Holds a full degree in Biological Sciences from the State University of Piauí (2005), a Master's degree in Botany from the Federal University of Viçosa (2008), and a Ph.D. in Botany from the Federal University of Viçosa (2014). Currently, she is an Associate II Professor at the State University of Piauí. She is a permanent faculty member of the Professional Master's Program in Biology Teaching (PROFBIO) at the Federal University of Minas Gerais, working through the State University of Piauí. She has experience in the field of Botany, with an emphasis on Plant Anatomy, mainly working on the following themes: Byrsonima, Heteropterys, Banisteriopsis, taxonomic anatomy, Stigmaphylloideae, and Malpighiaceae.
Leaf anatomy of species of Calea sect. Lemmatium (Asteraceae) with a taxonomic emphasis Maria José de Sousa Monteiro, Marcos Paulo Silva Paz, Genilson Alves dos Reis e Silva, Josiane Silva Araújo Nordic Journal of Botany, 2025 Calea sect. Lemmatium is one of five sections of Calea (Neurolaeneae, Asteraceae), containing 12 species that are morphologically similar, which makes their identification difficult to non‐experts. The current study aimed to provide an anatomical characterization of species from Calea sect. Lemmatium to support their taxonomy and contribute to the distinction and delimitation of the sections of Calea. Anatomical material was obtained from herbarium collections, re‐hydrated, sectioned by hand, clarified in 50% sodium hypochlorite, and stained with Astra blue and basic fuchsin. Characters considered relevant were petiole contour, trichome type, stomate type, petiole and midrib vascular system organization, sclereids, laticifers and cavities, druses and crystals, isobilateral and dorsiventral mesophyll, and parenchymatous and sclerenchymatous sheath extension. Additionally, sclereids and laticifers are described for the first time in Calea and tribe Neurolaeneae. Thus, our study provides support to the taxonomy of the genus and can provide information to future taxonomic studies in Asteraceae.
Leaf anatomy of seven endemic species of Glicophyllum (Malpighiaceae) in Brazil CATARINA S. LIMA, JULIANO DA SILVA LIMA, FRANCISCO S. SANTOS-FILHO, JOSIANE S. ARAÚJO Anais Da Academia Brasileira De Ciencias, 2024 Glicophyllum is well supported, presenting four homoplasies, easily differentiated from the other genera of the clade due to characteristics related to the reproductive organs, which can make the identification of their species difficult when they are not in the reproductive phase. Therefore, there are provided the leaf anatomical and morphological description of the leaf glands of seven species of Glicophyllum to assist in the identification of their species. The samples for the study were obtained from several national and international herbaria, sectioned freehand, stained with basic fuchsin - astra blue and compared through a binary matrix using the Sorensen's coefficient in the MVSP software. Among the leaf anatomical characters found, the following stand out: presence/absence of trichomes; petiole contour; contour of the main vein; organization of the mesophyll, presence/absence of bundle sheath extension and the surface of the glands. In this study, an identification key with leaf anatomical data is presented for the first time, demonstrating the applicability of leaf anatomy for the taxonomy of Glicophyllum. In the multivariate analysis, it is observed that the characteristics of leaf venation and topology of the glands are more representative to differentiate the taxa. Therefore, the data obtained can support future taxonomic and phylogenetic studies of the genus.
Understanding the anatomical diversity of leaves in the Aspicarpoid clade: New insights for Malpighiaceae systematics MILENA PEREIRA VILARINHO, ANTONIA RITA DE MORAES CÂMARA, JOÃO VICTOR DA COSTA SANTOS, JOSIANE SILVA ARAÚJO Phytotaxa, 2024 The Aspicarpoid clade represents a strongly supported group within the Stigmaphylloid lineage. The group is composed of four neotropical genera: Aspicarpa Rich., Gaudichaudia Kunth, Janusia A.Juss. and Peregrina W.R.Anderson. In this study, we studied the utility of leaf anatomy characters to support groups recovered in molecular phylogenies and define synapomorphies that can be used in future infra- and supra-generic classifications. Camarea axillaris A.St.-Hil. (1823: 10) and Cottsia Dubard & Dop (1908: 20) were used as outgroups for comparison. Dry leaf samples were re-hydrated, sectioned, stained, and analyzed using a microscope and a stereomicroscope. Characters such as presence and absence of glands, their location and morphotype, petiole and midrib contour, presence of trichomes, accessory bundles, vascular system organization and mesophyll type were utilized for species comparison, showing high taxonomic value for the group. Leaf glands, accessory bundles, sheath extension in the mesophyll and presence of druses and prismatic crystals were the most valuable characters in an evolutionary approach for the clade. Our work confirms the power of leaf anatomy as a taxonomic and evolutionary tool in Malpighiaceae Juss. (1789: 252), as well as its contribution to the delimitation of phylogenetic groups in the Aspicarpoid clade.
Contributions of leaf morphoanatomy to the taxonomy of Bronwenia (Malpighiaceae Juss.) MILENA PEREIRA VILARINHO, JOÃO VICTOR DA COSTA SANTOS, FRANCISCO SOARES SANTOS-FILHO, JOSIANE SILVA ARAÚJO Phytotaxa, 2023 Bronwenia has about 11 species whose current delimitation is the result of a complicated taxonomic history marked by recent evolutionary hypotheses in Malpighiaceae. Therefore, there is a need for approaches that contribute to circumscribing the group and distinguishing the species. Thus, the aim of this work was to prove the effectiveness of leaf anatomy in this purpose and point out the characters that distinguish the species of Bronwenia. Plant anatomy techniques were used and the coded anatomical characters were plotted in a binary matrix and interpreted in a cluster analysis, using UPGMA. The relevant characters to distinguish the taxa were: petiole contour, petiole/midrib vascular system conformation, presence or absence malpighiaceous trichomes and laticifers. While the remaining characters occur widely in the group and can be used in future approaches to distinguish Bronwenia from other related genera, including Diplopterys and Banisteriopsis, which previously constituted a single genus. Therefore, leaf anatomy represents relevance in the taxonomy of Bronwenia and can direct evolutionary hypotheses for Malpighiaceae.
Morphoanatomy of the leaf and calicinal glands of Stigmaphyllon A.Juss. (Malpighiaceae): Functional evidence, taxonomic and evolutionary contributions Rafael Ribeiro de Matos, Josiane Silva Araújo Hoehnea, 2021 RESUMO A morfoanatomia das glândulas foliares e calicinais de Stigmaphyllon A.Juss. ocorrentes no Brasil foi realizada a partir de folhas expandidas coletadas e fixadas no campo ou de espécimes de herbário, processadas e analisadas por métodos anatômicos usuais. Os caracteres variáveis registrados neste estudo foram relacionados à posição das glândulas na lâmina foliar e pecíolo, forma, presença/ausência de pedúnculos nas glândulas, presença/ausência de cutícula e espaço subcuticular, epiderme em paliçada e padrões de vascularização. As glândulas foliares e do cálice em Stigmaphyllon são estruturalmente semelhantes, corroborando estudos anatômicos anteriores na família e reforçando a hipótese de que as glândulas calicinais podem ser evolutivamente derivadas de glândulas foliares em Malpighiaceae.
The taxonomic value of leaf anatomy for species byrsonima: A difficult genus of malpighiaceae juss. João Victor da Costa Santos, Maria de Fátima Vasconcelos de Oliveira, Francisco Soares Santos Filho, Leyde Nayane Nunes dos Santos Silva, Josiane Silva Araújo Acta Botanica Brasilica, 2020 We studied the leaf anatomy of 15 species of Byrsonima and a single species of Diacidia as an outgroup to test the relevance of leaf anatomical characters to the complicated taxonomy of this genus. We present complete anatomical descriptions for petioles and leaf blades, together with an anatomical matrix, dendrogram and an identification key for the studied taxa. The most informative characters were the presence of stomatal crest, trichome type, epidermis type, contour and conformation of the vascular system in the diaphragm and medium petiole and number of accessory bundles. The aforementioned characters revealed that the anatomy of the petioles in the genus differs from that of petioles in the outgroup taxon with regard to vascular system conformation and the absence of cortical sclereids. Furthermore, the anatomy of the leaf blade appears to be quite informative in Byrsonima, especially in synonymized species. The anatomical features evaluated here have great value for Byrsonima taxonomy. These features may be used in an evolutionary approach to the group, especially for proposing a new classification system based on morphoanatomical characters of the genus.
New records of Aristolochia bahiensis F.Gonzalez for the state of Maranhão, Brazil Lorran André Moraes, Josiane Silva Araújo, Francisco Soares Santos-Filho Revista Brasileira De Geografia Fisica, 2020 This study aimed to record the occurrence of the species Aristolochia bahiensis F. González in an area of the territory of the State of Maranhão, Brazil. The species was collected in the city of Timon, in an area of tension that brings together characteristics of the Cerrado, adjacent to Mata Ciliar. The study provides a brief characterization of the botanical family and descriptions of the genus and species, as well as data on its distribution. The species had already been found in the states of Alagoas, Bahia and Espírito Santo. In addition to portraying the geographic occurrence of the plant, a morphological characterization of the species was made, with the assembly of a board with images of the most relevant aspects of its morphology in addition to maps with its location. Novo registro de Aristolochia bahiensis F.Gonzalez para o Estado do Maranhão, Brasil R E S U M OEste trabalho teve como objetivo registrar a ocorrência da espécie Aristolochia bahiensis F. González em área do território do Estado do Maranhão, Brasil. A espécie foi coletada na cidade de Timon, em área de tensão que reúne características de Cerrado, em adjacência com Mata Ciliar. O estudo traz uma breve caracterização da família botânica e descrições sobre o gênero e a espécie, além de dados sobre sua distribuição. A espécie já tinha sido encontrada nos estados de Alagoas, Bahia e Espírito Santo. Além de retratar ocorrência geográfica da planta foi feita uma caracterização morfológica da espécie, com a montagem de uma prancha com imagens dos aspectos mais relevantes da sua morfologia além de mapas com sua localização.Palavras-chave: Aristolochiaceae, Aristolochia, Aristolochia bahiensis, Área de Tensão Ecológica, Cerrado, Mata Ciliar, Bacia do Rio Parnaíba, Nordeste Brasil.
Taxonomic relevance of leaf anatomy in Banisteriopsis C.B. Rob. (Malpighiaceae) Josiane Silva Araújo, Rafael Felipe de Almeida, Renata Maria Strozi Alves Meira Acta Botanica Brasilica, 2020 We describe the leaf anatomy of 42 of the 60 species of the genus Banisteriopsis, one of the largest and most diverse genera of Malpighiaceae in the Cerrado domain. Our aim was to identify anatomical leaf characters useful for the taxonomy and systematics of this morphological diverse genus. Expanded leaves from herbarium specimens were rehydrated and processed according to usual anatomical methods for analysis under light microscopy. All characters were compared using the Unweighted Pair Group Method with Arithmetic Mean algorithm and expressed in dendrograms. The most useful leaf anatomical characters were petiole contour, presence/absence of vascular bundles in the petiole, the position of the vascular bundles in the petiole, shape of the midrib, presence/absence of sheath extension, mesophyll type, and both the presence/absence and shape of leaf glands. Our study indicates that the morphological groups proposed in the literature for Banisteriopsis are natural groups.
