Microbiology, Infectious Diseases, Immunology and Microbiology, Molecular Biology
4
Scopus Publications
Scopus Publications
Essential oil composition, in vivo antioxidant, and antimicrobial activities of pimpinella pruatjan from west Java, Indonesia Agustina D.R. Nurcahyanti, Issam J. Nasser, Frank Sporer, Bernhard Wetterauer, Ireng D. Kadarso, Jurgen Reichling, Michael Wink Natural Products Journal, 2018 Background: The root of the Indonesian Pimpinella pruatjan Molk. has been mainly used as an aphrodisiac and for the treatment of Erectile Dysfunction (ED). Since oxidative stress favors ED, we examined the antioxidant activities of the essential oil from the root of P. pruatjan. Methods: The chemical composition of the essential oil was analyzed by Gas Liquid Chromatography- Mass Spectrometry (GLC-MS). In vivo antioxidant activity was determined using an animal model, the nematode Caenorhabditis elegans, whereas the antimicrobial activity of the essential oil was investigated using standard micro-dilution methods. Results: The main components of essential oil were thymol methyl ether and 2,5-dimethoxy-pcymene; another major compound was tentatively identified as 2-isopropenyl-5-methyl-benzene-1,4- diol. Root essential oil showed substantial in vivo antioxidant activities, demonstrated by reduced hsp-16.2p::GFP expression in the pharynx of C. elegans and increased survival of worms under acute stress conditions. Antimicrobial activity showed moderate activity against bacteria and Candida yeasts. Conclusion: The antioxidant property shown in these findings needs further investigation, especially with the major active compounds. Keywords: Pimpinella pruatjan Molk., Apiaceae, essential oil, antioxidant, Caenorhabditis elegans, antimicrobial.
Mycobacterium tuberculosis lineage 4 comprises globally distributed and geographically restricted sublineages David Stucki, Daniela Brites, Leïla Jeljeli, Mireia Coscolla, Qingyun Liu, Andrej Trauner, Lukas Fenner, Liliana Rutaihwa, Sonia Borrell, Tao Luo, Qian Gao, Midori Kato-Maeda, Marie Ballif, Matthias Egger, Rita Macedo, Helmi Mardassi, Milagros Moreno, Griselda Tudo Vilanova, Janet Fyfe, Maria Globan, Jackson Thomas, Frances Jamieson, Jennifer L Guthrie, Adwoa Asante-Poku, Dorothy Yeboah-Manu, Eddie Wampande, Willy Ssengooba, Moses Joloba, W Henry Boom, Indira Basu, James Bower, Margarida Saraiva, Sidra E G Vasconcellos, Philip Suffys, Anastasia Koch, Robert Wilkinson, Linda Gail-Bekker, Bijaya Malla, Serej D Ley, Hans-Peter Beck, Bouke C de Jong, Kadri Toit, Elisabeth Sanchez-Padilla, Maryline Bonnet, Ana Gil-Brusola, Matthias Frank, Veronique N Penlap Beng, Kathleen Eisenach, Issam Alani, Perpetual Wangui Ndung'u, Gunturu Revathi, Florian Gehre, Suriya Akter, Francine Ntoumi, Lynsey Stewart-Isherwood, Nyanda E Ntinginya, Andrea Rachow, Michael Hoelscher, Daniela Maria Cirillo, Girts Skenders, Sven Hoffner, Daiva Bakonyte, Petras Stakenas, Roland Diel, Valeriu Crudu, Olga Moldovan, Sahal Al-Hajoj, Larissa Otero, Francesca Barletta, E Jane Carter, Lameck Diero, Philip Supply, Iñaki Comas, Stefan Niemann, Sebastien Gagneux Nature Genetics, 2016 Sebastien Gagneux and colleagues analyze a global collection of Mycobacterium tuberculosis clinical isolates to classify sublineages by phylogeography. They find globally distributed ‘generalist’ and geographically restricted ‘specialist’ sublineages of lineage 4, indicating that different evolutionary strategies were adopted to succeed in various ecological niches. Generalist and specialist species differ in the breadth of their ecological niches. Little is known about the niche width of obligate human pathogens. Here we analyzed a global collection of Mycobacterium tuberculosis lineage 4 clinical isolates, the most geographically widespread cause of human tuberculosis. We show that lineage 4 comprises globally distributed and geographically restricted sublineages, suggesting a distinction between generalists and specialists. Population genomic analyses showed that, whereas the majority of human T cell epitopes were conserved in all sublineages, the proportion of variable epitopes was higher in generalists. Our data further support a European origin for the most common generalist sublineage. Hence, the global success of lineage 4 reflects distinct strategies adopted by different sublineages and the influence of human migration.
Chemical composition of the essential oil from aerial parts of Javanian Pimpinella Pruatjan molk. And Its Molecular Phylogeny Agustina Nurcahyanti, Issam Nasser, Frank Sporer, Jürgen Graf, Nurliani Bermawie, Jürgen Reichling, Michael Wink Diversity, 2016 The species-rich and diverse genus Pimpinella is mainly distributed in Europe and Asia; a few species occur in Africa. Yet, the Javanian Pimpinella, P. pruatjan, which has been used as an aphrodisiac in Indonesian traditional medicine, was studied for the first time in the context of chemical composition, as well as phylogeny analysis and antimicrobial activity. We examined the chemical composition of the essential oil (EO) from aerial parts of P. pruatjan by gas liquid chromatography-mass spectrometry (GLC-MS). The main component of EO was (Z)-γ-bisabolene. Several oxygenated monoterpenes, oxygenated sesquiterpenes, and sesquiterpenes were also detected. The genetic relationship of Pimpinella pruatjan Molk. to other Pimpinella species was reconstructed using nucleotide sequences of the nuclear DNA marker ITS (Internal Transcribed Spacer). P. pruatjan clusters as a sister group to the African Pimpinella species. The EO did not exhibit an apparent antimicrobial activity.
