Full Professor and Provost for Research, Graduate Studies, and Extension, Vila Velha University (UVV), Department of Plant Biotechnology. Vila Velha University
Alessandro Coutinho Ramos is a plant biotechnology scientist with over 20 years of experience in soil microbiology and plant–microorganism interactions. He is Full Professor and Provost for Research, Graduate Studies, and Extension at Vila Velha University (UVV), Brazil. His research focuses on the role of microorganisms in plant productivity and sustainable agriculture, including ecophysiology, bioenergy, and soil biochemistry.
He has extensive experience supervising undergraduate, master’s, Ph.D., and postdoctoral researchers and actively collaborates on national and international research projects. In addition to his academic work, he is engaged in innovation and entrepreneurship, mentoring startups and leading initiatives that connect science to real-world impact.
EDUCATION
Ph.D. in Plant Production, State University of Northern Rio de Janeiro (UENF), Brazil
M.Sc. in Plant Production, State University of Northern Rio de Janeiro (UENF), Brazil
B.Sc. in Agronomy, Federal University of Viçosa (UFV), Brazil
Postdoctoral Research, Gulbenkian Institute of Science (IGC), Portugal
RESEARCH, TEACHING, or OTHER INTERESTS
Plant Science, Agricultural and Biological Sciences, Soil Science, Agronomy and Crop Science
51
Scopus Publications
Scopus Publications
Genome-resolved characterization of microbial consortia driving glyphosate degradation in soil Mariana Prósperi de Oliveira Paula, Alessandro de Mello Varani, Vitor Luciano Costa da Silva, Luiz Fernando Wurdig Roesch, Marcos Rogério Tótola, Alessandro Coutinho Ramos, Victor Satler Pylro Chemosphere, 2026 Glyphosate is a widely used non-selective herbicide associated with ecological and human health concerns due to its environmental persistence, highlighting the need for effective remediation strategies. Among available approaches, microbial enzyme-mediated degradation represents a promising biological solution. This study aimed to enrich and characterize glyphosate-degrading microbial consortia from coffee plantation soils, validate glyphosate and aminomethylphosphonic acid (AMPA) degradation by chromatographic analyses, and integrate genome-based functional annotation with comparative structural analyses to investigate enzymatic systems involved in C-P and C-N bond cleavage. The enrichment process, followed by metataxonomic and metagenomic analyses, revealed dynamic shifts in microbial community composition. Achromobacter and Serratia were identified as key genera, harboring genetic potential for glyphosate and AMPA degradation. High-performance liquid chromatography with diode array detection confirmed efficient transformation of both compounds, with consortia Con_CC and Con_CC-G achieving the highest removal efficiencies under carbon- and phosphorus-limited conditions. Genome-based functional annotation showed that both genera encode gene clusters associated with the C-P lyase pathway, while only Achromobacter harbors the gene encoding glyphosate oxidoreductase (GOX), linked to oxidative C-N bond cleavage. Structural modeling indicated conservation of key catalytic residues in PhnJ, whereas GOX-related sequences in Serratia corresponded to partial homologs lacking a complete catalytic site. By integrating chromatographic, genomic, and structural analyses, this study provides a multi-level framework linking microbial community dynamics, functional potential, and molecular mechanisms underlying glyphosate degradation.
Silicon Sources Differentially Affect Physiological Responses, Nutrient Uptake, and Phenolic Compounds in Sour Passion Fruit Rozane Franci de Moraes Tavares, Almy Junior Cordeiro de Carvalho, Simone de Paiva Caetano Bucker Moraes, Henrique Martins de Oliveira, Álan Chrisleyr Maracahipes, Paulo Cesar dos Santos, Moises Zucoloto, Alessandro Coutinho Ramos, Weverton Pereira Rodrigues, Tâmara Moreira Silva, Marta Simone Mendonça Freitas, Gabriel Ramatis Pugliese Andrade, Vinicius de Freitas Manhães, Marlene Evangelista Vieira, José Luiz Leonardo de Araújo Pimenta Horticulturae, 2026 This study evaluated the effects of two silicon sources (silicic acid and Agrisil) and increasing Si concentrations on physiological responses, total polyphenol content, photochemical performance, nutrient uptake, and phenolic metabolism in sour passion fruit (Passiflora edulis Sims) grown under soilless culture conditions. The experiment was conducted in a greenhouse using increasing concentrations of Si applied through the nutrient solution. Gas exchange parameters, chlorophyll index (SPAD), chlorophyll fluorescence variables, leaf temperature, and the contents of Si, nitrogen, and total polyphenols in leaves and roots were evaluated. Moderate Si concentrations enhanced stomatal conductance and transpiration, improving intrinsic water use efficiency, and maintaining higher chlorophyll levels and photochemical performance. In contrast, higher Si concentrations increased Si deposition in leaf tissues, reduced stomatal regulation and transpiration, and increased leaf temperature. These changes were associated with reductions in chlorophyll index and photochemical performance index (PI), as well as increased F0/Fm. Net CO2 assimilation remained relatively stable. Silicon uptake differed between sources, with silicic acid showing faster absorption and Agrisil a more gradual release. Silicon fertilization also increased nitrogen uptake and stimulated the accumulation of phenolic compounds in roots. Overall, moderate silicon supplies enhanced physiological stability, whereas excessive accumulation imposed photochemical constraints.
Diazotrophic Bacteria and Nitrogen Fertilization on ATPase Activity in Micropropagated Pineapple Plantlets During Acclimatization Aurilena de Aviz Silva, Almy Junior Cordeiro de Carvalho, Paulo Cesar dos Santos, Rômulo André Beltrame, Marta Simone Mendonça Freitas, Flávia Paiva de Freitas, Roberto Rivelino do Nascimento Barbosa, Alessandro Coutinho Ramos, Fabio Lopes Olivares, Stella Arndt, Leandro Pin Dalvi, Moises Zucoloto, Orlando Carlos Huertas Tavares, Mírian Peixoto Soares da Silva Horticulturae, 2026 Micropropagated plantlets, after removal from controlled laboratory conditions, require an acclimatization period. Adaptation to the new environment induces anatomical and physiological changes controlled by cellular processes. This study investigated the involvement of the primary proton transport systems of total membranes in pineapple root colonization by diazotrophic bacteria and in the development of plantlets treated with different nitrogen doses, allowing an understanding of nutrient absorption and accumulation dynamics. The experiment followed a randomized block design (RBD) in a factorial scheme (2 × 3 × 2), with two inocula (a mixture of diazotrophic bacteria containing Burkholderia sp. UENF 114111, Burkholderia silvatlantica UENF 117111, and Herbaspirillum seropedicae HRC 54, and another without bacteria), three urea doses (0, 5, and 10 g L−1), and two evaluation (90 and 150 days) and bacterial counting times (30 and 150 days), with three blocks. Diazotrophic bacterial populations were lower in older plantlets. H+ transport mediated by P H+-ATPases changed with acclimatization time. Inoculation did not induce transport; however, the Fmax of V H+-ATPase was lower without nitrogen fertilization. Nitrogen fertilization affected V H+-ATPase proton transport activity in root membranes. The presence of diazotrophic bacteria did not induce proton transport. On the other hand, nitrogen fertilization and acclimatization time affected the proton transport activity mediated by H+-ATPases isolated from roots of micropropagated pineapple.
H+-ATPases Regulated by Auxin and ABA Mediate Acid Growth of Soybean Embryonic Axis During Germination Jacymara Lopes Pereira, Geovanna Vitória Olimpio, Fernanda Silva Coelho, Maria Luiza Carvalho Santos, Juliana Lopes Moraes, Deise Paes, Sara Sangi, Amanda Azevedo Bertolazi, Alessandro Coutinho Ramos, Clícia Grativol Seeds, 2025 Soybean seeds (Glycine max) are of great economic and nutritional importance due to their high oil and protein content. Seed germination is an essential process that influences crop yield and quality. The seed embryo resumes growth when it imbibes, which induces the expression of genes related to cell expansion. The role of acid growth in the embryonic axis during germination is not well characterized. Thus, the aim of this study was to verify the contribution of acid growth in the soybean embryonic axis germination. Acid growth is mediated by the acidification of extracellular medium due to the action of H+-ATPases, which activate expansins. We found that the expression of expansins was significantly increased throughout the germination. The expression of H+-ATPases was significantly increased at 3 and 24 h after imbibition (HAI), with major pumping activity at 24 HAI. The auxin and ABA signaling cascades during soybean germination suggest that these hormones are involved with the regulation of H+-ATPase in germinating soybean. To verify the influence of auxin and ABA on H+-ATPase functioning during germination, we treated seeds with IAA, 2,4D, ABA, and ATPase inhibitor, and germinated them in purple agar medium. We observed that IAA, 2,4D, and ABA affected H+-ATPase functioning, by delaying or inhibiting soybean germination. Our results indicate the role of acid growth controlled by H+-ATPase and its regulators—auxin and ABA—in the soybean embryonic axis during germination.
Isolation and Characterization of Glyphosate-Tolerant Bacteria from a Local Coffee Plantation in Espírito Santo, Brazil Bianca C. Silva, Brena G. de Souza, Amanda A. Bertolazi, Arthur R. Pêgos, Cristina Cruz, Juliana Melo, Olubukola O. Babalola, César A. Krohling, Alessandro C. Ramos ACS Agricultural Science and Technology, 2025 High Resolution Image Download MS PowerPoint Slide This study aimed to isolate glyphosate-tolerant bacteria and characterize their plant-beneficial traits. Using a bioprospecting and glyphosate enrichment methodology, we isolated 35 bacterial strains that were tested for tolerance to glyphosate on solid and liquid media (0, 11, 22, 44, and 88 g L –1 of glyphosate). Sixteen isolates with high tolerance to glyphosate (≥22 g L –1 ) were identified and characterized by their ability to fix atmospheric nitrogen, solubilize inorganic phosphate, and produce indole-3-acetic acid (IAA). After similarity analysis and compatibility testing, a consortium composed of three bacteria ( Priestia sp.; Achromobacter sp.; Pseudomonas sp.) were selected to be applied in a model plant (tomato) and Arabica coffee beans. The bacterial consortium promoted the germination (96.6%) and development of tomato seedlings, while also degrading residual glyphosate (31.25%) in coffee beans, as evidenced by the reduced levels of the herbicide. These results demonstrate the bacterial consortium’s potential to reduce glyphosate residues and sustainably increase plant productivity.
Air Quality in Low-Income Households of Asthmatic Children Érica Coelho Pagel, Lidia Leal Medeiros, Jane Méri Santos, Clarisse Maximo Arpini, Alessandro Coutinho Ramos, Neyval Costa Reis Júnior Revista Invi, 2025 Residências construídas sem a orientação profissional adequada, conhecidas como autoconstruções, muitas vezes apresentam inconformidades que podem prejudicar a qualidade do ar interno, contribuindo para o aumento da concentração de contaminantes microbiológicos no ar. O presente trabalho objetivou identificar a relação entre os aspectos construtivos da moradia e a qualidade do ar em um conjunto de residências de crianças asmáticas na cidade de Vitória, Espírito Santo, Brasil. A metodologia incluiu levantamento físicos e fotográficos, identificação visual da presença de mofo nas residências e coleta de fungos aéreos. Os resultados mostraram existência de alta umidade nas moradias e janelas nos dormitórios com área de ventilação e iluminação natural abaixo do mínimo recomendado pela legislação brasileira. A concentração de fungos no ar ultrapassou o limite da OMS em 29 das 56 moradias analisadas. O estudo pretende colaborar no entendimento dos aspectos entre a habitação e a saúde humana reduzindo assim o agravamento de problemas respiratórios e melhorando a qualidade de vida.
How Can Plant-microbe Interactions be used for the Bioremediation of Metals in Water Bodies? Gabriela Petroceli‐Mota, Emilane Pinheiro da Cruz Lima, Mariana Miranda de Abreu, Glacielen Ribeiro de Souza, Jussara Tamires de Souza Silva, Gabriel Quintanilha‐Peixoto, Alessandro Coutinho Ramos, Rachel Ann Hauser‐Davis, Aline Chaves Intorne Microbes Based Approaches for the Management of Hazardous Contaminants, 2024 Water quality declines are a serious issue in todays world due to a lack of sanitation and adequate wastewater treatment, leading to significant aquatic contamination. In this context, metals comprise one of the most worrying classes of contaminants, due to their non-biodegradability and bioavailability, contaminating water sources intended for human consumption. Therefore, treatment methods capable of mitigating environmental metal contamination are paramount. Bioremediation mediated by plant-microbe associations is a promising treatment technique in this regard, due to low costs and sustainability. This chapter addresses water distribution and treatment issues and metal aquatic ecosystem contamination, emphasizing the use of aquatic plants and microorganisms able to promote plant growth displaying wastewater remediation potential.
Microorganisms as Biocontrol Agents of Pests and Diseases Muriel da Silva Folli-Pereira, Amanda Azevedo Bertolazi, Juliana Garlet, Lucas Zanchetta Passamani, Sávio Bastos de Souza, Fernanda Senna Ferreira Costa, Alessandro Coutinho Ramos Microorganisms for Sustainability, 2022
Plasma membrane H+pump at a crossroads of acidic and iron stresses in yeast-to-hypha transition Antônio Jesus Dorighetto Cogo, Arnoldo Rocha Façanha, Layz Ribeiro da Silva Teixeira, Sávio Bastos de Souza, Janiélio Gonçalves da Rocha, Frederico Firme Figueira, Frederico Jacob Eutrópio, Amanda Azevedo Bertolazi, Carlos Eduardo de Rezende, Cesar Abel Krohling, Lev A Okorokov, Cristina Cruz, Alessandro Coutinho Ramos, Anna L Okorokova-Façanha Metallomics, 2020
Soil: Do not disturb, mycorrhiza in action Cristina Cruz, Alessandro Ramos, Olubukola Oluranti Babalola, Hessini Kamel, Teresa Dias, Ajit Varma Mycorrhiza Function Diversity State of the Art Fourth Edition, 2017
Ecophysiology of iron homeostasis in plants Cesar Abel Krohling, Frederico Jacob Eutrópio, Amanda Azevedo Bertolazi, Leonardo Barros Dobbss, Eliemar Campostrini, Teresa Dias, Alessandro Coutinho Ramos Soil Science and Plant Nutrition, 2016
Toxic levels of iron in conilon coffee crops (Coffea canephora) in soils from the coastal tablelands Coffee Science, 2016
Water stress and higher plants: An overview Muriel da Silva Folli‐Pereira, Alessandro Coutinho Ramos, Amanda Azevedo Bertolazi, Lucas Zanchetta Passamani, Frederico Jacob Eutrópio, Juliana Melo da Conceição, Nazima Rasool Water Stress and Crop Plants A Sustainable Approach, 2016
Foliar application of trace elements in alleviating drought stress Muriel da Silva Folli‐Pereira, Alessandro Coutinho Ramos, Gabriela Chaves Canton, Juliana Melo da Conceição, Sávio Bastos de Souza, Antônio Jesus Dorighetto Cogo, Frederico Firme Figueira, Frederico Jacob Eutrópio, Nazima Rasool Water Stress and Crop Plants A Sustainable Approach, 2016
Heavy Metal Stress and Molecular Approaches in Plants Frederico Jacob Eutrópio, Alessandro Coutinho Ramos, Muriel da Silva Folli-Pereira, Natália de Aquino Portela, Janaina Biral dos Santos, Juliana Melo da Conceição, Amanda Azevedo Bertolazi, Frederico Figueira Firme, Sávio Bastos de Souza, Antônio Jesus Dorighetto Cogo, Nazima Rasool Plant Metal Interaction Emerging Remediation Techniques, 2015
An outlook on ion signaling and ionome of mycorrhizal symbiosis Alessandro C. Ramos, Arnoldo R. Façanha, Livia M. Palma, Lev A. Okorokov, Zilma M.A. Cruz, Ary G. Silva, Arthur F. Siqueira, Amanda A. Bertolazi, Gabriela C. Canton, Juliana Melo, Wolmen O. Santos, Vanusa M. B. Schimitberger, Anna L. Okorokova-Façanha Brazilian Journal of Plant Physiology, 2011
Author and co-author of 50+ peer-reviewed publications in international journals, including PLANTA, Chemosphere, Frontiers in Microbiology, and Environmental Science and Pollution Research. Research impact includes hundreds of citations and contributions to plant–microbe interactions, soil microbiology, and sustainable agriculture.
GRANT DETAILS
Participation and leadership in funded research projects supported by agencies such as CNPq, CAPES, FAPES, and FAPERJ. Experience in managing research funding, innovation grants, and multi-institutional projects.
RESEARCH OUTPUTS (PATENTS, SOFTWARE, PUBLICATIONS, PRODUCTS)
Development of bioinoculants and microbial-based solutions for sustainable agriculture. Contributions to applied research in bioremediation, plant growth promotion, and agricultural biotechnology. Participation in innovation projects focused on translating scientific knowledge into practical applications.
CONSULTANCY
Consultant and mentor in innovation, entrepreneurship, and research project development. Advisor to startups and organizations in areas related to biotechnology, agriculture, and innovation ecosystems.
Industry, Institute, or Organisation Collaboration
Active collaborations with universities and research centers in Brazil and abroad, including UENF, University of Lisbon, and international partners. Engagement with innovation agencies such as FAPES, FINEP, and Sebrae, supporting research, entrepreneurship, and technology transfer initiatives.
INDUSTRY EXPERIENCE
Experience in innovation program leadership, startup acceleration, and technology transfer. Advisor and evaluator for startups in national programs such as Centelha, Catalisa ICT, and SEEDES (Government of Espírito Santo).
STARTUP
Co-founder of Symbiotec, a biotechnology startup focused on microbial solutions for agriculture, selected in the national Catalisa ICT program (Sebrae). Active mentor and evaluator of startups within Brazilian innovation programs.
SOCIAL, ECONOMIC, or ACADEMIC BENEFITS
Research contributes to sustainable agriculture, environmental recovery, and increased productivity through plant–microorganism interactions. Strong impact on human resource training, innovation ecosystems, and science-based entrepreneurship at regional and national levels.