Evaluation of Genistein as a Mitochondrial Modulator and Its Effects on Sperm Quality Marilia Ferigolo, Jessica Nardi, Natália Freddo, Alessandra Ferramosca, Vincenzo Zara, et al. International Journal of Molecular Sciences, 2023 Phytoestrogens, such as isoflavones, are bioactive compounds found in plants with defense and protection functions. In the human body, they simulate the behavior of the hormone estradiol and can modulate the function of the male hypothalamic–pituitary–gonadal axis. This study aims to describe the effects of genistein on sperm quality of Wistar rats (male/adult) after a short oral administration protocol (50 mg/day, for 5 days), focusing on mitochondrial function. No signs of toxicity were observed in the animals during the period. The testicular mass of rats from the genistein-treated group was lower than that from the control group. Isoflavone increased the number of viable Leydig and Sertoli cells, spermatogonia, and primary spermatocytes in the treated group. The rounded spermatid count was similar to the control group, and a decrease in elongated spermatids was observed in the treated group. Genistein treatment increased plasma testosterone levels in the treated group. To the best of our knowledge, this is the first report of an in vivo short protocol demonstrating that genistein administration stimulates the overall oxygen consumption in rat seminal samples. Therefore, genistein induced a pro-spermatogenesis effect, enhanced plasma testosterone levels, and increased oxygen consumption, improving sperm mitochondrial efficiency. Similar protocols can be explored in animal and human infertility issues.
Ototoxicity of cypermethrin in Wistar rats Eduarda Oliveira Cunha, Aléxia dos Reis, Mateus Belmonte Macedo, Márcia Salgado Machado, Eliane Dallegrave Brazilian Journal of Otorhinolaryngology, 2020
RECENT SCHOLAR PUBLICATIONS
Neurodegeneration of the mastoid segment of the human facial nerve associated with senescence ILM Rocha, J Lavinsky, MB Macedo, AUS Petry, T Malysz Brazilian Journal of Otorhinolaryngology 92 (3), 101784 , 2026 2026.0
EFFECTS OF THE HERBICIDES GLYPHOSATE AND 2, 4-DICHLOROPHENOXYACETIC ACID (2, 4-D) ON HEMATOLOGICAL PARAMETERS IN WISTAR RATS GG Adami, MB Macedo, D de Athayde Saul, T Malysz, IM Moeller, ... IV Congresso Latino-americano de Toxicologia Clínico-laboratorial 5 , 2025 2025.0
Subchronic Residual Oil Fly Ash (ROFA) exposure induces oxidative stress in brain, lung, and cardiac tissues and promotes neuroinflammation, with partial attenuation by taurine GN da Silva, B Marmett, MB Macedo, GP Dorneles, DA Saul, PRT Romão, ... NeuroToxicology 111, 103347 , 2025 2025.0
Morphometric evaluation of the recurrent laryngeal nerve of wistar rats exposed to pesticides MB Macedo, EO Cunha, A Dos Reis, MS Machado, D de Campos, ... Journal of Voice 38 (2), 264-272 , 2024 2024.0 Citations: 2
Evaluation of genistein as a mitochondrial modulator and its effects on sperm quality M Ferigolo, J Nardi, N Freddo, A Ferramosca, V Zara, E Dallegrave, ... International Journal of Molecular Sciences 24 (18), 14260 , 2023 2023.0 Citations: 5
Ototoxicidade da cipermetrina em ratos Wistar EO Cunha, A Reis, MB Macedo, MS Machado, E Dallegrave Brazilian Journal of Otorhinolaryngology 86, 587-592 , 2020 2020.0
Ototoxicity of cypermethrin in Wistar rats EO Cunha, A Dos Reis, MB Macedo, MS Machado, E Dallegrave Brazilian Journal of Otorhinolaryngology 86 (5), 587-592 , 2020 2020.0 Citations: 19
Who Donates the Body to Science? The Profile of the Donor in Southern Brazil AO da Rocha, JL Maues, MB Macedo, GDGR Thomaz, CSM Okabayashi, ... The FASEB Journal 32, 632.2-632.2 , 2018 2018.0
Why do people donate their bodies to science? AO da Rocha, GDGR Thomaz, CSM Okabayashi, JMA Picanço, JL Maues, ... The FASEB Journal 32, 632.3-632.3 , 2018 2018.0 Citations: 2
Museu de Anatomia: instrumento de democratização do ensino e da inclusão social AO Rocha, MB Macedo, MPO Moraes Proex/Unila , 2017 2017.0 Citations: 2
Validade do teste de sussuro no rastreio auditivo em longevos do Projeto Acompanhamento Multiprofissional ao Longevo de Porto Alegre (AMPAL) MA Bauer, CL Grigol, MB Macedo, ÂJG Bós, AR Teixeira Pajar: pan american journal of aging research. Porto Alegre, PUCRS , 2017 2017.0 Citations: 3
VIVA O POVO BRASILEIRO: PROJETO CÊNICO-MUSICAL DO CORAL UFCSPA MR dos Santos, AC Brasil, MB Macedo, YLM Rodrigues, LC Pellanda
Neurotoxic Effects of Subchronic Inhalatory Exposure to Organophosphate and Pyrethroid Pesticides in Wistar Rats C Schneider Ribeiro, MI Zaniratti da Rosa, G dos Santos Feijó, ... Available at SSRN 4294461 , 0
MOST CITED SCHOLAR PUBLICATIONS
Ototoxicity of cypermethrin in Wistar rats EO Cunha, A Dos Reis, MB Macedo, MS Machado, E Dallegrave Brazilian Journal of Otorhinolaryngology 86 (5), 587-592 , 2020 2020.0 Citations: 19
Evaluation of genistein as a mitochondrial modulator and its effects on sperm quality M Ferigolo, J Nardi, N Freddo, A Ferramosca, V Zara, E Dallegrave, ... International Journal of Molecular Sciences 24 (18), 14260 , 2023 2023.0 Citations: 5
Validade do teste de sussuro no rastreio auditivo em longevos do Projeto Acompanhamento Multiprofissional ao Longevo de Porto Alegre (AMPAL) MA Bauer, CL Grigol, MB Macedo, ÂJG Bós, AR Teixeira Pajar: pan american journal of aging research. Porto Alegre, PUCRS , 2017 2017.0 Citations: 3
Morphometric evaluation of the recurrent laryngeal nerve of wistar rats exposed to pesticides MB Macedo, EO Cunha, A Dos Reis, MS Machado, D de Campos, ... Journal of Voice 38 (2), 264-272 , 2024 2024.0 Citations: 2
Why do people donate their bodies to science? AO da Rocha, GDGR Thomaz, CSM Okabayashi, JMA Picanço, JL Maues, ... The FASEB Journal 32, 632.3-632.3 , 2018 2018.0 Citations: 2
Museu de Anatomia: instrumento de democratização do ensino e da inclusão social AO Rocha, MB Macedo, MPO Moraes Proex/Unila , 2017 2017.0 Citations: 2
Neurodegeneration of the mastoid segment of the human facial nerve associated with senescence ILM Rocha, J Lavinsky, MB Macedo, AUS Petry, T Malysz Brazilian Journal of Otorhinolaryngology 92 (3), 101784 , 2026 2026.0
EFFECTS OF THE HERBICIDES GLYPHOSATE AND 2, 4-DICHLOROPHENOXYACETIC ACID (2, 4-D) ON HEMATOLOGICAL PARAMETERS IN WISTAR RATS GG Adami, MB Macedo, D de Athayde Saul, T Malysz, IM Moeller, ... IV Congresso Latino-americano de Toxicologia Clínico-laboratorial 5 , 2025 2025.0
Subchronic Residual Oil Fly Ash (ROFA) exposure induces oxidative stress in brain, lung, and cardiac tissues and promotes neuroinflammation, with partial attenuation by taurine GN da Silva, B Marmett, MB Macedo, GP Dorneles, DA Saul, PRT Romão, ... NeuroToxicology 111, 103347 , 2025 2025.0
Ototoxicidade da cipermetrina em ratos Wistar EO Cunha, A Reis, MB Macedo, MS Machado, E Dallegrave Brazilian Journal of Otorhinolaryngology 86, 587-592 , 2020 2020.0
Who Donates the Body to Science? The Profile of the Donor in Southern Brazil AO da Rocha, JL Maues, MB Macedo, GDGR Thomaz, CSM Okabayashi, ... The FASEB Journal 32, 632.2-632.2 , 2018 2018.0
VIVA O POVO BRASILEIRO: PROJETO CÊNICO-MUSICAL DO CORAL UFCSPA MR dos Santos, AC Brasil, MB Macedo, YLM Rodrigues, LC Pellanda
Neurotoxic Effects of Subchronic Inhalatory Exposure to Organophosphate and Pyrethroid Pesticides in Wistar Rats C Schneider Ribeiro, MI Zaniratti da Rosa, G dos Santos Feijó, ... Available at SSRN 4294461 , 0
