Dr. Bijendra Kumar Singh

Scopus Publications

Chitosan-Based Nanoencapsulation of Ocimum americanum Essential Oil as Safe Green Preservative Against Fungi Infesting Stored Millets, Aflatoxin B1 Contamination, and Lipid Peroxidation
Bijendra Kumar Singh, Shikha Tiwari, Akash Maurya, Somenath Das, Vipin Kumar Singh, Nawal Kishore Dubey
Food and Bioprocess Technology, 2023
Ethnobotanical knowledge as booster to Make in India campaign for agriculture and pharma sectors
Indian Journal of Traditional Knowledge, 2023
Aflatoxins in food systems: recent advances in toxicology, biosynthesis, regulation and mitigation through green nanoformulations
Shikha Tiwari, Bijendra Kumar Singh, Nawal Kishore Dubey
Journal of the Science of Food and Agriculture, 2023
Aflatoxins are hepatocarcinogenic and immunosuppressive mycotoxins mainly synthesized by Aspergillus flavus, A. parasiticus and A. nomius in food systems, causing negative health impacts to humans and other organisms. Aflatoxins contaminate most of the agri‐products of tropical and subtropical regions due to hot and humid conditions and persist in food items even after food processing steps, causing major threat towards the food security. Different physical and chemical strategies have been applied to mitigate aflatoxin contamination. However, negative impacts of chemical preservatives towards health and environment limit their practical applicability. In this regard, plant‐based preservatives, due to their economical, eco‐friendly and safer profile, are considered as a sustainable approach towards food safety. Incorporation of nanotechnology would enhance the bio‐efficacy of green preservatives by overcoming some of their major challenges, such as volatility. The present review deals with recent information on toxicology and molecular and enzymatic regulatory pathways in the biosynthesis of aflatoxins in food systems. A proper understanding of the role of different genes and regulatory proteins may provide novel preventive strategies for aflatoxin detoxification and also in development of aflatoxin‐resistant food items. The review also emphasizes the role of green nanoformulations as a sustainable approach towards the management of aflatoxins in food systems. In addition, some technological challenges of green nanotechnology have also been discussed in this review, along with highlighting some future perspectives. © 2022 Society of Chemical Industry.
Green facile synthesis of cajuput (Melaleuca cajuputi Powell.) essential oil loaded chitosan film and evaluation of its effectiveness on shelf-life extension of white button mushroom
Anand Kumar Chaudhari, Somenath Das, Bijendra Kumar Singh, Nawal Kishore Dubey
Food Chemistry, 2023
High speed homogenization assisted encapsulation of synergistic essential oils formulation: Characterization, in vitro release study, safety profile, and efficacy towards mitigation of aflatoxin B1 induced deterioration in rice samples
Somenath Das, Anand Kumar Chaudhari, Vipin Kumar Singh, Bijendra Kumar Singh, Nawal Kishore Dubey
Food and Chemical Toxicology, 2022
Preparation and characterization of a novel nanoemulsion consisting of chitosan and Cinnamomum tamala essential oil and its effect on shelf-life lengthening of stored millets
Bijendra Kumar Singh, Anand Kumar Chaudhari, Somenath Das, Shikha Tiwari, Nawal Kishore Dubey
Pesticide Biochemistry and Physiology, 2022
Chitosan encompassed Aniba rosaeodora essential oil as innovative green candidate for antifungal and antiaflatoxigenic activity in millets with emphasis on cellular and its mode of action
Bijendra Kumar Singh, Anand Kumar Chaudhari, Somenath Das, Shikha Tiwari, Akash Maurya, Vipin Kumar Singh, Nawal Kishore Dubey
Frontiers in Microbiology, 2022
The present study demonstrates first time investigation on encapsulation of Aniba rosaeodora essential oil into chitosan nanoemulsion (AREO-CsNe) with the aim of improvement of its antifungal, and aflatoxin B1 (AFB1) inhibitory performance in real food system. The GC–MS analysis of AREO revealed the presence of linalool (81.46%) as a major component. The successful encapsulation of EO into CsNe was confirmed through SEM, FTIR, and XRD analysis. The in-vitro release study showed the controlled release of AREO. AREO-CsNe caused complete inhibition of Aspergillus flavus (AFLHPSi-1) growth and AFB1 production at 0.8 and 0.6 μl/ml, respectively, which was far better than AREO (1.4 and 1.2 μl/ml, respectively). Impairment of ergosterol biosynthesis coupled with enhancement of cellular materials leakage confirmed plasma membrane as the possible antifungal target of both AREO and AREO-CsNe. Significant inhibition of methylglyoxal (AFB1 inducer) synthesis in AFLHPSi-1 cells by AREO and AREO-CsNe confirmed their novel antiaflatoxigenic mode of action. In-silico molecular docking studies revealed effective interaction of linalool with Ver-1 and Omt-A proteins, leading to inhibition of AFB1 biosynthesis. Further, AREO-CsNe showed enhanced antioxidant activity with IC50 values 3.792 and 1.706 μl/ml against DPPH• and ABTS•+ radicals, respectively. In addition, AREO-CsNe caused 100% protection of stored millets (Setaria italica seeds) from AFB1 contamination and lipid peroxidation over a period of 1 year without compromising its sensory properties and exhibited high safety profile with LD50 value 9538.742 μl/kg body weight. Based on enhanced performance of AREO-CsNe over AREO, it can be recommended as a novel substitute of synthetic preservative for preservation of stored millets.
Nanoencapsulation strategies for improving nutritional functionality, safety and delivery of plant-based foods: Recent updates and future opportunities
Monisha Soni, Akash Maurya, Somenath Das, Jitendra Prasad, Arati Yadav, Vipin Kumar Singh, Bijendra Kumar Singh, Nawal Kishore Dubey, Abhishek Kumar Dwivedy
Plant Nano Biology, 2022
abstract: Currently, food industries largely based on animal products including egg, meat, and fish are facing a burning challenge to meet the continuously increasing consumer demand. The greater availability of plant-based foods has aided the transition from animal foods to plant foods due to its sustainable, convenient, and affordable nature. Different plant derived ingredients like globular proteins, oligosaccharides, dietary fibres, starch, amylopectin, short and long chain unsaturated fatty acids are used to develop plant based foods, however, selection of most important ones being critical for creating a successful end product. Most notably, when the use of bioactive ingredients is targeted, some challenges like instability and reaction with other food matrices persist in development of plant based foods. In this context, nanoencapsulation of food components is an emerging and innovative field for controlled and targeted delivery with various prophylactic activities. The technology encompassing nanoencapsulation facilitates the release of food components, improvement in bioaccessibility, and digestion in the human body. Moreover, the plant-based foods are gaining cumulative attention in the scientific community due to development of various analogues of meat, egg, and milk. This review describes the recent updates in scientific understanding of plant based foods highlighting their prophylactic measures, market demand, and plant based ingredients with physical, functional, and molecular properties, and encapsulation strategies for elimination of various hurdles, maintenance of food quality, and insurances of safety as well as bioavailability of beneficial nutrients.
Nanoencapsulated Lippia origanoides essential oil: physiochemical characterisation and assessment of its bio-efficacy against fungal and aflatoxin contamination as novel green preservative
Shikha Tiwari, Neha Upadhyay, Bijendra Kumar Singh, Nawal K. Dubey, Abhishek K. Dwivedy, Vipin Kumar Singh
International Journal of Food Science and Technology, 2022
SummaryThe study explores in vitro antifungal and aflatoxin B1 inhibitory potency of chemically characterised Lippia origanoides EO (LOEO) encompassed in chitosan nanoparticle (CS‐LOEO‐Np). CS‐LOEO‐Np was physico‐chemically characterised through XRD, SEM and FTIR analyses. CS‐LOEO‐Np exhibited improved antifungal and AFB1 inhibitory efficacy (0.05 and 0.045 µl mL−1, respectively) in contrast to LOEO (0.30 and 0.25 µl mL−1, respectively). Bioactivity of LOEO loaded nanoparticle was enhanced as reflected by depletion in ergosterol content, rapid cellular ion release and reduced methylglyoxal content. IC50 value equivalent to 4.17 µl mL−1 displayed better antioxidant potency of CS‐LOEO‐Np as compared to LOEO (IC50 = 6.20 µl mL−1). CS‐LOEO‐Np preserved sensorial attributes of stored Nigella sativa (model food matrix) samples and have higher lethal toxicity dose, that is LD50 = 8832 mg kg−1. Hence, CS‐LOEO‐Np could serve as novel green candidate to ensure food security with better nutritional and sensorial features.
Facile Fabrication of Nanoformulated Cinnamomum glaucescens Essential Oil as a Novel Green Strategy to Boost Potency Against Food Borne Fungi, Aflatoxin Synthesis, and Lipid Oxidation
Shikha Tiwari, Neha Upadhyay, Bijendra Kumar Singh, Vipin Kumar Singh, Nawal Kishore Dubey
Food and Bioprocess Technology, 2022
Phytoextraction of heavy metals: Challenges and opportunities
Jitendra Prasad, Shikha Tiwari, Bijendra Kumar Singh, Nawal Kishore Dubey
Phytoremediation Technology for the Removal of Heavy Metals and Other Contaminants from Soil and Water, 2022
Boosting modern technologies with emphasis on biological approaches to potentiate prevention and control of aflatoxins: recent advances
Shikha Tiwari, Bijendra Kumar Singh, Vatsala Kishore, Nawal Kishore Dubey
Toxin Reviews, 2022
Fungal and mycotoxin contamination of herbal raw materials and their protection by nanoencapsulated essential oils: An overview
Bijendra Kumar Singh, Shikha Tiwari, Akash Maurya, Susheel Kumar, Nawal Kishore Dubey
Biocatalysis and Agricultural Biotechnology, 2022
Chemically characterized nanoencapsulated Homalomena aromatica Schott. essential oil as green preservative against fungal and aflatoxin B1 contamination of stored spices based on in vitro and in situ efficacy and favorable safety profile on mice
Shikha Tiwari, Neha Upadhyay, Bijendra Kumar Singh, Vipin Kumar Singh, Nawal Kishore Dubey
Environmental Science and Pollution Research, 2022
Mechanistic investigations on antifungal and antiaflatoxigenic activities of chemically characterised Carum carvi L. essential oil against fungal infestation and aflatoxin contamination of herbal raw materials
Akash Maurya, Susheel Kumar, Bijendra Kumar Singh, Anand Kumar Chaudhari, Abhishek Kumar Dwivedy, Bhanu Prakash, Nawal Kishore Dubey
Natural Product Research, 2022
Essential oils and their nanoformulations as green preservatives to boost food safety against mycotoxin contamination of food commodities: a review
Bijendra Kumar Singh, Shikha Tiwari, Nawal Kishore Dubey
Journal of the Science of Food and Agriculture, 2021
Secondary Metabolites of Higher Plants as Green Preservatives of Herbal Raw Materials and Their Active Principles During Postharvest Processing
Somenath Das, Vipin Kumar Singh, Neha Upadhyay, Bijendra Kumar Singh, Jitendra Prasad, Shikha Tiwari, Nawal Kishore Dubey
Evidence Based Validation of Traditional Medicines A Comprehensive Approach, 2021
Antimicrobial, Aflatoxin B1 Inhibitory and Lipid Oxidation Suppressing Potential of Anethole-Based Chitosan Nanoemulsion as Novel Preservative for Protection of Stored Maize
Anand Kumar Chaudhari, Vipin Kumar Singh, Somenath Das, Deepika, Bijendra Kumar Singh, Nawal Kishore Dubey
Food and Bioprocess Technology, 2020
Herbal Medicines as a Rational Alternative for Treatment of Human Diseases
Anand Kumar Chaudhari, Somenath Das, Bijendra Kumar Singh, Jitendra Prasad, Nawal Kishore Dubey, Abhishek Kumar Dwivedy
Botanical Leads for Drug Discovery, 2020

RECENT SCHOLAR PUBLICATIONS

Epigenetic Regulation in Plant–Microbe Interaction and Plant Immunity
BK Singh, A Chandran, S Singh
Epigenetic Regulations in Plants, 292-303 , 2026
2026
Citations: 1
Chitosan nanoemulsion incorporated with Carum carvi essential oil as ecofriendly alternative for mitigation of aflatoxin B1 contamination in stored herbal raw materials
S Das, A Maurya, VK Singh, AK Chaudhari, BK Singh, AK Dwivedy, ...
International Journal of Biological Macromolecules 270, 132248 , 2024
2024
Citations: 10
Antioxidant activity of essential oils: A mechanistic approach
BK Singh, A Maurya
Plant Essential Oils: From Traditional to Modern-Day Application, 59-76 , 2023
2023
Citations: 10
Chitosan-based nanoencapsulation of Ocimum americanum essential oil as safe green preservative against fungi infesting stored millets, aflatoxin B1 contamination, and lipid …
BK Singh, S Tiwari, A Maurya, S Das, VK Singh, NK Dubey
Food and Bioprocess Technology 16 (8), 1851-1872 , 2023
2023
Citations: 26
Green facile synthesis of cajuput (Melaleuca cajuputi Powell.) essential oil loaded chitosan film and evaluation of its effectiveness on shelf-life extension of white button …
AK Chaudhari, S Das, BK Singh, NK Dubey
Food Chemistry 401, 134114 , 2023
2023
Citations: 54
In vitro and in situ khasans sobti, Cyperus combination against tw
M Kumar, BK Singh, K Dwivedy, K Dwivedy, R Tilak, NK Dubey
Journal of Scientific Research 67 (2) , 2023
2023
High speed homogenization assisted encapsulation of synergistic essential oils formulation: Characterization, in vitro release study, safety profile, and efficacy towards …
S Das, AK Chaudhari, VK Singh, BK Singh, NK Dubey
Food and Chemical Toxicology 169, 113443 , 2022
2022
Citations: 18
Preparation and characterization of a novel nanoemulsion consisting of chitosan and Cinnamomum tamala essential oil and its effect on shelf-life lengthening of stored millets
BK Singh, AK Chaudhari, S Das, S Tiwari, NK Dubey
Pesticide Biochemistry and Physiology 187, 105214 , 2022
2022
Citations: 17
Mechanistic investigations on antifungal and antiaflatoxigenic activities of chemically characterised Carum carvi L. essential oil against fungal infestation and …
A Maurya, S Kumar, BK Singh, AK Chaudhari, AK Dwivedy, B Prakash, ...
Natural Product Research 36 (17), 4563-4568 , 2022
2022
Citations: 16
Bioactive components and biological properties of Zingiber officinale roscoe essential oil
BK Singh, NK Dubey
Bioactives and Pharmacology of Medicinal Plants, 391-401 , 2022
2022
Citations: 2
Chitosan encompassed Aniba rosaeodora essential oil as innovative green candidate for antifungal and antiaflatoxigenic activity in millets with emphasis on …
BK Singh, AK Chaudhari, S Das, S Tiwari, A Maurya, VK Singh, NK Dubey
Frontiers in Microbiology 13, 970670 , 2022
2022
Citations: 25
Boosting modern technologies with emphasis on biological approaches to potentiate prevention and control of aflatoxins: recent advances
S Tiwari, BK Singh, V Kishore, NK Dubey
Toxin Reviews 41 (3), 1007-1017 , 2022
2022
Citations: 8
Nanoencapsulation strategies for improving nutritional functionality, safety and delivery of plant-based foods: Recent updates and future opportunities
M Soni, A Maurya, S Das, J Prasad, A Yadav, VK Singh, BK Singh, ...
Plant Nano Biology 1, 100004 , 2022
2022
Citations: 44
Nanoencapsulated Lippia origanoides essential oil: physiochemical characterisation and assessment of its bio-efficacy against fungal and aflatoxin contamination …
S Tiwari, N Upadhyay, BK Singh, NK Dubey, AK Dwivedy, VK Singh
International Journal of Food Science and Technology 57 (4), 2216-2225 , 2022
2022
Citations: 9
Facile Fabrication of Nanoformulated Cinnamomum glaucescens Essential Oil as a Novel Green Strategy to Boost Potency Against Food Borne Fungi, Aflatoxin …
S Tiwari, N Upadhyay, BK Singh, VK Singh, NK Dubey
Food and Bioprocess Technology 15 (2), 319-337 , 2022
2022
Citations: 26
Phytoextraction of heavy metals: Challenges and opportunities
J Prasad, S Tiwari, BK Singh, NK Dubey
Phytoremediation technology for the removal of heavy metals and other … , 2022
2022
Citations: 16
Fungal and mycotoxin contamination of herbal raw materials and their protection by nanoencapsulated essential oils: An overview
BK Singh, S Tiwari, A Maurya, S Kumar, NK Dubey
Biocatalysis and Agricultural Biotechnology 39, 102257 , 2022
2022
Citations: 13
Chemically characterized nanoencapsulated Homalomena aromatica Schott. essential oil as green preservative against fungal and aflatoxin B1 contamination of stored spices based …
S Tiwari, N Upadhyay, BK Singh, VK Singh, NK Dubey
Environmental Science and Pollution Research 29 (2), 3091-3106 , 2022
2022
Citations: 24
Essential oils and their nanoformulations as green preservatives to boost food safety against mycotoxin contamination of food commodities: a review
BK Singh, S Tiwari, NK Dubey
Journal of the Science of Food and Agriculture 101 (12), 4879-4890 , 2021
2021
Citations: 100
Secondary metabolites of higher plants as green preservatives of herbal raw materials and their active principles during postharvest processing
S Das, VK Singh, N Upadhyay, BK Singh, J Prasad, S Tiwari, NK Dubey
Evidence Based Validation of Traditional Medicines: A comprehensive Approach … , 2021
2021
Citations: 5

MOST CITED SCHOLAR PUBLICATIONS

Essential oils and their nanoformulations as green preservatives to boost food safety against mycotoxin contamination of food commodities: a review
BK Singh, S Tiwari, NK Dubey
Journal of the Science of Food and Agriculture 101 (12), 4879-4890 , 2021
2021
Citations: 100
Antimicrobial, Aflatoxin B 1 Inhibitory and Lipid Oxidation Suppressing Potential of Anethole-Based Chitosan Nanoemulsion as Novel Preservative for Protection of …
AK Chaudhari, VK Singh, S Das, Deepika, BK Singh, NK Dubey
Food and Bioprocess Technology 13 (8), 1462-1477 , 2020
2020
Citations: 61
Green facile synthesis of cajuput (Melaleuca cajuputi Powell.) essential oil loaded chitosan film and evaluation of its effectiveness on shelf-life extension of white button …
AK Chaudhari, S Das, BK Singh, NK Dubey
Food Chemistry 401, 134114 , 2023
2023
Citations: 54
Nanoencapsulation strategies for improving nutritional functionality, safety and delivery of plant-based foods: Recent updates and future opportunities
M Soni, A Maurya, S Das, J Prasad, A Yadav, VK Singh, BK Singh, ...
Plant Nano Biology 1, 100004 , 2022
2022
Citations: 44
Encapsulation of essential oils-a booster to enhance their bio-efficacy as botanical preservatives
S Tiwari, BK Singh, NK Dubey
J. Sci. Res 64 (1), 175-178 , 2020
2020
Citations: 29
Chitosan-based nanoencapsulation of Ocimum americanum essential oil as safe green preservative against fungi infesting stored millets, aflatoxin B1 contamination, and lipid …
BK Singh, S Tiwari, A Maurya, S Das, VK Singh, NK Dubey
Food and Bioprocess Technology 16 (8), 1851-1872 , 2023
2023
Citations: 26
Facile Fabrication of Nanoformulated Cinnamomum glaucescens Essential Oil as a Novel Green Strategy to Boost Potency Against Food Borne Fungi, Aflatoxin …
S Tiwari, N Upadhyay, BK Singh, VK Singh, NK Dubey
Food and Bioprocess Technology 15 (2), 319-337 , 2022
2022
Citations: 26
Chitosan encompassed Aniba rosaeodora essential oil as innovative green candidate for antifungal and antiaflatoxigenic activity in millets with emphasis on …
BK Singh, AK Chaudhari, S Das, S Tiwari, A Maurya, VK Singh, NK Dubey
Frontiers in Microbiology 13, 970670 , 2022
2022
Citations: 25
Chemically characterized nanoencapsulated Homalomena aromatica Schott. essential oil as green preservative against fungal and aflatoxin B1 contamination of stored spices based …
S Tiwari, N Upadhyay, BK Singh, VK Singh, NK Dubey
Environmental Science and Pollution Research 29 (2), 3091-3106 , 2022
2022
Citations: 24
High speed homogenization assisted encapsulation of synergistic essential oils formulation: Characterization, in vitro release study, safety profile, and efficacy towards …
S Das, AK Chaudhari, VK Singh, BK Singh, NK Dubey
Food and Chemical Toxicology 169, 113443 , 2022
2022
Citations: 18
Herbal medicines as a rational alternative for treatment of human diseases
AK Chaudhari, S Das, BK Singh, J Prasad, NK Dubey, AK Dwivedy
Botanical leads for drug discovery, 29-49 , 2020
2020
Citations: 18
Preparation and characterization of a novel nanoemulsion consisting of chitosan and Cinnamomum tamala essential oil and its effect on shelf-life lengthening of stored millets
BK Singh, AK Chaudhari, S Das, S Tiwari, NK Dubey
Pesticide Biochemistry and Physiology 187, 105214 , 2022
2022
Citations: 17
Mechanistic investigations on antifungal and antiaflatoxigenic activities of chemically characterised Carum carvi L. essential oil against fungal infestation and …
A Maurya, S Kumar, BK Singh, AK Chaudhari, AK Dwivedy, B Prakash, ...
Natural Product Research 36 (17), 4563-4568 , 2022
2022
Citations: 16
Phytoextraction of heavy metals: Challenges and opportunities
J Prasad, S Tiwari, BK Singh, NK Dubey
Phytoremediation technology for the removal of heavy metals and other … , 2022
2022
Citations: 16
Fungal and mycotoxin contamination of herbal raw materials and their protection by nanoencapsulated essential oils: An overview
BK Singh, S Tiwari, A Maurya, S Kumar, NK Dubey
Biocatalysis and Agricultural Biotechnology 39, 102257 , 2022
2022
Citations: 13
Chitosan nanoemulsion incorporated with Carum carvi essential oil as ecofriendly alternative for mitigation of aflatoxin B1 contamination in stored herbal raw materials
S Das, A Maurya, VK Singh, AK Chaudhari, BK Singh, AK Dwivedy, ...
International Journal of Biological Macromolecules 270, 132248 , 2024
2024
Citations: 10
Antioxidant activity of essential oils: A mechanistic approach
BK Singh, A Maurya
Plant Essential Oils: From Traditional to Modern-Day Application, 59-76 , 2023
2023
Citations: 10
Nanoencapsulated Lippia origanoides essential oil: physiochemical characterisation and assessment of its bio-efficacy against fungal and aflatoxin contamination …
S Tiwari, N Upadhyay, BK Singh, NK Dubey, AK Dwivedy, VK Singh
International Journal of Food Science and Technology 57 (4), 2216-2225 , 2022
2022
Citations: 9
Boosting modern technologies with emphasis on biological approaches to potentiate prevention and control of aflatoxins: recent advances
S Tiwari, BK Singh, V Kishore, NK Dubey
Toxin Reviews 41 (3), 1007-1017 , 2022
2022
Citations: 8
Secondary metabolites of higher plants as green preservatives of herbal raw materials and their active principles during postharvest processing
S Das, VK Singh, N Upadhyay, BK Singh, J Prasad, S Tiwari, NK Dubey
Evidence Based Validation of Traditional Medicines: A comprehensive Approach … , 2021
2021
Citations: 5

Dr. Bijendra Kumar Singh

EDUCATION

RESEARCH, TEACHING, or OTHER INTERESTS

Scopus Publications

RECENT SCHOLAR PUBLICATIONS

MOST CITED SCHOLAR PUBLICATIONS