Surgical Repair of a Mandibular Fracture in an Amazon Parrot (Amazona aestiva) Lucas Rego Ramos, Osmar de Agostinho Neto, Carolina Kaminski Sanz, Cláudia Maria Pereira, Eveliny Eleutério Oliveira, Patrícia Montresor, Cássia Maria Molinaro Coelho, Marcos Fabio DosSantos Journal of Veterinary Dentistry, 2025 Injured birds pose a challenge in veterinary medicine, requiring innovative approaches for restoration of function and enhancing the quality of life. This case report describes the successful reconstruction of the mandibular beak of an Amazona aestiva parrot (Psittacidae) after a traumatic injury. The injury caused a fracture in the mandibular beak, resulting in significant limitation of function. Computed tomography imaging was used for surgical planning, allowing visualization of the remaining mandibular beak on the parrot's rhamphotheca and determination of the appropriate dimensions of the implanted plate. Plates were surgically applied to reconstruct the mandibular beak structure. The surgery followed an Enhanced Recovery After Surgery protocol, which resulted in enhanced mandibular beak function and total adjustment to the plates. Six months posttreatment, the beak components had fused, and the plates had detached. At the 1- and 2.5-year follow-up, veterinarian assessment noted no concerns with chewing or ingesting food. This study highlights the effectiveness of employing surgical techniques derived from dental expertise and utilizing biomaterials, such as human maxillofacial plates, to successfully reconstruct complex structures in birds.
What are the main oral manifestations in heart transplant patients? A scoping review Marianne Corrêa dos Santos Pimentel, Mariana Magalhães Fernandes, Jéssica de Oliveira Vogel, Clara Herrera Freire, Cláudia Maria Pereira, Bruno Augusto Benevenuto de Andrade, Bruna de Oliveira Rech, Jefferson R. Tenório Special Care in Dentistry, 2024 ObjectiveThe purpose of this scoping review is to evaluate the oral manifestations (OM) of heart transplant (HT) patients undergoing immunosuppressive therapy (IT).Material and methodsA literature search was performed using keywords and MeSH terms related to OM and HT in the Medline/PubMed, Web of Science, Cochrane Library, Scopus, LILACS/BBO databases and in gray literature without language or date restrictions until June 2023. Studies that evaluated HT individuals who used any IT and who reported the occurrence of OM were considered eligible. The results from the search were imported to EndNote Web, and duplicates were removed followed by title/abstract and full‐text analysis.ResultsA total of 402 nonduplicated studies were found and 13 fulfilled the criteria and were included in the present review: 10 cross‐sectional, 2 cohorts, and 1 clinical trial. The most reported OM were periodontal diseases, including drug‐induced gingival enlargement (DIGE), gingival bleeding, gingivitis, and periodontitis. Reported in a minority of studies are oral cancer, opportunistic infections (oral hairy leukoplakia and erythematous candidiasis), enamel defects, and burning mouth.ConclusionConsidering the methodological heterogeneity of the studies analyzed, DIGE is the most commonly observed oral manifestation in HT individuals.
CCL21-CCR7 signaling promotes microglia/macrophage recruitment and chemotherapy resistance in glioblastoma Luiz Henrique Geraldo, Celina Garcia, Yunling Xu, Felipe Saceanu Leser, Izabella Grimaldi, Eduardo Sabino de Camargo Magalhães, Joost Dejaegher, Lien Solie, Cláudia Maria Pereira, Ana Helena Correia, Steven De Vleeschouwer, Bertrand Tavitian, Nathalie Henriques Silva Canedo, Thomas Mathivet, Jean-Leon Thomas, Anne Eichmann, Flavia Regina Souza Lima Cellular and Molecular Life Sciences, 2023 Glioblastoma (GBM) is the most common and fatal primary tumor of the central nervous system (CNS) and current treatments have limited success. Chemokine signaling regulates both malignant cells and stromal cells of the tumor microenvironment (TME), constituting a potential therapeutic target against brain cancers. Here, we investigated the C–C chemokine receptor type 7 (CCR7) and the chemokine (C–C-motif) ligand 21 (CCL21) for their expression and function in human GBM and then assessed their therapeutic potential in preclinical mouse GBM models. In GBM patients, CCR7 expression positively associated with a poor survival. CCL21–CCR7 signaling was shown to regulate tumor cell migration and proliferation while also controlling tumor associated microglia/macrophage recruitment and VEGF-A production, thereby controlling vascular dysmorphia. Inhibition of CCL21–CCR7 signaling led to an increased sensitivity to temozolomide-induced tumor cell death. Collectively, our data indicate that drug targeting of CCL21–CCR7 signaling in tumor and TME cells is a therapeutic option against GBM.
Action of Hyaluronic Acid as a Damage-Associated Molecular Pattern Molecule and Its Function on the Treatment of Temporomandibular Disorders Natália dos Reis Ferreira, Carolina Kaminski Sanz, Aline Raybolt, Cláudia Maria Pereira, Marcos Fabio DosSantos Frontiers in Pain Research, 2022 The temporomandibular joint is responsible for fundamental functions. However, mechanical overload or microtraumas can cause temporomandibular disorders (TMD). In addition to external factors, it is known that these conditions are involved in complex biological mechanisms, such as activation of the immune system, activation of the inflammatory process, and degradation of extracellular matrix (ECM) components. The ECM is a non-cellular three-dimensional macromolecular network; its most studied components is hyaluronic acid (HA). HA is naturally found in many tissues, and most of it has a high molecular weight. HA has attributed an essential role in the viscoelastic properties of the synovial fluid and other tissues. Additionally, it has been shown that HA molecules can contribute to other mechanisms in the processes of injury and healing. It has been speculated that the degradation product of high molecular weight HA in healthy tissues during injury, a low molecular weight HA, may act as damage-associated molecular patterns (DAMPs). DAMPs are multifunctional and structurally diverse molecules that play critical intracellular roles in the absence of injury or infection. However, after cellular damage or stress, these molecules promote the activation of the immune response. Fragments from the degradation of HA can also act as immune response activators. Low molecular weight HA would have the ability to act as a pro-inflammatory marker, promoting the activation and maturation of dendritic cells, the release of pro-inflammatory cytokines such as interleukin 1 beta (IL-1β), and tumor necrosis factor α (TNF-α). It also increases the expression of chemokines and cell proliferation. Many of the pro-inflammatory effects of low molecular weight HA are attributed to its interactions with the activation of toll-like receptors (TLRs 2 and 4). In contrast, the high molecular weight HA found in healthy tissues would act as an anti-inflammatory, inhibiting cell growth and differentiation, decreasing the production of inflammatory cytokines, and reducing phagocytosis by macrophages. These anti-inflammatory effects are mainly attributed to the interaction of high-weight HA with the CD44 receptor. In this study, we review the action of the HA as a DAMP and its functions on pain control, more specifically in orofacial origin (e.g., TMD).
Saliva NMR-Based Metabolomics in the War against COVID-19 Gilson Costa dos Santos Junior, Claudia Maria Pereira, Tatiana Kelly da Silva Fidalgo, Ana Paula Valente Analytical Chemistry, 2020 COVID-19 is an emergent, worldwide public health concern. Joint efforts have been made by scientific communities of various fields to better understand the mechanisms of action of SARS-CoV-2. The need to understand the pathophysiological fingerprint and pathways of this disease make metabolomics-related approaches an indispensable tool for properly answering concerns relating to disease course. Determination of the metabolomic profile may help to explain the heterogeneous spectra of COVID-19 clinical phenotypes and be useful in monitoring disease progression as well as therapeutic treatments. In this sense, saliva has proven to be a strategic biofluid, owing not only to its appeal as a noninvasive sampling method but also due to the capacity of the virus to invade epithelial cells of the oral mucosa and salivary gland ducts via ACE2 receptors. Accordingly, important changes in metabolism have been described relating to COVID-19, indicating that metabolomics may open new avenues for understanding the pathophysiology of this disease, especially via longitudinal study designs. Thus, we discuss the importance of comprehending the SARS-CoV-2 salivary metabolomic fingerprint and also highlight the situation of Brazil on the frontlines of the war against COVID-19.
Neuromechanisms of SARS-CoV-2: A Review Marcos F. DosSantos, Sylvie Devalle, Veronica Aran, Daniela Capra, Natália Roberta Roque, Juliana de Mattos Coelho-Aguiar, Tânia Cristina Leite de Sampaio e Spohr, Janice Gonçalves Subilhaga, Cláudia Maria Pereira, Isabella D'Andrea Meira, Paulo Niemeyer Soares Filho, Vivaldo Moura-Neto Frontiers in Neuroanatomy, 2020 Recent studies have suggested the neuroinvasive potential of severe acute respiratory coronavirus 2 (SARS-CoV-2). Notably, neuroinvasiveness might be involved in the pathophysiology of coronavirus disease 2019 (COVID-19). Some studies have demonstrated that synapse-connected routes may enable coronaviruses to access the central nervous system (CNS). However, evidence related to the presence of SARS-CoV-2 in the CNS, its direct impact on the CNS, and the contribution to symptoms suffered, remain sparse. Here, we review the current literature that indicates that SARS-CoV-2 can invade the nervous system. We also describe the neural circuits that are potentially affected by the virus and their possible role in the progress of COVID-19. In addition, we propose several strategies to understand, diagnose, and treat the neurological symptoms of COVID-19.
Evaluation of oral care protocols practice by dentists in Rio de Janeiro towards HIV/AIDS individuals Carina Maciel Silva-Boghossian, Brenda Azzariti Berrondo Boscardini, Claudia Maria Pereira, Edson Jorge Lima Moreira BMC Oral Health, 2020 Background The aim of this study was to evaluate the dentists’ knowledge and practice regarding HIV positive individuals’ oral care in Rio de Janeiro State. Methods Dentists from Rio de Janeiro State (n = 242) answered an electronic questionnaire on biosafety procedures, oral manifestations of AIDS, and knowledge of HIV infection. Collected information was stratified by gender, and data were analyzed using Chi-square and t tests. Results From the 14 oral manifestations investigated, oral candidiasis, necrotizing ulcerative gingivitis, and hairy leucoplakia were more associated with HIV, with no differences between the responses from men and women. Above 85% of the participants would be concerned about becoming infected with HIV after a needle/ sharp object injury and more than 80% of them were willing to be tested for HIV. However, significantly more women (98.8%), compared to men (91.3%), said they knew that HIV/ AIDS patients can contaminate dental care professionals, p = 0.007. There was a significant difference in the answers for the questions: “Are there special dental clinics for treatment of HIV/AIDS patients in Brazil?” (p = 0.044), and “Do the negative HIV tests surely indicate that the persons are free of viruses?” (p = 0.005). Significant differences between men and women were also observed regarding use of disposable mask (p = 0.01), and cap (p < 0.0001). Conclusion Most dentists who participated in the study presented a good knowledge on the care of HIV/ AIDS individuals, including biosafety protocols and in terms of the oral manifestations commonly associated to AIDS.
Glioblastoma Therapy in the Age of Molecular Medicine Luiz Henrique Medeiros Geraldo, Celina Garcia, Anna Carolina Carvalho da Fonseca, Luiz Gustavo Feijó Dubois, Tânia Cristina Leite de Sampaio e Spohr, Diana Matias, Eduardo Sabino de Camargo Magalhães, Rackele Ferreira do Amaral, Barbara Gomes da Rosa, Izabella Grimaldi, Felipe Sceanu Leser, José Marcos Janeiro, Lucy Macharia, Caroline Wanjiru, Claudia Maria Pereira, Vivaldo Moura-Neto, Catarina Freitas, Flavia Regina Souza Lima Trends in Cancer, 2019
Microglia/astrocytes–glioblastoma crosstalk: Crucial molecular mechanisms and microenvironmental factors Diana Matias, Joana Balça-Silva, Grazielle C. da Graça, Caroline M. Wanjiru, Lucy W. Macharia, Carla Pires Nascimento, Natalia R. Roque, Juliana M. Coelho-Aguiar, Cláudia M. Pereira, Marcos F. Dos Santos, Luciana S. Pessoa, Flavia R. S. Lima, Alberto Schanaider, Valéria P. Ferrer,, Vivaldo Moura-Neto Frontiers in Cellular Neuroscience, 2018
MiRNAs: Important Targets for Oral Cancer Pain Research Cláudia Maria Pereira, Dayany Sehnem, Estevão Oliveira da Fonseca, Heráclito Fernando Gurgel Barboza, Antônio Carlos Pires de Carvalho, Alexandre F. M. DaSilva, Vivaldo Moura-Neto, Marcos F. DosSantos Biomed Research International, 2017
Gliomas and the vascular fragility of the blood brain barrier Luiz Gustavo Dubois, Loraine Campanati, Cassia Righy, Isabella D’Andrea-Meira, Tania Cristina Leite de Sampaio e Spohr, Isabel Porto-Carreiro, Claudia Maria Pereira, Joana Balça-Silva, Suzana Assad Kahn, Marcos F. DosSantos, Marcela de Almeida Rabello Oliveira, Adriana Ximenes-da-Silva, Maria Celeste Lopes, Eduardo Faveret, Emerson Leandro Gasparetto, Vivaldo Moura-Neto Frontiers in Cellular Neuroscience, 2014
Methylation pattern of IFNG in periapical granulomas and radicular cysts Kelma Campos, Carolina Cavaliéri Gomes, Jeane de Fátima Correia-Silva, Lucyana Conceição Farias, Thiago Fonseca–Silva, Vanessa Fátima Bernardes, Cláudia Maria Pereira, Ricardo Santiago Gomez Journal of Endodontics, 2013
Glioblastomas and the special role of adhesion molecules in their invasion Vivaldo Moura-Neto, Loraine Campanati, Diana Matias, Claudia Maria Pereira, Catarina Freitas, Juliana M. Coelho-Aguiar, Tania Cristina Leite de Sampaio e Spohr, Ana Lucia Tavares-Gomes, Diego Pinheiro-Aguiar, Suzana Assad Kahn, Joana Silva-Balça, Bruno Pontes, Isabel Porto-Carreiro, Jane Faria, Rodrigo Alves Portela Martins, Silvia Lima-Costa, Maria de Fátima Dias-Costa, Maria Celeste Lopes, Flavia Regina Souza Lima Glioma Cell Biology, 2012
Claudin-7 down-regulation is an important feature in oral squamous cell carcinoma Silvia Vanessa Lourenço, Cláudia Malheiros Coutinho‐Camillo, Marcilei Elisa Cavicchioli Buim, Ana Carolina De Carvalho, Roberta Cardim Lessa, Cláudia Maria Pereira, André Luiz Vettore, André Lopes Carvalho, José Humberto Fregnani, Luiz Paulo Kowalski, Fernando Augusto Soares Histopathology, 2010
