In silico analysis of Chlorophytum borivilianum phytocompounds as a multi-targeted directed ligand (MTDL) against type 2 diabetes mellitus-induced Alzheimer’s disease Kanika Khoba, Shivani Kumar, Ram Singh Purty Natural Product Research, 2025 Alzheimer’s disease (AD) is a multi-factorial neurodegenerative disorder. A crosstalk between AD and hyperglycaemia has been observed where patients showed increased insulin resistance. Type 2 diabetes mellitus and its features, including hyperinsulinemia and chronic hyperglycaemia with an inflammatory response, are related to AD through insulin resistance. Here, aim was to identify a phytocompound that could be used as a potential drug candidate. GC-MS analysis of Chlorophytum borivilianum root extract provided a phytocompound library, of which 10 phytocompounds were selected for docking. Protein targets, namely acetylcholinesterase, butrylcholinesterase, Amyloid-β protein, BACE-1, N-methyl-D-aspartate, GSK-3β, APAF-1 and caspase-3, were used for docking. Based on pharmacokinetic and ADMET analysis, 25-Homo-24-Ketocholesterol, showed the lowest binding affinity with the targets, justified Lipinski’s rule of 5, had high potency, good absorption and was less toxic to the human body. We conclude that 25-homo-24-ketocholesterol may act as a potential drug with multi-target directed ligand activity.
Design of a Bio-Sorption Tower for Reuse of Agricultural Run-off Sudeep Asthana, Swapna Rekha Panda, Dishali Asrani, Ram Singh Purty, Sayan Chatterjee Aip Conference Proceedings, 2024 Heavy metal contamination of water has been an issue of great concern since a long time. The water resources have been depleting rapidly and this is not an unknown fact to mankind. Water shortages have become a consistent problem faced by mankind. Every now and then we hear news about contamination of water bodies by industrial effluents or sewage water. Increased agricultural activities, industrial ventures and poor e-waste management are few of the key reasons that are accountable for abnormal rise in the heavy metal concentration in water bodies. Many methods have been devised to purify wastewater but the use of biosorptive materials has gained popularity recently. The techniques like membrane filtration, ion-exchange, carbon adsorption, co-precipitation or adsorption, electrolysis and chemical precipitation are made use of for the elimination of heavy metals from wastewater, but these processes are appropriate for the removal of elevated concentration of heavy metals and are also very expensive. These techniques are not very functional when it comes to the removal of low concentration of metals. This is the drawback that creates scope for using cheap bio sorbents/adsorbents for heavy metal removal from wastewaters. Thus a bio sorption tower for reuse and recycle of agricultural run-off and e-waste was developed using a novel multilayer packed bed. The packed bed was designed by exploring the potential of various inexpensive bio sorbents by using them without any pre-treatment. Lab-scale run of the bio sorption tower thus designed and run with synthetic agricultural waste water showed good potential for bio sorption of heavy metals.
Process Design for a Low Cost Portable Green Filter for Water Purification Sayan Chatterjee, Ram Singh Purty, Dishali Asrani, Prashant Kumar, Swapna Rekha Panda, Sudeep Asthana Aip Conference Proceedings, 2024 Water is a vital resource and one of the major causes of existence of life on earth. There has been no sustainable re-use of this resource and we are on the verge of running out of this resource in no time. The removal of metals and other potential contaminants from used water for reuse needs a validated large-scale cost-effective method. The development of a novel biofilter was aimed in this project with the bio-sorbant as spent tea leaves. The various parameters of the biofiltration processes, their mechanism for heavy metals removal along with the efficiency of the biofilters and its scale up aspects have been studied. The work was targeted towards removal of Nickel (Ni) and Cadmium (Cd), two very common contaminant in urban and rural ground water as well as in industrial spent waters. In a proof-of-concept study, a laboratory-scale closed biofilter system employing the trickle bed process was operated to remove Nickel (Ni) and Cadmium (Cd) from a synthetic waste water sample at a load of 1000 mg/L of the heavy metal. Spent tea leaves after proper pre-treatment was used as filter media. Depth profile analysis of the filter bed showed the reduction of a steep gradient of Nickel (Ni) and Cadmium (Cd) from the top layer to the bottom layer of filter media in the biofilter. Nickel (Ni) and Cadmium (Cd) level at the bottom of the biofilter decreased over 78% over a period of 6-8 hours of experiment. With these observations we have scaled up the process from a 0.050 L to 15.0 L operating volume. The results were comparable in the scaled up set up.
Successful fertility restoration in male-sterile barnase line by optimal expression of barstar gene for hybrid-rice seed production Pravin Kumar, Ram Singh Purty Journal of Crop Improvement, 2024 The basic requirement for the barnase-barstar-based hybrid-seed production technology is the availability of stable male-sterile barnase and restorer barstar lines. Development of two rice (Oryza sativa) male-sterile barnase lines has been earlier reported from our lab. Complete restoration of male fertility in transgenic male-sterile lines has always been a challenging task for molecular breeders. In the present study, we have reported the successful fertility restoration in male-sterile barnase lines using barstar restorer lines generated through two completely different tapetum-specific promoters. The barstar lines were not able to restore male fertility in one barnase line but did restore it in the other barnase line. The male-sterile barnase line and its corresponding restorer line could be diversified into appropriate combiners for heterosis rice breeding.
Immunogenicity studies on lantibiotics Navjyoti Chakraborty, Aditi Joshi, Khushi Ahuja, Apoorva Vashisht, Adreeja Basu, Ram Singh Purty, Sayan Chatterjee Lantibiotics as Alternative Therapeutics, 2023
Comparative analysis of computational methods used in Protein-Protein Interaction (PPI) studies Computational and Analytic Methods in Biological Sciences Bioinformatics with Machine Learning and Mathematical Modelling, 2022
EXPRESSION ANALYSIS of OsPHD6 GENE in RESPONSE to SALINITY STRESS and REHYDRATION in RICE SEEDLINGS Plant Cell Biotechnology and Molecular Biology, 2020
Presence of heavy metal in water (Yamuna River), soil and vegetables in Delhi and to examine the effect of phyto-accumulating capacity of Eichhornia crassipes Plant Cell Biotechnology and Molecular Biology, 2020
Expression analysis of serine biosynthesis pathway genes under various abiotic stress in Brassica juncea (L.) Czern. Plant Cell Biotechnology and Molecular Biology, 2020
Plant photoreceptors as sensory proteins in driving cell signaling network Y Sarangle, RS Purty Plant Receptors in Cellular Signaling, 251-276 , 2026 2026
Histidine kinase receptors and two-component signaling in driving developmental and environmental stress responses in plants D Vaish, R Vyas, RS Purty Plant Receptors in Cellular Signaling, 31-42 , 2026 2026
Analysis of endogenous acetylcholine and its correlation with expression of AChE-like genes in tomato (Solanum lycopersicum) Y Sarangle, K Saha, RS Purty, K Bamel Plant Physiology Reports , 2025 2025
In silico analysis of Chlorophytum borivilianum phytocompounds as a multi-targeted directed ligand (MTDL) against type 2 diabetes mellitus-induced Alzheimer’s … K Khoba, S Kumar, RS Purty Natural Product Research, 1-7 , 2025 2025
Accumulation of γ-sitosterol in response to drought stress and its interaction with plant stress signaling receptors in Brassica juncea K Khoba, RS Purty Journal of Plant Biochemistry and Biotechnology , 2024 2024 Citations: 2
Molecular Docking Analysis of Plant Receptors with Different Forms of Rhizobacterial Compounds A Sharma, RS Purty Rhizospheric Interactions for Abiotic Stress Mitigation, 329-342 , 2024 2024
Utility and Applications of Biofertilizers for Enhanced Crop Productivity and Sustainable Agriculture S Chauhan, RS Purty Rhizospheric Interactions for Abiotic Stress Mitigation, 145-155 , 2024 2024
Role of rhizosphere microbial community in mitigation of abiotic stresses in plants D Vaish, RS Purty Plant-Microbial Interactions for Sustainable Agriculture, 179 , 2024 2024
Unraveling the impact of abiotic stress on conserved microRNA expression and their target genes in Stevia rebaudiana B Anjna, RS Purty Physiology and Molecular Biology of Plants 30 (11), 1795-1818 , 2024 2024 Citations: 1
In silico approach for evaluating the degradation efficiency of plastic degrading enzyme mono(2-hydroxyethyl) terephthalic acid hydrolase (MHETase) of selected … T Watts, K Khoba, RS Purty Bioremediation Journal 28 (4), 541-552 , 2024 2024 Citations: 5
Role of acetylcholine and acetylcholinesterase in improving abiotic stress resistance/tolerance Y Sarangle, K Bamel, RS Purty Communicative & Integrative Biology 17 (1), 2353200 , 2024 2024 Citations: 15
Computational drug repositioning of mast cell stabilizers against human Protease-Activated Receptor 2 (PAR2) involved in Rheumatoid Arthritis K Choudhury, K Khoba, RS Purty MDPI in The 3rd International Electronic Conference on Biomolecules session … , 2024 2024
Computational inhibition of S100A8 (calgranulin A) as a potential non-invasive biomarker for rheumatoid arthritis K Choudhury, A Khadanga, RS Purty In Silico Pharmacology 12 (1), 25 , 2024 2024 Citations: 5
Identification of Acetylcholinesterase Like Gene Family and Its Expression Under Salinity Stress in Solanum lycopersicum Y Sarangle, K Bamel, RS Purty Journal of Plant Growth Regulation 43 (3), 940-960 , 2024 2024 Citations: 8
Design of a bio-sorption tower for reuse of agricultural run-off S Asthana, SR Panda, D Asrani, RS Purty, S Chatterjee AIP Conference Proceedings 2986 (1), 030186 , 2024 2024
Process design for a low cost portable green filter for water purification S Chatterjee, RS Purty, D Asrani, P Kumar, SR Panda, S Asthana AIP Conference Proceedings 2986 (1), 030187 , 2024 2024 Citations: 1
Comparative Analysis of Computational K Ahuja, A Joshi, N Chakraborty, RS Purty Computational and Analytic Methods in Biological Sciences: Bioinformatics … , 2023 2023
Comparative Analysis of Computational Methods used in Protein-Protein Interaction (PPI) Studies K Ahuja, A Joshi, N Chakraborty, RS Purty, S Chatterjee Computational and Analytic Methods in Biological Sciences, 63-100 , 2023 2023 Citations: 2
Isolation, Characterization, and In Silico Interaction Studies of Bioactive Compounds from Caesalpinia bonducella with Target Proteins Involved in Alzheimer’s … K Khoba, S Kumar, S Chatterjee, RS Purty Applied Biochemistry and Biotechnology 195 (4), 2216-2234 , 2023 2023 Citations: 10
Immunogenicity studies on lantibiotics N Chakraborty, A Joshi, K Ahuja, A Vashisht, RS Basu, Adreeja, Purty, ... Lantibiotics as Alternative Therapeutics 1, 256-275 , 2023 2023 Citations: 2
MOST CITED SCHOLAR PUBLICATIONS
Physiological responses among Brassica species under salinity stress show strong correlation with transcript abundance for SOS pathway-related genes G Kumar, RS Purty, MP Sharma, SL Singla-Pareek, A Pareek Journal of plant physiology 166 (5), 507-520 , 2009 2009 Citations: 183
Towards salinity tolerance in Brassica : an overview RS Purty, G Kumar, SL Singla-Pareek, A Pareek Physiology and Molecular Biology of Plants 14 (1), 39-49 , 2008 2008 Citations: 155
Saltol QTL and Their Role in Salinity Tolerance in Rice A Waziri, P Kumar, RS Purty Austin Journal of Biotechnology & Bioengineering 3 (3), 1067 , 2016 2016 Citations: 98
DNA Barcoding: An Effective Technique in Molecular Taxonomy RS Purty, S Chatterjee Austin Journal of Biotechnology & Bioengineering 3 (1), 1059. , 2016 2016 Citations: 94
Transformation of tomato cultivar ‘Pusa Ruby’ with bspA gene from Populus tremula for drought tolerance R Roy, RS Purty, V Agrawal, SC Gupta Plant Cell, Tissue and Organ Culture 84 (1), 56-68 , 2006 2006 Citations: 93
Optimization of De Novo Short Read Assembly of Seabuckthorn ( Hippophae rhamnoides L.) Transcriptome R Ghangal, S Chaudhary, M Jain, RS Purty, P Chand Sharma PLoS One 8 (8), e72516 , 2013 2013 Citations: 49
Assessment of Bioremediation Potential of Cellulosimicrobium sp. for Treatment of Multiple Heavy Metals T Bhati, R Gupta, N Yadav, R Singh, A Fuloria, A Waziri, S Chatterjee, ... Microbiology and Biotechnology Letters 47 (2), 269-277 , 2019 2019 Citations: 37
In silico identification and validation of miRNA and their DIR specific targets in Oryza sativa Indica under abiotic stress DK Singh, S Mehra, S Chatterjee, RS Purty Non-coding RNA Research 5 (4), 167-177 , 2020 2020 Citations: 35
Downregulation of candidate gene expression and neuroprotection by Piperine in Streptozotocin-induced Hyperglycemia and Memory Impairment in Rat S Kumar, S Chowdhury, A Razdan, D Kumari, RS Purty, H Ram, P Kumar, ... Frontiers of Pharmacology, doi: 10.3389/fphar.2020.595471 , 2021 2021 Citations: 31
Cloning, structural and expression analysis of OsSOS2 in contrasting cultivars of rice under salinity stress R Purty 2012 Citations: 25
Maintenance of stress related transcripts in tolerant cultivar at a level higher than sensitive one appears to be a conserved salinity response among plants G Kumar, RS Purty, SL Singla-Pareek, A Pareek Plant Signaling & Behavior 4 (5), 431-434 , 2009 2009 Citations: 23
COVID‑19 pandemic: Understanding the emergence, pathogenesis and containment MK Parvez, RM Jagirdar, RS Purty, SK Venkata, V Agrawal, J Kumar, ... World Academy of Sciences Journal 2 (5), 18 , 2020 2020 Citations: 16
Role of acetylcholine and acetylcholinesterase in improving abiotic stress resistance/tolerance Y Sarangle, K Bamel, RS Purty Communicative & Integrative Biology 17 (1), 2353200 , 2024 2024 Citations: 15
Induction of a novel boiling stable protein in response to desiccation and ABA treatments in Sesbania sesban var. bicolor leaves RS Purty, V Agrawal, SC Gupta Biologia plantarum 49 (1), 137-140 , 2005 2005 Citations: 14
Structural and Expression Analysis of Salinity Stress Responsive Phosphoserine Phosphatase from Brassica juncea (L.) RS Purty, M Sachar, S Chatterjee Journal of Proteomics & Bioinformatics 10 (4), 119-127 , 2017 2017 Citations: 13
Isolation, Characterization, and In Silico Interaction Studies of Bioactive Compounds from Caesalpinia bonducella with Target Proteins Involved in Alzheimer’s … K Khoba, S Kumar, S Chatterjee, RS Purty Applied Biochemistry and Biotechnology 195 (4), 2216-2234 , 2023 2023 Citations: 10
Genome-wide analysis of PHD finger gene family and identification of potential miRNA and their PHD finger gene specific targets in Oryza sativa indica A Waziri, DK Singh, T Sharma, S Chatterjee, RS Purty Non-coding RNA Research 5 (4), 191-200 , 2020 2020 Citations: 9
Development of male sterile transgenic lines in rice by tapetum specific expression of barnase gene P Kumar, K Kaur, RS Purty, M Mohan, PK Burma Journal of Plant Biotechnology 44 (4), 364-371 , 2017 2017 Citations: 9
Identification of Acetylcholinesterase Like Gene Family and Its Expression Under Salinity Stress in Solanum lycopersicum Y Sarangle, K Bamel, RS Purty Journal of Plant Growth Regulation 43 (3), 940-960 , 2024 2024 Citations: 8
