Crystal structure-guided revelation of metal ion-dependent functional ambiguity in Pseudomonas aeruginosa histidinol dehydrogenase Gourab Basu Choudhury, Rakesh Chatterjee, Angira Saha, Dhruba Jyoti Sarkar, Basanta Kumar Das, Saumen Datta FEBS Journal, 2025 Histidinol dehydrogenase (HisD) is an enzyme that catalyzes the final step in histidine biosynthesis, converting l‐histidinol to l‐histidine, and plays a crucial role in bacterial metabolism. In this study, we investigated the ambiguity in catalytic mechanisms of the HisD enzyme in Pseudomonas aeruginosa using biochemical and structural approaches, particularly through X‐ray crystallography. The primary objective of this research was to explore the structural and functional variability of PaHisD and provide knowledge for potential therapeutic developments in this organism. Our findings reveal significant structural alterations in the enzyme as we identified a new substrate‐binding pocket due to structural rearrangements. We also confirmed the presence of an additional metal ion (Zn2+), contributing to its catalytic ambiguity. Given its relevance in molecular drug targeting, we examined how the differences in NAD+ and substrate binding could impact the efficacy of existing inhibitors. Computational studies further evaluated the variability in inhibitor binding, providing new insights for designing more effective therapeutic agents targeting PaHisD.
Sensors for Microplastics Detection in Environmental Matrices DJ Sarkar, S Mondal, R Raja, DK Yadav, SD Sarkar, SV Kumar, BK Das, ... Current Pollution Reports 12 (1), 17 , 2026 2026
Population pharmacokinetics, in-feed bioavailability and tissue depletion of imidazole antifungal clotrimazole in Pangasianodon hypophthalmus SK Rathod, SK Manna, G Tripathi, BK Das, DJ Sarkar, N Das, S Kumar, ... Veterinary Research Communications 50 (3), 220 , 2026 2026
Smartphone-integrated colorimetric aptamer-based assay employing peroxidase-mimetic gold nanozymes for oxytetracycline detection in fish muscle DJ Sarkar, R Raja, S Bhattacharyya, S Pal, S Mukherjee, BK Das Methods , 2026 2026
Ecological risk assessment of microplastics contamination in water, sediment and native fish species of the Brahmaputra River, India DJ Sarkar, SK Jatav, R Kumar, SD Sarkar, S Borah, A Saha, SK Vanniaraj, ... Journal of Contaminant Hydrology, 104946 , 2026 2026
Distribution and mobility of heavy metals in the sediments of a highly urbanized tropical estuary (Netravathi–Gurupur estuary, southwestern coast, India) S. Sahoo et al. S Sahoo, A Saha, DJ Sarkar, MF Khan, ME Vijaykumar, S Samanta, ... Environmental Geochemistry and Health 48 (5), 238 , 2026 2026
Medicinal Plants and Phytochemicals used in Diabetic Nephropathy: A Comprehensive Review S Bhattacharyya, D sarkar Authorea 2026 (0316) , 2026 2026
Acute Toxicity and Safe Level of Hexavalent Chromium to Euryhaline Milkfish in Freshwater Synergising with Morphological, Behavioural and Histological Indices K Nagendrasai, KK Krishnani, AK Verma, DJ Sarkar, PA Patil, N Poojary, ... Bulletin of Environmental Contamination and Toxicology 116 (1), 18 , 2026 2026
Microplastics abundance in major tributaries and distributaries of the river Ganga at lower estuarine stretch BK Das, R Besra, S Das, DJ Sarkar, D Mohanty, S Roy, B Baisakhi, ... Marine Pollution Bulletin 222, 118715 , 2026 2026
Peroxidase mimic feroxyhyte (FeOOH) nanoparticles enabled highly specific colorimetric detection of arsenate in water and fish DJ Sarkar, R Raja, BK Das, S Bhattacharyya, S Pal, S Mukherjee Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 339, 126299 , 2025 2025 Citations: 4
Tracing source of microplastics contamination in CTC tea: Effect of processing stages and human health risk assessment R Mondal, DJ Sarkar, S Bhattacharyya, P Chaudhuri, BK Das Journal of Hazardous Materials, 140111 , 2025 2025 Citations: 4
ROPIVACAINE VERSUS LEVOBUPIVACAINE IN USG GUIDED TAP BLOCK FOR POST OPERATIVE ANALGESIA IN PATIENTS UNDERGOING INFRAUMBILICAL ABDOMINAL SURGERY UNDER GENERAL ANAESTHESIA S Ganguly, N Biswas, D Sarkar International Journal of Medicine and Public Health 15 (4), 3029-3034 , 2025 2025
Microplastics Pollution in the Brahmaputra River and its Associated Ecosystems: a Transboundary Perspective Through Meta-Analysis SK Jatav, DJ Sarkar, BK Das, S Das Sarkar, SK Vanniaraj, R Kumar, ... Water, Air, & Soil Pollution 236 (10), 672 , 2025 2025 Citations: 3
Health risk assessment of microplastics contamination in the daily diet of South Asian countries R Mondal, DJ Sarkar, S Bhattacharyya, R Raja, P Chaudhuri, JK Biswas, ... Journal of Hazardous Materials 494, 138527 , 2025 2025 Citations: 14
Biopolymer-encapsulated calcium peroxide tablets for slow sustained release of dissolved oxygen in fish tank A Das, Y Sun, DJ Sarkar, VS Kumar, BK Das Aquacultural Engineering 110, 102524 , 2025 2025 Citations: 5
Crystal structure-guided revelation of metal ion-dependent functional ambiguity in Pseudomonas aeruginosa histidinol dehydrogenase G Basu Choudhury, R Chatterjee, A Saha, DJ Sarkar, BK Das, S Datta The FEBS Journal, 10.1111/febs.70209 , 2025 2025
Tiny Gangetic titans: investigating trace elements profiles in small indigenous fishes S Ganguly, BK Das, DJ Sarkar, C Nirupada, S Dutta Science and Culture 91, 459-461 , 2025 2025
Evaluation of potentially toxic elements in Gangetic fish: an environmental and health assessment perspectives S Roy, A Biswas, S Jana, L Chakraborty, DJ Sarkar, BK Das, PK Parida Science and Culture 91, 380-386 , 2025 2025
Introduction to Microplastics: A Global Perspective of an Alarming Contaminant in the Aquatic Ecosystem S Roy, DJ Sarkar, K Mondal, BK Das Laboratory Techniques for Fish Disease Diagnosis, 539-554 , 2025 2025 Citations: 2
Breaking barrier of binding buffer in colorimetric aptasensing of tetracycline in food fish using peroxidase mimic gold NanoZyme DJ Sarkar, R Raja, V Santhana Kumar, S Bhattacharyya, S Pal, ... Methods , 2025 2025 Citations: 1
Utilizing microalgae biofilm for reducing arsenic toxicity on fish grown in contaminated waters: An innovative solution for safer and cleaner aquaculture in arsenic endemic areas VS Kumar, DJ Sarkar, BK Das, S Samanta, G Tripathi, SD Sarkar, SK Nag Aquaculture 599, 742121 , 2025 2025 Citations: 10
MOST CITED SCHOLAR PUBLICATIONS
Spatial distribution of meso and microplastics in the sediments of river Ganga at eastern India DJ Sarkar, SD Sarkar, BK Das, RK Manna, BK Behera, S Samanta Science of the Total Environment 694, 133712 , 2019 2019 Citations: 339
Occurrence, fate and removal of microplastics as heavy metal vector in natural wastewater treatment wetland system DJ Sarkar, S Sarkar Das, BK Das, BK Sahoo, A Das, SK Nag, RK Manna, ... Water Research , 2021 2021 Citations: 292
Microplastics removal efficiency of drinking water treatment plant with pulse clarifier DJ Sarkar, SD Sarkar, BK Das, JK Praharaj, DK Mahajan, B Purokait, ... Journal of Hazardous Materials 413, 125347 , 2021 2021 Citations: 221
Antifungal activity of nano emulsions of neem and citronella oils against phytopathogenic fungi, Rhizoctonia solani and Sclerotium rolfsii JK Eisa Osman Mohamed Ali, Najam Akhtar Shakil⁎ , Virendra Singh Rana ... Industrial Crop and Products 108, 379-387 , 2017 2017 Citations: 211
Polycyclic Aromatic Hydrocarbons (PAHs) in inland aquatic ecosystems: Perils and remedies through biosensors and bioremediation BK Behera, A Das, DJ Sarkar, P Weerathunge, PK Parida, BK Das, ... Environmental Pollution 241, 212-233 , 2018 2018 Citations: 200
Metagenomic analysis reveals bacterial and fungal diversity and their bioremediation potential from sediments of river Ganga and Yamuna in India BK Behera, HJ Chakraborty, B Patra, AK Rout, B Dehury, BK Das, ... Frontiers in microbiology 11, 556136 , 2020 2020 Citations: 120
Nanoemulsion based alginate organic coating for shelf life extension of okra DS Gajanan Gundewadia, Shalini Gaur Rudraa, Dhruba Jyoti Sarkarb Food Packaging and Shelf Life 18, 1-12 , 2018 2018 Citations: 111
Base triggered release of insecticide from bentonite reinforced citric acid crosslinked carboxymethyl cellulose hydrogel composites DJ Sarkar, A Singh Carbohydrate Polymers (http://dx.doi.org/10.1016/j.carbpol.2016.09.045) , 2017 2017 Citations: 105
Release kinetics of controlled release formulations of thiamethoxam employing nano-ranged amphiphilic PEG and diacid based block polymers in soil DJ Sarkar, J Kumar, NA Shakil, S Walia Journal of Environmental Science and Health, Part A 47 (11), 1701-1712 , 2012 2012 Citations: 96
Encyclopedia of Polymer Applications, 3 Volume Set M Mishra CRC press , 2018 2018 Citations: 92
Studies on novel nanosuperabsorbent composites: Swelling behavior in different environments and effect on water absorption and retention properties of sandy loam soil and soil … A Singh, DJ Sarkar, AK Singh, R Parsad, A Kumar, BS Parmar Journal of applied polymer science 120 (3), 1448-1458 , 2011 2011 Citations: 88
pH‐sensitive crosslinked guar gum‐based superabsorbent hydrogels: Swelling response in simulated environments and water retention behavior in plant growth media KSVP Chandrika, A Singh, DJ Sarkar, A Rathore, A Kumar Journal of Applied Polymer Science 131 (22) , 2014 2014 Citations: 65
Taxonomic profiling and functional gene annotation of microbial communities in sediment of river Ganga at Kanpur, India: insights from whole-genome metagenomics study AK Rout, B Dehury, PK Parida, DJ Sarkar, B Behera, BK Das, A Rai, ... Environmental Science and Pollution Research 29 (54), 82309-82323 , 2022 2022 Citations: 64
Preparation of basil oil nanoemulsion using Sapindus mukorossi pericarp extract: Physico-chemical properties and antifungal activity against food spoilage pathogens DS Gajanan Gundewadi, Dhruba Jyoti Sarkar, Shalini Gaur Rudra Industrial Crops and Products 125, 95-104 , 2018 2018 Citations: 64
Metagenome analysis from the sediment of river Ganga and Yamuna: In search of beneficial microbiome BK Behera, B Patra, HJ Chakraborty, P Sahu, AK Rout, DJ Sarkar, ... PloS one 15 (10), e0239594 , 2020 2020 Citations: 63
Community structure and function of microbiomes in polluted stretches of river Yamuna in New Delhi, India, using shotgun metagenomics PK Parida, BK Behera, B Dehury, AK Rout, DJ Sarkar, A Rai, BK Das, ... Environmental Science and Pollution Research 29 (47), 71311-71325 , 2022 2022 Citations: 55
Metagenomic landscape of sediments of river Ganga reveals microbial diversity, potential plastic and xenobiotic degradation enzymes AK Rout, S Dixit, PS Tripathy, SS Rout, SN Parida, PK Parida, DJ Sarkar, ... Journal of Hazardous Materials 471, 134377 , 2024 2024 Citations: 52
Metagenomics study in aquatic resource management: recent trends, applied methodologies and future needs BK Behera, B Dehury, AK Rout, B Patra, N Mantri, HJ Chakraborty, ... Gene Reports 25, 101372 , 2021 2021 Citations: 48
Synthesis and characterization of poly (CMC-g-cl-PAam/Zeolite) superabsorbent composites for controlled delivery of zinc micronutrient: swelling and release behavior DJ Sarkar, A Singh, P Mandal, A Kumar, BS Parmar Polymer-Plastics Technology and Engineering 54 (4), 357-367 , 2015 2015 Citations: 44
Agriculture: Polymers in crop production mulch and fertilizer DJ Sarkar, M Barman, T Bera, M De, D Chatterjee Encyclopedia of polymer applications 1, 1-20 , 2018 2018 Citations: 42