Somayeh rajabi
@iau-shahrood.ac.ir
Scopus Publications
- Effect of Four Weeks of Aerobic Exercise and Berberine Supplementation on the Expression of Dopamine 5 Receptor and Poly (Adenosin Diphosphat [ADP]-Ribose) Polymerase Genes in the Heart Tissue of Methamphetamine-Exposed Rats
Zahra Aghajani, Somayeh Rajabi, Sayyed-Javad Ziaolhagh
Journal of Gorgan University of Medical Sciences, 2024
Background and Objective: Methamphetamine is a highly addictive drug associated with severe psychosocial consequences and is extensively abused. This study aimed to determine the effects of four weeks of aerobic exercise and berberine supplementation on the expression of dopamine 5 receptor and poly (adenosin diphosphat [ADP]-ribose polymerase (PARP) genes in the heart tissue of methamphetamine-exposed rats. Methods: In this experimental study, 30 female Wistar rats were randomly allocated into five groups of six: Control, methamphetamine, methamphetamine + aerobic exercise, methamphetamine + berberine, and methamphetamine + aerobic exercise + berberine. Intraperitoneal injections of methamphetamine (10 mg/kg) and aerobic exercise and berberine consumption (100 mg/kg) were administered for 4 weeks during the withdrawal period. The expression levels of dopamine 5 receptor and PARP genes was measured using real-time polymerase chain reaction (PCR). Results: There was no significant difference in PARP gene expression between the methamphetamine group (1.02±0.65) and the control group (1.02±0.24). Similarly, there was no significant difference in dopamine 5 receptor gene expression between the methamphetamine group (5.74±4.94) and the control group (4.76±2.63). The expression levels of PARP and dopamine 5 receptor genes following exercise (1.01±0.55 and 4.30±1.96, respectively), berberine supplementation (0.61±0.25 and 2.97±1.45, respectively), and the combined intervention (0.67±0.30 and 3.43±1.87, respectively) showed no significant differences between the groups. Conclusion: Short-term methamphetamine induction did not induce significant changes in the expression of dopamine 5 receptor and PARP genes in the hearts of methamphetamine-exposed rats. - Examining the Cardioprotective Effects of Aerobic Exercise Combined with Crocin or Berberine on Methamphetamine-Induced Heart Damage in Female Rats
Sayed Javad Ziaolhagh, Somayeh Rajabi, Zahra Nosrati
International Journal of High Risk Behaviors and Addiction, 2024
Background: While the harmful effects of methamphetamine abuse on the heart are well-known, the solutions to deal with these damages are not well-known. Objectives: This study aimed to investigate whether aerobic exercise combined with supplements such as Crocin and Berberine can potentially protect against methamphetamine-induced changes in the heart tissue of female rats. Materials and Methods: Forty-two female rats were randomly assigned into seven groups (n = 6). The groups included healthy control, methamphetamine, methamphetamine + exercise, methamphetamine + Crocin, methamphetamine + Berberine, methamphetamine + exercise + Crocin, and methamphetamine + exercise + Berberine group. Methamphetamine (10 mg/kg) was injected intraperitoneally over five days. The animals were administered Crocin via intraperitoneal injection at 40 mg/kg during a four-week withdrawal period. At the same time, Berberine was dissolved in drinking water at 100 mg/kg concentration. The aerobic training consisted of running at 25 m/min for 30 minutes. A light microscope was used to observe the structural changes. Data analysis was performed using non-parametric Kruskal-Wallis and Mann-Whitney U tests by SPSS 26, with a significance level of 5%. Results: The results of the study indicate that methamphetamine use causes hyperemia, inflammation, and histopathological damage in the heart cells and tissue (P = 0.001). The combination of exercise with Crocin significantly eliminated methamphetamine-induced heart tissue damage (P = 0.001) and improved cardio-respiratory endurance compared to control (P = 0.004) and methamphetamine (P = 0.01) groups. The combination of aerobic exercise with Berberine removed methamphetamine-induced heart cell damage (P = 0.001), and improved cardio-respiratory endurance compared to control (P = 0.024) and methamphetamine (P = 0.05) groups. In addition, all intervention groups showed a significant reduction in hyperemia and inflammation compared to the methamphetamine group (P = 0.001). Conclusions: A combination of exercise with either Crocin or Berberine supplements demonstrated potential benefits in mitigating some of the harmful structural effects of methamphetamine and improving cardio-respiratory endurance. - The Effect of Psilocybin and Aerobic Exercise on the Gene Expression of PARP and Dopamine 5 Receptor in Heart Tissue of Female Rats Addicted to Methamphetamine
فاطمه یاورپناه, سمیه رجبی, سید جواد ضیاءالحق
Journal of Isfahan Medical School, 2024
مقاله پژوهشیمقدمه: هدف این پژوهش، بررسی تأثیر تمرین هوازی و مکمل سایلوسایبین بر بیان ژن پلیآدنوزین دی فسفات ریبوز پلیمراز (Poly (ADP-Ribose) Polymerase) PARP و گیرندهی دوپامین ۵ (D5R) در بافت قلب موشهای صحرایی معتاد به متآمفتامین بود.روشها: مطالعهی حاضر به روش تجربی با طرح پسآزمون با گروه شاهد انجام شد. ۳۰ موش صحرایی ماده نژاد ویستار بهطور تصادفی در پنج گروه شامل: کنترل سالم؛ متآمفتامین؛ متآمفتامین+ فعالیت هوازی؛ متآمفتامین+ سایلوسایبین؛ متآمفتامین+ فعالیتهوازی+ سایلوسایبین تقسیمبندی شدند. تزریق درون صفاقی متآمفتامین و سایلوسایبین به ترتیب ۱۰ و 0/02 میلیگرم بر کیلوگرم بود. مداخلات مصرف سایلوسایبین و تمرینات هوازی به مدت 4 هفته در دوره ترک اجرا شد. برای سنجش بیان ژنها در بافت قلب از روشReal Time PCR استفاده شد. دادهها با استفاده از آزمونهای آماری T مستقل، Mann-Whitney و Kruskal-Wallis، تحلیل گردید.یافتهها: مصرف متآمفتامین، سبب افزایش غیرمعنادار (20 درصدی) بیان ژن D5R نسبت به گروه شاهد شد اما نتوانست تغییر معنیداری در بیان ژن PARPایجاد کند. مصرف مکمل سایلوسایبین و ترکیب آن با تمرین هوازی، اثرات کاهشی اما غیرمعنیدار بر بیان ژن PARP و D5R در بافت قلب موشهای صحرایی مادهی معتاد به متآمفتامین ایجاد کرد.نتیجهگیری: ممکن است القاء کوتاهمدت متآمفتامین تغییر چندانی در بیان ژنهای PARP و D5R در قلب ایجاد نکند. مکمل سایلوسایبین و ترکیب آن با تمرین هوازی در دورهی ترک با وجود اینکه اثرات کاهشی غیرمعنیدار در بیان این ژنها ایجاد کرده اما بیان دقیق تغییرات ژنی نیازمند بررسیهای بیشتر است. - The effects of two different intensities of combined training on c1q/tnf-related protein 3 (Ctrp3) and insulin resistance in women with non-alcoholic fatty liver disease
Somayeh Rajabi, Roya Askari, Amir Hossein Haghighi, Nasrin Razavianzadeh
Hepatitis Monthly, 2021
Background: C1q/TNF-related protein (CTRP3) is a potent anti-inflammatory adipokine with activities, such as reduction of glucose level and inhibition of gluconeogenesis in the liver. However, the effect of exercise training on CTRP3 in patients with non-alcoholic fatty liver disease (NAFLD) remains unknown. Objectives: This study was done to investigate the effects of two different intensities of combined training on CTRP3 and insulin resistance in women with NAFLD and compare these two training patterns. Methods: Thirty-three women with NAFLD were randomly divided into three equal groups. Group 1 performed resistance training (RT), along with aerobic interval training (AIT) (2 - 5 intervals of four minutes, 70 - 75% HRmax), group 2 performed RT along with high-intensity interval training (HIIT) (8 - 13 intervals of one minute, 85 - 95% HRmax), and the control group did not participate in any training. The body composition measurements and blood sampling were carried out before and after 12 weeks of training. Data analysis was performed using repeated-measures ANOVA (α ≤ 0.05). Results: After 12 weeks, the CTRP3 level significantly increased in group 1 compared with the control group (P = 0.01) and group 2 (P < 0.001). The fasting glucose and fasting insulin levels significantly decreased in group 1 compared with the control group (P < 0.001 and P = 0.01, respectively). The insulin resistance index decreased in both group 1 and group 2; however, the difference was not significant compared with the control group (P > 0.05). Conclusions: Combined training (RT + AIT) in the present study increased the level of CTRP3; thus, it is likely that women with NAFLD can benefit from this program as a non-pharmacological adjunct treatment to prevent inflammation and progression of the disease. - Effect of resistance-interval training with two different intensities on cytokeratin18 and some functional parameters in women with fatty liver
Somayeh Rajabi, Roya Askari, Amir Hossein Haghighi, Nasrin Razaviyanzadeh
Iranian Journal of Obstetrics Gynecology and Infertility, 2020
Introduction: Nowadays, noninvasive approaches in the investigation of hepatocyte death and the use of non-pharmacological adjunctive therapies are considered by health professionals. This study was performed with aim to compare the effect of different intensities of combined training on cytokeratin18 )marker of hepatocyte death( and some indicators of physical function in women with Non-alcoholic fatty liver disease. Methods: This applied and semi-experimental study with pre-test and post-test design was performed on 33 women with NAFLD in Shahrood city in 2018. The subjects were randomly divided into training and control groups. Combined training 1 Group performed Aerobic Interval Training (AIT) along with resistance training and combined training 2 group performed High Intensity Interval Training (HIIT) along with resistance training for 12 weeks. Functional tests (Upper and lower strength and endurance, aerobic power) and blood samples were performed before and after 12 weeks of training. Data were analyzed by SPSS software (version 24) and R statistical software and repeated measures ANOVA tests. PResults: There was significant difference between two training groups in the CK18 level (p<0.001) and combined training 1 group had higher decrease in the CK18 level. Also, both combined training significantly increased upper and lower maximal strength and muscular endurance and VO2max, as well as a significant decrease in body fat percentage (p<0.001). There was no significant difference between groups in weight and BMI (p>0.05). Conclusion: Women with Non-alcoholic fatty liver disease may be able to use both intense and moderate combined training to reduce body fat percentage, improve physical performance indicators independent of weight loss and use moderate intensity combined training as a non-pharmacological adjunctive therapy to prevent cell death. - Effects of endurance and resistance training on calcitonin gene-related peptide and acetylcholine receptor at slow and fast twitch skeletal muscles and sciatic nerve in male wistar rats
Abdolhossein Parnow, Reza Gharakhanlou, Zeinab Gorginkaraji, Somayeh Rajabi, Rasoul Eslami, et al.
International Journal of Peptides, 2012
The aim of this study was to investigate effects of endurance and resistance training (ET and RT) on CGRP and AChRs at slow and fast twitch muscles and sciatic nerve in rats. Twenty-five male rats were randomly assigned into three groups including sedentary (SED), endurance training (ET), and resistance training (RT). Animals of ET exercised for 12 weeks, five times/week, and 60 min/day at 30 m/min. Animals of RT were housed in metal cage with 2 m high wire-mesh tower, with water bottles set at the top. 48 h after the last session of training protocol, animals were anaesthetized. The right sciatic nerves were removed; then, Soleus (SOL) and Tibialis anterior (TA) muscles were excised and immediately snap frozen in liquid nitrogen. All frozen tissues were stored at −80°C. Results showed that, after both ET and RT, CGRP content as well as AChR content of SOL and TA muscles significantly increased. But there was no significant difference among groups at sciatic nerve’ CGRP content. In conclusion, data demonstrate that ET and RT lead to changes of CGRP and AChR content of ST and FT muscles. The changes indicate to the importance of neuromuscular activity. - Effects of endurance and resistance training on calcitonin gene-related peptide content in slow and fast twitch rat muscles
Iranian Journal of Endocrinology and Metabolism, 2009
