Animal sporotrichosis in two municipalities in Mato Grosso do Sul - Brazil: epidemiological aspects from a One Health perspective Laura Tathianne Ramos Araújo, Walkíria Arruda da Silva, Aiesca Oliveira Pellegrin, Luciana Escalante Pereira, Cassia Rejane Brito Leal, Urbano Gomes Pinto de Abreu, Raquel Soares Juliano Ciencia Animal Brasileira, 2024 Human and animal sporotrichosis has been observed in Corumbá and Ladário, Mato Grosso do Sul (MS), Brazil, since 2011. The unique characteristics of border areas and the human-environmentanimal interface necessitate the development of health policies and programs that incorporate One Health concepts, fostering advances in health research and management. This article examines the epidemiological aspects of feline sporotrichosis with the aim of supporting decisions pertinent to its prevention, control, and public health management. Data were collected from veterinary records between 2011 and 2018, encompassing 315 cases (five dogs and 310 cats). Mapping of compatible or confirmed cases of animal sporotrichosis was conducted using QGis, though data loss posed a significant limitation. Descriptive statistics assessed the frequency of variables, geo-statistical analysis methods, and disease occurrence rates. This study represents the first investigation of sporotrichosis in the state. The occurrence rate in felines varied from 0.04% to 3.50% between 2015 and 2018. The typical profile of infected cats was young adults that were not neutered. The euthanasia rate was 76.50%, while the cure rate for treated animals stood at 64.90%. A higher number of cases was associated with more populous neighborhoods, indicating these as higher-risk areas. The findings were crucial in engaging public authorities and the community to address sporotrichosis. However, improvements are needed in areas such as case notification, laboratory diagnosis, treatment monitoring, health education, responsible pet ownership, and population control of felines.
Pathological aspects of a septicemic salmonellosis outbreak caused by Salmonella serotype Typhimurium in captive blue-fronted Amazon parrots (Amazona aestiva) Jecelen A. Campos, Carolina C. Guizelini, Saulo P. Pavarini, Lucas B.S. Azuaga, Michelli L. Souza, Cássia R.B. Leal, Carlos A.N. Ramos, Danilo C. Gomes Pesquisa Veterinaria Brasileira, 2024 There are reports of septicemic salmonellosis in exotic birds caused by Salmonella enterica serotype Typhimurium, although they are scarce and do not address the anatomopathological and immunohistochemical aspects of this condition in blue-fronted Amazon parrots (Amazona aestiva). This study aims to report an outbreak of salmonellosis by S. Typhimurium in 15 blue-fronted parrots introduced to a rehabilitation center in the state of Mato Grosso do Sul, Brazil. Hepatomegaly and splenomegaly, accompanied by flat white areas in the capsule and parenchyma, were the most frequent gross changes, histologically represented by necrotic and heterophilic hepatitis and splenitis and associated with bacteria in the cytoplasm of macrophages and free in the affected tissue. S. Typhimurium was identified from bacterial culture, PCR, and DNA sequencing. Along with immunohistochemistry samples of liver, spleen, kidney, small intestine, pancreas, thyroid, heart, and lung, immunostaining was used for Salmonella spp. without previous bacterial isolation. The lethality of this outbreak demonstrates the relevance of salmonellosis in blue-fronted parrots, which are known to be carriers of this bacteria.
Molecular and phenotypic characterization of Escherichia coli from calves in an important meat-producing region in Brazil Juliane F Tutija, Carlos AN Ramos, Ricardo AA Lemos, Amanda AL Santos, Guilherme H Reckziegel, Mariana G Freitas, Cassia RB Leal Journal of Infection in Developing Countries, 2022 Introduction: Escherichia coli strains that lead to enteritis are considered an important cause of diarrhea in calves. For correct identification, these microorganisms must be differentiated from non-pathogenic members of the intestinal microbiota. The aim of the present work was to characterize E. coli isolates in calves regarding the presence of virulence genes that cause enteritis and evaluate the sensitivity of the isolates to different antimicrobials.
 Methodology: One hundred forty-nine samples from beef cattle and 27 samples from dairy cattle were evaluated. All samples were submitted to microbiological identification and the disk diffusion antibiogram test. The polymerase chain reaction method was used to detect virulence genes.
 Results: A hundred seventy-six samples were biochemically identified as E. coli and antibiograms were determined. The samples were then submitted to PCR; 35 were positive for the eae gene (19.88%), 135 (76.70%) for the stx1 gene, 62 (35.22%) for the stx2 gene, 159 (90.34%) for the sta gene and 35 (19.88%) for the ltII gene. No samples were positive for the cnf gene. Based on these results, the E. coli isolates were classified into pathotypes: enteropathogenic (n = 3), enterohemorrhagic (n = 32), Shiga toxin-producing (n = 122) and enterotoxigenic (n = 163). The antimicrobial sensitivity tests revealed that 77.2% of the isolates were resistant to three or more pharmacological groups, characterizing these isolates as multidrug resistant.
 Conclusions: Enterotoxigenic E. coli was the predominant pathotype. Moreover, the prevalence of multidrug-resistant isolates was very high, accounting for the vast majority of isolates.
Granulomatous tracheitis and dermatitis due to Curvularia sp. in a horse: case report É.V. Fiorin, M.C.B. Brigatto, P.H.A. Jardim, M.R. Chang, C.R.B. Leal, M.A. Araújo Arquivo Brasileiro De Medicina Veterinaria E Zootecnia, 2021 Tracheal fungal infections in horses are rare. This case report describes surgical and clinical management of a filly with a Curvularia sp. infection within the trachea and skin that caused severe intraluminal granulomas and cutaneous nodules, respectively. The patient was successfully treated with itraconazole and surgical excision.
Antimicrobial potential of essential oils from cerrado plants against multidrug-resistant foodborne microorganisms Genilson Silva de Jesus, Ana Camila Micheletti, Rafael Gonçalves Padilha, Jessica de Souza de Paula, Flavio Macedo Alves, Cassia Rejane Brito Leal, Fernanda Rodrigues Garcez, Walmir Silva Garcez, Nidia Cristiane Yoshida Molecules, 2020 Foodborne pathogens are a real public health concern in an escalating antimicrobial resistance scenario. Natural products represent a promising source of bioactive molecules, and essential oils have attracted much attention due to their myriad of biological properties, including antibacterial activities. In this context, essential oils obtained from the leaves of Chromolaena squalida, Campomanesia sessiliflora, Myrsine guianensis, Matayba guianensis, Siparuna guianensis, Ocotea minarum and Endlicheria paniculata—species from the Cerrado biome of Midwest Brazil—were extracted and evaluated for their antibacterial activity against a panel of four standard and three clinical multidrug−resistant bacterial strains. All tested oils showed moderate to good activity against at least four bacterial strains, including Salmonella Typhi and oxacillin−resistant Staphylococcus. The essential oils from C. squalida, C. sessiliflora, My. guianensis and Ma. guianensis showed strong inhibition of clinical Staphylococcus strains, which cause bovine mastitis and are related to milk−borne diseases. Their chemical profiles were investigated by gas chromatography coupled to mass spectrometry (GC/MS), which revealed a predominance of mono− and sesquiterpene hydrocarbons, some of which with well−known antimicrobial properties. The essential oil from Cerrado plants proved active against resistant Gram−positive and Gram−negative bacteria, revealing their potentialities for the development of new alternative agents to prevent the spreading of resistant bacterial contamination.
Outbreak investigation of septicemic salmonellosis in calves Carolina C Guizelini, Juliane F Tutija, Dayane R Morais, Flávia B Bacha, Carlos A N Ramos, Cássia R B Leal, Maria E Zaquetti, Ricardo A A Lemos Journal of Infection in Developing Countries, 2020
Salmonellosis in calves without intestinal lesions Carolina C. Guizelini, Rayane C. Pupin, Cássia R.B. Leal, Carlos A.N. Ramos, Saulo P. Pavarini, Danilo C. Gomes, Tessie B. Martins, Ricardo A.A. Lemos Pesquisa Veterinaria Brasileira, 2019
Propolis extract in the diet of weaned piglets Liliane Maria Piano Gonçalves, Charles Kiefer, Camilla Mendonça Silva, Cássia Rejane Brito Leal, Stephan Alexander da Silva Alencar, Kelly Cristina Nunes Carvalho, Luciana Moura Rufino Ciencia Rural, 2018
Photoinactivation of mcr-1 positive Escherichia coli C S A Caires, C R B Leal, A C S Rodrigues, A R Lima, C M Silva, C A N Ramos, M R Chang, E J Arruda, S L Oliveira, V A Nascimento, A R L Caires Laser Physics Letters, 2018
Propolis extraction residue like bacterial inhibitor "in vitro" Natália da Silva HEIMBACH, Camila Celeste Brandão Ferreira ÍTAVO, Cássia Rejane Brito LEAL, Luís Carlos Vinhas ÍTAVO, Jonilson Araújo da SILVA, Pâmila Carolini Gonçalves SILVA, Letícia Costa de REZENDE, Maria de Fátima Falcão GOMES Revista Brasileira De Saude E Producao Animal, 2016
First genetic identification of Cryptosporidium parvum subtype IIaA14G2R1in beef cattle in Brazil Rafael Pereira Heckler, Dyego Gonçalves Lino Borges, Flávia Barbieri Bacha, Marcel Kenzo Vilalba Onizuka, Letícia e Silva Teruya, Juliana Paniago Lordelo Neves, Cássia Rejane Brito Leal, Ricardo Antônio Amaral de Lemos, Marcelo Vasconcelos Meireles, Fernando de Almeida Borges Preventive Veterinary Medicine, 2015
Emergent outbreak of visceral leishmaniasis in Mato Grosso do Sul State Ana Lúcia Lyrio de Oliveira, Anamaria Mello Miranda Paniago, Maria Elizabeth Cavalheiros Dorval, Elisa Teruya Oshiro, Cássia Rejane Leal, Marcos Sanches, Rivaldo Venâncio da Cunha, Márcio Neves Bóia Revista Da Sociedade Brasileira De Medicina Tropical, 2006
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Pathological aspects of a septicemic salmonellosis outbreak caused by Salmonella serotype Typhimurium in captive blue-fronted Amazon parrots ( Amazona aestiva ) JA Campos, CC Guizelini, SP Pavarini, LBS Azuaga, ML Souza, ... Pesquisa Veterinária Brasileira 44, e07428 , 2024 2024 Citations: 2
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Aspectos patológicos de um surto de salmonelose septicêmica causado por Salmonella sorotipo Typhimurium em papagaios-verdadeiros ( Amazona aestiva ) de … JA Campos, CC Guizelini, SP Pavarini, L Azuaga, ML Souza, CRB Leal, ... Pesquisa Veterinária Brasileira 44, e07428 , 2024 2024
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Effects of platelet-rich plasma gel on skin healing in surgical wound in horses R DeRossi, ACAO Coelho, GS Mello, FO Frazílio, CRB Leal, GG Facco, ... Acta cirúrgica brasileira 24, 276-281 , 2009 2009 Citations: 186
Evidence that lipopolisaccharide may contribute to the cytokine storm and cellular activation in patients with visceral leishmaniasis JR Santos-Oliveira, EG Regis, CRB Leal, RV Cunha, PT Bozza, ... PLoS neglected tropical diseases 5 (7), e1198 , 2011 2011 Citations: 90
Foco emergente de leishmaniose visceral em Mato Grosso do Sul ALL Oliveira, AMM Paniago, MEC Dorval, ET Oshiro, CR Leal, M Sanches, ... Revista da Sociedade Brasileira de Medicina Tropical 39 (5), 446-450 , 2006 2006 Citations: 81
Scanning electron microscopy and microbiological evaluation of equine burn wound repair after platelet-rich plasma gel treatment FB Maciel, R DeRossi, TJC Módolo, RC Pagliosa, CRJ Leal, ... Burns 38 (7), 1058-1065 , 2012 2012 Citations: 68
Comparison between enzyme-linked immunosorbent assay, indirect fluorescent antibody and rapid conglutination tests in detecting antibodies against Babesia bovis FR Araujo, CR Madruga, CRB Leal, MAM Schenk, RH Kessler, ... Veterinary parasitology 74 (2-4), 101-108 , 1998 1998 Citations: 52
Photoinactivation effect of eosin methylene blue and chlorophyllin sodium-copper against Staphylococcus aureus and Escherichia coli CSA Caires, CRB Leal, CAN Ramos, D Bogo, AR Lima, EJ Arruda, ... Lasers in medical science 32 (5), 1081-1088 , 2017 2017 Citations: 46
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Evaluation of an enzyme-linked immunosorbent assay to detect antibodies against Anaplasma marginale CR Madruga, APC Marques, CRB Leal, CME Carvalho, FR Araújo, ... Pesquisa Veterinária Brasileira 20 (3), 109-112 , 2000 2000 Citations: 36
Asymptomatic infection in family contacts of patients with human visceral leishmaniasis in Três Lagoas, Mato Grosso do Sul State, Brazil ALL Oliveira, AMM Paniago, MA Sanches, MEC Dorval, ET Oshiro, ... Cadernos de saude publica 24, 2827-2833 , 2008 2008 Citations: 31
Doenças de ovinos diagnosticadas no Laboratório de Anatomia Patológica Animal da Universidade Federal de Mato Grosso do Sul (1996-2010) TL Almeida, KB Brum, RAA Lemos, CRB Leal, FA Borges Pesquisa Veterinária Brasileira 33 (1), 21-29 , 2013 2013 Citations: 28
Emergent outbreak of visceral leishmaniasis in Mato Grosso do Sul State ALL de Oliveira, AMM Paniago, MEC Dorval, ET Oshiro, CR Leal, ... Revista da Sociedade Brasileira de Medicina Tropical 39 (5) , 2006 2006 Citations: 28
Freqüência de anticorpos anti-Anaplasma marginale em rebanhos leiteiros da Bahia FR Araújo, CR Madruga, CR Leal, PA Bastos, AP Marques Arq. bras. med. vet. zootec, 243-6 , 1998 1998 Citations: 25
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First genetic identification of Cryptosporidium parvum subtype IIaA14G2R1in beef cattle in Brazil RP Heckler, DGL Borges, FB Bacha, MKV Onizuka, JPL Neves, CRB Leal, ... Preventive veterinary medicine 121 (3-4), 391-394 , 2015 2015 Citations: 17
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Resíduo da extração de própolis como inibidor bacteriano in vitro NS HEIMBACH, CCBF ÍTAVO, CRB LEAL, LCV ÍTAVO, JA SILVA, ... Revista Brasileira de Saúde e Produção Animal 17, 65-72 , 2016 2016 Citations: 14
Estimativas de perdas econômicas causadas por reação granulomatosa local após uso de vacina oleosa contra febre aftosa em bovinos de Mato Grosso do Sul PV Leal, RC Pupin, AC Santos, TC Faccin, E Surdi, CRB Leal, ... Pesquisa Veterinária Brasileira 34 (8), 738-742 , 2014 2014 Citations: 14
