ORBELIN SOBERANIS RAMOS

@unam.mx

Department of Preventive Medicine and Public Health,
National Autonomous University of Mexico

ORBELIN SOBERANIS RAMOS


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https://researchid.co/osoberanis

RESEARCH, TEACHING, or OTHER INTERESTS

Veterinary, Veterinary, Veterinary, Veterinary
14

Scopus Publications

Scopus Publications

  • Long-term genomic surveillance reveals the circulation of clinically significant Salmonella in lymph nodes and beef trimmings from slaughter cattle from a Mexican feedlot
    Enrique Jesús Delgado-Suárez, Abril Viridiana García-Meneses, Elfrego Adrián Ponce-Hernández, Francisco Alejandro Ruíz-López, Cindy Fabiola Hernández-Pérez, Nayarit Emérita Ballesteros-Nova, Orbelín Soberanis-Ramos, María Salud Rubio-Lozano
    Plos One, 2024
    This longitudinal study characterized Salmonella circulating in lymph nodes (LN, n = 800) and beef trimmings (n = 745) from slaughter cattle from a Mexican feedlot. During two years, LN and beef trimming samples were collected 72–96 h post-slaughter, and we obtained 77 isolates of the serovars Anatum (n = 23), Reading (n = 22), Typhimurium (n = 10), London (n = 9), Kentucky (n = 6), Fresno (n = 4), Give, Muenster, and monophasic 1,4,[5],12:i- (n = 1 each). These isolates were subjected to whole genome sequencing, single-nucleotide polymorphism (SNP)-based phylogenetic analysis, reconstruction of their ancestral isolation sources through evolutionary analysis, and virulence profiling. Although LN and beef trimmings were not mixed, evolutionary analysis estimated that the common ancestor of all study isolates was likely of LN origin. Moreover, isolates from both sources were highly clonal (0–21 SNP distance), highlighting the complexity of Salmonella transmission dynamics. The pathogen persisted across cattle cohorts, as shown by clonality between isolates collected in different years (1–20 SNP distance). Major virulence genes were highly conserved (97–100% identity to the reference sequences) and most isolates carried a conserved version of pathogenicity islands 1–5, 9, 11, and 12. Typhimurium strains carried the Salmonella plasmid virulence operon (spvRABCD), and a Muenster isolate carried the st313td gene, both of which are associated with invasive phenotypes. Most isolates (49/77) were genetically similar (1–43 SNPs) to strains involved in human salmonellosis, highlighting their public health significance. Further research is needed on Salmonella transmission dynamics in cattle and the mechanisms determining subclinical infection and persistence in farm environments.
  • Seroprevalence of leptospirosis in a Mexican military population working with animals
    Juan Ramon Ayala‐Torres, María Fernanda Hernández‐Morales, Valeria María Alanis‐Gallardo, Laura Olivia Arvizu‐Tovar, Orbelin Soberanis‐Ramos
    Public Health Challenges, 2024
    BackgroundLeptospirosis is a zoonotic disease and a challenge to global public health. There is an occupational risk, particularly in populations with direct contact with animals and in high‐humidity environments, which favors the survival of leptospires. This study aimed to determine the seroprevalence of leptospirosis in military personnel working in close contact with animals in México and to describe the available preventive measures and protection levels.MethodsA cross‐sectional study was conducted from March to October 2015. Information regarding protective factors in daily activities was gathered through a self‐evaluation questionnaire. The serum samples of participants were analyzed through enzyme‐linked immunoassay (ELISA) and microscopic agglutination test.ResultsSerums were obtained from 65 active military personnel, 56 males (86.2%) and 9 females (13.8%). Out of the total, 54 (83.1%) tested positive for infection by leptospirosis, 49 were males (87.5%) and 5 were females (55.6%). The highest seroprevalence age group was in the ≥45 years group (15, 23.07%), where all tested positive. Regarding military ranks, 100% of the highest hierarchy turned out positive: Officers (4 out of 4) and Chiefs (14 out of 14); and troops resulted in a seroprevalence of 76.5%. Protection equipment available during daily chores included: Overall, 64.6% had gloves and 53.8% had boots; the reported frequency for the use of gloves was 35.3% (46/65) if worn during more than half of the workday, yet 29.2% (19/65) reported never wearing them.ConclusionsThis study makes the petition to implement protocols of continuous training regarding labor risks and having an epidemiologic surveillance program for exposed personnel indispensable to improve the health and sanitary conditions of military personnel who work in direct contact with animals.
  • Spatial patterns and risk of aggression of dogs and cats in a central township of Mexico City
    José Luis Quezada-Nolasco, Miguel Galarde-López, Luisa María Sánchez-Zamorano, Juan Manuel Serrano-Márquez, Laura Olivia Arvizu-Tovar, Antonio Reyna-Sevilla, Valeria María Alanis-Gallardo, Orbelín Soberanis-Ramos
    Acta Tropica, 2023
  • Quality evaluation of non-medicated commercial shampoos for dogs in Mexico City
    Sheila Irais Peña-Corona, Ivan Juárez-Rodríguez, Dinorah Vargas-Estrada, Laura Olivia Arvizu Tovar, Enrique Amador-González, Orbelín Soberanis Ramos
    Veterinaria Mexico Oa, 2022
    This work performed a descriptive analysis of the quality of shampoos for use in dogs that are marketed in Mexico City. Up to now, there is a lack of information in this regard, which is important to facilitate the selection and recommendation of these products by veterinary professionals. Products of 20 commercial brands were studied, to which physicochemical, sensory and performance tests were carried out, in addition to evaluating the content of their labels. The pH of the shampoos varied between 5.6 and 8.4; the viscosity, between 1 131 and 3 102 m Pa-s; the relative density, between 1.002 and 1.099 g/mL and the foam rate between 2.9 and 4.0 min. 95 % of the shampoos formed a closed foam, between 10 and 35 % of the brands presented deficiencies in the labeling. Generally, the physical-chemical and sensory properties of the shampoos brands under study are within the acceptable limits for the line of use in humans. However, the lack of consistency in labeling suggests that it is necessary to improve the supervision of applicable regulations to protect animals and public health.
  • Use of an extrapolation method to estimate the population of cats and dogs living at homes in Mexico in 2022
    Sheila Irais Peña-Corona, Jose Pablo Gomez-Vazquez, Evelyn Aditi López-Flores, Dinorah Vargas-Estrada, Laura Olivia Arvizu-Tovar, Juan José Pérez-Rivero, Iván Jua´rez-Rodríguez, Alonso Sierra Resendiz, Orbelín Soberanis Ramos
    Veterinaria Mexico Oa, 2022
    Cats and dogs have a very close relationship with humans. Currently, the overpopulation of these species in various countries worldwide has been identified as a severe public health problem. To establish effective programs for population control, it is necessary to estimate the number of cats and dogs. To our knowledge, there are no studies that assess the number of canine and feline population by state in Mexico. Therefore, this study aimed to estimate the canine and feline population living at homes using official information reported by the National Institute of Statistics, Geography and Informatics [Instituto Nacional de Estadística Geografía e Informática] through a constant-share model, a ratio extrapolation method. The estimated dog population living at homes was 42 625 010 dogs and 7 346 925 cats (at least 5.8 times less than the number of dogs). By 2022, the Mexico's estimated human:dog ratio was 2.4:1, and the human:cat ratio 17.6:1. The Estado de México had more than six million dogs and 962 177 cats. Colima had the smallest estimated dog population (245 489), and Baja California Sur the smallest estimated cat population (slightly over 30 000). Health authorities and veterinary practitioners should promote responsible ownership to increase veterinary care and control the birth of dogs and cats.
  • Genetic diversity of Mycobacterium bovis evaluated by spoligotyping and MIRU-VNTR in an intensive dairy cattle breeding area in Mexico
    Anabel Ordaz‐Vázquez, Orbelin Soberanis‐Ramos, Edith Cruz‐Huerta, Sandra Retis‐Sanchez‐de‐la‐Barquera, Bárbara Chávez‐Mazari, Tomasa Gudiño‐Enriquez, Mario Santacruz‐Aguilar, Alfredo Ponce‐De‐León‐Garduño, José Sifuentes‐Osornio, Miriam Bobadilla‐Del‐Valle
    Transboundary and Emerging Diseases, 2022
    Bovine tuberculosis (bTB) is mainly caused by Mycobacterium bovis. In Mexico, dairy cattle play an important role in the persistence and spread of the bacillus. In order to describe M. bovis genetic diversity, we genotyped a total of 132 strains isolated from slaughtered cattle with bTB suggestive lesions between 2009 and 2010 in Hidalgo, Mexico, using a panel of 9-loci mycobacterial interspersed repetitive unit-variable number of tandem repeats (MIRU-VNTR) and spoligotyping. We found 21 spoligotypes, and 124 isolates were grouped in 13 clusters. The most frequent spoligotypes were SB0121 (49, 37.1%) and SB0673 (27, 20.5%); three new spoligotypes were reported SB02703, SB02704 and SB02705. We observed 37 MIRU-VNTR patterns, 107 isolates were grouped in 12 clusters and 25 isolates were unique. Spoligotypes SB0121, SB0673, SB0140, SB0145 and SB0120 showed marked subdivision applying MIRU-VNTR method; meanwhile, spoligotypes SB0971 and SB0327 showed single MIRU-VNTR profiles. The Hunter-Gaston discriminatory index (HGDI) was 0.88, 0.78 and 0.90 for 9-loci MIRU-VNTR, spoligotyping and both methods, respectively. Additionally, allelic diversity (h) analysis showed high diversity for QUB3232, QUB26 and QUB11b with h = 0.79, 0.66 and 0.63, respectively. Overall, high genetic variability was observed among M. bovis isolates. Thus, the use of 9-loci MIRU-VNTR panel is enough to describe genetic diversity, evolution and distribution of M. bovis. This study supports the use of these tools for subsequent epidemiological studies in high incidence areas.
  • Genomic Epidemiology of Salmonella enterica Circulating in Surface Waters Used in Agriculture and Aquaculture in Central Mexico
    N. E. Ballesteros-Nova, S. Sánchez, J. L. Steffani, L. C. Sierra, Z. Chen, F. A. Ruíz-López, R. L. Bell, E. A. Reed, M. Balkey, M. S. Rubio-Lozano, O. Soberanis-Ramos, F. Barona-Gómez, E. W. Brown, M. W. Allard, J. Meng, E. J. Delgado-Suárez
    Applied and Environmental Microbiology, 2022
    Surface waters are heavily used in food production worldwide. Several human pathogens can survive in these waters for long periods and disseminate to food production environments, contaminating our food supply.
  • Community Engagement in the Diagnosis and Control of a Bovine Paralytic Rabies Outbreak in Two Rural Communities of Mexico
    Miguel Galarde-López, Gerardo F. Quiroz-Rocha, Irma E. Candanosa-Aranda, Orbelín Soberanis-Ramos, Lourdes García-García
    Journal of Agromedicine, 2022
    Rabies is a neglected zoonosis with adverse public health effects. We describe the community engagement in containing a bovine paralytic rabies outbreak in two rural communities of Mexico. We carried out a participatory rapid appraisal using different community participation techniques for 2 weeks, including information sharing, community meetings, prioritization of activities, and training. In addition to the animal census and vaccination, necropsy and immunofluorescence tests were performed to diagnose rabies. Cattle mortality during the outbreak of bovine paralytic rabies was 4.5% (15/331); 1446 anti-rebies vacines were applied, directly benefiting 94 families. Members of two rural communities were trained. The continuous exchange of information among the stakeholders allowed us to inform, consult, involve, and empower community members. Community participation played an essential role in identifying a common problem, implementing activities to contain it, and successfully safeguarding public health, animal production, and food security.
  • Measurement of the physicochemical, performance, and consumer-information characteristics of commercial nonmedicated shampoo for dogs
    S.I. Peña-Corona, I. Juárez-Rodríguez, D. Vargas-Estrada, E. Amador-González, L. Arvizu-Tovar, O. Soberanis-Ramos
    Polish Journal of Veterinary Sciences, 2022
    The aim was to analyze the quality of commercial shampoo without prophylactic effect for dogs. The analysis was based on the evaluations and requirements established for human-line products since there is no guide for veterinary products in Mexico; such evaluations have not been carried out or published in Mexico. Physicochemical, sensory, performance, and consumer information tests of the shampoo were carried out. The sample consisted of twenty products marketed in Mexico City. During the evaluation of the label, a serious non-compliance with applicable regulations was found. The pH of the products ranged between 5.6 and 8.4; Significant differences (p⟨0.05) were found between the three groups with low (6.1), medium (7.2), and high (8.1) pH. Viscosity values were from 1131 to 3102. For the foam index, no statistically significant differences were found. 100% of the products analyzed complied with the rest of the quality tests carried out. The results of the quality analysis in this study will allow veterinarians specializing in small species to better select and recommend the products for their use and inform dog owners, about the safety, and value of the products.
  • High exposure to pathogenic leptospires by the population residing in dairy farms in Hidalgo, Mexico
    Miguel Galarde-López, Miriam Bobadilla-del Valle, Luisa María Sánchez-Zamorano, Anabel Ordaz-Vázquez, Maria Elena Velazquez-Meza, Orbelín Soberanis-Ramos
    Brazilian Journal of Microbiology, 2021
  • Genomic surveillance of antimicrobial resistance shows cattle and poultry are a moderate source of multi-drug resistant nontyphoidal Salmonella in Mexico
    Enrique Jesús Delgado-Suárez, Tania Palós-Guitérrez, Francisco Alejandro Ruíz-López, Cindy Fabiola Hernández Pérez, Nayarit Emérita Ballesteros-Nova, Orbelín Soberanis-Ramos, Rubén Danilo Méndez-Medina, Marc W. Allard, María Salud Rubio-Lozano
    Plos One, 2021
  • Responsible dog ownership in Mexico City
    Orbelín Soberanis-Ramos, Laura O Arvizu-Tovar, Juan José Pérez-Rivero
    Salud Publica De Mexico, 2018
  • Seroprevalence of brucellosis among dairy farm workers in Mexico
    Miguel E Cervera-Hernández, Anabel Ordaz-Vázquez, Pedro Torres-González, Bárbara Chávez-Mazar, Orbelin Soberanis-Ramos, José Sifuentes-Osornio, Alfredo Ponce de León, Miriam Bobadilla-del Valle
    Salud Publica De Mexico, 2016
  • Prevalence of Latent and Active Tuberculosis among Dairy Farm Workers Exposed to Cattle Infected by Mycobacterium bovis
    Pedro Torres-Gonzalez, Orbelin Soberanis-Ramos, Areli Martinez-Gamboa, Barbara Chavez-Mazari, Ma Teresa Barrios-Herrera, Martha Torres-Rojas, Luis Pablo Cruz-Hervert, Lourdes Garcia-Garcia, Mahavir Singh, Adrian Gonzalez-Aguirre, Alfredo Ponce de Leon-Garduño, José Sifuentes-Osornio, Miriam Bobadilla-del-Valle
    Plos Neglected Tropical Diseases, 2013