Cell Biology, Biotechnology, Molecular Biology, Molecular Medicine
5
Scopus Publications
Scopus Publications
Comparative effects of MKARE® eggshell membrane and hydrolyzed collagen as nutricosmetics on skin biophysical properties: a randomized clinical trial Yaiza González-Rodríguez, Manuel A. La Nuez-García, Marina Jiménez, Vega Villar-Suárez, Alejandro Casado-Santos Frontiers in Nutrition, 2026 Introduction This study aimed to investigate the impact of fresh eggshell membrane (MKARE®) as a nutricosmetic ingredient, with a focus on its influence on skin properties. Biomechanical measurement devices were used to assess the effects on skin condition, and the results were compared with hydrolyzed collagen and a placebo. Methods Biophysical parameters were analyzed (using Cutometer®, Tewameter®, Corneometer®, and VISIA®) among participants showing signs of aging. These individuals consumed either 300 mg of fresh eggshell membrane (MKARE®) or 8,000 mg of hydrolyzed collagen, and the results were compared to a placebo group after 28 and 57 days. Results The results showed significant improvements in skin firmness/flexibility and elasticity after 57 days of MKARE® intake, compared to both the hydrolyzed collagen and placebo groups. Conclusion This study demonstrates that a 300 mg intake of fresh membrane MKARE® positively affects mechanical skin parameters compared to the group that consumed 8,000 mg of hydrolyzed collagen or the placebo group. These improvements suggest that MKARE® consumption regenerates the skin structure, primarily due to positive changes in collagen and elastin formation, which are key contributors to the improvement of skin firmness, elasticity, and barrier protection.
Optimizing mesenchymal stromal cells priming strategies for tailored effects on the secretome Yaiza González-Rodríguez, Alejandro Casado-Santos, Elsa González-Cubero, Maria Luisa González-Fernández, Alba Sellés-Egea, Vega Villar-Suárez Biomedicine and Pharmacotherapy, 2025 Mesenchymal stromal cells (MSCs) have emerged as a promising tool in regenerative medicine, with recent focus shifting towards their secretome as a cell-free therapeutic approach. This study investigates the impact of various priming strategies on the immunomodulatory, anti-inflammatory, and regenerative potential of adipose-derived MSCs (ASCs) secretomes. We evaluated the effects of hypoxia, pro-inflammatory cytokines, and spheroid culture conditions on ASC secretome composition and functionality. Gene expression analysis, nanoparticle tracking, protein quantification, and functional assays were performed to characterize the secretomes. RNA sequencing revealed significant differences in gene expression profiles across priming conditions, particularly in pathways related to osteogenesis, angiogenesis, inflammation, and neurotrophic factors. Notably, spheroid culture combined with hypoxia and inflammation resulted in a substantial increase in extracellular vesicle production and altered protein content. Functional assays demonstrated enhanced neutrophil inhibition by secretomes from hypoxia-primed ASCs. Our findings indicate that tailored priming strategies can significantly modulate the therapeutic properties of ASC secretomes, potentially enhancing their efficacy in various clinical applications. This study provides valuable insights for optimizing cell-free therapies in regenerative medicine and offers a basis for developing more targeted and effective treatments.
The therapeutic potential of mesenchymal stromal cell secretome in treating spontaneous chronic corneal epithelial defects in dogs Alejandro Casado-Santos, Elsa González-Cubero, Mª. Belén García-Rodríguez, Álvaro Carrera-Serna, Mª Luisa González-Fernández, Vega Villar-Suárez Research in Veterinary Science, 2025 Corneal ulcers in dogs pose a significant challenge in veterinary ophthalmology, often leading to prolonged visual impairment and discomfort. This study aimed to assess the efficacy of adipose tissue-derived mesenchymal stromal cell (ASCs) secretome as a treatment for complicated corneal ulcers in dogs. Ten dogs with spontaneous chronic corneal epithelial defects, were treated with topical application of ASC secretome eye drops. Our results showed that secretome therapy facilitated complete healing of all corneal ulcers within 4 weeks, with an average healing time of 1.2 weeks. Notably, secretome treatment was effective even in cases that had previously failed to respond to conventional therapies. Clinical signs such as blepharospasm, conjunctival hyperemia, and photophobia were alleviated promptly following secretome administration. Secretome therapy was well-tolerated, with no adverse reactions reported, further supporting its safety profile. The findings suggest that ASC secretome represents a promising cell-free and minimally invasive therapeutic approach for the treatment of complicated corneal ulcers in dogs. • ASC secretome represents a promising cell-free therapeutic approach for complicated corneal ulcers in dogs. • ASC secretome facilitated complete healing of complicated corneal ulcers in dogs within 2 weeks. • Secretome therapy was effective even in cases refractory to conventional treatments. • Clinical signs like blepharospasm and conjunctival hyperemia were promptly alleviated after secretome administration. • Secretome treatment demonstrated a favorable safety profile, with no adverse reactions reported.
Equine Corneal Wound Healing Using Mesenchymal Stem Cell Secretome: Case Report Alejandro Casado-Santos, Elsa González-Cubero, Maria Luisa González-Fernández, Yaiza González-Rodríguez, Mª Belén García-Rodríguez, Vega Villar-Suárez Animals, 2024 Corneal ulcers are a common and potentially vision-threatening condition in horses that can be challenging to treat with conventional therapies alone. This case report describes the successful treatment of a non-healing corneal ulcer in a 28-year-old Hispano-Bretón mare using the secretome derived from adipose tissue-derived mesenchymal stem cells (ASCs). Despite initial treatment with antibiotics, anti-inflammatory drugs, and surgical debridement, the corneal ulcer failed to heal properly, exhibiting persistent epithelial defects and stromal complications. As an alternative regenerative approach, the ASC secretome, a rich source of trophic factors, cytokines, and extracellular vesicles, was topically administered to the affected eye. Remarkably, within one week of secretome treatment, the clinical signs of blepharospasm and epiphora resolved, and the corneal ulcer exhibited complete re-epithelialization, regained transparency, and reduced neovascularization. No recurrence was observed during the 1.5-year follow-up period. This case highlights the potential therapeutic benefits of the ASC secretome in promoting corneal wound healing and suggests its promise as a novel cell-free therapy for treating refractory corneal ulcers in horses.
Anti-inflammatory and regenerative effects of MKARE® Eggshell Membrane: An in vitro osteoarthritis model and placebo-controlled clinical study Alejandro Casado-Santos, Manuel A. La Nuez-García, Patricia Álvarez-Rodríguez, Elsa González-Cubero, Yaiza González-Rodríguez, Maria Luisa González-Fernández, Vega Villar-Suárez Journal of Functional Foods, 2024 MKARE®, a 100% natural ingredient derived from fresh eggshell membrane (ESM), has a rich composition in bioactive compounds like collagen, hyaluronic acid, and elastin. These components are beneficial for managing osteoarthritis (OA) due to their anti-inflammatory and regenerative properties. Highlighting the significance of freshness, our research has shown that the effectiveness of MKARE® is higher than that of other commercial products based on ESM that have been stored for several days at room temperature, losing their bioactive compounds. This study explores the MKARE® anti-inflammatory capacity through an in vitro and clinical analyses, demonstrating its ability to alleviate OA symptoms and improve joint health. This underscores the crucial role of freshness in optimizing the therapeutic benefits.
