Novel CRISPR/Cas12-based assay for the rapid and accurate detection of donkey meat Mohammed Molaghi, Wisam Atiyah, Asseel Saeed Open Veterinary Journal, 2025 Background: Precise identification of items originating from animals is required for monitoring the authenticity of livestock commodities. Nucleic acid-based detection methods, including polymerase chain reaction, are highly accurate tests for detecting meat fraud. However, these tests require costly devices and highly skilled personnel. Aim: This study aims to develop a precise and rapid test based on deoxyribonucleic acid (DNA) for detecting meat from donkeys. Methods: The assay was developed by combining Clustered regularly interspaced short palindromic repeat /Cas12 with RPA and a lateral flow device. A conserved sequence of the mitochondrial D-loop gene was used as the target gene. The assessment of the assay focused on its sensitivity, specificity, and completion time. Results: The results show that the assay can detect donkey meat in the tested samples with 100% specificity with no cross-reactivity with other species, and no false-positive results were recorded. Furthermore, the assay has shown high sensitivity and to detect as little as 5 ng of input DNA. Moreover, the results, including DNA extraction, were obtained in less than 40 minutes and did not require any specialized equipment. Conclusion: The assay is a good option for the rapid and precise detection of donkey meat. Further optimization and studies are needed to enable the direct detection of donkey meat without DNA extraction.
The cytolytic capacity of Enterococcus faecalis isolates from cheese, yogurt, and curd Wisam Raheem Atiyah, Orooba Meteab Faja, Qasim Zamil Bneed, Ziad M.F. Alkhozai M.F. Alkhozai Iraqi Journal of Veterinary Sciences, 2024 Food safety is an important issue that concerns scientific and commercial communities worldwide for its direct impact on people’s health. The current study used conventional methods to isolate Enterococcus faecalis from dairy products. The study was also evaluated its pathogenicity (cytolysin) and detected the genes responsible for the production of cytolysin (cylM, cylB, asa, cylA, and cylLs) using polymerase chain reaction (PCR). Here, the study was first started with collecting of 72 dairy product samples (21 cheese, 31 yogurts, and 20 curd samples). The samples were subjected to conventional cultivation, cytotoxicity, and PCR methods. The results of the cultivation revealed the presence of the E. faecalis in 43/72 (59.7%) samples, which is distributed over 20/21 (95.2%) cheese, 14/31 (45.2%) yogurt, and 9/20 (45%) curd samples. The findings of the hemolysis analysis demonstrated that the hemolytic capability of the isolated bacteria of types (γ, α, and β) differed, depending on the type of blood agar (BA) employed (human (Hn), sheep (S), and horse (Hs)). On HnBA plates, 17 isolates (39.5%) exhibited γ hemolytic action; however, 21 isolates (48.8%) exhibited β hemolytic activity. The cytotoxic activity on Vero cells showed various rates of dead cells and ranged from 0.2% (for the control) to 50.1% for specific isolates. The result of the PCR revealed the amplification of the examined genes in the bacterial isolates of dairy products. The current data demonstrate the presence of Enterococcus faecalis in dairy products with the indications of its pathogenicity due to the presence of cytolysin-coding genes and the cytotoxic activity of this protein on Vero cells.
Molecular genotyping of Salmonella spp. isolated from cheese samples of local stores in Al-Diwaniyah city, Iraq Orooba Faja, Afrah Mhyson, Wisam Atiyah, Basima Mohammed, Azal Adnan Open Veterinary Journal, 2023 Background: Food safety is an important subject that global cheese industry increases the awareness upon. This urges these economic sectors to elevate the level of research to minimize cheese contamination with pathogenic bacteria, such as Salmonella. Aim: Based on these merits, this study was conducted to genotype Salmonella spp isolated from cheese samples of local stores in Al-Diwaniyah City, Iraq. Methods: The study used 41 samples of local fresh unsalted white cheese in a selective-growth based isolation of Salmonella. These isolates were confirmed utilizing a slide-agglutination (SA) test and VITEK® 2 system (V2S). Then, the isolates were subjected to conventional PCR and sequencing techniques that both targeted the 16S rRNA gene. For subtyping, the Salmonella isolates were subjected to a random amplified polymorphic DNA (RAPD)-PCR method. Results: The results of both SA and V2S revealed the presence of 14 (34.2%) isolates of Salmonella spp. in the cheese samples. The PCR confirmed 6 (42.9%) of these isolates, which further were defined with close nucleotide similarity (98.03%) and (97.88%) to different world isolates, such as Salmonella enterica subsp. Arizonae and Salmonella enterica subsp. enterica serovar Typhi, respectively. The RAPD-PCR findings showed different fragments for all the tested isolates. Conclusion: The present study indicates that the samples of the local fresh unsalted white cheese contain different Salmonella genotypes, which could be originated from different contamination sources.
Enhancing the productive performance of broiler chickens by adding Spirulina platensis compared with probiotic, prebiotics, and oxytetracycline Wisam R Atiyah, Mohanad F Hamood Iraqi Journal of Veterinary Medicine, 2021 This study was conducted in order to find out the effect of Spirulina platensis (S. platensis) algae mixed with drinking water (2%) on the production performance of broiler chickens infected with or without Enterococcus faecalis (E. faecalis) and compare it with the effects of probiotics (containing Lactobacillus acidophilus, L. casi, L. reuteri, and Bacillus subtillis), prebiotics (containing antioxidants and a group of vitamins) as well as oxytetracycline. The experiment included 350 one day old (Ross-308) broiler chicks, randomly divided into 7 groups by 50 chicks per group for 35 days. The groups were divided as follows: T1: infected experimentally with E. faecalis only, T2: treated with S. platensis, T3: infected experimentally with E. faecalis and treated with 2% S. platensis, T4: infected experimentally with E. faecalis and treated with probiotic, T5: infected experimentally with E. faecalis and treated with prebiotic, T6: infected experimentally with E. faecalis and treated with oxytetracycline, and T7: control group without any addition. Weekly live body weight and weekly body weight gain were measured as well as weekly feed intake and feed conversion rate for broiler chickens were estimated. The current study proved that S. platensis algae added to drinking water (2%) in T2 and T3 groups had caused a significant increase (P<0.05) in the weekly live body weight and weekly body weight gain, Spirulina also caused a significant decrease (P<0.05) in weekly feed intake and feed conversion rate for broiler chickens. Therefore, S. platensis could be a suitable alternative to some feed additives such as probiotics, prebiotics, and antibiotics in addition to the ability of Spirulina to reduce the symptoms of E. faecalis.
Genotypic analysis of ticks species infesting cattle in al-diwaniyah abattoir Mansoor J. Ali, Wisam R. Atiyah, Monyer A. Al-Fatlawi, Saba F. Khlaif Iraqi Journal of Veterinary Sciences, 2021 Different vectors are considered critical for disease transmission between animals; however, ticks play a significant role in the dissemination of various infectious illnesses of animals and human importance. The current work was carried out to categorize ticks genetically of those isolated from cattle that entered Al-Diwaniyah abattoir. In the present study, 50 tick samples were collected and subjected to microscopic examination and genetic-based methods of polymerase chain reaction and partial gene sequencing, both utilized the mitochondrial cytochrome c oxidase subunit I (COX1) gene as a genotyping element. The findings of the microscopic examination showed that the ticks were from Hyalomma spp. Further analysis, the polymerase chain reaction revealed the genus of Hyalomma of the ticks, but when the PGS was performed, one sample of H. detritum, three samples of H. excavatum, and two samples of H. marginatum were identified. When the phylogenetic analyses were conducted, H. detritum showed close genetic similarity to an isolate from Spain EU827695.1. H. excavatum revealed similarity with isolates from India MK863382.1 and Turkey MT230050.1. In contrast, H. marginatum displayed close identity to an isolate from Iran (MG557555.1). In conclusion, these findings may indicate evolutionary links of the locally identified isolates to different world isolates, probably due to the trade-moving of animals.
EFFECT OF SPIRULINA PLATENSIS ON BLOOD PARAMETERS AND SOME MEAT QUALITY TRAITS IN BROILER CHICKENS Biochemical and Cellular Archives, 2021
Novel CRISPR/Cas12-based assay for the rapid and accurate detection of donkey meat MAA Molaghi, WR Atiyah, AAR Saeed Open Veterinary Journal 15 (11), 5682 , 2025 2025
The cytolytic capacity of Enterococcus faecalis isolates from cheese, yogurt, and curd ZMA W.R. Atiyah, O.M. Faja, Q.Z. Bneed Iraqi Journal of Veterinary Sciences , 2024 2024
Molecular genotyping of Salmonella spp. isolated from cheese samples of local stores in Al-Diwaniyah city, Iraq OM Faja, AS Mhyson, WR Atiyah, BJ Mohammed, A Adnan Open Veterinary Journal 13 (10), 1277–1282-1277–1282 , 2023 2023 Citations: 3
Assessment of malondialdehyde level in broiler chickens suffering from chronic heat stress and fed on a diet supplemented with bioactive substances KH Abass, WR Atiyah, MA Mlaghee International Journal of Health Sciences 6 (S9), 1714-1720 , 2022 2022 Citations: 1
Genotypic analysis of ticks species infesting cattle in al-diwaniyah abattoir. MJ Ali, WRR Atiyah, MAA Al-Fatlawi, SF Khlaif 2021 Citations: 15
Effect of Spirulina platensis on blood parameters and some meat quality traits in broiler chickens. WR Atiyah, MF Hamood Biochemical & Cellular Archives 21 (1) , 2021 2021
Enhancing the productive performance of broiler chickens by adding Spirulina platensis compared with probiotic, prebiotics, and oxytetracycline W Atyiah The Iraqi Journal of Veterinary Medicine , 2021 2021 Citations: 13
The Effect of Rehabilitation Exercises and Electric Muscle Stimulation (X Body) on Optimizing Leg Connective Muscles Strength and Biomechanical variables for Injured Athletes W kassim Jawad, LK Mohammed, WF Atiyah International Journal of Psychosocial Rehabilitation 24 (2), 216-223 , 2020 2020 Citations: 3
ANTIBACTERIAL ACTIVITY OF PAPAYA LEAF EXTRACTS AGAINST SOME PATHOGENIC BACTERIA ISOLATED FROM CHEESE IN AL-DIWANIYAH CITY, IRAQ AASOMF Wisam Raheem Atiyah1, Khilood Hamdan Fahad2 Biochemical and Cellular Archives 20 (1), 1833-1837 , 2020 2020
Genotypic Study of Hepatitis Type B in Al-Diwaniya Province, Iraq. WS Abbood, HS Atiyah Al-Mhanah Indian Journal of Public Health Research & Development 10 (6) , 2019 2019 Citations: 5
PROBIOTIC ANTAGONISTIC EFFECTS OF Lactobacillus collinoides FROM COW MILK ON COW-DIARRHEA-RECOVERED ENTEROTOXIGENIC E. coli: AN IN VIVO STUDY AS Mhyson, WR Atiyah, A Abd Sharad Vivo , 2019 2019 Citations: 1
MOST CITED SCHOLAR PUBLICATIONS
Genotypic analysis of ticks species infesting cattle in al-diwaniyah abattoir. MJ Ali, WRR Atiyah, MAA Al-Fatlawi, SF Khlaif 2021 Citations: 15
Enhancing the productive performance of broiler chickens by adding Spirulina platensis compared with probiotic, prebiotics, and oxytetracycline W Atyiah The Iraqi Journal of Veterinary Medicine , 2021 2021 Citations: 13
Genotypic Study of Hepatitis Type B in Al-Diwaniya Province, Iraq. WS Abbood, HS Atiyah Al-Mhanah Indian Journal of Public Health Research & Development 10 (6) , 2019 2019 Citations: 5
Molecular genotyping of Salmonella spp. isolated from cheese samples of local stores in Al-Diwaniyah city, Iraq OM Faja, AS Mhyson, WR Atiyah, BJ Mohammed, A Adnan Open Veterinary Journal 13 (10), 1277–1282-1277–1282 , 2023 2023 Citations: 3
The Effect of Rehabilitation Exercises and Electric Muscle Stimulation (X Body) on Optimizing Leg Connective Muscles Strength and Biomechanical variables for Injured Athletes W kassim Jawad, LK Mohammed, WF Atiyah International Journal of Psychosocial Rehabilitation 24 (2), 216-223 , 2020 2020 Citations: 3
Assessment of malondialdehyde level in broiler chickens suffering from chronic heat stress and fed on a diet supplemented with bioactive substances KH Abass, WR Atiyah, MA Mlaghee International Journal of Health Sciences 6 (S9), 1714-1720 , 2022 2022 Citations: 1
PROBIOTIC ANTAGONISTIC EFFECTS OF Lactobacillus collinoides FROM COW MILK ON COW-DIARRHEA-RECOVERED ENTEROTOXIGENIC E. coli: AN IN VIVO STUDY AS Mhyson, WR Atiyah, A Abd Sharad Vivo , 2019 2019 Citations: 1
Novel CRISPR/Cas12-based assay for the rapid and accurate detection of donkey meat MAA Molaghi, WR Atiyah, AAR Saeed Open Veterinary Journal 15 (11), 5682 , 2025 2025
The cytolytic capacity of Enterococcus faecalis isolates from cheese, yogurt, and curd ZMA W.R. Atiyah, O.M. Faja, Q.Z. Bneed Iraqi Journal of Veterinary Sciences , 2024 2024
Effect of Spirulina platensis on blood parameters and some meat quality traits in broiler chickens. WR Atiyah, MF Hamood Biochemical & Cellular Archives 21 (1) , 2021 2021
ANTIBACTERIAL ACTIVITY OF PAPAYA LEAF EXTRACTS AGAINST SOME PATHOGENIC BACTERIA ISOLATED FROM CHEESE IN AL-DIWANIYAH CITY, IRAQ AASOMF Wisam Raheem Atiyah1, Khilood Hamdan Fahad2 Biochemical and Cellular Archives 20 (1), 1833-1837 , 2020 2020
