PhD in Food Technology from the University of Campinas (Unicamp) (2005). Currently is Associate Professor of the Faculty of Food Engineering (FEA) of UFPA and is a advisor on the Graduate Program in Food Science and Technology of UFPA (PPGCTA). Experience in the field of Food Science and Technology. Develops research and extension activities in the fields of Food Chemistry, Biochemistry and Biotechnology, Vegetable Raw Materials, Pigment Extraction and Bioactive Compounds.
Cyanogenic Compounds, Physicochemical Parameters and Metals in Water From Cassava Processing Areas: A Study in the Brazilian Amazon, Using Multivariate Analysis Alana Coêlho Maciel, Mariane Gama Nabiça, Luciana Pinheiro Santos, Thiago de Melo e Silva, Elivaldo Nunes Modesto Junior, Heronides Adonias Dantas Filho, Charles Miller de Souza Borges, Kelly das Graças Fernandes Dantas, Simone de Fátima Pinheiro Pereira, Ivan Carlos da Costa Barbosa, Beni Jequicene Mussengue Chaúque, Rosinelson da Silva Pena, Nayara Macêdo Peixoto Araújo, Alessandra Santos Lopes Environmental Toxicology, 2026 This study evaluated the water quality of artesian wells and effluents generated in cassava processing in a rural area of Bragança, Pará, with emphasis on physicochemical parameters, multielement composition, and cyanogenic compounds. The well water presented specific nonconformities, highlighting pH values below the recommended level and high concentrations of residual chlorine. Although cyanide was not detected in the groundwater, the effluents presented concentrations of total and free cyanide above legal limits, mainly in the pressing water and in the washing and fermentation tanks, indicating an environmental risk associated with the inadequate disposal of these residues. Regarding the multielement analysis, boron and copper enrichment was observed in the well water, and high concentrations of manganese and nickel were found in the effluents, exceeding the limits established by Brazilian environmental legislation. The multivariate analysis revealed distinct physicochemical profiles between the well water and the effluents, with temperature, dissolved oxygen, and pH being the main factors responsible for the differentiation between the matrices. The results indicate the need for improvements in the management and treatment systems of cassava processing effluents, aiming at protecting water resources and reducing potential environmental and human health risks.
Açai Seeds, a Highly Discarded Byproduct in the Amazonia, as Potential Alternative Substrate for the Biotechnological Production of Carotenoids Willen Silva Igreja, Felipe de Andrade Maia, Gilson Celso Albuquerque Chagas Junior, Alessandra Santos Lopes, Renan Campos Chisté ACS Agricultural Science and Technology, 2025 Rhodotorula yeasts can biosynthesize carotenoids inside their cells when they are subjected to adverse environmental conditions, and this biotechnological process can be industrially viable if the production cost can be minimized using low-cost byproducts as substrates. This research investigated the production of carotenoids using açaí seeds as a substrate for R. mucilaginosa. The best conditions for the production of biomass of R. mucilaginosa were pH 7.0, 40 °C, and agitation at 200 rpm, while those for the biosynthesis of carotenoids were pH 7.0, 30 °C, and 200 rpm. The optimized conditions provided 2 g of dry biomass and total carotenoid contents of 37 μg/g of dry biomass, with β-carotene (77%), Z-γ-carotene (6.9%), and torulene (4.5%) being the major carotenoids. Therefore, the biotechnological production of carotenoids achieved by R. mucilaginosa using açai seeds deserves scientific attention as a promising alternative to the use of waste processing units as a sustainable strategy for a circular economy.
Multiomics analysis reveals microbial diversity and activity through spontaneous fermentation of Theobroma cacao Ynara da Costa Fonseca, Celina Eugenio Bahule, Hector Herrera, Luiza Helena da Silva Martins, Alessandra Santos Lopes, Juliana Silva Cassoli, Felipe Costa Trindade, Isa Rebecca Chagas da Costa, Paulo Henrique de Oliveira Costa, Guilherme Oliveira, Rafael Borges da Silva Valadares Heliyon, 2024 <h2>Abstract</h2> To gain insight into the active microbiota during spontaneous fermentation of <i>Theobroma cacao</i> L., this study assessed protein diversity during 120 h using a combined metabarconding and metaproteomics approach. During the first days of fermentation, most of the peptides were associated with <i>T. cacao</i> and yeast (0–72 h). Peptides associated with bacteria became more abundant after 72 h of fermentation, coinciding with a decrease in peptides associated with cocoa (96–120 h). In addition to the known microorganisms involved in fermentation, such as <i>Saccharomyces</i>, <i>Lactobacillus</i> and <i>Acetobacter</i>, novel genera were also metabolically active, including <i>Microvirga</i>, <i>Inquilinus</i>, <i>Candolleomyces</i> and <i>Lasiodiplodia</i>.. The results showed a consistency in the main genera detected by both techniques, but the identification of unexplored genera such as <i>Inquilinus</i>, <i>Microvirga</i>, <i>Cyphellophora</i> and <i>Ashbya gossypii</i>, among others, suggests that this omics approach needs to be used together for more comprehensive results on spontaneous fermentation. In conclusion, studies combining techniques such as metabarcoding and metaproteomics should be considered in fermentation studies, as a single technique would result in omissions regarding the activity of certain microorganisms that may be important for the course of spontaneous fermentation.
Cassava Starch/Carboxymethyl Cellulose Edible Coating Added of Tocopherol: A Strategy to Preserve the Oxidative Stability of Brazil Nuts Danusa Silva da Costa, Kalebe Ferreira Furtado, Ariane Mendonça Kluczkovski, Katiuchia Pereira Takeuchi, Alessandra Santos Lopes Foods, 2024 The aim was to apply a cassava starch/carboxymethyl cellulose blend-based edible coating added to a tocopherol mix to Brazil nuts and evaluate oxidative levels during storage. The edible coatings were prepared from a cassava starch/carboxymethyl cellulose blend and identified as control B (no soy lecithin and no tocopherol mix), L (with soy lecithin and no tocopherol mix), and LT and LT2 (with soy lecithin and tocopherol mix). In the forming solutions of the coatings, stability, viscosity, pH, and color were analyzed. The Brazil nuts were immersed in the solutions for 30 s, dried at 45 °C, and placed in an incubator at 25 °C. At 1, 7, 15, 30, 45, 60, 90, and 120 days of storage, mass loss, the browning index, conjugated dienes and trienes, the oxidative state by official methods, and the accelerated oxidation index were evaluated. The blend-forming solutions B, L, LT, and LT2 showed non-Newtonian and pseudoplastic behavior, excellent resistance to flow, and stability. The diene, triene, iodine value, peroxide value, p-anisidine value, and total oxidation indices showed that the application of the cassava starch/carboxymethyl cellulose blend-based edible coating added tocopherol mix, LT, and LT2 preserved the Brazil nuts up to 90 days of storage at 25 °C. PCA shows that all coatings applied to Brazil nuts promoted oil preservation in some evaluation periods, especially those added with a tocopherol mix. It is concluded that cassava starch/CMC added tocopherol mix edible coatings have a potential application as active packaging for foods, especially nuts.
Amino acid profile behavior during the fermentation of Criollo cocoa beans César R. Balcázar-Zumaeta, Editha Fernández-Romero, Alessandra Santos Lopes, Nelson Rosa Ferreira, Gilson Celso Albuquerque Chagas-Júnior, Ives Yoplac, Heydi A. López-Trigoso, Mery L. Tuesta-Occ, Italo Maldonado-Ramirez, Jorge L. Maicelo-Quintana, Ilse S. Cayo-Colca, Efrain M. Castro-Alayo Food Chemistry X, 2024 The study investigated the behavior of seventeen amino acids during spontaneous (SF) and starter culture (SC) fermentation of Criollo cocoa beans from Copallín, Guadalupe and Tolopampa, Amazonas-Peru. For this purpose, liquid chromatography (UHPLC) was used to quantify amino acids. Multivariate analysis was used to differentiate the phases of the fermentation process. The percentage of essential amino acids during SC fermentation (63.4%) was higher than SF (61.8%); it was observed that the starter culture accelerated their presence and increased their concentration during the fermentation process. The multivariate analysis identified a first stage (day 0 to day 2), characterized by a low content of amino acids that increased due to protein hydrolysis. The study showed that adding the starter culture (Saccharomyces cerevisiae) to the fermentation mass increased the concentration of essential amino acids (63.0%) compared to the spontaneous process (61.8%). Moreover, this addition reduced the fermentation time (3–4 days less), demonstrating that the fermentation process with a starter culture allows obtaining a better profile of amino acids precursors of flavor and aroma.
Properties of films and coatings added of tocopherol for food packaging: tool-based review for systematic reviews and bibliometric analysis Danusa Silva da Costa, Lucely Nogueira dos Santos, Nelson Rosa Ferreira, Katiuchia Pereira Takeuchi, Alessandra Santos Lopes British Food Journal, 2024 PurposeThe aim was not to perform a systematic review but firstly to search in PubMed, Science Direct, Scopus and Web of Science databases on the papers published in the last five years using tools for reviewing the statement of preferred information item for systematic reviews without focusing on a randomized analysis and secondly to perform a bibliometric analysis on the properties of films and coatings added of tocopherol for food packaging.Design/methodology/approachOn January 24, 2022, information was sought on the properties of films and coatings added of tocopherol for use as food packaging published in PubMed, Science Direct, Scopus and Web of Science databases. Further analysis was performed using bibliometric indicators with the VOSviewer tool.FindingsThe searches returned 33 studies concerning the properties of films and coatings added of tocopherol for food packaging, which were analyzed together for a better understanding of the results. Data analysis using the VOSviewer tool allowed a better visualization and exploration of these words and the development of maps that showed the main links between the publications.Originality/valueIn the area of food science and technology, the development of polymers capable of promoting the extension of the shelf life of food products is sought, so the knowledge of the properties is vital for this research area since combining a biodegradable polymeric material with a natural antioxidant active is of great interest for modern society since they associate environmental preservation with food preservation.
Technological parameters of cassava starch/carboxymethyl cellulose blend-based films added of soy lecithin and tocopherol mix Danusa Silva da Costa, Roseane Maria Ribeiro Costa, Katiuchia Pereira Takeuchi, Alessandra Santos Lopes Polymer Testing, 2023 The aim was to evaluate the technological properties of films based on cassava starch/carboxymethyl cellulose added to soy lecithin and tocopherol mix. The films were prepared by casting from a cassava starch/carboxymethyl cellulose mixture and identified as control B (no soy lecithin and no tocopherol mix), L (with soy lecithin and no tocopherol mix) and LT and LT2 (with soy lecithin and tocopherol mix). Thickness, water solubility, water content, film weight, contact angle, permeability test, mechanical tests, antioxidant activity, biodegradability, color, thermogravimetry, and FTIR tests were performed. The results showed that the films containing soy lecithin (L, LT, and LT2) were more hydrophilic and had a denser structure than film B, but the LT and LT2 films were slightly yellow-green, malleable and showed few bubbles or cracks. In addition, all the films developed are biodegradable. It was also found that the antioxidant remained in the films to which it was added (LT and LT2) and that the LT2 film showed the highest antioxidant activity. It is strongly recommended for application in active food packaging.
Cladosporium cladosporioides starter culture can positively influence flavour and bioactive compounds of fermented and dried cocoa beans from the Amazon biome Journal of Food and Nutrition Research, 2022
Study of the antioxidant power of the waste oil from palm oil bleaching clay Katiane Cunha de Melo, Ingrid Silva de Oliveira, Luiza Helena de Oliveira Pires, Luís Adriano Santos do Nascimento, José Roberto Zamian, Geraldo Narciso da Rocha Filho, Marcele Fonseca Passos, Alessandra Santos Lopes, Attilio Converti, Carlos Emmerson Ferreira da Costa Energies, 2020
Application of mycogenic nanoparticles against neurodegenerative diseases Jhonatas Rodrigues Barbosa, Maurício Madson dos Santos Freitas, Luiza Helena da Silva Martins, Andrea Komesu, Alessandra Santos Lopes, Raul Nunes de Carvalho Junior, Mahendra Rai Nanobiotechnology in Neurodegenerative Diseases, 2019
Sorption isotherms of tapioca flour Renan C. Chisté, Priscila A. Silva, Alessandra S. Lopes, Rosinelson da Silva Pena International Journal of Food Science and Technology, 2012
Pitanga (Eugenia uniflora L.) M. Vizzotto, L. Cabral, A. Santos Postharvest Biology and Technology of Tropical and Subtropical Fruits, 2011
Metagenomic and biogenic amine changes in cassava fermentation for tucupi production using Pediococcus acidilactici starter culture DG Brício, MBA Gloria, JAP Bitencourt, LA Argôlo, R de Lima Ribeiro, ... Journal of the Science of Food and Agriculture 106 (4), 2424-2436 , 2026 2026
Cyanogenic Compounds, Physicochemical Parameters and Metals in Water From Cassava Processing Areas: A Study in the Brazilian Amazon, Using Multivariate Analysis AC Maciel, MG Nabiça, LP Santos, T de Melo e Silva, ENM Junior, ... Environmental Toxicology , 2026 2026
Açai Seeds, a Highly Discarded Byproduct in the Amazonia, as Potential Alternative Substrate for the Biotechnological Production of Carotenoids WS Igreja, FA Maia, GCA Chagas Junior, AS Lopes, RC Chisté ACS Agricultural Science & Technology 5 (10), 2040-2049 , 2025 2025
Análise química, físico-química e citotoxicidade da Hibiscus sabdariffa L. AJ da Silva Costa, LSN dos Santos, DSB Brasil, JP Mares, GQ Moura, ... Observatorio de la Economía Latinoamericana 23 (7), e10515-e10515 , 2025 2025
Metaproteomic analysis of microbial diversity and activity in cassava fermentation for tucupi production AP de Sousa Cavalcante, CDGB Feio, NR Ferreira, GCA Chagas-Junior, ... Food Bioscience 68, 106423 , 2025 2025 Citations: 3
Metaproteomics revealing cyanide bioremediators in effluents from artisanal cassava processing in the Brazilian Amazon AC Maciel, SM Raad Nahon, IRC da Costa, RB Silva Valadares, ... bioRxiv, 2025.05. 20.655124 , 2025 2025
Néctar misto de cajá e umbu: avaliação sensorial e funcional. R MATTIETTO, AS LOPES, HC MENEZES 2025
Properties of films and coatings added of tocopherol for food packaging: tool-based review for systematic reviews and bibliometric analysis DS da Costa, LN dos Santos, NR Ferreira, KP Takeuchi, AS Lopes British Food Journal 126 (13), 226-268 , 2024 2024 Citations: 9
Multiomics analysis reveals microbial diversity and activity through spontaneous fermentation of Theobroma cacao Y da Costa Fonseca, CE Bahule, H Herrera, LH da Silva Martins, ... Heliyon 10 (23) , 2024 2024 Citations: 2
Cassava Starch/Carboxymethyl Cellulose Edible Coating Added of Tocopherol: A Strategy to Preserve the Oxidative Stability of Brazil Nuts DS da Costa, KF Furtado, AM Kluczkovski, KP Takeuchi, AS Lopes Foods 13 (17), 2732 , 2024 2024 Citations: 3
Amino acid profile behavior during the fermentation of Criollo cocoa beans CR Balcázar-Zumaeta, E Fernández-Romero, AS Lopes, NR Ferreira, ... Food Chemistry: X 22, 101486 , 2024 2024 Citations: 21
Metaproteomics revealing microbial diversity and activity in the spontaneous fermentation of maize dough CE Bahule, LH da Silva Martins, BJM Chaúque, F Trindade, H Herrera, ... Food Chemistry 435, 137457 , 2024 2024 Citations: 13
Revestimento comestível incorporado com tocoferóis e aplicação em Castanhado-Brasil (bertholletia excelsa bonpl.) para monitoramento do perfil de oxidação lipídica DS COSTA, AS LOPES, KP TAKEUCHI 2024
Total and Free Hydrogen Cyanide Content and Profile of Bioactive Amines in Commercial Tucupi , a Traditionally Derived Cassava Product Widely Consumed in … BNC Brito, MG Martins, RC Chisté, AS Lopes, MBA Gloria, RS Pena Foods 12 (23), 4333 , 2023 2023 Citations: 8
Technological parameters of cassava starch/carboxymethyl cellulose blend-based films added of soy lecithin and tocopherol mix DS da Costa, RMR Costa, KP Takeuchi, AS Lopes Polymer Testing 129, 108245 , 2023 2023 Citations: 13
Bioactive Compounds of Jambu ( Acmella oleracea (L.) R. K. Jansen) as Potential Components of Biodegradable Food Packing: A Review JS Moura, EM Gemaque, CE Bahule, LHS Martins, RC Chisté, AS Lopes Sustainability 15 (21), 15231 , 2023 2023 Citations: 9
Health exposure risks and bioremediation of cyanide in cassava processing effluents: An overview AC Maciel, R da Silva Pena, LD do Nascimento, TA de Oliveira, ... Journal of Water Process Engineering 55, 104079 , 2023 2023 Citations: 27
Açai Seeds ( Euterpe oleracea Mart) Are Agroindustrial Waste with High Potential to Produce Low-Cost Substrates after Acid Hydrolysis WS Igreja, LH da Silva Martins, RR de Almeida, JAR de Oliveira, ... Molecules 28 (18), 6661 , 2023 2023 Citations: 16
Mairá-Potato ( Casimirella sp.): Botanical, Food, Pharmacological, and Phytochemical Aspects DS Costa, LN Santos, NR Ferreira, KP Takeuchi, AS Lopes Molecules 28 (16), 6069 , 2023 2023 Citations: 2
Concentrated Manipueira as an Alternative Low-Cost Substrate to Rhodotorula glutinis for Biotechnological Production of High Contents of Carotenoids FA Maia, WS Igreja, AAO Xavier, AZ Mercadante, AS Lopes, RC Chisté Fermentation 9 (7), 617 , 2023 2023 Citations: 4
MOST CITED SCHOLAR PUBLICATIONS
Bioactive amines and phenolic compounds in cocoa beans are affected by fermentation BN do Carmo Brito, RC Chisté, R da Silva Pena, MBA Gloria, AS Lopes Food Chemistry 228, 484-490 , 2017 2017 Citations: 139
Biotechnological production of carotenoids using low cost-substrates is influenced by cultivation parameters: A review WS Igreja, FA Maia, AS Lopes, RC Chisté International Journal of Molecular Sciences 22 (16), 8819 , 2021 2021 Citations: 113
Chemical implications and time reduction of on-farm cocoa fermentation by Saccharomyces cerevisiae and Pichia kudriavzevii GCAC Junior, NR Ferreira, MBA Gloria, LH da Silva Martins, AS Lopes Food Chemistry 338, 127834 , 2021 2021 Citations: 71
Obtenção de barra de cereais adicionada do resíduo industrial de maracujá IQ da SILVA, BCF de OLIVEIRA, AS Lopes, P Rosinelson da Silva 2009 Citations: 69
Caracterização física e físico-química dos frutos da cajazeira (Spondias mombin L.) e de suas polpas obtidas por dois tipos de extrator. RA Mattietto, AS Lopes, HC De Menezes 2010 Citations: 68
Anthocyanins of Jambolão (S yzygium cumini ): Extraction and pH‐Dependent Color Changes BN do Carmo Brito, R da Silva Pena, A Santos Lopes, R Campos Chisté Journal of food science 82 (10), 2286-2290 , 2017 2017 Citations: 61
Determination of theobromine and caffeine in fermented and unfermented Amazonian cocoa (Theobroma cacao L.) beans using square wave voltammetry after chromatographic separation PCG Júnior, VB dos Santos, AS Lopes, JPI de Souza, JRS Pina, ... Food Control 108, 106887 , 2020 2020 Citations: 59
Estabilidade do néctar misto de cajá e umbu RA Mattietto, AS Lopes, HC Menezes Food Science and Technology 27 (3), 456-463 , 2007 2007 Citations: 56
An environment-friendly spot test method with digital imaging for the micro-titration of citric fruits LMA de Oliveira, VB Dos Santos, EKN da Silva, AS Lopes, ... Talanta 206, 120219 , 2020 2020 Citations: 53
Peach palm flour ( Bactris gasipae KUNTH): potential application in the food industry MB Pires, ER Amante, AS Lopes, AMC RODRIGUES, LHM SILVA Food Science and Technology 39, 613-619 , 2019 2019 Citations: 53
The microbiota diversity identified during the cocoa fermentation and the benefits of the starter cultures use: an overview GCA Chagas Junior, NR Ferreira, AS Lopes International Journal of Food Science and Technology 56 (2), 544-552 , 2021 2021 Citations: 52
Chemical composition, antioxidant activity, neuroprotective and anti-inflammatory effects of cipó-pucá (Cissus sicyoides L.) extracts obtained from supercritical extraction MAR Salazar, JV Costa, GRO Urbina, VMB Cunha, MP Silva, ... The Journal of Supercritical Fluids 138, 36-45 , 2018 2018 Citations: 46
Determination of process parameters and bioactive properties of the murici pulp (Byrsonima crassifolia) extracts obtained by supercritical extraction FCS Pires, APS e Silva, MAR Salazar, WA da Costa, HSC da Costa, ... The Journal of Supercritical Fluids 146, 128-135 , 2019 2019 Citations: 37
Profile of volatile compounds of on-farm fermented and dried cocoa beans inoculated with Saccharomyces cerevisiae KY794742 and Pichia kudriavzevii KY794725 GCA Chagas Junior, NR Ferreira, EHA Andrade, LD Nascimento, ... Molecules 26 (2), 344 , 2021 2021 Citations: 36
Amazonian Eryngium foetidum leaves exhibited very high contents of bioactive compounds and high singlet oxygen quenching capacity DSTC Leitão, FC Siqueira, SHB de Sousa, AZ Mercadante, RC Chiste, ... International Journal of Food Properties 23 (1), 1452-1464 , 2020 2020 Citations: 29
Filamentous fungi diversity in the natural fermentation of Amazonian cocoa beans and the microbial enzyme activities JA de Araújo, NR Ferreira, SHM da Silva, G Oliveira, RC Monteiro, ... Annals of Microbiology 69 (9), 975-987 , 2019 2019 Citations: 28
Qualidade nutricional das proteínas de cupuaçu e de cacau AS Lopes, NH Pezoa-García, J Amaya-Farfán Food Science and Technology 28, 263-268 , 2008 2008 Citations: 28
Health exposure risks and bioremediation of cyanide in cassava processing effluents: An overview AC Maciel, R da Silva Pena, LD do Nascimento, TA de Oliveira, ... Journal of Water Process Engineering 55, 104079 , 2023 2023 Citations: 27
Caracterização de farinhas de tapioca produzidas no estado do Pará PA Silva, RL Cunha, AS Lopes, RS Pena Ciência Rural 43 (1), 185-191 , 2012 2012 Citations: 27
Diversity of yeasts during fermentation of cocoa from two sites in the Brazilian Amazon SFO ALMEIDA, LRC Silva, GCAC JUNIOR, G Oliveira, SHM Silva, ... Acta Amazonica 49 (1), 64-70 , 2019 2019 Citations: 25