse, and M S Sadimantara
IOP Publishing
S Wahyuni, Holilah, Asranudin, and Noviyanti
IOP Publishing
The shelf life of brownies cake made from wikau maombo flour was predicted by ASLT method through the Arrhenius model. The aim of this study was to estimate the shelf life of brownies cake made from wikau maombo flour. The storage temperature of brownies cake was carried out at 20°C, 30°C and 45°C. The results showed that TBA (Thio Barbaturic Acid) number of brownies cake decreased as the storage temperature increase. Brownies stored at 20°C and 30°C were overgrown with mold on the storage time of six days. Brownies product (WT0 and WT1) had shelf life at 40°C approximately six and fourteen days, respectively. Brownies made from wikau maombo and wheat flour (WT1) was the best product with had the longest of shelf life about fourteen days.
Sri Wahyuni, Ansharullah, Saefuddin, Holilah, and Asranudin
Springer Science and Business Media LLC
Wikau maombo flour has a great potential as an economical food products in Southeast Sulawesi. The aims of this study were to apply the traditional methods of cassava flour manufacturing process roomates including peeling, washing, the immersion of sea water, fermentation, drying, milling and analyze the characteristic of wikau Matombo flour. The cassava was immersed for 24 h in sea water prior to fermentation in 1–4 days. Fermented flour in 3 days is the choosen product due to its good organoleptic and mass-produced analysis. The cyanide content of the flour was 2.7 mg/kg, whereas the content of carbohydrate, protein, fat, ash, moisture and crude fiber were 87.69 ± 0.1, 2.4 ± 0.1, 0.34 ± 0.02, 1.04 ± 0.59, 6.23 ± 0.48 and 2.30 ± 0.72 %, respectively. FTIR analysis showed that the intensity of peaks in 1016, 1150, 1650, 2990 and 3390 cm−1 were found to decrease with a longer fermentation. Based on the XRD analysis, the crystallinity of Wikau maombo flour decreased as the fermentation time increase. With a long fermentation, the morphology of the flour was observed as irregular granules with starch granule size. The physicochemical properties of wikau Matombo flour including swelling power, crystallinity, solubility, cyanide content, viscosity, pasting temperature, pH and granule size were 8.39 ± 0.06 g/g, 24.41 %, 66 ± 0.27 %, 2.7 ± 0.03 mg/kg, 4061cP, 74.1 °C, 6.5 ± 0.05 and 5–15 μm, respectively.
S. Wahyuni, A. Khaeruni, A.S. Purnomo, Asranudin, Holilah, and Fatahu
Asian Journal of Chemistry
S. Wahyuni, M.T. Suhartono, A. Khaeruni, A.S. Purnomo, Asranudin, Holilah, and P.A. Riupassa
Asian Journal of Chemistry
S. Wahyuni, P.E. Susilowati, Tamrin, Holilah, Asranudin, W.P. Utomo, Y. Dueris, and L. Samarindu
Asian Journal of Chemistry
Green Pepper, Red Pepper
Pepper modification