Marcelo Henrique Santos Paiva

Scopus Publications

Control and mitigation of dengue and Zika virus transmission in a hospital in Recife, Brazil: a successful experience with an integrated control program against Aedes aegypti
Helena Emanuela Candida-Silva, Henrique Rafael Pontes Ferreira, Jaziela de Arruda Mendonça, Rafael Alves da Silva, Larissa Krokovsky, Letícia de Oliveira Martins, Kathyanne Ellen da Silva Barbosa, Frederico Jorge Ribeiro, Cláudia Maria Fontes de Oliveira, Constância Flávia Junqueira Ayres, Marcelo Henrique Santos Paiva, Maria Alice Varjal de Melo-Santos
Parasites and Vectors, 2026
Background The presence of Aedes aegypti in healthcare facilities represents a significant epidemiological threat, as it is the primary vector of dengue (DENV), Zika (ZIKV), and chikungunya (CHIKV) viruses. In the absence of effective alternatives to chemical insecticides for eliminating adult mosquitoes, healthcare facilities may become focal points for arbovirus transmission, compromising the safety of patients and healthcare employees. Vector control remains the main approach for mitigating arbovirus transmission. This study investigated the impact of an effective integrated control program (ICP) implemented in a hospital in Recife, Pernambuco, Brazil, where arbovirus circulation in mosquitoes was detected. Methods Entomological monitoring was conducted through mechanical aspiration inside the Hospital das Clínicas (HC). Specimens were counted, identified, grouped by species, classified according to blood-feeding status, and recorded by capture station. Viral infection was assessed using nonstructural protein 1 (NS1) by enzyme-linked immunosorbent assay (ELISA) and a triplex reverse transcription quantitative polymerase chain reaction (RT-qPCR) for the detection of DENV, ZIKV, and CHIKV. Infection rates were calculated using the minimum infection rate (MIR). ICP actions included environmental management, larvicide application at breeding sites, ovitraps to collect and destroy eggs, toxic sugar baits, and intensified mechanical aspiration to eliminate adult mosquitoes. Three phases were defined: pre-ICP (initial survey), ICP (all actions), and post-ICP (larvicidal treatment). Results During the ICP, a sustained reduction in female mosquito density was observed, reaching 99% for Ae. aegypti and 88% for Culex quinquefasciatus . Six months after ICP, ELISA detected infected females in 9 of 14 stations (64%). The MIR for Ae. aegypti decreased from 50 to 44, while Cx. quinquefasciatus showed a slight increase. RT-qPCR revealed 11 ZIKV-positive pools (eight Ae. aegypti and three Cx. quinquefasciatus ). After 12 months of ICP, only one Ae. aegypti DENV-positive pool was detected by both techniques in a single station (pediatrics). Both techniques detected infection even in pools containing a single female, underscoring their high sensitivity. At 18 months of ICP, all pools tested negative for arboviruses. Conclusions Molecular and antigen-based approaches confirmed the mitigation of arbovirus circulation in hospital-associated mosquitoes, demonstrating that the continuous elimination of Ae. aegypti females (> 99%) is critical in these settings to prevent nosocomial transmission. Graphical abstract
Genomic epidemiology of dengue virus serotype 3 lineage 3III_B.3.2 in Brazil: insights into its spread and dengue severity amid co-circulation of multiple dengue serotypes
Caroline do Nascimento Ferreira, Carla Adriane Royer, Camila Confortin, Nelson Fernando Quallio Marques, Mayra Marinho Presibella, Aline Andrade Freund, Karoline Rodrigues Campos, Claudio Tavares Sacchi, Adriano Abbud, Gustavo Barbosa de Lima, Clarice Neuenschwander Lins de Morais, Veronica Gomes da Silva, Keilla Maria Paz e Silva, Mayara Matias de Oliveira Marques da Costa, Sabrina Gonçalves, Sofia Isabel Rótulo Araújo, Darcita Buerger Rovaris, Edson Delatorre, Rodrigo Ribeiro-Rodrigues, Pedro Augusto Alves, Daisy Maria Strottmann, Camila Zanluca, Claudia Nunes Duarte dos Santos, Felipe Gomes Naveca, Gabriel da Luz Wallau, Daniel Maximo Correa Alcantara, Zoraida del Carmen Fernandez Grillo, Hellen Geremias Gatica Santos, Gonzalo Bello, Tiago Gräf, Suzana Harue Umeo, Juan Gabriel Carvalheiro de Araújo, Lavinia Nery Villa Stangler Arend, Celia Fagundes da Cruz, Claudia Regina Gonçalves, Fernanda Modesto Tolentino Binharde, Adriana Yurika Maeda, Camila Maria dos Santos, Camila Aoyama Vieira, Gislene Garcia de Castro Lichs, Luiz Henrique Ferraz Demarchi, Marina Castilhos Souza Umaki Zardin, Alexandre Freitas da Silva, Filipe Zimmer Dezordi, Marcelo Henrique Santos Paiva, Matheus Filgueira Bezerra, Bartolomeu Acioli-Santos, Clintiano Curvêlo da Silva, Elisa de Almeida Neves Azevedo, Tulio de Lima Campos, Andreza Pâmela Vasconcelos, Diego Arruda Falcão, Senele Ana de Alcantara Belettini, Vanessa Pecini da Cunha, Mariza Hoffmann Machado, Marcio Pacheco de Andrade, Felipe Donateli Gatti, Gabriela Mendonça de Colombo, Joana Zorzal Nodari, Thiago de Jesus Sousa, Ana Paula Moreira Franco Luiz, Thaís Bárbara de Souza Silva
Lancet Regional Health Americas, 2026
<h2>Summary</h2><h3>Background</h3> After over a decade of minimal circulation, DENV-3 reemerged in Brazil in 2023, driven by the newly detected lineage 3III_B.3.2. Elucidating the patterns of DENV-3 spread and impact on public health, is critically important in the context of Brazil's hyperendemic dengue scenario. Here, we aimed to investigate the spatiotemporal dispersion of this new lineage and its epidemiological impact in Brazil. <h3>Methods</h3> We generated 87 near-complete DENV-3 genomes, sampled in nine different Brazilian states, that were analysed with publicly available sequences. Phylogeographic reconstructions inferred introduction events and spatial dispersion of lineage 3III_B.3.2. In parallel, dengue cases notifications (2023–2025) were used to fit multiple logistic regression models assessing associations between DENV serotypes and severity, adjusting for demographic and spatiotemporal covariates. <h3>Findings</h3> We detected at least 14 introductions of lineage 3III_B.3.2 into Brazil during 2023–2024, followed by diversification into six region-specific clades. Epidemiological analyses applied to the RT-qPCR dengue confirmed cases showed an adjusted odds ratios for severe dengue ranging from 1.24 to 1.71 for DENV-2 and from 1.47 to 1.94 for DENV-3, across different settings and using DENV-1 infection as the reference category. Sensitivity analysis to evaluate potential recent heterotypic infection among infants ≤2 years adjusted by confunders showed an association between DENV-2 and severe dengue, while DENV-3 estimates were not statistically significant. <h3>Interpretation</h3> Our results reveal a dynamic pattern of multiple introductions and regionally structured spread of 3III_B.3.2, which may represent a general feature as a similar reconstructed history has also been observed for other DENV serotypes/lineages in Brazil. Although the association observed between DENV-2 and DENV-3 and dengue severity may be influenced by secondary infections, our findings underscore that the co-circulation of multiple serotypes contributes to more severe epidemic outcomes and emphasize the need for ongoing DENV genomic surveillance. <h3>Funding</h3> DECIT, CGLAB, Fiocruz, CNPq, and FAPES.
International multisite implementation of distributed cell-free protein biomanufacturing to advance health and research equity
Severino Jefferson Ribeiro da Silva, Quinn Matthews, Séverine Cazaux, Justin R. J. Vigar, Bárbara N. R. Santos, Deyse C. M. Carvalho, Lauren A. Cranmer, David Duplat, Serena Singh, Mohammad Simchi, Paula Benítez-Bolivar, Thaíse Yasmine Vasconcelos de Lima Cavalcanti, Kaiyue Wu, Renata P. G. Mendes, Tanvi Kale, Ana L. L. Divarzak, Kristof Bozovicar, Jennifer Doucet, Anibal Arce, Cielo Léon, Valentina Ferrando, Jessica Nguyen, Anson Ho, Suelen C. de Lima, Pouriya Bayat, Yuxiu Guo, Seray Cicek, Aidan Tinafar, Larissa Krokovsky, Laís C. Machado, Ashyad Rayhan, Idorenyin A. Iwe, Alexander Klenov, Thiago P. G. de Araujo, Jean P. M. Nascimento, Jurandy J. F. Magalhães, Marcela Guevara-Suarez, Patricia Garcia Canete, Kodjo Ayi, Moiz Charania, Tara E. Stehelin, Kate Chatfield-Reed, Marcelo H. S. Paiva, Ian Crandall, Tony Mazzulli, Gabriel da Luz Wallau, Abelardo Silva-Júnior, Adriana Bernal, Chaitanya A. Athale, Alexander A. Green, Scott C. Weaver, Camila González, Fernán Federici, Lindomar Pena, Keith Pardee
Science Advances, 2026
Limitations in global access to research and health care capacity undermine equity, sustainability, and resilience, particularly in resource-limited settings. Molecular diagnostics and biologic therapeutics are set to revolutionize medicine; however, our dependence on centralized biomanufacturing and cold chain logistics restricts access to these benefits. These constraints reflect a chronic and unmet global challenge. Here, with research teams in North and South America and Asia, we challenge the top-down paradigm by advancing community-driven solutions that empower underserved populations to participate in the bioeconomy, producing what they need, when, and where they need it. Our approach leverages low-burden biomanufacturing—built on cell-free protein synthesis and open-source hardware—to enable local, on-demand production of high-value bioproducts, including growth factors, vaccines, and diagnostic enzymes, demonstrating performance comparable to commercial gold standards. Implemented at 10 sites worldwide, this platform supported patient trials targeting globally relevant pathogens. Together, these efforts lay the foundation for a globally inclusive biomanufacturing ecosystem, where innovation goes beyond geographic boundaries.
Oropouche virus outbreaks in northeast Brazil between 2024-25 are characterized by sustained transmission and spread to newly affected areas
Elverson Soares de Melo, Sophia Maria Dantas da Silva, Gustavo Barbosa de Lima, Adalúcia da Silva, Alexandre Freitas da Silva, Verônica Gomes da Silva, Elisa de Almeida Neves Azevedo, Letícia Welter Rother, Keilla Maria Paz e Silva, Diego Arruda Falcão, Andreza Pâmela Vasconcelos, Mayara Matias de Oliveira Marques da Costa, Eduardo Augusto Duque Bezerra, Thiago Franco de Oliveira Carneiro, Erik Matthaus de Lima Paiva, Janaina Correia Oliveira, Matheus Filgueira Bezerra, Marcelo Henrique Santos Paiva, Bartolomeu Acioli-Santos, Clarice Neuenschwander Lins de Morais, Tulio de Lima Campos, Gabriel da Luz Wallau
Plos Neglected Tropical Diseases, 2026
Oropouche virus (OROV) has recently expanded in Brazil, establishing transmission in non-endemic regions. This study aims to integrate epidemiological and molecular data to investigate OROV spread in Northeast (NE) Brazil between 2024 and 2025. OROV cases were analyzed regarding ecological risk factors and geographical clustering. Additionally, we sequenced 65 new OROV genomes from the Northeast states of Pernambuco, Paraíba, and Sergipe to infer the virus’s spatiotemporal dynamics in NE Brazil. A total of 2,806 confirmed cases were reported between March 2024 and April 2025, affecting 170 municipalities across eight out of nine NE states, with highly heterogeneous incidence. An ecological shift was observed, with OROV transmission moving from Atlantic Forest areas in 2024 to humid Caatinga zones in 2025. Phylogenetic reconstruction revealed multiple independent viral introductions in Northeast in 2024, including two in Pernambuco. The first, originating from the central Amazonas, became the main driver of local transmission and subsequently spread to Sergipe and Paraíba, causing outbreaks in 2024 and 2025, respectively. The second introduction remained restricted within Pernambuco. While several Northeast municipalities reported high OROV incidence, Jaqueira (Pernambuco) emerged as a key hub for regional viral spread. OROV showed sustained transmission in the region over a two-year period, characterized by marked spatiotemporal displacement consistent with short-lived, rapidly spreading outbreaks, followed by cryptic transmission and subsequent dissemination to new areas, ultimately driving renewed intense outbreaks.
Horizons of the Future: Preparedness and Response
Luísa Maria Inácio da Silva, Rafael Maciel-de-Freitas, Marcelo Henrique Santos Paiva, Gabriel da Luz Wallau
Current Topics in Microbiology and Immunology, 2026
Dissecting Zika virus transmission by Aedes aegypti and Culex quinquefasciatus mosquitoes to a vertebrate host and its coinfection with Mayaro virus
Larissa Krokovsky, Carlos Ralph Batista Lins, Duschinka Ribeiro Duarte Guedes, Gabriel da Luz Wallau, Constância Flávia Junqueira Ayres, Marcelo Henrique Santos Paiva
Frontiers in Microbiology, 2026
In recent years, the co-circulation of arboviruses including Zika (ZIKV), dengue (DENV), and chikungunya (CHIKV) has been increasingly reported worldwide, contributing to a complex and poorly understood epidemiological scenario. The simultaneous transmission of multiple arboviruses through a single mosquito bite is a documented phenomenon that can influence disease pathogenesis and modify transmission dynamics. This study investigated the dynamics of ZIKV infection in Aedes aegypti (RecLab) and Culex quinquefasciatus (CqSLab), and assessed the potential for ZIKV and Mayaro virus (MAYV) co-infection and co-transmission by Ae. aegypti . Mosquitoes were artificially blood-fed with ZIKV, MAYV, or both, and allowed to feed on immunodeficient mice. Clinical signs were monitored in mice, and infection was assessed using RT-qPCR. Finally, to complete the transmission cycle evaluation study, naive mosquitoes were subsequently fed on infected mice. Among 12 mice exposed to ZIKV-infected Ae. aegypti , all developed symptoms by days 7–8 post-exposure. Notably, mice bitten by co-infected Ae. aegypti displayed symptoms as early as days 2–3 post-exposure. Mosquitoes feeding on these mice showed high ZIKV infection rates, which were further elevated in the context of co-infection. Regarding the 12 mice exposed to Cx. quinquefasciatus , they showed no signs of infection, despite RT-qPCR positivity. These results validate a standardized murine model for arbovirus studies and reveal enhanced ZIKV transmission potential during co-infection with MAYV. The findings offer key insights into arbovirus co-infection dynamics and their potential impact on epidemiology and pathogenesis.
Evaluation of the Impact of Coinfection and Superinfection on Chikungunya and Mayaro Viruses’ Replication in Aedes aegypti
Maria Eduarda dos Santos Pereira de Oliveira, Larissa Krokovsky, Maria Júlia Brito Couto, Duschinka Ribeiro Duarte Guedes, George Tadeu Nunes Diniz, Constância Flávia Junqueira Ayres, Marcelo Henrique Santos Paiva
Microorganisms, 2025
The simultaneous circulation of multiple arboviruses, often driven by (re)emergence events, poses challenges to public health systems. In Brazil, the co-circulation of Dengue virus (DENV), Zika virus (ZIKV), Chikungunya virus (CHIKV), and Oropouche virus (OROV), together with the potential urban emergence of Mayaro virus (MAYV), underscores the importance of understanding interactions among these pathogens within their vectors. This study investigated the effects of CHIKV and MAYV coinfection and superinfection on replication dynamics in Aedes aegypti. Mosquitoes were experimentally exposed to CHIKV and MAYV through artificial blood meals under coinfection and superinfection conditions. Infection (IR), dissemination (DR), and transmission (TR) rates, as well as viral loads, were quantified by quantitative reverse transcription PCR (qRT-PCR). To confirm viral replication and assess cytopathic effects, positive saliva samples were inoculated in Vero cells, followed by serial passages and plaque assays for viral titration. The results showed that Ae. aegypti is capable of transmitting both CHIKV and MAYV concurrently during coinfection. However, in superinfection scenarios, prior infection with either virus significantly reduced the transmission efficiency of the subsequently acquired virus, indicating viral interference at the replication level. These findings underscore the complexity of arboviral interactions within vectors and highlight their potential implications for transmission dynamics. Continuous entomo-virological surveillance and targeted research are essential for anticipating and mitigating the impact of arboviral co-circulation in endemic regions.
Oropouche Virus Importation in Southern Brazil and Emerging Concern Calling for Enhanced Public Health Surveillance
Franciellen Machado dos Santos, Elverson Soares de Melo, Gustavo Barbosa de Lima, Alexandre Freitas da Silva, Marcelo Henrique Santos Paiva, Bartolomeu Acioli‐Santos, Clarice Neuenschwander Lins de Moraes, Amanda Pellenz Ruivo, Tatiana Schäffer Gregianini, Milena Bauermann, Thales Bermann, Valeska Lizzi Lagranha, Ludmila Fiorenzano Baethgen, Fernanda Godinho, Gabriel da Luz Wallau, Ana Beatriz Gorini da Veiga, Richard Steiner Salvato
Journal of Medical Virology, 2025
Oropouche virus (OROV), an arthropod‐borne virus transmitted by Culicoides paraensis , is an endemic arbovirus that historically circulates mostly in the Amazon basin. Between 2022 and 2024, it reemerged as a more widespread public health concern in South America. We conducted a pooled‐sample molecular surveillance study to understand the prevalence of Oropouche fever in Brazil's southernmost state. Over 18 months, we analyzed 4060 samples to monitor the virus emergence in the Rio Grande do Sul state. We detected the first human case of OROV in the state, and our phylogenetic reconstruction indicated a travel‐related introduction from the Amazon region into Rio Grande do Sul. Despite the absence of local transmission, the invasion of Culicoides paraensis and enzootic circulation of the OROV in Rio Grande do Sul highlight the risk of Oropouche fever outbreaks in the region. We demonstrated that pooled‐sample surveillance effectively monitors virus introduction during periods of low endemic circulation, serving as an essential active surveillance tool for the timely detection of virus emergence and enhancing public health preparedness. The multiple introductions of distinct OROV lineages into southern Brazil underscore the importance of genomic surveillance and public health strategies to monitor and mitigate arbovirus spread in the region.
Development of a New Trapping System with Potential Implementation as a Tool for Mosquito-Borne Arbovirus Surveillance
Luísa Maria Inácio da Silva, Larissa Krokovsky, Rafaela Cassiano Matos, Gabriel da Luz Wallau, Marcelo Henrique Santos Paiva
Insects, 2025
Mosquitoes of the Aedes and Culex genera are primary vectors of arboviruses such as the dengue, Zika, chikungunya (CHIKV), Oropouche, and West Nile viruses, causing millions of infections annually. Standard virus detection in mosquitoes requires capturing, transporting, and processing samples with a cold chain to preserve RNA, which is challenging in resource-limited areas. FTA cards preserve viral RNA at room temperature and have been used to collect mosquito saliva, a key sample for assessing transmission. However, most FTA-based traps require electricity or CO2, limiting use in low-resource settings. This study adapted and evaluated the BR-ArboTrap, a low-cost trap derived from an oviposition trap, integrating a sugar-based attractant with FTA cards to collect mosquito saliva, without electricity or refrigeration. Aedes aegypti exposed to CHIKV were used in three experiments to evaluate: (i) RNA preservation under different conditions, (ii) the minimum number of positive mosquitoes for detection, and (iii) RNA amounts on FTA versus blood. RT-qPCR detected CHIKV RNA in 90% of FTA cards and 96% of exposed mosquitoes. RNA remained stable under varying conditions, with no significant difference compared to blood. BR-ArboTrap is an effective, affordable, and field-ready tool to enhance arbovirus surveillance in remote and low-resource areas.
Diversity and Spatial Distribution of Mosquitoes (Culicidae) in Forest-Urban Transition Areas of an Atlantic Forest Fragment in Pernambuco, Brazil
ROSÂNGELA MARIA R. BARBOSA, ELILDE MIGUEL DE SOUSA, GABRIEL B. FAIERSTEIN, CARLOS F. LUNA, ANDRÉ LUIZ S. DE OLIVEIRA, MARCELO HENRIQUE S. PAIVA
Anais Da Academia Brasileira De Ciencias, 2025
The world is experiencing a biodiversity crisis as numerous regions witness massive deforestation in tropical ecosystems. This phenomenon is particularly acute in the Atlantic Forest. Forest edges, where humans, mosquitoes, and wildlife interact, can serve as a bridge for the exchange of zoonotic arboviruses. We aimed to identify mosquito species from an Atlantic Forest fragment in Pernambuco, Northeast Brazil, at the forest edge and in an area with artificial constructions. Mosquito collections were carried out biweekly from February to December 2016. Adult mosquitoes were collected using CDC traps and aspirators, while immature were collected using ovitraps and BR-OVT traps. A total of 8,972 eggs were captured by ovitraps and 168 rafts by BR-OVT traps. A total of 474 adult mosquitoes were collected by CDC traps and 268 by aspirators. Twenty-six different species were identified, all from the subfamily Culicinae, distributed in eight genera (Aedes, Aedeomyia, Culex, Coquillettidia, Limatus, Mansonia, Uranotaenia and Wyeomyia). The most abundant mosquito species in the collections were Cx. quinquefasciatus (69.8%) and Ae. albopictus (22.3%), two species linked to the transmission of arboviruses. Early identification of vector mosquitoes in remaining areas of Atlantic Forest with increasing urban development should be continuous, with a monitoring strategy to increase the effectiveness of control programs.
Higher frequency of interstate over international transmission chains of SARS-CoV-2 virus at the Rio Grande do Sul - Brazil state borders
Filipe Zimmer Dezordi, José Valter Joaquim Silva Júnior, Terimar Facin Ruoso, Angela Giovana Batista, Pedro Mesquita Fonseca, Larissa Paim Bernardo, Richard Steiner Salvato, Tatiana Schäffer Gregianini, Thaísa Regina Rocha Lopes, Eduardo Furtado Flores, Rudi Weiblen, Patrícia Chaves Brites, Mônica de Medeiros Silva, João Batista Teixeira da Rocha, Gustavo de Lima Barbosa, Lais Ceschini Machado, Alexandre Freitas da Silva, Marcelo Henrique Santos Paiva, Matheus Filgueira Bezerra, Tulio de Lima Campos, Tiago Gräf, Daniel Angelo Sganzerla Graichen, Elgion Lucio da Silva Loreto, Gabriel da Luz Wallau
Virus Research, 2025
Sequencing and Analysis of Wolbachia Strains from A and B Supergroups Detected in Sylvatic Mosquitoes from Brazil
Luísa Maria Inácio da Silva, José Irnaldo da Silva, Alexandre Freitas da Silva, Filipe Zimmer Dezordi, Lais Ceschini Machado, Si Qin, Hang Fan, Yigang Tong, Túlio de Lima Campos, Marcelo Henrique Santos Paiva, Gabriel Luz Wallau
Microorganisms, 2024
Genomic and phenotypic characterization of the Oropouche virus strain implicated in the 2022–24 large-scale outbreak in Brazil
Elisa de A. N. Azevedo, Alexandre F. da Silva, Verônica G. da Silva, Lais C. Machado, Gustavo B. de Lima, Bruno I. M. Ishigami, Keilla M. Paz e Silva, Mayara M. de O. M. da Costa, Diego A. Falcão, Andreza P. Vasconcelos, Clintiano C. da Silva, Felipe G. Naveca, Matheus F. Bezerra, Tulio de L. Campos, Bartolomeu Acioli‐Santos, Marcelo H. S. Paiva, Clarice N. L. de Morais, Gabriel L. Wallau
Journal of Medical Virology, 2024
Spatiotemporal transmission of SARS-CoV-2 lineages during 2020-2021 in Pernambuco—Brazil
Lais Ceschini Machado, Filipe Zimmer Dezordi, Gustavo Barbosa de Lima, Raul Emídio de Lima, Lilian Caroliny Amorim Silva, Leandro de Mattos Pereira, Alexandre Freitas da Silva, Antonio Marinho da Silva Neto, André Luiz Sá de Oliveira, Anderson da Costa Armstrong, Rômulo Pessoa-e-Silva, Rodrigo Moraes Loyo, Barbara de Oliveira Silva, Anderson Rodrigues de Almeida, Maira Galdino da Rocha Pitta, Francisco de Assis da Silva Santos, Marilda Mendonça Siqueira, Paola Cristina Resende, Edson Delatorre, Felipe Gomes Naveca, Fabio Miyajima, Tiago Gräf, Rodrigo Feliciano do Carmo, Michelly Cristiny Pereira, Tulio de Lima Campos, Matheus Filgueira Bezerra, Marcelo Henrique Santos Paiva, Gabriel da Luz Wallau, and
Microbiology Spectrum, 2024
Exposure to Zika and chikungunya viruses impacts aspects of the vectorial capacity of Aedes aegypti and Culex quinquefasciatus
Mônica Crespo, Duschinka Guedes, Marcelo Paiva, Mariana Sobral, Elisama Helvecio, Rafael Alves, George Tadeu, Claudia Oliveira, Maria Alice Varjal Melo-Santos, Rosângela Barbosa, Constância Ayres
Plos One, 2024
Integrated Strategies for Aedes aegypti Control Applied to Individual Houses: An Approach to Mitigate Vectorial Arbovirus Transmission
Danielle Cristina Tenório Varjal de Melo, Eloína Maria de Mendonça Santos, Morgana Nascimento Xavier, Josimara do Nascimento, Victor Araújo Barbosa, André Luiz de Sá Oliveira, Marcos Vinícius Meiado, Maria Alice Varjal de Melo-Santos, Marcelo Henrique Santos Paiva, Gabriel da Luz Wallau, Cláudia Maria Fontes de Oliveira
Tropical Medicine and Infectious Disease, 2024
Assessment of clinical characteristics and viral load in individuals infected by Delta and Omicron variants of SARS-CoV-2
Sávio Luiz Pereira Nunes, Chirles Araújo de França, Gabriela Dias Rocha, Samily Aquino de Sá Oliveira, Mariana Ramos Freitas, Eliane Oliveira da Silva, Katia Sampaio Coutinho, Aline Silva Jerônimo, Gustavo Barbosa de Lima, Raul Emídio de Lima, Matheus Filgueira Bezerra, Filipe Zimmer Dezordi, Marcelo Henrique Santos Paiva, Gabriel da Luz Wallau, Carlos Dornels Freire de Souza, Anderson da Costa Armstrong, Rodrigo Feliciano do Carmo
Heliyon, 2023
Multiple introductions and country-wide spread of DENV-2 genotype II (Cosmopolitan) in Brazil
Tiago Gräf, Caroline Do Nascimento Ferreira, Gustavo Barbosa de Lima, Raul Emídio de Lima, Lais Ceschini Machado, Tulio de Lima Campos, Michelle Orane Schemberger, Helisson Faoro, Marcelo Henrique Santos Paiva, Matheus Filgueira Bezerra, Valdinete Nascimento, Victor Souza, Fernanda Nascimento, Matilde Mejía, Dejanane Silva, Yasmin Silva de Oliveira, Luciana Gonçalves, Tatyana Costa Amorim Ramos, Daniel Barros de Castro, Ana Ruth Arcanjo, Herton Augusto Pinheiro Dantas, Mayra Marinho Presibella, Sandra Bianchini Fernandes, Tatiana Schaffer Gregianini, Keilla Maria Paz E Silva, Claudio Tavares Sacchi, Ana Cecília Ribeiro Cruz, Claudia Nunes Duarte dos Santos, Ana Maria Bispo de Filippis, Gonzalo Bello, Gabriel Luz Wallau, Richard Steiner Salvato, Felipe Naveca
Virus Evolution, 2023
Dynamic of Mayaro Virus Transmission in Aedes aegypti, Culex quinquefasciatus Mosquitoes, and a Mice Model
Larissa Krokovsky, Carlos Ralph Batista Lins, Duschinka Ribeiro Duarte Guedes, Gabriel da Luz Wallau, Constância Flávia Junqueira Ayres, Marcelo Henrique Santos Paiva
Viruses, 2023
Effects of gamma radiation on the vector competence of Aedes aegypti (diptera: Culicidae) to transmit Zika virus
Edvane Borges da Silva, Carlos Messias de Mendonça, Duschinka Ribeiro Duarte Guedes, Marcelo Henrique Santos Paiva, Jaziela de Arruda Mendonça, Elisângela Santos Ferreira Dias, Sloana Giesta Lemos Florêncio, Ademir Amaral, André Maciel Netto, Constância Flávia Junqueira Ayres Lopes, Maria Alice Varjal de Melo-Santos
Acta Tropica, 2023
Real-life evaluation of a rapid antigen test (DPP SARS-CoV-2 Antigen) for COVID-19 diagnosis of primary healthcare patients, in the context of the Omicron-dominant wave in Brazil
Matheus Filgueira Bezerra, Lilian Caroliny Amorim Silva, Rômulo Pessoa-e-Silva, Gisele Lino Soares, Filipe Zimmer Dezordi, Gustavo Barbosa Lima, Raul Emídio Lima, Tulio L. Campos, Cassia Docena, Anderson Bruno Oliveira, Maira Galdino da Rocha Pitta, Francisco de Assis da Silva Santos, Michelly Pereira, Gabriel Luz Wallau, Marcelo Henrique Santos Paiva
Clinical Microbiology and Infection, 2023
Chikungunya virus infection in the southernmost state of Brazil was characterised by self-limited transmission (2017-2019) and a larger 2021 outbreak
Memorias do Instituto Oswaldo Cruz, 2023
Potential Nosocomial Infections by the Zika and Chikungunya Viruses in Public Health Facilities in the Metropolitan Area of Recife, Brazil
Larissa Krokovsky, Duschinka Ribeiro Duarte Guedes, Fabiana Cristina Fulco Santos, Kamila Gaudêncio da Silva Sales, Daniela Anastácio Bandeira, Claudenice Ramos Pontes, Walter Soares Leal, Constância Flávia Junqueira Ayres, Marcelo Henrique Santos Paiva
Tropical Medicine and Infectious Disease, 2022
Phylogenetic-based inference reveals distinct transmission dynamics of SARS-CoV-2 lineages Gamma and P.2 in Brazil
Tiago Gräf, Gonzalo Bello, Felipe Gomes Naveca, Marcelo Gomes, Vanessa Leiko Oikawa Cardoso, Alexandre Freitas da Silva, Filipe Zimmer Dezordi, Mirleide Cordeiro dos Santos, Katia Correa de Oliveira Santos, Érika Lopes Rocha Batista, Alessandro Leonardo Álvares Magalhães, Fernando Vinhal, Fábio Miyajima, Helisson Faoro, Ricardo Khouri, Gabriel Luz Wallau, Edson Delatorre, Marilda Mendonça Siqueira, Paola Cristina Resende, Tirza Peixoto Mattos, Valdinete Alves Nascimento, Victor Souza, André de Lima Guerra Corado, Fernanda Nascimento, George Silva, Matilde Mejía, Maria Júlia Brandão, Ágatha Costa, Karina Pessoa, Michele Jesus, Luciana Fé Gonçalves, Cristiano Fernandes, Valnete Andrade, Luana Barbagelata, Ana Cecília Ribeiro Cruz, Andrea Costa, Lindomar dos Anjos Silva, Jucimária Dantas Galvão, Anderson Brandao Leite, Felicidade Mota Pereira, Thais Oliveira Costa, Joaquim Cesar Sousa, Lidio Gonçalves Lima Neto, Haline Barroso, Dalane Loudal Florentino Teixeira, Joao Felipe Bezerra, Cássia Docena, Raul Emídio de Lima, Lilian Caroliny Amorim Silva, Gustavo Barbosa de Lima, Laís Ceschini Machado, Matheus Filgueira Bezerra, Marcelo Henrique Santos Paiva, Maria Eduarda Pessoa Lopes Dantas, Raíssa Liane Do Nascimento Pereira, Josélio Araújo, Cliomar A. Santos, Rodrigo Ribeiro-Rodrigues, André Felipe Leal Bernardes, Felipe Campos de Melo Iani, Beatriz Grinsztejn, Valdiléa G. Veloso, Patricia Brasil, Anna Carolina Dias da Paixão, Luciana Reis Appolinario, Renata Serrano Lopes, Fernando do Couto Motta, Alice Sampaio Rocha, Taina Moreira Martins Venas, Elisa Cavalcante Pereira, Andrea Cony Cavalcanti, Leonardo Soares Bastos, Luis Fernando de Macedo Brigido, Mauro de Medeiros Oliveira, Michelle Orane Schemberger, Andreia Akemi Suzukawa, Irina Riediger, Maria do Carmo Debur, Richard Steiner Salvato, Tatiana Schäffer Gregianini, Darcita Buerger Rovaris, Sandra Bianchini Fernandes
Iscience, 2022
Effects of gamma radiation on the reproductive viability of Aedes aegypti and its descendants (Diptera: Culicidae)
Edvane Borges Silva, Carlos Messias Mendonça, Jaziela de Arruda Mendonça, Elisângela Santos Ferreira Dias, Sloana Giesta Lemos Florêncio, Duschinka Ribeiro Duarte Guedes, Marcelo Henrique Santos Paiva, Ademir Amaral, André Maciel Netto, Maria Alice Varjal Melo-Santos
Acta Tropica, 2022
Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples
Margot Karlikow, Severino Jefferson Ribeiro da Silva, Yuxiu Guo, Seray Cicek, Larissa Krokovsky, Paige Homme, Yilin Xiong, Talia Xu, Maria-Angelica Calderón-Peláez, Sigrid Camacho-Ortega, Duo Ma, Jurandy Júnior Ferraz de Magalhães, Bárbara Nayane Rosário Fernandes Souza, Diego Guerra de Albuquerque Cabral, Katariina Jaenes, Polina Sutyrina, Tom Ferrante, Andrea Denisse Benitez, Victoria Nipaz, Patricio Ponce, Darius G. Rackus, James J. Collins, Marcelo Paiva, Jaime E. Castellanos, Varsovia Cevallos, Alexander A. Green, Constância Ayres, Lindomar Pena, Keith Pardee
Nature Biomedical Engineering, 2022
Arbovirus Surveillance in Field-Collected Mosquitoes From Pernambuco-Brazil, During the Triple Dengue, Zika and Chikungunya Outbreak of 2015-2017
Larissa Krokovsky, Marcelo H. S. Paiva, Duschinka R. D. Guedes, Rosângela M. R. Barbosa, André L. S. de Oliveira, Daniela B. Anastácio, Claudenice Ramos Pontes, Constância F. J. Ayres
Frontiers in Tropical Diseases, 2022
Unusual SARS-CoV-2 intrahost diversity reveals lineage superinfection
Filipe Zimmer Dezordi, Paola Cristina Resende, Felipe Gomes Naveca, Valdinete Alves do Nascimento, Victor Costa de Souza, Anna Carolina Dias Paixão, Luciana Appolinario, Renata Serrano Lopes, Ana Carolina da Fonseca Mendonça, Alice Sampaio Barreto da Rocha, Taina Moreira Martins Venas, Elisa Cavalcante Pereira, Marcelo Henrique Santos Paiva, Cassia Docena, Matheus Filgueira Bezerra, Laís Ceschini Machado, Richard Steiner Salvato, Tatiana Schäffer Gregianini, Leticia Garay Martins, Felicidade Mota Pereira, Darcita Buerger Rovaris, Sandra Bianchini Fernandes, Rodrigo Ribeiro-Rodrigues, Thais Oliveira Costa, Joaquim Cesar Sousa, Fabio Miyajima, Edson Delatorre, Tiago Gräf, Gonzalo Bello, Marilda Mendonça Siqueira, Gabriel Luz Wallau
Microbial Genomics, 2022
First report of the L1014F kdr mutation in wild populations of Anopheles arabiensis in Cabo Verde, West Africa
Derciliano Lopes da Cruz, Marcelo Henrique Santos Paiva, Duschinka Ribeiro Duarte Guedes, Elainne Christine de Souza Gomes, Silvia Gonçalves Pires, Lara Ferrero Gomez, Constância Flávia Junqueira Ayres
Parasites and Vectors, 2021
A Sanger-based approach for scaling up screening of SARS-CoV-2 variants of interest and concern
Matheus Filgueira Bezerra, Lais Ceschini Machado, Viviane do Carmo Vasconcelos De Carvalho, Cássia Docena, Sinval Pinto Brandão-Filho, Constância Flávia Junqueira Ayres, Marcelo Henrique Santos Paiva, Gabriel Luz Wallau
Infection Genetics and Evolution, 2021
Systematic review of wolbachia symbiont detection in mosquitoes: An entangled topic about methodological power and true symbiosis
Luísa Maria Inácio da Silva, Filipe Zimmer Dezordi, Marcelo Henrique Santos Paiva, Gabriel Luz Wallau
Pathogens, 2021
First report of an Onchocercidae worm infecting Psychodopygus carrerai carrerai sandfly, a putative vector of Leishmania braziliensis in the Amazon
Andreia Fernandes Brilhante, Alessandra Lima de Albuquerque, Abraham Cézar de Brito Rocha, Constância Flávia Junqueira Ayres, Marcelo Henrique Santos Paiva, Márcia Moreira de Ávila, Cristiane de Oliveira Cardoso, Isabel L. Mauricio, Eunice Aparecida Bianchi Galati
Scientific Reports, 2020
Outbreak of autochthonous cases of malaria in coastal regions of Northeast Brazil: the diversity and spatial distribution of species of Anopheles
Elainne Christine de Souza Gomes, Derciliano Lopes da Cruz, Maria Alice Varjal Melo Santos, Renata Maria Costa Souza, Cláudia Maria Fontes de Oliveira, Constância Flávia Junqueira Ayres, Renata Martins Domingos, Maria das Graça da Silva Pedro, Marcelo Henrique Santos Paiva, Lílian Maria Lapa Montenegro Pimentel
Parasites and Vectors, 2020
Multiple introductions followed by ongoing community spread of sars-cov-2 at one of the largest metropolitan areas of northeast brazil
Marcelo Henrique Santos Paiva, Duschinka Ribeiro Duarte Guedes, Cássia Docena, Matheus Filgueira Bezerra, Filipe Zimmer Dezordi, Laís Ceschini Machado, Larissa Krokovsky, Elisama Helvecio, Alexandre Freitas da Silva, Luydson Richardson Silva Vasconcelos, Antonio Mauro Rezende, Severino Jefferson Ribeiro da Silva, Kamila Gaudêncio da Silva Sales, Bruna Santos Lima Figueiredo de Sá, Derciliano Lopes da Cruz, Claudio Eduardo Cavalcanti, Armando de Menezes Neto, Caroline Targino Alves da Silva, Renata Pessôa Germano Mendes, Maria Almerice Lopes da Silva, Tiago Gräf, Paola Cristina Resende, Gonzalo Bello, Michelle da Silva Barros, Wheverton Ricardo Correia do Nascimento, Rodrigo Moraes Loyo Arcoverde, Luciane Caroline Albuquerque Bezerra, Sinval Pinto Brandão-Filho, Constância Flávia Junqueira Ayres, Gabriel Luz Wallau
Viruses, 2020
Development of a molecular xenomonitoring protocol to assess filariasis transmission
Alessandra Lima de Albuquerque, Tatiane Alexandre de Araújo, Danielle Cristina Tenório Varjal de Melo, Marcelo Henrique Santos Paiva, Fábio Lopes de Melo, Cláudia Maria Fontes de Oliveira, Constância Flávia Junqueira Ayres
Experimental Parasitology, 2020
Sequencing of ZIKV genomes directly from Ae. aegypti and Cx. quinquefasciatus mosquitoes collected during the 2015–16 epidemics in Recife
Marcelo Henrique Santos Paiva, Duschinka Ribeiro Duarte Guedes, Larissa Krokovsky, Lais Ceschini Machado, Tatiana Maria Teodoro Rezende, Mariana Carolina de Morais Sobral, Constância Flávia Junqueira Ayres, Gabriel Luz Wallau
Infection Genetics and Evolution, 2020
Fast multiplex real-time PCR assay for simultaneous detection of dog and human blood and Leishmania parasites in sand flies
Kamila Gaudêncio da Silva Sales, Débora Elienai de Oliveira Miranda, Marcelo Henrique Santos Paiva, Luciana Aguiar Figueredo, Domenico Otranto, Filipe Dantas-Torres
Parasites and Vectors, 2020
Development and Validation of Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for Rapid Detection of ZIKV in Mosquito Samples from Brazil
Severino Jefferson Ribeiro da Silva, Marcelo Henrique Santos Paiva, Duschinka Ribeiro Duarte Guedes, Larissa Krokovsky, Fábio Lopes de Melo, Maria Almerice Lopes da Silva, Adalúcia da Silva, Constância Flávia Junqueira Ayres, Lindomar J. Pena
Scientific Reports, 2019
An unsettling explanation for the failure of skatole-baited ovitraps to capture Culex mosquitoes
Marcelo H. S. Paiva, Rosângela M. R. Barbosa, Suzane A. Santos, Norma M. Silva, Marcia B. Paula, Constância F. J. Ayres, Walter S. Leal
Insect Science, 2019
Screening Aedes aegypti (Diptera: Culicidae) Populations from Pernambuco, Brazil for Resistance to Temephos, Diflubenzuron, and Cypermethrin and Characterization of Potential Resistance Mechanisms
Ana Paula de Araújo, Marcelo Henrique Santos Paiva, Amanda Maria Cabral, Antônio Emanuel Holanda Dias Cavalcanti, Luiz Fernando Freitas Pessoa, Diego Felipe Araujo Diniz, Elisama Helvecio, Ellyda Vanessa Gomes da Silva, Norma Machado da Silva, Daniela Bandeira Anastácio, Claudenice Pontes, Vânia Nunes, Maria de Fátima Marinho de Souza, Fernando Jorge Rodrigues Magalhães, Maria Alice Varjal de Melo Santos, Constância Flávia Junqueira Ayres
Journal of Insect Science, 2019
Zika virus detection, isolation and genome sequencing through Culicidae sampling during the epidemic in Vitória, Espírito Santo, Brazil
Constância Flavia Junqueira Ayres, Duschinka Ribeiro Duarte Guedes, Marcelo Henrique Santos Paiva, Mariana Carolina Morais-Sobral, Larissa Krokovsky, Laís Ceschini Machado, Maria Alice Varjal Melo-Santos, Mônica Crespo, Cláudia Maria Fontes Oliveira, Ricardo Silva Ribeiro, Orlei Amaral Cardoso, Ana Lúcia Barbosa Menezes, Roberto Costa Laperrière-Jr, Carlos Feitosa Luna, André Luiz Sá Oliveira, Walter Soares Leal, Gabriel Luz Wallau
Parasites and Vectors, 2019
Detection of alleles associated with resistance to chemical insecticide in the malaria vector Anopheles arabiensis in Santiago, Cabo Verde
Derciliano Lopes da Cruz, Marcelo Henrique Santos Paiva, Duschinka Ribeiro Duarte Guedes, Joana Alves, Lara Ferrero Gómez, Constância Flávia Junqueira Ayres
Malaria Journal, 2019
Arbovirus control: what is the (real) stone in the way?
Thaísa Regina Rocha Lopes, Marcelo Henrique Santos Paiva, Pablo Cantalice Santos Farias, José Valter Joaquim Silva Júnior
Revista do Instituto De Medicina Tropical De Sao Paulo, 2019
Arbovirus control: What is the (real) stone in the way?
Thaísa Regina Rocha Lopes, Marcelo Henrique Santos Paiva, Pablo Cantalice Santos Farias, José Valter Joaquim Silva Júnior
Revista do Instituto De Medicina Tropical De Sao Paulo, 2019
Development of an urban molecular xenomonitoring system for lymphatic filariasis in the Recife Metropolitan Region, Brazil
Anita Ramesh, Mary Cameron, Kirstin Spence, Remy Hoek Spaans, Maria A. V. Melo-Santos, Marcelo H. S. Paiva, Duschinka R. D. Guedes, Rosangela M. R. Barbosa, Claudia M. F. Oliveira, André Sá, Claire L. Jeffries, Priscila M. S. Castanha, Paula A. S. Oliveira, Thomas Walker, Neal Alexander, Cynthia Braga
Plos Neglected Tropical Diseases, 2018
Zika virus replication in the mosquito Culex quinquefasciatus in Brazil
Duschinka RD Guedes, Marcelo HS Paiva, Mariana MA Donato, Priscilla P Barbosa, Larissa Krokovsky, Sura W dos S Rocha, Karina LA Saraiva, Mônica M Crespo, Tatiana MT Rezende, Gabriel L Wallau, Rosângela MR Barbosa, Cláudia MF Oliveira, Maria AV Melo-Santos, Lindomar Pena, Marli T Cordeiro, Rafael F de O Franca, André LS de Oliveira, Christina A Peixoto, Walter S Leal, Constância FJ Ayres
Emerging Microbes and Infections, 2017
Circulation of DENV2 and DENV4 in Aedes aegypti (Diptera: Culicidae) mosquitoes from Praia, Santiago Island, Cabo Verde
Duschinka R. D. Guedes, Elisete T. B. Gomes, Marcelo H. S. Paiva, Maria A. V. de Melo-Santos, Joana Alves, Lara F. Gómez, Constância F. J. Ayres
Journal of Insect Science, 2017
Does Zika virus infection affect mosquito response to repellents?
Walter S. Leal, Rosângela M. R. Barbosa, Fangfang Zeng, Gabriel B. Faierstein, Kaiming Tan, Marcelo H. S. Paiva, Duschinka R. D. Guedes, Mônica M. Crespo, Constância F. J. Ayres
Scientific Reports, 2017
Sensitivity of RT-PCR method in samples shown to be positive for zika virus by RT-qPCR in vector competence studies
Marcelo Henrique Santos Paiva, Duschinka Ribeiro Duarte Guedes, Walter Soares Leal, Constância Flávia Junqueira Ayres
Genetics and Molecular Biology, 2017
Histotechnological and socio-epidemiological evaluation of aorta aneurysmal and atheromatous lesions of in humans
International Journal of Clinical and Experimental Pathology, 2017
Response to: ‘Lack of evidence for Zika virus transmission by Culex mosquitoes’
Constância Ayres, Duschinka Guedes, Marcelo Paiva, Mariana Donato, Priscilla Barbosa, Larissa Krokovsky, Sura Rocha, Karina Saraiva, Mônica Crespo, Tatiana Rezende, Gabriel Wallau, Rosângela Barbosa, Cláudia Oliveira, Maria Alice Melo-Santos, Lindomar Pena, Marli Cordeiro, Rafael Franca, André Oliveira, Christina Peixoto, Walter S Leal
Emerging Microbes and Infections, 2017
Identification of a major Quantitative Trait Locus determining resistance to the organophosphate temephos in the dengue vector mosquito Aedes aegypti
Marcelo H.S. Paiva, Diane D. Lovin, Akio Mori, Maria A.V. Melo-Santos, David W. Severson, Constância F.J. Ayres
Genomics, 2016
Susceptibility profile of Aedes aegypti from Santiago Island, Cabo Verde, to insecticides
Hélio Daniel Ribeiro Rocha, Marcelo Henrique Santos Paiva, Norma Machado Silva, Ana Paula de Araújo, Denise dos Reis da Rosa de Azevedo Camacho, Aires Januário Fernandes da Moura, Lara Ferrero Gómez, Constância Flávia Junqueira Ayres, Maria Alice Varjal de Melo Santos
Acta Tropica, 2015
Insecticide resistance in Aedes aegypti populations from Cearáe, Brazil
Estelita Pereira Lima, Marcelo Henrique Santos Paiva, Ana Paula de Araújo, Éllyda Vanessa Gomes da Silva, Ulisses Mariano da Silva, Lúcia Nogueira de Oliveira, Antonio Euzébio G Santana, Clarisse Nogueira Barbosa, Clovis C de Paiva Neto, Marilia OF Goulart, Craig Stephen Wilding, Constância Flávia Junqueira Ayres, Maria Alice V de Melo Santos
Parasites and Vectors, 2011
Resistance to the organophosphate temephos: Mechanisms, evolution and reversion in an Aedes aegypti laboratory strain from Brazil
M.A.V. Melo-Santos, J.J.M. Varjal-Melo, A.P. Araújo, T.C.S. Gomes, M.H.S. Paiva, L.N. Regis, A.F. Furtado, T. Magalhaes, M.L.G. Macoris, M.T.M. Andrighetti, C.F.J. Ayres
Acta Tropica, 2010
Ultrastructural analysis of microfilariae of Wuchereria bancrofti obtained from persistent carriers after repeated courses of diethylcarbamazine
L.C. Alves, M.G.S. Cavalcanti, H.R.C. Araújo, G.M. Silva, D.L. Veras, M.H.S. Paiva, F.A. Brayner
Micron, 2009
Ultrastructural and cytochemical aspects of Schistosoma mansoni cercaria
M.G.S. Cavalcanti, H.R.C. Araújo, M.H.S. Paiva, G.M. Silva, C.C.G.S. Barbosa, L.F. Silva, F.A. Brayner, L.C. Alves
Micron, 2009

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