Accumulation of heavy metals in the body and nest of ants in traditional oil mining geosite Wonocolo, Bojonegoro Geopark, East Java, Indonesia Laily Agustina Rahmawati, Dwi Oktafitria, Nova Nevila Rodhi, Dyah Setyaningrum, Ahmad Ni'amul Abrori, Intan Ayu Pebiola, Tria Agustining Tyas, Santi Amanda Tiara Putri, Evi Cici Kumala, Abilsa Duanda Ramadhan Firdaus Journal of Ecological Engineering, 2025 Traditional oil mining in Wonocolo, Kedewan District, Bojonegoro Regency, East Java Province, Indonesia, causes soil contamination, also by heavy metals.On the basis of bioindicators, 63.3% of soil macrofauna are dominated by ants (Hymenoptera), which have the potential to be heavy metal bioaccumulators.This study aimed to analyze the types and amounts of heavy metal accumulation in the bodies and nests of ants found in Wonocolo.Samples were collected using exploration and hand shorting methods, while heavy metal accumulation was analyzed using XRF.The results showed that 13 types of ants collected accumulated heavy metals in their bodies and nests.Ten types of heavy metals were identified in the bodies of ants, with 3 of them found in all types of ants, namely Cu, Fe, and Zn.Thirteen types of heavy metals were also identified in the nests, with 6 of them found in all nests, namely Al, Fe, Mn, Sr, Ti, and Zn.On the basis of the number of metal type of accumulated, four types of ants have the potential to be bioaccumulators, namely: Camponotus pennsylvanicus, Tetramorium bicarinatum, Solenopsis invicta, and Cerebara colobobsis.The detection of various types of heavy metals in the bodies and nests of ants in the Wonocolo traditional oil mining area suggest that the ants in this area can potentially act as heavy metal bioaccumulators that can help the remediation process of soil contaminated by traditional oil mining activities.
Plastid DNA Barcoding and RtActin cDNA Fragment Isolation of Reutealis Trisperma: A Promising Bioresource for Biodiesel Production Nurul Jadid, Nur Laili Alfina Rosidah, Muhammad Rifqi Nur Ramadani, Indah Prasetyowati, Noor Nailis Sa’adah, Aulia Febrianti Widodo, Dwi Oktafitria Bioinformatics and Biology Insights, 2023 Reutealis trisperma belonging to the family Euphorbiaceae is currently used for biodiesel production, and rapid development in plant-based biofuel production has led to its increasing demand. However, massive utilization of bio-industrial plants has led to conservation issues. Moreover, genetic information on R trisperma is still limited, which is crucial for developmental, physiological, and molecular studies. Studying gene expression is essential to explain plant physiological processes. Nonetheless, this technique requires sensitive and precise measurement of messenger RNA (mRNA). In addition, the presence of internal control genes is important to avoid bias. Therefore, collecting and preserving genetic data for R trisperma is indispensable. In this study, we aimed to evaluate the application of plastid loci, rbcL, and matK, to the DNA barcode of R trisperma for use in conservation programs. In addition, we isolated and cloned the RtActin ( RtACT) gene fragment for use in gene expression studies. Sequence information was analyzed in silico by comparison with other Euphorbiaceae plants. For actin fragment isolation, reverse-transcription polymerase chain reaction was used. Molecular cloning of RtActin was performed using the pTA2 plasmid before sequencing. We successfully isolated and cloned 592 and 840 bp of RtrbcL and RtmatK fragment genes, respectively. The RtrbcL barcoding marker, rather than the RtmatK plastidial marker, provided discriminative molecular phylogenetic data for R Trisperma. We also isolated 986 bp of RtACT gene fragments. Our phylogenetic analysis demonstrated that R trisperma is closely related to the Vernicia fordii Actin gene (97% identity). Our results suggest that RtrbcL could be further developed and used as a barcoding marker for R trisperma. Moreover, the RtACT gene could be further investigated for use in gene expression studies of plant.
Assessment of Blue Carbon Stock of Mangrove Ecosystem in Telok Bangko Bontang N Sumarsih, D Saptarini, D Hidayati, N M Ashuri, D Oktafitria, R I Tawakkal, S Cahya, Y Mulyadi, H D Armono Iop Conference Series Earth and Environmental Science, 2023 Mangroves have high primary productivity so they are known as good carbon storage and absorbers (carbon sequestrators). This ability plays a very important role in the regulation of climate mitigation because the ability of mangrove sequestrators can be utilized to absorb CO2 anthropogenic emissions. Telok Bangko, Loktuan Village, North Bontang, East Kalimantan is an area that has a mangrove ecosystem area, but is also surrounded by industrial areas and settlements. For this reason, this study aimed to assess the mangrove tree species, information on biomass, potential carbon stocks, and CO2 absorption values at that location. Sampling was carried out using the crossed quadrant transect method. At each location the transect was carried out four times. Each transect has four crossed quadrants measuring 10 m x 10 m. In each quadrant, species identification was carried out and then the circumference of the trunk at breast height (Diamter at Breast Height) for mangrove trees (diameter > 4 cm) was measured using a tape measure. Calculation of estimated stored biomass in mangroves using allometric equations using DBH data. Next, estimates of carbon stocks and carbon uptake are calculated. These results will be presented quantitatively descriptive. From this study, 11 species were found, namely Rhizophora mucronata, Avicennia marina, Sonneratia alba, Sonneratia ovata, Derris trifoliata, Ceriops tagal, Lumnitzera racemosa, Xylocarpus granatum, Terminalia catappa, Thespesia populnea, and Wedelia biflora. The results of estimating the carbon stock of the mangrove ecosystem in Bangko Bay were 2236.36 tonnes/ha with a biomass value of 4819.74 tonnes/ha. While in the HGB area, the results of carbon stocks were 2980.48 tonnes/ha with a biomass value of 6423.44 tonnes/ha. Estimated mangrove CO2 absorption in Bangko Bay was 8207.44 tons/ha. Meanwhile, mangrove CO2 absorption in HGB land was 10938.35 tons/ha.
Study of the Use of Block Compos on the Growth of Teak (Tectona grandis) in Used Lands of Kapur Stone Mine Supiana Dian Nurtjahyani, Dwi oktafitria, Sriwulan, Ahmad Zaenal Arifin, Eko Purnomo, Aris Santoso, Ali Mustofa Iop Conference Series Earth and Environmental Science, 2021 The limestone mining area is a karst area that has an important ecological function as a water conservation area. After the mining process, the ex-mining area becomes critical land that is poor in nutrients, decreases soil microbial diversity, increases soil pH and temperature. This study aimed to examine the use of conventional and block compost based on plant height parameters and stem diameter. Block compost was made using the bokashi method with the ingredient of teak leaf litter (Tectona grandis). The composition of leaf litter (30%), manure (40%), and sawdust (30%). Block compost is made by adding adhesive and it is made using a pressing device. Block compost application on plants is very effective compared without block compost. The average plant height with block compost is 163.2 cm, while without block compost is 27 cm. the average of stem growth diameter of plants with block compost of 1.61 cm, while without block compost was 0.71 cm. This shows that block compost is a solution in mining land reclamation.
In vitro shoot micropropagation of Gracilaria verrucosa using plant growth dual regulators Aacl Bioflux, 2021
Short communication: Assessment of reclamation success of former limestone quarries in Tuban, Indonesia, based on soil arthropod diversity and soil organic carbon content DWI OKTAFITRIA, KUNTUM FEBRIYANTININGRUM, NURUL JADID, NIA NURFITRIA, FAHMI RAHMADANI, ALFIAN AMRULLAH, DEWI HIDAYATI Biodiversitas, 2019 Oktafitria D, Febriyantiningrum K, Jadid N, Nurfitria N, Rahmadani F, Amrullah A, Hidayati D. 2019. Assessment of reclamation success of former limestone quarries in Tuban, Indonesia, based on soil arthropod diversity and soil organic carbon content. Biodiversitas 20: 1743-1747. The reclamation for sustainable ecosystem in mined land, including revegetation, is an obligatory program stipulated by the Indonesian government. This study evaluated the ecological improvement of revegetated land at the reclamation sub-areas (RSAs) of former limestone quarries in Tuban, East Java, Indonesia. The RSAs were coded as RSA 2010, RSA 2014, and RSA 2016 which represented the initial year of revegetation as well as the reclamation soil-material, namely the rejected soil-products, topsoil, and spoils, respectively. Soil insects were collected from ten sampling plots at each RSA using pitfall traps. The data of arthropods were analyzed to describe the community structure by determining the Shannon Diversity Index (H’), Simpson’s index (D), Sorensen's similarity and Shannon evenness index (E). The soil organic carbon (SOC) content from the soil composites was analyzed according to the Walkley and Black method. The results showed that SOC value at RSA 2010 (1.851±0.38%) was higher than that at SRA 2014 (1.694±0.29%) and SRA 2016 (0.947±0.10%). There were 18 families found from eight orders of soil insects (Coleoptera, Diplura, Diptera, Entognatha, Hemiptera, Hymenoptera, Neuroptera, and Orthoptera) and one order from terrestrial crustacea i.e., Isopoda, family Tylidae. The ecological indices of RSA 2014 (H’=1.15; D=0.47; E=0.61) and RSA 2016 (H’=1,19; D=0.47; E=0.57) were higher than those of RSA 2010 (H’=0,61; D=0.71; E=0.43). The Pearson analysis indicated moderate negative correlation (r=0.55) between soil arthropod diversity index and SOC. The diversity of soil arthropods at reclaimed area indicated that the reclamation was successful. Based on the study, we recommend that reclamation is done using mine spoil or topsoil rather than rejected soil products.
Short communication: Evaluation of water quality and survival rate of red tilapia (Oreochromis Niloticus) by using rice-fish culture system in quarry land of clay DEWI HIDAYATI, SUPIANA DIAN NURTJAHYANI, DWI OKTAFITRIA, NOVA MAULIDINA ASHURI, WAHYU KURNIALLAH Biodiversitas, 2019 Hidayati D, Nurtjahyani SD, Oktafitria D, Ashuri NM, Kurniallah W. 2019. Short Communication: Evaluation of water quality and survival rate of red tilapia (Oreochromis niloticus) by using rice-fish culture system in quarry land of clay. Biodiversitas 20: 589-594. Quarry land is an area that has been used as mining land and has different characteristics from the former conditions before being mined so that the level of productivity of the soil decreases. This study aims to determine the suitability of clay quarry land in Tuban as a cultivation medium based on the analysis of the water quality and survival rate of red tilapia (Oreochromis niloticus) using rice-fish culture system. This study used a completely randomized experimental design method with triplicate of three ratio of cultivation medium using Ex-mining Land Soil (EmLS) from quarry land and Top Soil, i.e., 1:0 (A), 1:1 (B), and 0:1 (C/Control). Results of water quality measurements that include nitrite, nitrate, temperature, pH, and dissolved oxygen in descriptive unreal influence showed good condition. However, ammonia gives a significantly different effect mainly on the survival rate in 30 days nurture. In the end, the ANOVA test result of tilapia survival in treatment A was not significantly different (P>0.05) with treatment B and control. Based on these data, it can be seen that the EmLS from the quarry land of clay and TS can be used as a medium for cultivation of red tilapia (O. niloticus) with the recommended ratio of 1:1.
Local community knowledge and participation for animal diversity conservation in SSWP IV Sidoarjo, East Java, Indonesia Nova Maulidina Ashuri, Dwi Oktafitria, Indra Wirawan, Zainul Muttaqin, M. Ulya Alfarisy, Abdul Azis, Sherly Eka Argiyanti, Via Nur Fadilah Aip Conference Proceedings, 2017 Key to animal biodiversity conservation are the local communities that live in and around these sites as their livelihoods depend on the natural resources these sites provide. SSWP (Sub Satuan Wilayah Pembangunan) IV Sidoarjo covers Krian, Balongbendo, Tarik, Prambon, and Wonoayu subdistrict with the main function as technical agricultural, industrial zones supported by the low density of settlement activity. Development in this region which tend not balanced between technical agricultural and industrial activities, it is necessary to study in depth so that rapid industrial development can still pay attention to the environment because there is a trend change in agricultural land use and settlements for industrial activities. Take a look at the projections of potential future threats and potential huge biodiversity in SSWP IV is necessary to do a program with a strategic approach to community support efforts to efficiently manage potential biodiversity. As well as the development and diversification of fo...
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